UniProtKB - P33563 (MTBB_BACIU)
Protein
Modification methylase BsuBI
Gene
hsdBM
Organism
Bacillus subtilis
Status
Functioni
This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strand, and protects the DNA from cleavage by the BsuBI endonuclease.
Catalytic activityi
- a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H+ + S-adenosyl-L-homocysteineEC:2.1.1.72
GO - Molecular functioni
- DNA binding Source: InterPro
- site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
GO - Biological processi
- DNA restriction-modification system Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Restriction system |
Ligand | S-adenosyl-L-methionine |
Protein family/group databases
REBASEi | 203431, M.Bat1359ORF636P 3336, M.BsuBI |
Names & Taxonomyi
Protein namesi | Recommended name: Modification methylase BsuBI (EC:2.1.1.72)Short name: M.BsuBI Alternative name(s): Adenine-specific methyltransferase BsuBI |
Gene namesi | Name:hsdBM Synonyms:hsmB |
Organismi | Bacillus subtilis |
Taxonomic identifieri | 1423 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087946 | 1 – 501 | Modification methylase BsuBIAdd BLAST | 501 |
Family & Domainsi
Sequence similaritiesi
Belongs to the N(4)/N(6)-methyltransferase family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR011639, RM_methylase_Eco57I IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF07669, Eco57I, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P33563-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTQILETVDK SRLTVNPLLK NKSELGQFFT PSSISIFMAC LFSEDKLNNA
60 70 80 90 100
KVLDAGAGIG SLTSAFLARL ISENIGKADL HLLEIDEMLE PYLSETLALF
110 120 130 140 150
KDYIEINSQI IIDDFIEWAA YSLLDEESLL AKDKQRFTHA ILNPPYKKIK
160 170 180 190 200
SNSKHRKLLR KAGIETVNLY SAFVALTVDL MSDGGEIVFI IPRSFCNGPY
210 220 230 240 250
FRHFRQHLLN KTSIKHMHLF ESRDKAFKDD EVLQENVISK LEKGTVQEDV
260 270 280 290 300
KISISTDDSF SVIRSYRYPF EKIVQPNDIE KFIHINTTNE ETLIEKHPNV
310 320 330 340 350
CYSLEELNIE VSTGPVVDFR VKENLREMPG EGTVPLFYPN HFVGTSLEYP
360 370 380 390 400
KMMKKPNAII RNEKVEKWLY PNGHYVVVKR FSSKEEKRRI VAGVLTPESV
410 420 430 440 450
NDPVVGFENG LNVLHYNKSG ISKEVAYGLY AYLNSTPVDK YFRIFNGHTQ
460 470 480 490 500
VNATDLRTMK FPSRDILISL GKWVIENIEN VGQVEIDSKL EELLLNDRGN
A
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L01541 Genomic DNA Translation: AAA18169.1 |
PIRi | S35515 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L01541 Genomic DNA Translation: AAA18169.1 |
PIRi | S35515 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein family/group databases
REBASEi | 203431, M.Bat1359ORF636P 3336, M.BsuBI |
Miscellaneous databases
PROi | PR:P33563 |
Family and domain databases
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR011639, RM_methylase_Eco57I IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF07669, Eco57I, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MTBB_BACIU | |
Accessioni | P33563Primary (citable) accession number: P33563 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | December 11, 2019 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- SIMILARITY comments
Index of protein domains and families - Restriction enzymes and methylases
Classification of restriction enzymes and methylases and list of entries