UniProtKB - P33570 (TKT2_ECOLI)
Protein
Transketolase 2
Gene
tktB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate. Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate (By similarity).By similarity
Catalytic activityi
- D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphateEC:2.2.1.1
Cofactori
Protein has several cofactor binding sites:- Mg2+By similarity, Ca2+By similarity, Mn2+By similarity, Co2+By similarityNote: Binds 1 Mg2+ ion per subunit. Can also utilize other divalent metal cations, such as Ca2+, Mn2+ and Co2+.By similarity
- thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 25 | SubstrateBy similarity | 1 | |
Sitei | 25 | Important for catalytic activityBy similarity | 1 | |
Binding sitei | 65 | Thiamine pyrophosphateBy similarity | 1 | |
Metal bindingi | 154 | MagnesiumBy similarity | 1 | |
Binding sitei | 155 | Thiamine pyrophosphate; via amide nitrogenBy similarity | 1 | |
Metal bindingi | 184 | MagnesiumBy similarity | 1 | |
Binding sitei | 184 | Thiamine pyrophosphateBy similarity | 1 | |
Metal bindingi | 186 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 260 | SubstrateBy similarity | 1 | |
Binding sitei | 260 | Thiamine pyrophosphateBy similarity | 1 | |
Sitei | 260 | Important for catalytic activityBy similarity | 1 | |
Binding sitei | 357 | SubstrateBy similarity | 1 | |
Binding sitei | 384 | SubstrateBy similarity | 1 | |
Active sitei | 410 | Proton donorBy similarity | 1 | |
Binding sitei | 436 | Thiamine pyrophosphateBy similarity | 1 | |
Binding sitei | 460 | SubstrateBy similarity | 1 | |
Binding sitei | 468 | SubstrateBy similarity | 1 | |
Binding sitei | 519 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 113 – 115 | Thiamine pyrophosphateBy similarity | 3 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- transketolase activity Source: EcoliWiki
GO - Biological processi
- pentose-phosphate shunt Source: GO_Central
Keywordsi
Molecular function | Transferase |
Ligand | Calcium, Magnesium, Metal-binding, Thiamine pyrophosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:TRANSKETOII-MONOMER MetaCyc:TRANSKETOII-MONOMER |
BRENDAi | 2.2.1.1, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:tktB Ordered Locus Names:b2465, JW2449 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191857 | 1 – 667 | Transketolase 2Add BLAST | 667 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 342 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P33570 |
PaxDbi | P33570 |
PRIDEi | P33570 |
PTM databases
iPTMneti | P33570 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 4262216, 19 interactors |
DIPi | DIP-10999N |
IntActi | P33570, 6 interactors |
STRINGi | 511145.b2465 |
Family & Domainsi
Sequence similaritiesi
Belongs to the transketolase family.Curated
Phylogenomic databases
eggNOGi | COG0021, Bacteria |
HOGENOMi | CLU_009227_0_0_6 |
InParanoidi | P33570 |
PhylomeDBi | P33570 |
Family and domain databases
CDDi | cd02012, TPP_TK, 1 hit |
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR005478, Transketolase_bac-like IPR020826, Transketolase_BS IPR033248, Transketolase_C IPR033247, Transketolase_fam IPR005474, Transketolase_N |
PANTHERi | PTHR43522, PTHR43522, 1 hit |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit PF00456, Transketolase_N, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
TIGRFAMsi | TIGR00232, tktlase_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00801, TRANSKETOLASE_1, 1 hit PS00802, TRANSKETOLASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P33570-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRKDLANAI RALSMDAVQK ANSGHPGAPM GMADIAEVLW NDFLKHNPTD
60 70 80 90 100
PTWYDRDRFI LSNGHASMLL YSLLHLTGYD LPLEELKNFR QLHSKTPGHP
110 120 130 140 150
EIGYTPGVET TTGPLGQGLA NAVGLAIAER TLAAQFNQPD HEIVDHFTYV
160 170 180 190 200
FMGDGCLMEG ISHEVCSLAG TLGLGKLIGF YDHNGISIDG ETEGWFTDDT
210 220 230 240 250
AKRFEAYHWH VIHEIDGHDP QAVKEAILEA QSVKDKPSLI ICRTVIGFGS
260 270 280 290 300
PNKAGKEEAH GAPLGEEEVA LARQKLGWHH PPFEIPKEIY HAWDAREKGE
310 320 330 340 350
KAQQSWNEKF AAYKKAHPQL AEEFTRRMSG GLPKDWEKTT QKYINELQAN
360 370 380 390 400
PAKIATRKAS QNTLNAYGPM LPELLGGSAD LAPSNLTIWK GSVSLKEDPA
410 420 430 440 450
GNYIHYGVRE FGMTAIANGI AHHGGFVPYT ATFLMFVEYA RNAARMAALM
460 470 480 490 500
KARQIMVYTH DSIGLGEDGP THQAVEQLAS LRLTPNFSTW RPCDQVEAAV
510 520 530 540 550
GWKLAVERHN GPTALILSRQ NLAQVERTPD QVKEIARGGY VLKDSGGKPD
560 570 580 590 600
IILIATGSEM EITLQAAEKL AGEGRNVRVV SLPSTDIFDA QDEEYRESVL
610 620 630 640 650
PSNVAARVAV EAGIADYWYK YVGLKGAIVG MTGYGESAPA DKLFPFFGFT
660
AENIVAKAHK VLGVKGA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D12473 Genomic DNA Translation: BAA02039.1 U00096 Genomic DNA Translation: AAC75518.1 AP009048 Genomic DNA Translation: BAA16340.1 |
PIRi | A48660 |
RefSeqi | NP_416960.1, NC_000913.3 WP_000087280.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75518; AAC75518; b2465 BAA16340; BAA16340; BAA16340 |
GeneIDi | 57729461 945865 |
KEGGi | ecj:JW2449 eco:b2465 |
PATRICi | fig|511145.12.peg.2559 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D12473 Genomic DNA Translation: BAA02039.1 U00096 Genomic DNA Translation: AAC75518.1 AP009048 Genomic DNA Translation: BAA16340.1 |
PIRi | A48660 |
RefSeqi | NP_416960.1, NC_000913.3 WP_000087280.1, NZ_LN832404.1 |
3D structure databases
SMRi | P33570 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262216, 19 interactors |
DIPi | DIP-10999N |
IntActi | P33570, 6 interactors |
STRINGi | 511145.b2465 |
PTM databases
iPTMneti | P33570 |
Proteomic databases
jPOSTi | P33570 |
PaxDbi | P33570 |
PRIDEi | P33570 |
Genome annotation databases
EnsemblBacteriai | AAC75518; AAC75518; b2465 BAA16340; BAA16340; BAA16340 |
GeneIDi | 57729461 945865 |
KEGGi | ecj:JW2449 eco:b2465 |
PATRICi | fig|511145.12.peg.2559 |
Organism-specific databases
EchoBASEi | EB2024 |
Phylogenomic databases
eggNOGi | COG0021, Bacteria |
HOGENOMi | CLU_009227_0_0_6 |
InParanoidi | P33570 |
PhylomeDBi | P33570 |
Enzyme and pathway databases
BioCyci | EcoCyc:TRANSKETOII-MONOMER MetaCyc:TRANSKETOII-MONOMER |
BRENDAi | 2.2.1.1, 2026 |
Miscellaneous databases
PROi | PR:P33570 |
Family and domain databases
CDDi | cd02012, TPP_TK, 1 hit |
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR005478, Transketolase_bac-like IPR020826, Transketolase_BS IPR033248, Transketolase_C IPR033247, Transketolase_fam IPR005474, Transketolase_N |
PANTHERi | PTHR43522, PTHR43522, 1 hit |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit PF00456, Transketolase_N, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
TIGRFAMsi | TIGR00232, tktlase_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00801, TRANSKETOLASE_1, 1 hit PS00802, TRANSKETOLASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TKT2_ECOLI | |
Accessioni | P33570Primary (citable) accession number: P33570 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families