UniProtKB - P33418 (XPOT_YEAST)
Exportin-T
LOS1
Functioni
tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex. Preferentially interacts with tRNAs with mature 5'- and 3'-termini and does not distinguish between intron-containing and spliced tRNAs. In the nucleus binds to tRNA and to the Ran-GTPases GSP1 or GSP2 in their active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP cause release of the tRNA from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
13 PublicationsMiscellaneous
Caution
GO - Molecular functioni
- small GTPase binding Source: SGD
- tRNA binding Source: GO_Central
GO - Biological processi
- tRNA export from nucleus Source: SGD
- tRNA re-export from nucleus Source: SGD
Keywordsi
Molecular function | RNA-binding, tRNA-binding |
Biological process | Transport |
Protein family/group databases
TCDBi | 9.A.50.1.1, the nuclear t-rna exporter (trna-e) family |
Names & Taxonomyi
Protein namesi | Recommended name: Exportin-TAlternative name(s): Exportin(tRNA) Karyopherin-beta tRNA exportin |
Gene namesi | Name:LOS1 Ordered Locus Names:YKL205W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001688, LOS1 |
VEuPathDBi | FungiDB:YKL205W |
Subcellular locationi
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nuclear matrix Source: SGD
- nuclear pore Source: GO_Central
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084460 | 1 – 1100 | Exportin-TAdd BLAST | 1100 |
Proteomic databases
MaxQBi | P33418 |
PaxDbi | P33418 |
PRIDEi | P33418 |
PTM databases
iPTMneti | P33418 |
Interactioni
Subunit structurei
Interacts with CEX1, GSP1, GSP2, NSP1, NUP2 and UTP8.
3 PublicationsBinary interactionsi
P33418
With | #Exp. | IntAct |
---|---|---|
CEX1 [Q12453] | 2 | EBI-10188,EBI-31271 |
GO - Molecular functioni
- small GTPase binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 33918, 174 interactors |
DIPi | DIP-760N |
IntActi | P33418, 8 interactors |
MINTi | P33418 |
STRINGi | 4932.YKL205W |
Miscellaneous databases
RNActi | P33418, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2021, Eukaryota |
GeneTreei | ENSGT00390000007890 |
HOGENOMi | CLU_004414_0_1_1 |
InParanoidi | P33418 |
OMAi | RFSPLCW |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR013598, Exportin-1/Importin-b-like IPR045546, Exportin-T_C IPR040017, XPOT |
PANTHERi | PTHR15952, PTHR15952, 1 hit |
Pfami | View protein in Pfam PF19282, Exportin-T, 2 hits PF08389, Xpo1, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MLERIQQLVN AVNDPRSDVA TKRQAIELLN GIKSSENALE IFISLVINEN
60 70 80 90 100
SNDLLKFYGL STLIELMTEG VNANPNGLNL VKFEITKWLK FQVLGNKQTK
110 120 130 140 150
LPDFLMNKIS EVLTTLFMLM YSDCNGNQWN SFFDDLMSLF QVDSAISNTS
160 170 180 190 200
PSTDGNILLG LEFFNKLCLM INSEIADQSF IRSKESQLKN NNIKDWMRDN
210 220 230 240 250
DIMKLSNVWF QCLKLDEQIV SQCPGLINST LDCIGSFISW IDINLIIDAN
260 270 280 290 300
NYYLQLIYKF LNLKETKISC YNCILAIISK KMKPMDKLAF LNMINLTNEL
310 320 330 340 350
TYYHQAISMN PQIITFDNLE VWESLTKLIT SFGIEFTIII EQVNDDQKLD
360 370 380 390 400
TLYKQSVISN VDSILLEKII PILLEFMNNE FDSITAKTFP FWSNYLAFLK
410 420 430 440 450
KYKASSPNFV PLHKDFLDNF QQICFKRMKF SDDEVTQDDF EEFNETVRFK
460 470 480 490 500
LKNFQEIIVV IDPSLFLNNI SQEISANLMN CKNESWQIFE LTIYQIFNLS
510 520 530 540 550
ECTKNNYFGL NKNEIMTSQP SLTLVRFLNE LLMMKDFLLA IDNEQIQILF
560 570 580 590 600
MELIVKNYNF IFSTSANTAN ATDDDEKYLL ILNIFMSSFA MFNKRENVRL
610 620 630 640 650
RSWYLFTRFL KLTRINLKKI LFANKNLVNE ITNKISPLLH IKVTSINAQG
660 670 680 690 700
TDDNDTIFDN QLYIFEGIGF IITLNNSSQE LTAATANTPI DYDILDQILT
710 720 730 740 750
PLFTQLEGCI TQGASPVVIL ECHHILMAIG TLARGLHIGL VPENQVNNMV
760 770 780 790 800
VNKKLINDSL IHKFSNIAEV ILVTFSFFNK FENIRDASRF TFARLIPILS
810 820 830 840 850
NKILPFINKL IELILSSTDL KSWEMIDFLG FLSQLIHMFH TDTDCYQLFN
860 870 880 890 900
QLLTPLINKV HSIIEEIDEQ HDQQSSSNKP IDTAVTATSV NKNIVVTDSY
910 920 930 940 950
RDKILLKKAY CTFLQSFTNN SVTSILLSDI NRAILPVILN DLVTYTPQEI
960 970 980 990 1000
QETSMMKVSL NVLCNFIKCF GNGTCLDNDD INKDPNLKID GLNEYFIMKC
1010 1020 1030 1040 1050
VPIIFEIPFN PIYKFNIKEG NFKTMAYDLA RLLRELFIVS SNPTTNENEC
1060 1070 1080 1090 1100
VKYLTQIYLP QIQLPQELTI QLVNMLTTMG QKQFEKWFVD NFISVLKQGQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13941 Unassigned DNA Translation: AAC37342.1 Z28205 Genomic DNA Translation: CAA82050.1 BK006944 Genomic DNA Translation: DAA08964.1 |
PIRi | S38043 |
RefSeqi | NP_012717.1, NM_001179770.1 |
Genome annotation databases
EnsemblFungii | YKL205W_mRNA; YKL205W; YKL205W |
GeneIDi | 853630 |
KEGGi | sce:YKL205W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13941 Unassigned DNA Translation: AAC37342.1 Z28205 Genomic DNA Translation: CAA82050.1 BK006944 Genomic DNA Translation: DAA08964.1 |
PIRi | S38043 |
RefSeqi | NP_012717.1, NM_001179770.1 |
3D structure databases
AlphaFoldDBi | P33418 |
SMRi | P33418 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33918, 174 interactors |
DIPi | DIP-760N |
IntActi | P33418, 8 interactors |
MINTi | P33418 |
STRINGi | 4932.YKL205W |
Protein family/group databases
TCDBi | 9.A.50.1.1, the nuclear t-rna exporter (trna-e) family |
PTM databases
iPTMneti | P33418 |
Proteomic databases
MaxQBi | P33418 |
PaxDbi | P33418 |
PRIDEi | P33418 |
Genome annotation databases
EnsemblFungii | YKL205W_mRNA; YKL205W; YKL205W |
GeneIDi | 853630 |
KEGGi | sce:YKL205W |
Organism-specific databases
SGDi | S000001688, LOS1 |
VEuPathDBi | FungiDB:YKL205W |
Phylogenomic databases
eggNOGi | KOG2021, Eukaryota |
GeneTreei | ENSGT00390000007890 |
HOGENOMi | CLU_004414_0_1_1 |
InParanoidi | P33418 |
OMAi | RFSPLCW |
Miscellaneous databases
PROi | PR:P33418 |
RNActi | P33418, protein |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR013598, Exportin-1/Importin-b-like IPR045546, Exportin-T_C IPR040017, XPOT |
PANTHERi | PTHR15952, PTHR15952, 1 hit |
Pfami | View protein in Pfam PF19282, Exportin-T, 2 hits PF08389, Xpo1, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | XPOT_YEAST | |
Accessioni | P33418Primary (citable) accession number: P33418 Secondary accession number(s): D6VWZ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - SIMILARITY comments
Index of protein domains and families