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Protein

Vitamin K-dependent protein C

Gene

Proc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.By similarity

Miscellaneous

Calcium also binds, with stronger affinity to another site, beyond the GLA domain. This GLA-independent binding site is necessary for the recognition of the thrombin-thrombomodulin complex.

Catalytic activityi

Degradation of blood coagulation factors Va and VIIIa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei253Charge relay system1
Active sitei299Charge relay system1
Active sitei401Charge relay system1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processBlood coagulation, Hemostasis
LigandCalcium

Enzyme and pathway databases

ReactomeiR-MMU-140837 Intrinsic Pathway of Fibrin Clot Formation
R-MMU-140875 Common Pathway of Fibrin Clot Formation
R-MMU-159740 Gamma-carboxylation of protein precursors
R-MMU-159763 Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus
R-MMU-159782 Removal of aminoterminal propeptides from gamma-carboxylated proteins
R-MMU-202733 Cell surface interactions at the vascular wall
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-8957275 Post-translational protein phosphorylation

Protein family/group databases

MEROPSiS01.218

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin K-dependent protein C (EC:3.4.21.69)
Alternative name(s):
Anticoagulant protein C
Autoprothrombin IIA
Blood coagulation factor XIV
Cleaved into the following 3 chains:
Gene namesi
Name:Proc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:97771 Proc

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000002811219 – 41By similarityAdd BLAST23
ChainiPRO_000002811342 – 460Vitamin K-dependent protein CAdd BLAST419
ChainiPRO_000002811442 – 196Vitamin K-dependent protein C light chainBy similarityAdd BLAST155
ChainiPRO_0000028115199 – 460Vitamin K-dependent protein C heavy chainBy similarityAdd BLAST262
PeptideiPRO_0000028116199 – 212Activation peptideBy similarityAdd BLAST14

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei474-carboxyglutamatePROSITE-ProRule annotationBy similarity1
Modified residuei484-carboxyglutamatePROSITE-ProRule annotationBy similarity1
Modified residuei554-carboxyglutamatePROSITE-ProRule annotationBy similarity1
Modified residuei574-carboxyglutamatePROSITE-ProRule annotationBy similarity1
Disulfide bondi58 ↔ 63By similarity
Modified residuei604-carboxyglutamatePROSITE-ProRule annotationBy similarity1
Modified residuei614-carboxyglutamatePROSITE-ProRule annotationBy similarity1
Modified residuei664-carboxyglutamatePROSITE-ProRule annotationBy similarity1
Modified residuei674-carboxyglutamatePROSITE-ProRule annotationBy similarity1
Modified residuei704-carboxyglutamatePROSITE-ProRule annotationBy similarity1
Modified residuei764-carboxyglutamatePROSITE-ProRule annotationBy similarity1
Disulfide bondi91 ↔ 110By similarity
Disulfide bondi100 ↔ 105By similarity
Disulfide bondi104 ↔ 119By similarity
Modified residuei112(3R)-3-hydroxyaspartateBy similarity1
Disulfide bondi121 ↔ 130By similarity
Disulfide bondi139 ↔ 150By similarity
Disulfide bondi146 ↔ 159By similarity
Disulfide bondi161 ↔ 174By similarity
Disulfide bondi182 ↔ 319Interchain (between light and heavy chains)PROSITE-ProRule annotation
Glycosylationi214N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi238 ↔ 254By similarity
Glycosylationi290N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi354N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi372 ↔ 386By similarity
Disulfide bondi397 ↔ 425By similarity

Post-translational modificationi

The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei212 – 213Cleavage; by thrombinBy similarity2

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Gamma-carboxyglutamic acid, Glycoprotein, Hydroxylation, Zymogen

Proteomic databases

MaxQBiP33587
PaxDbiP33587
PeptideAtlasiP33587
PRIDEiP33587

PTM databases

iPTMnetiP33587
PhosphoSitePlusiP33587

Expressioni

Tissue specificityi

Plasma; synthesized in the liver.

Gene expression databases

BgeeiENSMUSG00000024386 Expressed in 85 organ(s), highest expression level in liver
CleanExiMM_PROC
GenevisibleiP33587 MM

Interactioni

Subunit structurei

Synthesized as a single chain precursor, which is cleaved into a light chain and a heavy chain held together by a disulfide bond. The enzyme is then activated by thrombin, which cleaves a tetradecapeptide from the amino end of the heavy chain; this reaction, which occurs at the surface of endothelial cells, is strongly promoted by thrombomodulin.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132226

Structurei

3D structure databases

ProteinModelPortaliP33587
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 87GlaPROSITE-ProRule annotationAdd BLAST46
Domaini96 – 131EGF-like 1PROSITE-ProRule annotationAdd BLAST36
Domaini135 – 175EGF-like 2PROSITE-ProRule annotationAdd BLAST41
Domaini213 – 449Peptidase S1PROSITE-ProRule annotationAdd BLAST237

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IJRM Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118890
HOGENOMiHOG000251821
HOVERGENiHBG013304
InParanoidiP33587
KOiK01344
OMAiLDWIHSH
OrthoDBiEOG091G0AH5
PhylomeDBiP33587
TreeFamiTF327329

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
Gene3Di4.10.740.10, 1 hit
InterProiView protein in InterPro
IPR017857 Coagulation_fac-like_Gla_dom
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR035972 GLA-like_dom_SF
IPR000294 GLA_domain
IPR012224 Pept_S1A_FX
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00008 EGF, 1 hit
PF00594 Gla, 1 hit
PF00089 Trypsin, 1 hit
PIRSFiPIRSF001143 Factor_X, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
PR00001 GLABLOOD
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00179 EGF_CA, 2 hits
SM00069 GLA, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57630 SSF57630, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 2 hits
PS50026 EGF_3, 1 hit
PS01187 EGF_CA, 1 hit
PS00011 GLA_1, 1 hit
PS50998 GLA_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P33587-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWQFRVFLLL MSTWGISSIP AHPDPVFSSS EHAHQVLRVR RANSFLEEMR
60 70 80 90 100
PGSLERECME EICDFEEAQE IFQNVEDTLA FWIKYFDGDQ CSAPPLDHQC
110 120 130 140 150
DSPCCGHGTC IDGIGSFSCS CDKGWEGKFC QQELRFQDCR VNNGGCLHYC
160 170 180 190 200
LEESNGRRCA CAPGYELADD HMRCKSTVNF PCGKLGRWIE KKRKILKRDT
210 220 230 240 250
DLEDELEPDP RIVNGTLTKQ GDSPWQAILL DSKKKLACGG VLIHTSWVLT
260 270 280 290 300
AAHCVEGTKK LTVRLGEYDL RRRDHWELDL DIKEILVHPN YTRSSSDNDI
310 320 330 340 350
ALLRLAQPAT LSKTIVPICL PNNGLAQELT QAGQETVVTG WGYQSDRIKD
360 370 380 390 400
GRRNRTFILT FIRIPLVARN ECVEVMKNVV SENMLCAGII GDTRDACDGD
410 420 430 440 450
SGGPMVVFFR GTWFLVGLVS WGEGCGHTNN YGIYTKVGSY LKWIHSYIGE
460
KGVSLKSQKL
Length:460
Mass (Da):51,818
Last modified:June 7, 2004 - v2
Checksum:i0117F26E68FCC274
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65F → L in AAK07918 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti327Q → QQ in strain: BALB/c. 1
Natural varianti392D → N in strain: BALB/c. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10445 mRNA Translation: BAA01235.1
AF034569 Genomic DNA Translation: AAC33795.1
AF318182 mRNA Translation: AAK07918.1
BC013896 mRNA Translation: AAH13896.1
D43755 Genomic DNA Translation: BAA07812.1
CCDSiCCDS29116.1
PIRiJX0210
RefSeqiNP_001036232.1, NM_001042767.3
NP_001300867.1, NM_001313938.1
XP_011245159.1, XM_011246857.2
UniGeneiMm.2786
Mm.489734

Genome annotation databases

EnsembliENSMUST00000171765; ENSMUSP00000132226; ENSMUSG00000024386
GeneIDi19123
KEGGimmu:19123
UCSCiuc008eiz.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10445 mRNA Translation: BAA01235.1
AF034569 Genomic DNA Translation: AAC33795.1
AF318182 mRNA Translation: AAK07918.1
BC013896 mRNA Translation: AAH13896.1
D43755 Genomic DNA Translation: BAA07812.1
CCDSiCCDS29116.1
PIRiJX0210
RefSeqiNP_001036232.1, NM_001042767.3
NP_001300867.1, NM_001313938.1
XP_011245159.1, XM_011246857.2
UniGeneiMm.2786
Mm.489734

3D structure databases

ProteinModelPortaliP33587
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132226

Protein family/group databases

MEROPSiS01.218

PTM databases

iPTMnetiP33587
PhosphoSitePlusiP33587

Proteomic databases

MaxQBiP33587
PaxDbiP33587
PeptideAtlasiP33587
PRIDEiP33587

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000171765; ENSMUSP00000132226; ENSMUSG00000024386
GeneIDi19123
KEGGimmu:19123
UCSCiuc008eiz.1 mouse

Organism-specific databases

CTDi5624
MGIiMGI:97771 Proc

Phylogenomic databases

eggNOGiENOG410IJRM Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118890
HOGENOMiHOG000251821
HOVERGENiHBG013304
InParanoidiP33587
KOiK01344
OMAiLDWIHSH
OrthoDBiEOG091G0AH5
PhylomeDBiP33587
TreeFamiTF327329

Enzyme and pathway databases

ReactomeiR-MMU-140837 Intrinsic Pathway of Fibrin Clot Formation
R-MMU-140875 Common Pathway of Fibrin Clot Formation
R-MMU-159740 Gamma-carboxylation of protein precursors
R-MMU-159763 Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus
R-MMU-159782 Removal of aminoterminal propeptides from gamma-carboxylated proteins
R-MMU-202733 Cell surface interactions at the vascular wall
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-8957275 Post-translational protein phosphorylation

Miscellaneous databases

ChiTaRSiProc mouse
PROiPR:P33587
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024386 Expressed in 85 organ(s), highest expression level in liver
CleanExiMM_PROC
GenevisibleiP33587 MM

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
Gene3Di4.10.740.10, 1 hit
InterProiView protein in InterPro
IPR017857 Coagulation_fac-like_Gla_dom
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR035972 GLA-like_dom_SF
IPR000294 GLA_domain
IPR012224 Pept_S1A_FX
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00008 EGF, 1 hit
PF00594 Gla, 1 hit
PF00089 Trypsin, 1 hit
PIRSFiPIRSF001143 Factor_X, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
PR00001 GLABLOOD
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00179 EGF_CA, 2 hits
SM00069 GLA, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57630 SSF57630, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 2 hits
PS50026 EGF_3, 1 hit
PS01187 EGF_CA, 1 hit
PS00011 GLA_1, 1 hit
PS50998 GLA_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPROC_MOUSE
AccessioniPrimary (citable) accession number: P33587
Secondary accession number(s): O35498, Q91WN8, Q99PC6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 7, 2004
Last modified: November 7, 2018
This is version 182 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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