UniProtKB - P33361 (YEHY_ECOLI)
Protein
Glycine betaine uptake system permease protein YehY
Gene
yehY
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of an ABC transporter complex involved in low-affinity glycine betaine uptake. Probably responsible for the translocation of the substrate across the membrane.1 Publication
Caution
Was originally thought to be part of an osmoprotectant uptake system (PubMed:15251200). However, it was shown later that the complex does not mediate osmotic stress protection (PubMed:26325238).2 Publications
GO - Biological processi
- amino acid transport Source: UniProtKB-KW
- glycine betaine transport Source: EcoCyc
- transmembrane transport Source: InterPro
Keywordsi
Biological process | Amino-acid transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:YEHY-MONOMER MetaCyc:YEHY-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.12.15, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Glycine betaine uptake system permease protein YehYCurated |
Gene namesi | Name:yehY Ordered Locus Names:b2130, JW2118 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 5 | CytoplasmicCurated | 5 | |
Transmembranei | 6 – 26 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 27 – 44 | PeriplasmicCuratedAdd BLAST | 18 | |
Transmembranei | 45 – 65 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 66 – 69 | CytoplasmicCurated | 4 | |
Transmembranei | 70 – 90 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 91 – 106 | PeriplasmicCuratedAdd BLAST | 16 | |
Transmembranei | 107 – 127 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 128 – 137 | CytoplasmicCurated | 10 | |
Transmembranei | 138 – 158 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 159 – 180 | PeriplasmicCuratedAdd BLAST | 22 | |
Transmembranei | 181 – 201 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 202 – 224 | CytoplasmicCuratedAdd BLAST | 23 | |
Transmembranei | 225 – 245 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 246 – 247 | PeriplasmicCurated | 2 | |
Transmembranei | 248 – 268 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 269 – 322 | CytoplasmicCuratedAdd BLAST | 54 | |
Transmembranei | 323 – 343 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 344 – 345 | PeriplasmicCurated | 2 | |
Transmembranei | 346 – 366 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 367 – 385 | Cytoplasmic1 PublicationAdd BLAST | 19 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000060256 | 1 – 385 | Glycine betaine uptake system permease protein YehYAdd BLAST | 385 |
Proteomic databases
PaxDbi | P33361 |
PRIDEi | P33361 |
Expressioni
Inductioni
Expression is sigma S-dependent. Induced by both osmotic shock and entry into stationary phase.1 Publication
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (YehX), two transmembrane proteins (YehW and YehY) and a solute-binding protein (YehZ).
CuratedBinary interactionsi
P33361
With | #Exp. | IntAct |
---|---|---|
bolA [P0ABE2] | 3 | EBI-545876,EBI-545774 |
Protein-protein interaction databases
BioGRIDi | 4260449, 12 interactors 851002, 3 interactors |
ComplexPortali | CPX-4449, Low affinity betaine ABC transporter complex |
DIPi | DIP-11913N |
IntActi | P33361, 2 interactors |
STRINGi | 511145.b2130 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 177 – 372 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 196 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1174, Bacteria |
HOGENOMi | CLU_046113_3_0_6 |
InParanoidi | P33361 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit |
InterProi | View protein in InterPro IPR000515, MetI-like IPR035906, MetI-like_sf |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
i Sequence
Sequence statusi: Complete.
P33361-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTYFRINPVL ALLLLLTAIA AALPFISYAP NRLVSGEGRH LWQLWPQTIW
60 70 80 90 100
MLVGVGCAWL TACFIPGKKG SICALILAQF VFVLLVWGAG KAATQLAQNG
110 120 130 140 150
SALARTSLGS GFWLAAALAL LACSDAIRRI STHPLWRWLL HMQIAIIPLW
160 170 180 190 200
LLYSGTLNDL SLMKEYANRQ DVFDDALAQH LTLLFGAVLP ALVIGVPLGI
210 220 230 240 250
WCYFSTARQG AIFSLLNVIQ TVPSVALFGL LIAPLAALVT AFPWLGTLGI
260 270 280 290 300
AGTGMTPALI ALVLYALLPL VRGVVVGLNQ IPRDVLESAR AMGMSGAQRF
310 320 330 340 350
LHVQLPLALP VFLRSLRVVM VQTVGMAVIA ALIGAGGFGA LVFQGLLSSA
360 370 380
IDLVLLGVIP VIVLAVLTDA LFDLLIALLK VKRND
Sequence cautioni
The sequence AAA60493 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 346 – 385 | LLSSA…VKRND → AAKQRH in AAA60493 (Ref. 1) CuratedAdd BLAST | 40 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00007 Genomic DNA Translation: AAA60493.1 Different initiation. U00096 Genomic DNA Translation: AAC75191.1 AP009048 Genomic DNA Translation: BAE76607.1 |
PIRi | A64981 |
RefSeqi | NP_416634.1, NC_000913.3 WP_000220837.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75191; AAC75191; b2130 BAE76607; BAE76607; BAE76607 |
GeneIDi | 946660 |
KEGGi | ecj:JW2118 eco:b2130 |
PATRICi | fig|1411691.4.peg.114 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00007 Genomic DNA Translation: AAA60493.1 Different initiation. U00096 Genomic DNA Translation: AAC75191.1 AP009048 Genomic DNA Translation: BAE76607.1 |
PIRi | A64981 |
RefSeqi | NP_416634.1, NC_000913.3 WP_000220837.1, NZ_LN832404.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260449, 12 interactors 851002, 3 interactors |
ComplexPortali | CPX-4449, Low affinity betaine ABC transporter complex |
DIPi | DIP-11913N |
IntActi | P33361, 2 interactors |
STRINGi | 511145.b2130 |
Protein family/group databases
TCDBi | 3.A.1.12.15, the atp-binding cassette (abc) superfamily |
Proteomic databases
PaxDbi | P33361 |
PRIDEi | P33361 |
Genome annotation databases
EnsemblBacteriai | AAC75191; AAC75191; b2130 BAE76607; BAE76607; BAE76607 |
GeneIDi | 946660 |
KEGGi | ecj:JW2118 eco:b2130 |
PATRICi | fig|1411691.4.peg.114 |
Organism-specific databases
EchoBASEi | EB1949 |
Phylogenomic databases
eggNOGi | COG1174, Bacteria |
HOGENOMi | CLU_046113_3_0_6 |
InParanoidi | P33361 |
Enzyme and pathway databases
BioCyci | EcoCyc:YEHY-MONOMER MetaCyc:YEHY-MONOMER |
Miscellaneous databases
PROi | PR:P33361 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit |
InterProi | View protein in InterPro IPR000515, MetI-like IPR035906, MetI-like_sf |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YEHY_ECOLI | |
Accessioni | P33361Primary (citable) accession number: P33361 Secondary accession number(s): P76435, Q2MAU9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families