UniProtKB - P33590 (NIKA_ECOLI)
Protein
Nickel-binding periplasmic protein
Gene
nikA
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in a nickel transport system, probably represents the nickel binder.
GO - Molecular functioni
- heme binding Source: EcoliWiki
- metal cluster binding Source: EcoCyc
- nickel cation binding Source: EcoliWiki
- peptide transmembrane transporter activity Source: GO_Central
- transition metal ion binding Source: EcoliWiki
GO - Biological processi
- negative chemotaxis Source: EcoliWiki
- nickel cation transport Source: InterPro
- peptide transport Source: GO_Central
Keywordsi
Biological process | Transport |
Ligand | Nickel |
Enzyme and pathway databases
BioCyci | EcoCyc:NIKA-MONOMER MetaCyc:NIKA-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.5.3, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Nickel-binding periplasmic protein |
Gene namesi | Name:nikA Ordered Locus Names:b3476, JW3441 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm Curated
Plasma Membrane
- ATP-binding cassette (ABC) transporter complex Source: InterPro
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
- periplasmic space Source: EcoliWiki
Keywords - Cellular componenti
PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Add BLAST | 22 | |
ChainiPRO_0000031800 | 23 – 524 | Nickel-binding periplasmic proteinAdd BLAST | 502 |
Proteomic databases
jPOSTi | P33590 |
PaxDbi | P33590 |
PRIDEi | P33590 |
Interactioni
Binary interactionsi
P33590
With | #Exp. | IntAct |
---|---|---|
tufA [P0CE47] | 2 | EBI-555182,EBI-301077 |
Protein-protein interaction databases
BioGRIDi | 4262491, 49 interactors |
ComplexPortali | CPX-4348, Nickel ABC transporter complex |
DIPi | DIP-10340N |
IntActi | P33590, 1 interactor |
STRINGi | 511145.b3476 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P33590 |
SMRi | P33590 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P33590 |
Family & Domainsi
Sequence similaritiesi
Belongs to the bacterial solute-binding protein 5 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0747, Bacteria |
HOGENOMi | CLU_017028_7_5_6 |
InParanoidi | P33590 |
PhylomeDBi | P33590 |
Family and domain databases
CDDi | cd08489, PBP2_NikA, 1 hit |
InterProi | View protein in InterPro IPR011980, NikA_ABC_Ni-bd IPR030678, Peptide/Ni-bd IPR039424, SBP_5 IPR023765, SBP_5_CS IPR000914, SBP_5_dom |
PANTHERi | PTHR30290, PTHR30290, 1 hit |
Pfami | View protein in Pfam PF00496, SBP_bac_5, 1 hit |
PIRSFi | PIRSF002741, MppA, 1 hit |
TIGRFAMsi | TIGR02294, nickel_nikA, 1 hit |
PROSITEi | View protein in PROSITE PS01040, SBP_BACTERIAL_5, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P33590-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSTLRRTLF ALLACASFIV HAAAPDEITT AWPVNVGPLN PHLYTPNQMF
60 70 80 90 100
AQSMVYEPLV KYQADGSVIP WLAKSWTHSE DGKTWTFTLR DDVKFSNGEP
110 120 130 140 150
FDAEAAAENF RAVLDNRQRH AWLELANQIV DVKALSKTEL QITLKSAYYP
160 170 180 190 200
FLQELALPRP FRFIAPSQFK NHETMNGIKA PIGTGPWILQ ESKLNQYDVF
210 220 230 240 250
VRNENYWGEK PAIKKITFNV IPDPTTRAVA FETGDIDLLY GNEGLLPLDT
260 270 280 290 300
FARFSQNPAY HTQLSQPIET VMLALNTAKA PTNELAVREA LNYAVNKKSL
310 320 330 340 350
IDNALYGTQQ VADTLFAPSV PYANLGLKPS QYDPQKAKAL LEKAGWTLPA
360 370 380 390 400
GKDIREKNGQ PLRIELSFIG TDALSKSMAE IIQADMRQIG ADVSLIGEEE
410 420 430 440 450
SSIYARQRDG RFGMIFHRTW GAPYDPHAFL SSMRVPSHAD FQAQQGLADK
460 470 480 490 500
PLIDKEIGEV LATHDETQRQ ALYRDILTRL HDEAVYLPIS YISMMVVSKP
510 520
ELGNIPYAPI ATEIPFEQIK PVKP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73143 Genomic DNA Translation: CAA51659.1 U00039 Genomic DNA Translation: AAB18451.1 U00096 Genomic DNA Translation: AAC76501.1 AP009048 Genomic DNA Translation: BAE77817.1 |
PIRi | S39594 |
RefSeqi | NP_417933.1, NC_000913.3 WP_000953361.1, NZ_SSZK01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC76501; AAC76501; b3476 BAE77817; BAE77817; BAE77817 |
GeneIDi | 947981 |
KEGGi | ecj:JW3441 eco:b3476 |
PATRICi | fig|1411691.4.peg.3249 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73143 Genomic DNA Translation: CAA51659.1 U00039 Genomic DNA Translation: AAB18451.1 U00096 Genomic DNA Translation: AAC76501.1 AP009048 Genomic DNA Translation: BAE77817.1 |
PIRi | S39594 |
RefSeqi | NP_417933.1, NC_000913.3 WP_000953361.1, NZ_SSZK01000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UIU | X-ray | 1.85 | A/B | 23-524 | [»] | |
1UIV | X-ray | 1.95 | A/B | 23-524 | [»] | |
1ZLQ | X-ray | 1.80 | A/B | 23-524 | [»] | |
2NOO | X-ray | 1.65 | A | 23-524 | [»] | |
3DP8 | X-ray | 2.50 | A/B/C | 23-524 | [»] | |
3E3K | X-ray | 2.80 | A/B/C | 23-524 | [»] | |
3MVW | X-ray | 1.79 | A/B | 23-524 | [»] | |
3MVX | X-ray | 1.70 | A/B | 23-524 | [»] | |
3MVY | X-ray | 2.50 | A/B | 23-524 | [»] | |
3MVZ | X-ray | 1.70 | A/B | 23-524 | [»] | |
3MW0 | X-ray | 2.30 | A/B | 23-524 | [»] | |
3MZ9 | X-ray | 1.80 | A/B | 23-524 | [»] | |
3MZB | X-ray | 1.70 | A/B | 23-523 | [»] | |
3QIM | X-ray | 2.10 | A/B | 23-524 | [»] | |
4DCX | X-ray | 2.00 | A/B | 23-524 | [»] | |
4DCY | X-ray | 2.00 | A/B | 23-524 | [»] | |
4I8C | X-ray | 2.50 | A/B/C | 23-524 | [»] | |
4I9D | X-ray | 1.70 | A/B | 23-524 | [»] | |
5L8D | X-ray | 1.80 | A/B | 23-524 | [»] | |
5MWU | X-ray | 1.80 | A/B | 23-524 | [»] | |
5ON0 | X-ray | 1.90 | A/B | 23-524 | [»] | |
5ON1 | X-ray | 1.70 | A/B | 23-524 | [»] | |
5ON4 | X-ray | 2.30 | A/B | 23-524 | [»] | |
5ON5 | X-ray | 1.70 | A/B | 23-524 | [»] | |
5ON8 | X-ray | 1.60 | A/B | 23-524 | [»] | |
5ON9 | X-ray | 1.70 | A/B | 23-524 | [»] | |
6R4Q | X-ray | 1.90 | A/B | 23-524 | [»] | |
7A0C | X-ray | 1.90 | A/B | 23-524 | [»] | |
BMRBi | P33590 | |||||
SMRi | P33590 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262491, 49 interactors |
ComplexPortali | CPX-4348, Nickel ABC transporter complex |
DIPi | DIP-10340N |
IntActi | P33590, 1 interactor |
STRINGi | 511145.b3476 |
Chemistry databases
DrugBanki | DB03374, 3,5-Diiodotyrosine |
Protein family/group databases
TCDBi | 3.A.1.5.3, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P33590 |
PaxDbi | P33590 |
PRIDEi | P33590 |
Genome annotation databases
EnsemblBacteriai | AAC76501; AAC76501; b3476 BAE77817; BAE77817; BAE77817 |
GeneIDi | 947981 |
KEGGi | ecj:JW3441 eco:b3476 |
PATRICi | fig|1411691.4.peg.3249 |
Organism-specific databases
EchoBASEi | EB2000 |
Phylogenomic databases
eggNOGi | COG0747, Bacteria |
HOGENOMi | CLU_017028_7_5_6 |
InParanoidi | P33590 |
PhylomeDBi | P33590 |
Enzyme and pathway databases
BioCyci | EcoCyc:NIKA-MONOMER MetaCyc:NIKA-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P33590 |
PROi | PR:P33590 |
Family and domain databases
CDDi | cd08489, PBP2_NikA, 1 hit |
InterProi | View protein in InterPro IPR011980, NikA_ABC_Ni-bd IPR030678, Peptide/Ni-bd IPR039424, SBP_5 IPR023765, SBP_5_CS IPR000914, SBP_5_dom |
PANTHERi | PTHR30290, PTHR30290, 1 hit |
Pfami | View protein in Pfam PF00496, SBP_bac_5, 1 hit |
PIRSFi | PIRSF002741, MppA, 1 hit |
TIGRFAMsi | TIGR02294, nickel_nikA, 1 hit |
PROSITEi | View protein in PROSITE PS01040, SBP_BACTERIAL_5, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NIKA_ECOLI | |
Accessioni | P33590Primary (citable) accession number: P33590 Secondary accession number(s): Q2M7D9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | April 7, 2021 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families