UniProtKB - P33360 (YEHX_ECOLI)
Protein
Glycine betaine uptake system ATP-binding protein YehX
Gene
yehX
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of an ABC transporter complex involved in low-affinity glycine betaine uptake. Probably responsible for energy coupling to the transport system.1 Publication
Caution
Was originally thought to be part of an osmoprotectant uptake system (PubMed:15251200). However, it was shown later that the complex does not mediate osmotic stress protection (PubMed:26325238).2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 34 – 41 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- amino acid transport Source: UniProtKB-KW
- glycine betaine transport Source: EcoCyc
Keywordsi
Molecular function | Translocase |
Biological process | Amino-acid transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:YEHX-MONOMER MetaCyc:YEHX-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.12.15, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:yehX Ordered Locus Names:b2129, JW2117 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093167 | 1 – 308 | Glycine betaine uptake system ATP-binding protein YehXAdd BLAST | 308 |
Proteomic databases
jPOSTi | P33360 |
PaxDbi | P33360 |
PRIDEi | P33360 |
Expressioni
Inductioni
Expression is sigma S-dependent. Induced by both osmotic shock and entry into stationary phase.1 Publication
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (YehX), two transmembrane proteins (YehW and YehY) and a solute-binding protein (YehZ).
CuratedProtein-protein interaction databases
BioGRIDi | 4260448, 33 interactors 851001, 1 interactor |
ComplexPortali | CPX-4449, Low affinity betaine ABC transporter complex |
DIPi | DIP-11912N |
IntActi | P33360, 8 interactors |
STRINGi | 511145.b2129 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 235 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 234 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily.Curated
Phylogenomic databases
eggNOGi | COG1125, Bacteria |
HOGENOMi | CLU_000604_1_5_6 |
InParanoidi | P33360 |
PhylomeDBi | P33360 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P33360-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIEFSHVSKL FGAQKAVNDL NLNFQEGSFS VLIGTSGSGK STTLKMINRL
60 70 80 90 100
VEHDSGEIRF AGEEIRSLPV LELRRRMGYA IQSIGLFPHW SVAQNIATVP
110 120 130 140 150
QLQKWSRARI DDRIDELMAL LGLESNLRER YPHQLSGGQQ QRVGVARALA
160 170 180 190 200
ADPQVLLMDE PFGALDPVTR GALQQEMTRI HRLLGRTIVL VTHDIDEALR
210 220 230 240 250
LAEHLVLMDH GEVVQQGNPL TMLTRPANDF VRQFFGRSEL GVRLLSLRSV
260 270 280 290 300
ADYVRREERA DGEALAEEMT LRDALSLFVA RGCEVLPVVN MQGQPCGTLH
FQDLLVEA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00007 Genomic DNA Translation: AAA60492.1 U00096 Genomic DNA Translation: AAC75190.1 AP009048 Genomic DNA Translation: BAE76606.1 |
PIRi | H64980 |
RefSeqi | NP_416633.1, NC_000913.3 WP_000569315.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75190; AAC75190; b2129 BAE76606; BAE76606; BAE76606 |
GeneIDi | 946659 |
KEGGi | ecj:JW2117 eco:b2129 |
PATRICi | fig|1411691.4.peg.115 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00007 Genomic DNA Translation: AAA60492.1 U00096 Genomic DNA Translation: AAC75190.1 AP009048 Genomic DNA Translation: BAE76606.1 |
PIRi | H64980 |
RefSeqi | NP_416633.1, NC_000913.3 WP_000569315.1, NZ_LN832404.1 |
3D structure databases
SMRi | P33360 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260448, 33 interactors 851001, 1 interactor |
ComplexPortali | CPX-4449, Low affinity betaine ABC transporter complex |
DIPi | DIP-11912N |
IntActi | P33360, 8 interactors |
STRINGi | 511145.b2129 |
Protein family/group databases
TCDBi | 3.A.1.12.15, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P33360 |
PaxDbi | P33360 |
PRIDEi | P33360 |
Genome annotation databases
EnsemblBacteriai | AAC75190; AAC75190; b2129 BAE76606; BAE76606; BAE76606 |
GeneIDi | 946659 |
KEGGi | ecj:JW2117 eco:b2129 |
PATRICi | fig|1411691.4.peg.115 |
Organism-specific databases
EchoBASEi | EB1948 |
Phylogenomic databases
eggNOGi | COG1125, Bacteria |
HOGENOMi | CLU_000604_1_5_6 |
InParanoidi | P33360 |
PhylomeDBi | P33360 |
Enzyme and pathway databases
BioCyci | EcoCyc:YEHX-MONOMER MetaCyc:YEHX-MONOMER |
Miscellaneous databases
PROi | PR:P33360 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YEHX_ECOLI | |
Accessioni | P33360Primary (citable) accession number: P33360 Secondary accession number(s): Q2MAV0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families