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Entry version 128 (13 Feb 2019)
Sequence version 1 (01 Feb 1994)
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Protein

DNA primase large subunit

Gene

Prim2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi287Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi367Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi384Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi424Iron-sulfur (4Fe-4S)By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • DNA primase activity Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • single-stranded DNA binding Source: GO_Central

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-MMU-174411 Polymerase switching on the C-strand of the telomere
R-MMU-174430 Telomere C-strand synthesis initiation
R-MMU-68952 DNA replication initiation
R-MMU-68962 Activation of the pre-replicative complex
R-MMU-69091 Polymerase switching
R-MMU-69166 Removal of the Flap Intermediate
R-MMU-69183 Processive synthesis on the lagging strand

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA primase large subunit (EC:2.7.7.-)
Alternative name(s):
DNA primase 58 kDa subunit
Short name:
p58
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Prim2
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 1

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:97758 Prim2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000467691 – 505DNA primase large subunitAdd BLAST505

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei470PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...
EPDi
P33610

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P33610

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P33610

PeptideAtlas

More...
PeptideAtlasi
P33610

PRoteomics IDEntifications database

More...
PRIDEi
P33610

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P33610

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
P33610

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000026134 Expressed in 302 organ(s), highest expression level in respiratory system

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
P33610 MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer of a small subunit and a large subunit.

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
202362, 4 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-2088 DNA polymerase alpha:primase complex

CORUM comprehensive resource of mammalian protein complexes

More...
CORUMi
P33610

Protein interaction database and analysis system

More...
IntActi
P33610, 6 interactors

STRING: functional protein association networks

More...
STRINGi
10090.ENSMUSP00000027312

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
P33610

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P33610

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2267 Eukaryota
COG2219 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000009790

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000212154

The HOVERGEN Database of Homologous Vertebrate Genes

More...
HOVERGENi
HBG008257

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P33610

KEGG Orthology (KO)

More...
KOi
K02685

Identification of Orthologs from Complete Genome Data

More...
OMAi
DCHGCPY

Database of Orthologous Groups

More...
OrthoDBi
479347at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P33610

TreeFam database of animal gene trees

More...
TreeFami
TF105893

Family and domain databases

Conserved Domains Database

More...
CDDi
cd07322 PriL_PriS_Eukaryotic, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR016558 DNA_primase_lsu_euk
IPR007238 DNA_primase_lsu_euk/arc

The PANTHER Classification System

More...
PANTHERi
PTHR10537 PTHR10537, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04104 DNA_primase_lrg, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF009449 DNA_primase_large_subunit, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P33610-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQFSGRIRKK LRLAGDQRNA SYPHSLQFYL QPPTENISLT EFENLAFDRV
60 70 80 90 100
KLLKAIENLG VSYVKGTEQY QSKLEAEIRK LKFSYRENLE DEYEPRRRDH
110 120 130 140 150
ISHFILRLAY CQSEDLRRWF IQQEMDLLRF RFSILPKDKV QSFLKDSHLH
160 170 180 190 200
FEAISDEEKT LREQDIMASS PSLSGIKLES ESVYKVPFAD ALDLFRGRKV
210 220 230 240 250
YLEDGFAYVP LKDIVAIILN EFRATLSKAL ALTARSLPAV QSDERLQPLL
260 270 280 290 300
NHLSHSYTGQ DYSTQKNTGK ISLDQIDSLS TKSFPPCMRQ LHKALRENHH
310 320 330 340 350
LRHGGRMQYG LFLKGIGLTL EQALQFWKQE FIRGKMDPDK FDKGYSYNIR
360 370 380 390 400
HSFGKEGKRT DYTPFSCMKI ILTNPPGQGD YHGCPFRHSD AELLKQKMQS
410 420 430 440 450
YKIPASGISQ ILDLVKGNHY QVACQKYFEM THNVDDCGFS LNHPNQFFFE
460 470 480 490 500
SQRILTGGKD IKKEISQPET PQHKPSTQKT RDAASALASL DSSLEMDLEG

LEEYF
Length:505
Mass (Da):58,408
Last modified:February 1, 1994 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i36A64C77669D8C24
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti109A → G in BAA04203 (PubMed:8026492).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
D13545 mRNA Translation: BAA02745.1
D17385 mRNA Translation: BAA04203.1
AK076095 mRNA Translation: BAC36179.1
AK077760 mRNA Translation: BAC36996.1
AK153970 mRNA Translation: BAE32287.1
BC019500 mRNA Translation: AAH19500.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS14862.1

Protein sequence database of the Protein Information Resource

More...
PIRi
C46642

NCBI Reference Sequences

More...
RefSeqi
NP_032948.1, NM_008922.2

UniGene gene-oriented nucleotide sequence clusters

More...
UniGenei
Mm.27705

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSMUST00000027312; ENSMUSP00000027312; ENSMUSG00000026134

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
19076

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mmu:19076

UCSC genome browser

More...
UCSCi
uc007anq.2 mouse

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13545 mRNA Translation: BAA02745.1
D17385 mRNA Translation: BAA04203.1
AK076095 mRNA Translation: BAC36179.1
AK077760 mRNA Translation: BAC36996.1
AK153970 mRNA Translation: BAE32287.1
BC019500 mRNA Translation: AAH19500.1
CCDSiCCDS14862.1
PIRiC46642
RefSeqiNP_032948.1, NM_008922.2
UniGeneiMm.27705

3D structure databases

ProteinModelPortaliP33610
SMRiP33610
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202362, 4 interactors
ComplexPortaliCPX-2088 DNA polymerase alpha:primase complex
CORUMiP33610
IntActiP33610, 6 interactors
STRINGi10090.ENSMUSP00000027312

PTM databases

iPTMnetiP33610
PhosphoSitePlusiP33610

Proteomic databases

EPDiP33610
MaxQBiP33610
PaxDbiP33610
PeptideAtlasiP33610
PRIDEiP33610

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027312; ENSMUSP00000027312; ENSMUSG00000026134
GeneIDi19076
KEGGimmu:19076
UCSCiuc007anq.2 mouse

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
5558
MGIiMGI:97758 Prim2

Phylogenomic databases

eggNOGiKOG2267 Eukaryota
COG2219 LUCA
GeneTreeiENSGT00390000009790
HOGENOMiHOG000212154
HOVERGENiHBG008257
InParanoidiP33610
KOiK02685
OMAiDCHGCPY
OrthoDBi479347at2759
PhylomeDBiP33610
TreeFamiTF105893

Enzyme and pathway databases

ReactomeiR-MMU-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-MMU-174411 Polymerase switching on the C-strand of the telomere
R-MMU-174430 Telomere C-strand synthesis initiation
R-MMU-68952 DNA replication initiation
R-MMU-68962 Activation of the pre-replicative complex
R-MMU-69091 Polymerase switching
R-MMU-69166 Removal of the Flap Intermediate
R-MMU-69183 Processive synthesis on the lagging strand

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Prim2 mouse

Protein Ontology

More...
PROi
PR:P33610

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000026134 Expressed in 302 organ(s), highest expression level in respiratory system
GenevisibleiP33610 MM

Family and domain databases

CDDicd07322 PriL_PriS_Eukaryotic, 1 hit
InterProiView protein in InterPro
IPR016558 DNA_primase_lsu_euk
IPR007238 DNA_primase_lsu_euk/arc
PANTHERiPTHR10537 PTHR10537, 1 hit
PfamiView protein in Pfam
PF04104 DNA_primase_lrg, 1 hit
PIRSFiPIRSF009449 DNA_primase_large_subunit, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPRI2_MOUSE
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P33610
Secondary accession number(s): Q3U4Z3
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 13, 2019
This is version 128 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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