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UniProtKB - P33400 (PACC_YEAST)
Protein
pH-response transcription factor pacC/RIM101
Gene
RIM101
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Transcription factor that mediates regulation of both acid- and alkaline-expressed genes in response to ambient pH. At alkaline ambient pH, activates transcription of alkaline-expressed genes (including RIM101 itself), mainly by repressing transcriptional repressors of those genes, and represses transcription of acid-expressed genes. Required for meiosis, sporulation and invasive growth.
2 PublicationsMiscellaneous
Present with 1822 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 146 – 171 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 182 – 206 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 212 – 234 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: SGD
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- ascospore formation Source: SGD
- cellular response to alkaline pH Source: SGD
- cellular response to anoxia Source: SGD
- fungal-type cell wall biogenesis Source: SGD
- meiotic cell cycle Source: SGD
- negative regulation of transcription by RNA polymerase II Source: SGD
- positive regulation of division septum assembly Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Meiosis, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: pH-response transcription factor pacC/RIM101Alternative name(s): Regulator of IME2 protein 1 pH-response regulator protein RIM101 |
Gene namesi | Name:RIM101 Synonyms:RIM1 Ordered Locus Names:YHL027W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001019, RIM101 |
VEuPathDBi | FungiDB:YHL027W |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 153 | C → S: Abolishes transcriptional activation of target genes. 1 Publication | 1 | |
Mutagenesisi | 189 | C → S: Abolishes transcriptional activation of target genes. 1 Publication | 1 | |
Mutagenesisi | 217 | C → S: Abolishes transcriptional activation of target genes. 1 Publication | 1 | |
Mutagenesisi | 255 | S → A: Reduces transcriptional activation of target genes. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046847 | 1 – 625 | pH-response transcription factor pacC/RIM101Add BLAST | 625 |
Post-translational modificationi
Activated by C-terminal proteolytic cleavage. At neutral to alkaline ambient pH, the signaling protease (probably RIM13) cleaves RIM101, removing a C-terminal 8 kDa peptide to yield the active form.1 Publication
Proteomic databases
MaxQBi | P33400 |
PaxDbi | P33400 |
PRIDEi | P33400 |
PTM databases
iPTMneti | P33400 |
Interactioni
Subunit structurei
Binds to DNA.
Interacts with RIM20, which probably binds to the two YPX[LI] motifs and is required for proteolytic processing.
1 PublicationBinary interactionsi
P33400
With | #Exp. | IntAct |
---|---|---|
LSB3 [P43603] | 2 | EBI-14422,EBI-22980 |
RIM20 [Q12033] | 3 | EBI-14422,EBI-38947 |
YSC84 [P32793] | 2 | EBI-14422,EBI-24460 |
Protein-protein interaction databases
BioGRIDi | 36395, 476 interactors |
DIPi | DIP-1564N |
IntActi | P33400, 10 interactors |
MINTi | P33400 |
STRINGi | 4932.YHL027W |
Miscellaneous databases
RNActi | P33400, protein |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 138 | DisorderedSequence analysisAdd BLAST | 138 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 228 – 234 | Nuclear localization signalBy similarity | 7 | |
Motifi | 450 – 453 | YPX[LI] motif 1 | 4 | |
Motifi | 620 – 623 | YPX[LI] motif 2 | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 13 – 49 | Polar residuesSequence analysisAdd BLAST | 37 | |
Compositional biasi | 73 – 97 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 104 – 125 | Polar residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the pacC/RIM101 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 146 – 171 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 182 – 206 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 25 | |
Zinc fingeri | 212 – 234 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
HOGENOMi | CLU_029652_0_0_1 |
InParanoidi | P33400 |
OMAi | TIANATQ |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 2 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
i Sequence
Sequence statusi: Complete.
P33400-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVPLEDLLNK ENGTAAPQHS RESIVENGTD VSNVTKKDGL PSPNLSKRSS
60 70 80 90 100
DCSKRPRIRC TTEAIGLNGQ EDERMSPGST SSSCLPYHST SHLNTPPYDL
110 120 130 140 150
LGASAVSPTT SSSSDSSSSS PLAQAHNPAG DDDDADNDGD SEDITLYCKW
160 170 180 190 200
DNCGMIFNQP ELLYNHLCHD HVGRKSHKNL QLNCHWGDCT TKTEKRDHIT
210 220 230 240 250
SHLRVHVPLK PFGCSTCSKK FKRPQDLKKH LKIHLESGGI LKRKRGPKWG
260 270 280 290 300
SKRTSKKNKS CASDAVSSCS ASVPSAIAGS FKSHSTSPQI LPPLPVGISQ
310 320 330 340 350
HLPSQQQQRA ISLNQLCSDE LSQYKPVYSP QLSARLQTIL PPLYYNNGST
360 370 380 390 400
VSQGANSRSM NVYEDGCSNK TIANATQFFT KLSRNMTNNY ILQQSGGSTE
410 420 430 440 450
SSSSSGRIPV AQTSYVQPPN APSYQSVQGG SSISATANTA TYVPVRLAKY
460 470 480 490 500
PTGPSLTEHL PPLHSNTAGG VFNRQSQYAM PHYPSVRAAP SYSSSGCSIL
510 520 530 540 550
PPLQSKIPML PSRRTMAGGT SLKPNWEFSL NQKSCTNDII MSKLAIEEVD
560 570 580 590 600
DESEIEDDFV EMLGIVNIIK DYLLCCVMED LDDEESEDKD EENAFLQESL
610 620
EKLSLQNQMG TNSVRILTKY PKILV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 68 | N → K in CAA51462 (PubMed:8367297).Curated | 1 | |
Sequence conflicti | 90 | T → S in CAA51462 (PubMed:8367297).Curated | 1 | |
Sequence conflicti | 111 | S → P in CAA51462 (PubMed:8367297).Curated | 1 | |
Sequence conflicti | 249 | W → L in CAA51462 (PubMed:8367297).Curated | 1 | |
Sequence conflicti | 261 | C → S in CAA51462 (PubMed:8367297).Curated | 1 | |
Sequence conflicti | 276 | A → G in CAA51462 (PubMed:8367297).Curated | 1 | |
Sequence conflicti | 308 | Q → QQQQ (PubMed:8367297).Curated | 1 | |
Sequence conflicti | 358 | R → Q in CAA51462 (PubMed:8367297).Curated | 1 | |
Sequence conflicti | 486 | V → I in CAA51462 (PubMed:8367297).Curated | 1 | |
Sequence conflicti | 519 | G → E in CAA51462 (PubMed:8367297).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72960 Genomic DNA Translation: CAA51462.1 U11583 Genomic DNA Translation: AAB65039.1 AY693138 Genomic DNA Translation: AAT93157.1 BK006934 Genomic DNA Translation: DAA06658.1 |
PIRi | S48941 |
RefSeqi | NP_011836.1, NM_001179107.1 |
Genome annotation databases
EnsemblFungii | YHL027W_mRNA; YHL027W; YHL027W |
GeneIDi | 856358 |
KEGGi | sce:YHL027W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72960 Genomic DNA Translation: CAA51462.1 U11583 Genomic DNA Translation: AAB65039.1 AY693138 Genomic DNA Translation: AAT93157.1 BK006934 Genomic DNA Translation: DAA06658.1 |
PIRi | S48941 |
RefSeqi | NP_011836.1, NM_001179107.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 36395, 476 interactors |
DIPi | DIP-1564N |
IntActi | P33400, 10 interactors |
MINTi | P33400 |
STRINGi | 4932.YHL027W |
PTM databases
iPTMneti | P33400 |
Proteomic databases
MaxQBi | P33400 |
PaxDbi | P33400 |
PRIDEi | P33400 |
Genome annotation databases
EnsemblFungii | YHL027W_mRNA; YHL027W; YHL027W |
GeneIDi | 856358 |
KEGGi | sce:YHL027W |
Organism-specific databases
SGDi | S000001019, RIM101 |
VEuPathDBi | FungiDB:YHL027W |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
HOGENOMi | CLU_029652_0_0_1 |
InParanoidi | P33400 |
OMAi | TIANATQ |
Miscellaneous databases
PROi | PR:P33400 |
RNActi | P33400, protein |
Family and domain databases
InterProi | View protein in InterPro IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00096, zf-C2H2, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits |
SUPFAMi | SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 2 hits PS50157, ZINC_FINGER_C2H2_2, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PACC_YEAST | |
Accessioni | P33400Primary (citable) accession number: P33400 Secondary accession number(s): D3DKU1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 188 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VIII
Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names - SIMILARITY comments
Index of protein domains and families