UniProtKB - P33426 (CAPSD_HEVPA)
Secreted protein ORF2
ORF2
Functioni
Plays a role in the inhibition of host antibody-mediated neutralization without blocking viral cell entry.
By similarityForms an icosahedral capsid with a T=1 symmetry and a 34 nm diameter. The capsid is composed of 60 copies linked to each other. Binds to the 5' end of the genomic RNA to mediate genome encapsidation (By similarity).
Binds to heparin surface proteoglycans (HSPGs) to mediate viral entry. Additionally, the interactions with host ASGR1 and ASGR2 facilitate viral infection of hepatocytes (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: IntAct
- RNA binding Source: UniProtKB-KW
- structural molecule activity Source: InterPro
GO - Biological processi
- entry receptor-mediated virion attachment to host cell Source: UniProtKB-KW
- viral entry into host cell Source: UniProtKB-KW
Keywordsi
Molecular function | RNA-binding |
Biological process | Host-virus interaction, Viral attachment to host cell, Viral attachment to host entry receptor, Virus entry into host cell |
Names & Taxonomyi
Protein namesi | Recommended name: Secreted protein ORF2Alternative name(s): Protein ORF2 Short name: pORF2 |
Gene namesi | ORF Names:ORF2 |
Organismi | Hepatitis E virus genotype 1 (isolate Human/Pakistan/Sar-55) (HEV-1) |
Taxonomic identifieri | 33774 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Kitrinoviricota › Alsuviricetes › Hepelivirales › Hepeviridae › Orthohepevirus › Hepatitis E virus |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Secreted By similarity
- Virion By similarity
- Host cytoplasm By similarity
- Host endoplasmic reticulum By similarity
- Host Golgi apparatus By similarity
- Host cell surface By similarity
Keywords - Cellular componenti
Capsid protein, Host cytoplasm, Host endoplasmic reticulum, Host Golgi apparatus, Secreted, T=1 icosahedral capsid protein, VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 137 | N → Q: Complete loss of infectivity. Inhibition of capsid assembly. 1 Publication | 1 | |
Mutagenesisi | 310 | N → Q: Complete loss of infectivity. Inhibition of capsid assembly. 1 Publication | 1 | |
Mutagenesisi | 445 | N → Q: Complete loss of infectivity. 1 Publication | 1 | |
Mutagenesisi | 562 | N → Q: Complete loss of infectivity. Loss of dimerization. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000445489 | 1 – 660 | Secreted protein ORF2Add BLAST | 660 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 137 | N-linked (GlcNAc...) asparagine; by hostBy similarity | 1 | |
Glycosylationi | 310 | N-linked (GlcNAc...) asparagine; by hostBy similarity | 1 | |
Glycosylationi | 562 | N-linked (GlcNAc...) asparagine; by hostBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
GlycoproteinInteractioni
Subunit structurei
Self-assembles to form the capsid. The capsid is dominated by dimers that define the 30 morphological units.
Interacts with phosphorylated protein ORF3 (By similarity).
Interacts with host TMEM134.
Interacts with host ASGR1 and ASGR2; these interactions facilitate infection of host hepatocytes (By similarity).
By similarityBinary interactionsi
P33426
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-15929932,EBI-15929932 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
DIPi | DIP-59683N |
Structurei
Secondary structure
3D structure databases
SMRi | P33426 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P33426 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 18 – 44 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 66 – 127 | DisorderedSequence analysisAdd BLAST | 62 | |
Regioni | 368 – 394 | particle formationBy similarityAdd BLAST | 27 | |
Regioni | 585 – 610 | OligomerizationBy similarityAdd BLAST | 26 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 2.60.120.20, 1 hit |
InterProi | View protein in InterPro IPR004261, SP2 IPR029053, Viral_coat |
Pfami | View protein in Pfam PF03014, SP2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRPRPILLLL LMFLPMLPAP PPGQPSGRRR GRRSGGSGGG FWGDRVDSQP
60 70 80 90 100
FAIPYIHPTN PFAPDVTAAA GAGPRVRQPA RPLGSAWRDQ AQRPAAASRR
110 120 130 140 150
RPTTAGAAPL TAVAPAHDTP PVPDVDSRGA ILRRQYNLST SPLTSSVATG
160 170 180 190 200
TNLVLYAAPL SPLLPLQDGT NTHIMATEAS NYAQYRVARA TIRYRPLVPN
210 220 230 240 250
AVGGYAISIS FWPQTTTTPT SVDMNSITST DVRILVQPGI ASELVIPSER
260 270 280 290 300
LHYRNQGWRS VETSGVAEEE ATSGLVMLCI HGSPVNSYTN TPYTGALGLL
310 320 330 340 350
DFALELEFRN LTPGNTNTRV SRYSSTARHR LRRGADGTAE LTTTAATRFM
360 370 380 390 400
KDLYFTSTNG VGEIGRGIAL TLFNLADTLL GGLPTELISS AGGQLFYSRP
410 420 430 440 450
VVSANGEPTV KLYTSVENAQ QDKGIAIPHD IDLGESRVVI QDYDNQHEQD
460 470 480 490 500
RPTPSPAPSR PFSVLRANDV LWLSLTAAEY DQSTYGSSTG PVYVSDSVTL
510 520 530 540 550
VNVATGAQAV ARSLDWTKVT LDGRPLSTIQ QYSKTFFVLP LRGKLSFWEA
560 570 580 590 600
GTTKAGYPYN YNTTASDQLL VENAAGHRVA ISTYTTSLGA GPVSISAVAV
610 620 630 640 650
LAPHSVLALL EDTMDYPARA HTFDDFCPEC RPLGLQGCAF QSTVAELQRL
660
KMKVGKTREL
The sequence of this isoform differs from the canonical sequence as follows:
1-15: Missing.
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_059889 | 1 – 15 | Missing in isoform Capsid protein ORF2. Add BLAST | 15 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M80581 Genomic RNA Translation: AAA45727.1 AF444002 Genomic RNA Translation: AAL50056.1 AF444003 Genomic RNA Translation: AAL50059.1 |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M80581 Genomic RNA Translation: AAA45727.1 AF444002 Genomic RNA Translation: AAL50056.1 AF444003 Genomic RNA Translation: AAL50059.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3GGQ | X-ray | 2.00 | A | 455-602 | [»] | |
3RKD | X-ray | 1.90 | A/B | 459-603 | [»] | |
6LAT | electron microscopy | 3.40 | A | 129-605 | [»] | |
6LB0 | electron microscopy | 3.60 | A | 129-605 | [»] | |
SMRi | P33426 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-59683N |
Protocols and materials databases
ABCDi | P33426, 1 sequenced antibody |
Miscellaneous databases
EvolutionaryTracei | P33426 |
Family and domain databases
Gene3Di | 2.60.120.20, 1 hit |
InterProi | View protein in InterPro IPR004261, SP2 IPR029053, Viral_coat |
Pfami | View protein in Pfam PF03014, SP2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CAPSD_HEVPA | |
Accessioni | P33426Primary (citable) accession number: P33426 Secondary accession number(s): Q77EC8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 84 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families