UniProtKB - P33533 (OPRD_RAT)
Protein
Delta-type opioid receptor
Gene
Oprd1
Organism
Rattus norvegicus (Rat)
Status
Functioni
G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.2 Publications
GO - Molecular functioni
- enkephalin receptor activity Source: RGD
- G protein-coupled receptor activity Source: GO_Central
- neuropeptide binding Source: GO_Central
- opioid receptor activity Source: RGD
- peptide binding Source: GO_Central
- receptor serine/threonine kinase binding Source: RGD
GO - Biological processi
- adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: RGD
- adult locomotory behavior Source: RGD
- cellular response to growth factor stimulus Source: RGD
- cellular response to hypoxia Source: RGD
- cellular response to toxic substance Source: RGD
- eating behavior Source: RGD
- G protein-coupled receptor signaling pathway Source: RGD
- negative regulation of gene expression Source: RGD
- negative regulation of protein-containing complex assembly Source: RGD
- neuropeptide signaling pathway Source: RGD
- opioid receptor signaling pathway Source: RGD
- phospholipase C-activating G protein-coupled receptor signaling pathway Source: UniProtKB
- positive regulation of peptidyl-serine phosphorylation Source: RGD
- regulation of calcium ion transport Source: RGD
- regulation of mitochondrial membrane potential Source: RGD
- regulation of sensory perception of pain Source: RGD
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-RNO-375276, Peptide ligand-binding receptors R-RNO-418594, G alpha (i) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: Delta-type opioid receptorShort name: D-OR-1 Short name: DOR-1 Alternative name(s): Opioid receptor A |
Gene namesi | Name:Oprd1 Synonyms:Ror-a |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3233, Oprd1 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein 2 Publications
Plasma Membrane
- dendrite membrane Source: RGD
- integral component of plasma membrane Source: GO_Central
- integral component of postsynaptic density membrane Source: SynGO
- integral component of presynaptic membrane Source: SynGO
- intrinsic component of plasma membrane Source: RGD
- plasma membrane Source: RGD
- postsynaptic membrane Source: RGD
Other locations
- axon terminus Source: RGD
- cytoplasm Source: RGD
- integral component of membrane Source: RGD
- integral component of synaptic vesicle membrane Source: SynGO
- membrane Source: RGD
- membrane raft Source: RGD
- neuron projection Source: GO_Central
- neuronal dense core vesicle Source: SynGO
- spine apparatus Source: SynGO
- vesicle Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 47 | ExtracellularBy similarityAdd BLAST | 47 | |
Transmembranei | 48 – 75 | Helical; Name=1By similarityAdd BLAST | 28 | |
Topological domaini | 76 – 85 | CytoplasmicBy similarity | 10 | |
Transmembranei | 86 – 110 | Helical; Name=2By similarityAdd BLAST | 25 | |
Topological domaini | 111 – 122 | ExtracellularBy similarityAdd BLAST | 12 | |
Transmembranei | 123 – 144 | Helical; Name=3By similarityAdd BLAST | 22 | |
Topological domaini | 145 – 163 | CytoplasmicBy similarityAdd BLAST | 19 | |
Transmembranei | 164 – 186 | Helical; Name=4By similarityAdd BLAST | 23 | |
Topological domaini | 187 – 206 | ExtracellularBy similarityAdd BLAST | 20 | |
Transmembranei | 207 – 238 | Helical; Name=5By similarityAdd BLAST | 32 | |
Topological domaini | 239 – 261 | CytoplasmicBy similarityAdd BLAST | 23 | |
Transmembranei | 262 – 284 | Helical; Name=6By similarityAdd BLAST | 23 | |
Topological domaini | 285 – 299 | ExtracellularBy similarityAdd BLAST | 15 | |
Transmembranei | 300 – 321 | Helical; Name=7By similarityAdd BLAST | 22 | |
Topological domaini | 322 – 372 | CytoplasmicBy similarityAdd BLAST | 51 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069965 | 1 – 372 | Delta-type opioid receptorAdd BLAST | 372 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 18 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 33 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 121 ↔ 198 | PROSITE-ProRule annotation | ||
Lipidationi | 333 | S-palmitoyl cysteineSequence analysis | 1 |
Post-translational modificationi
Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Ubl conjugationProteomic databases
PaxDbi | P33533 |
PRIDEi | P33533 |
PTM databases
GlyGeni | P33533, 2 sites |
iPTMneti | P33533 |
PhosphoSitePlusi | P33533 |
Expressioni
Tissue specificityi
Detected in brain, brain stem and brain cortex.2 Publications
Gene expression databases
Bgeei | ENSRNOG00000010531, Expressed in frontal cortex and 12 other tissues |
Genevisiblei | P33533, RN |
Interactioni
Subunit structurei
May form homooligomers.
Forms a heterodimer with OPRM1.
Interacts with GPRASP1.
Interacts with RTP4; the interaction promotes cell surface localization of the OPRD1-OPRM1 heterodimer.
By similarityGO - Molecular functioni
- receptor serine/threonine kinase binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000014084 |
Chemistry databases
BindingDBi | P33533 |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00940000157669 |
HOGENOMi | CLU_009579_8_1_1 |
InParanoidi | P33533 |
OMAi | MYGVIRY |
OrthoDBi | 1011272at2759 |
PhylomeDBi | P33533 |
TreeFami | TF315737 |
Family and domain databases
InterProi | View protein in InterPro IPR000321, Delta_opi_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001418, Opioid_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00525, DELTAOPIOIDR PR00237, GPCRRHODOPSN PR00384, OPIOIDR |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P33533-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEPVPSARAE LQFSLLANVS DTFPSAFPSA SANASGSPGA RSASSLALAI
60 70 80 90 100
AITALYSAVC AVGLLGNVLV MFGIVRYTKL KTATNIYIFN LALADALATS
110 120 130 140 150
TLPFQSAKYL METWPFGELL CKAVLSIDYY NMFTSIFTLT MMSVDRYIAV
160 170 180 190 200
CHPVKALDFR TPAKAKLINI CIWVLASGVG VPIMVMAVTQ PRDGAVVCTL
210 220 230 240 250
QFPSPSWYWD TVTKICVFLF AFVVPILIIT VCYGLMLLRL RSVRLLSGSK
260 270 280 290 300
EKDRSLRRIT RMVLVVVGAF VVCWAPIHIF VIVWTLVDIN RRDPLVVAAL
310 320 330 340 350
HLCIALGYAN SSLNPVLYAF LDENFKRCFR QLCRAPCGGQ EPGSLRRPRQ
360 370
ATARERVTAC TPSDGPGGGA AA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D16348 mRNA Translation: BAA03851.1 U00475 mRNA Translation: AAA19939.1 |
PIRi | S34592 |
RefSeqi | NP_036749.1, NM_012617.1 |
Genome annotation databases
Ensembli | ENSRNOT00000014084; ENSRNOP00000014084; ENSRNOG00000010531 |
GeneIDi | 24613 |
KEGGi | rno:24613 |
UCSCi | RGD:3233, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D16348 mRNA Translation: BAA03851.1 U00475 mRNA Translation: AAA19939.1 |
PIRi | S34592 |
RefSeqi | NP_036749.1, NM_012617.1 |
3D structure databases
SMRi | P33533 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000014084 |
Chemistry databases
BindingDBi | P33533 |
ChEMBLi | CHEMBL269 |
DrugCentrali | P33533 |
GuidetoPHARMACOLOGYi | 317 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P33533, 2 sites |
iPTMneti | P33533 |
PhosphoSitePlusi | P33533 |
Proteomic databases
PaxDbi | P33533 |
PRIDEi | P33533 |
Genome annotation databases
Ensembli | ENSRNOT00000014084; ENSRNOP00000014084; ENSRNOG00000010531 |
GeneIDi | 24613 |
KEGGi | rno:24613 |
UCSCi | RGD:3233, rat |
Organism-specific databases
CTDi | 4985 |
RGDi | 3233, Oprd1 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00940000157669 |
HOGENOMi | CLU_009579_8_1_1 |
InParanoidi | P33533 |
OMAi | MYGVIRY |
OrthoDBi | 1011272at2759 |
PhylomeDBi | P33533 |
TreeFami | TF315737 |
Enzyme and pathway databases
Reactomei | R-RNO-375276, Peptide ligand-binding receptors R-RNO-418594, G alpha (i) signalling events |
Miscellaneous databases
PROi | PR:P33533 |
Gene expression databases
Bgeei | ENSRNOG00000010531, Expressed in frontal cortex and 12 other tissues |
Genevisiblei | P33533, RN |
Family and domain databases
InterProi | View protein in InterPro IPR000321, Delta_opi_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM IPR001418, Opioid_rcpt |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR00525, DELTAOPIOIDR PR00237, GPCRRHODOPSN PR00384, OPIOIDR |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OPRD_RAT | |
Accessioni | P33533Primary (citable) accession number: P33533 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries - SIMILARITY comments
Index of protein domains and families