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Protein

THO complex subunit MFT1

Gene

MFT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component the THO subcomplex of the TREX complex, which operates in coupling transcription elongation to mRNA export. The THO complex is recruited to transcribed genes and moves along the gene with the elongating polymerase during transcription. THO is important for stabilizing nascent RNA in the RNA polymerase II elongation complex by preventing formation of DNA:RNA hybrids behind the elongating polymerase. It functions in cotranscriptional formation of an export-competent messenger ribonucleoprotein particle (mRNP) by facilitating the loading of ATP-dependent RNA helicase SUB2 and the mRNA export factor YRA1 along the nascent mRNA.4 Publications

Miscellaneous

Present with 5910 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be involved in mitochondrial protein import.1 Publication

GO - Molecular functioni

  • nucleic acid binding Source: SGD
  • protein-containing complex scaffold activity Source: SGD

GO - Biological processi

  • DNA recombination Source: SGD
  • mRNA export from nucleus Source: SGD
  • mRNA processing Source: InterPro
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • stress granule assembly Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32657-MONOMER

Protein family/group databases

TCDBi3.A.22.1.1 the transcription-coupled trex/tap nuclear mrna export complex (trex) family

Names & Taxonomyi

Protein namesi
Recommended name:
THO complex subunit MFT1
Alternative name(s):
Mitochondrial fusion target protein 1
Gene namesi
Name:MFT1
Ordered Locus Names:YML062C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML062C
SGDiS000004527 MFT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000964631 – 392THO complex subunit MFT1Add BLAST392

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei266PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP33441
PaxDbiP33441
PRIDEiP33441

PTM databases

iPTMnetiP33441

Interactioni

Subunit structurei

Component of the THO complex, which is composed of HPR1, MFT1, THO2 and THP2. Together with SUB2, TEX1 and YRA1, THO forms the transcription/export (TREX) complex. THO associates with DNA and RNA in vitro.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein-containing complex scaffold activity Source: SGD

Protein-protein interaction databases

BioGridi35104, 237 interactors
ComplexPortaliCPX-1792 THO complex
CPX-1793 TREX complex
DIPiDIP-4537N
IntActiP33441, 61 interactors
MINTiP33441
STRINGi4932.YML062C

Structurei

3D structure databases

ProteinModelPortaliP33441
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

InParanoidiP33441
KOiK18714
OMAiQPANDED
OrthoDBiEOG092C35E1

Family and domain databases

InterProiView protein in InterPro
IPR008501 THOC7/Mft1
PfamiView protein in Pfam
PF05615 THOC7, 1 hit

Sequencei

Sequence statusi: Complete.

P33441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLSQKQIDQ VRTKVHYSEV DTPFNKYLDI LGKVTKLTGS IINGTLSNDD
60 70 80 90 100
SKIEKLTEQN ISQLKESAHL RFLDLQSSID TKKVADENWE TCQQETLAKL
110 120 130 140 150
ENLKDKLPDI KSIHSKLLLR IGKLQGLYDS VQVINREVEG LSEGRTSLVV
160 170 180 190 200
TRAEWEKELG TDLVKFLIEK NYLKLVDPGL KKDSSEERYR IYDDFSKGPK
210 220 230 240 250
ELESINASMK SDIENVRQEV SSYKEKWLRD AEIFGKITSI FKEELLKRDG
260 270 280 290 300
LLNEAEGDNI DEDYESDEDE ERKERFKRQR SMVEVNTIEN VDEKEESDHE
310 320 330 340 350
YDDQEDEENE EEDDMEVDVE DIKEDNEVDG ESSQQEDNSR QGNNEETDKE
360 370 380 390
TGVIEEPDAV NDAEEADSDH SSRKLGGTTS DFSASSSVEE VK
Length:392
Mass (Da):44,996
Last modified:February 1, 1994 - v1
Checksum:i45A775F8C1DA3E29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S57517 Genomic DNA Translation: AAB26005.1
Z38114 Genomic DNA Translation: CAA86259.1
BK006946 Genomic DNA Translation: DAA09835.1
PIRiS32405
RefSeqiNP_013649.1, NM_001182421.1

Genome annotation databases

EnsemblFungiiYML062C; YML062C; YML062C
GeneIDi854940
KEGGisce:YML062C

Similar proteinsi

Entry informationi

Entry nameiMFT1_YEAST
AccessioniPrimary (citable) accession number: P33441
Secondary accession number(s): D6VZB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 18, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

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