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Protein

Nickel import ATP-binding protein NikD

Gene

nikD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the ABC transporter complex NikABCDE involved in nickel import. Responsible for energy coupling to the transport system.UniRule annotation

Catalytic activityi

ATP + H2O + Ni2+(Out) = ADP + phosphate + Ni2+(In).UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

  • nickel cation transmembrane transport Source: EcoCyc

Keywordsi

Molecular functionHydrolase
Biological processIon transport, Nickel transport, Transport
LigandATP-binding, Nickel, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:NIKD-MONOMER
MetaCyc:NIKD-MONOMER

Protein family/group databases

TCDBi3.A.1.5.3 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Nickel import ATP-binding protein NikDUniRule annotation (EC:3.6.3.24UniRule annotation)
Gene namesi
Name:nikDUniRule annotation
Ordered Locus Names:b3479, JW3444
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12078 nikD

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000926201 – 254Nickel import ATP-binding protein NikDAdd BLAST254

Proteomic databases

PaxDbiP33593
PRIDEiP33593

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (NikD and NikE), two transmembrane proteins (NikB and NikC) and a solute-binding protein (NikA).UniRule annotation

Protein-protein interaction databases

BioGridi4262507, 17 interactors
DIPiDIP-10343N
IntActiP33593, 11 interactors
STRINGi316385.ECDH10B_3653

Structurei

3D structure databases

ProteinModelPortaliP33593
SMRiP33593
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 241ABC transporterUniRule annotationAdd BLAST240

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Nickel importer (TC 3.A.1.5.3) family. [View classification]UniRule annotation

Phylogenomic databases

eggNOGiENOG4106665 Bacteria
COG0444 LUCA
InParanoidiP33593
KOiK15587
PhylomeDBiP33593

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR015857 ABC_transpr_NikD
IPR014138 Nickel_NikD
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02770 nickel_nikD, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit
PS51247 NIKD, 1 hit

Sequencei

Sequence statusi: Complete.

P33593-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPQQIELRNI ALQAAQPLVH GVSLTLQRGR VLALVGGSGS GKSLTCAATL
60 70 80 90 100
GILPAGVRQT AGEILADGKP VSPCALRGIK IATIMQNPRS AFNPLHTMHT
110 120 130 140 150
HARETCLALG KPADDATLTA AIEAVGLENA ARVLKLYPFE MSGGMLQRMM
160 170 180 190 200
IAMAVLCESP FIIADEPTTD LDVVAQARIL DLLESIMQKQ APGMLLVTHD
210 220 230 240 250
MGVVARLADD VAVMSDGKIV EQGDVETLFN APKHTVTRSL VSAHLALYGM

ELAS
Length:254
Mass (Da):26,820
Last modified:August 29, 2003 - v2
Checksum:iB954C5027BFBAD99
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti177 – 198ARILD…MLLVT → GGASSICWKHYAKQCGNAAG D in CAA51662 (PubMed:7934931).CuratedAdd BLAST22
Sequence conflicti239S → T in CAA51662 (PubMed:7934931).Curated1
Sequence conflicti251E → D in CAA51662 (PubMed:7934931).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73143 Genomic DNA Translation: CAA51662.1
U00039 Genomic DNA Translation: AAB18454.1
U00096 Genomic DNA Translation: AAC76504.1
AP009048 Genomic DNA Translation: BAE77814.1
PIRiS47698
RefSeqiNP_417936.1, NC_000913.3
WP_001136229.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76504; AAC76504; b3479
BAE77814; BAE77814; BAE77814
GeneIDi947989
KEGGiecj:JW3444
eco:b3479
PATRICifig|1411691.4.peg.3246

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73143 Genomic DNA Translation: CAA51662.1
U00039 Genomic DNA Translation: AAB18454.1
U00096 Genomic DNA Translation: AAC76504.1
AP009048 Genomic DNA Translation: BAE77814.1
PIRiS47698
RefSeqiNP_417936.1, NC_000913.3
WP_001136229.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP33593
SMRiP33593
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262507, 17 interactors
DIPiDIP-10343N
IntActiP33593, 11 interactors
STRINGi316385.ECDH10B_3653

Protein family/group databases

TCDBi3.A.1.5.3 the atp-binding cassette (abc) superfamily

Proteomic databases

PaxDbiP33593
PRIDEiP33593

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76504; AAC76504; b3479
BAE77814; BAE77814; BAE77814
GeneIDi947989
KEGGiecj:JW3444
eco:b3479
PATRICifig|1411691.4.peg.3246

Organism-specific databases

EchoBASEiEB2003
EcoGeneiEG12078 nikD

Phylogenomic databases

eggNOGiENOG4106665 Bacteria
COG0444 LUCA
InParanoidiP33593
KOiK15587
PhylomeDBiP33593

Enzyme and pathway databases

BioCyciEcoCyc:NIKD-MONOMER
MetaCyc:NIKD-MONOMER

Miscellaneous databases

PROiPR:P33593

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003439 ABC_transporter-like
IPR017871 ABC_transporter_CS
IPR015857 ABC_transpr_NikD
IPR014138 Nickel_NikD
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00005 ABC_tran, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02770 nickel_nikD, 1 hit
PROSITEiView protein in PROSITE
PS00211 ABC_TRANSPORTER_1, 1 hit
PS50893 ABC_TRANSPORTER_2, 1 hit
PS51247 NIKD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNIKD_ECOLI
AccessioniPrimary (citable) accession number: P33593
Secondary accession number(s): Q2M7E2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 29, 2003
Last modified: November 7, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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