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UniProtKB - P33333 (PLSC_YEAST)
Protein
1-acyl-sn-glycerol-3-phosphate acyltransferase
Gene
SLC1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Acyltransferase that catalyzes the sn-2-specific, acyl-CoA-dependent acylation of lysophosphatidic acid (LPA) to phosphatidic acid (PA) in lipid particles (PubMed:9401016).
Together with ALE1, plays a central role in PA biosynthesis. PA is the intermediate, from which all glycerophospholipids are synthesized (PubMed:17890783).
Can also acylate lysophosphoinositol (LPI) and lysophosphoserine (LPS) (PubMed:17675291).
The fatty acyl substrates include 18:1-acyl-CoA, 14:0-acyl-CoA, 12:0-acyl-CoA and 10:0-acyl-CoA (PubMed:20694142).
4 PublicationsCatalytic activityi
- a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA3 PublicationsEC:2.3.1.513 Publications
- a 1-acyl-sn-glycero-3-phosphocholine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphocholine + CoA1 PublicationEC:2.3.1.231 PublicationThis reaction proceeds in the forward1 Publication direction.
- a 1-acyl-sn-glycero-3-phosphoethanolamine + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CoA1 PublicationEC:2.3.1.n71 Publication
- (9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphate = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-octadecanoyl-sn-glycero-3-phosphate = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine = 1,2-di-(9Z)-octadecenoyl-sn-glycero-3-phospho-L-serine + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) = a 1-acyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1D-myo-inositol) + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z)-octadecenoyl-CoA + 1-heptadecanoyl-sn-glycero-3-phosphate = 1-heptadecanoyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-heptadecanoyl-sn-glycero-3-phosphate + dodecanoyl-CoA = 1-heptadecanoyl-2-dodecanoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
- 1-heptadecanoyl-sn-glycero-3-phosphate + tetradecanoyl-CoA = 1-heptadecanoyl-2-tetradecanoyl-sn-glycero-3-phosphate + CoA1 PublicationThis reaction proceeds in the forward1 Publication direction.
: CDP-diacylglycerol biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate. This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.
GO - Molecular functioni
- 1-acylglycerol-3-phosphate O-acyltransferase activity Source: SGD
- 1-acylglycerophosphocholine O-acyltransferase activity Source: UniProtKB-EC
- 1-acylglycerophosphoethanolamine O-acyltransferase activity Source: RHEA
GO - Biological processi
- CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
- glycerophospholipid biosynthetic process Source: SGD
- phosphatidic acid biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Enzyme and pathway databases
BRENDAi | 2.3.1.51, 984 |
Reactomei | R-SCE-1483166, Synthesis of PA R-SCE-163765, ChREBP activates metabolic gene expression R-SCE-6798695, Neutrophil degranulation |
UniPathwayi | UPA00557;UER00613 |
Chemistry databases
SwissLipidsi | SLP:000000049 |
Names & Taxonomyi
Protein namesi | Recommended name: 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC:2.3.1.231 Publication, EC:2.3.1.513 Publications, EC:2.3.1.n71 Publication)Short name: 1-AGP acyltransferase Short name: 1-AGPAT Alternative name(s): Lysophosphatidic acid acyltransferase Short name: LPAAT Sphingolipid compensation protein 11 Publication |
Gene namesi | Name:SLC11 Publication Ordered Locus Names:YDL052C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002210, SLC1 |
VEuPathDBi | FungiDB:YDL052C |
Subcellular locationi
Other locations
- Lipid droplet 3 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Other locations
- lipid droplet Source: SGD
- membrane Source: InterPro
Keywords - Cellular componenti
Lipid dropletPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208189 | 1 – 303 | 1-acyl-sn-glycerol-3-phosphate acyltransferaseAdd BLAST | 303 |
Proteomic databases
MaxQBi | P33333 |
PaxDbi | P33333 |
PRIDEi | P33333 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 32007, 111 interactors |
DIPi | DIP-5136N |
IntActi | P33333, 43 interactors |
MINTi | P33333 |
STRINGi | 4932.YDL052C |
Miscellaneous databases
RNActi | P33333, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 278 – 303 | DisorderedSequence analysisAdd BLAST | 26 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 82 – 87 | HXXXXD motif | 6 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 280 – 295 | Polar residuesSequence analysisAdd BLAST | 16 |
Domaini
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity
Sequence similaritiesi
Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.Curated
Phylogenomic databases
eggNOGi | KOG2848, Eukaryota |
GeneTreei | ENSGT00390000008726 |
HOGENOMi | CLU_027938_10_0_1 |
InParanoidi | P33333 |
OMAi | RLMGITM |
Family and domain databases
InterProi | View protein in InterPro IPR004552, AGP_acyltrans IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
TIGRFAMsi | TIGR00530, AGP_acyltrn, 1 hit |
i Sequence
Sequence statusi: Complete.
P33333-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVIGRFLYY LRSVLVVLAL AGCGFYGVIA SILCTLIGKQ HLAQWITARC
60 70 80 90 100
FYHVMKLMLG LDVKVVGEEN LAKKPYIMIA NHQSTLDIFM LGRIFPPGCT
110 120 130 140 150
VTAKKSLKYV PFLGWFMALS GTYFLDRSKR QEAIDTLNKG LENVKKNKRA
160 170 180 190 200
LWVFPEGTRS YTSELTMLPF KKGAFHLAQQ GKIPIVPVVV SNTSTLVSPK
210 220 230 240 250
YGVFNRGCMI VRILKPISTE NLTKDKIGEF AEKVRDQMVD TLKEIGYSPA
260 270 280 290 300
INDTTLPPQA IEYAALQHDK KVNKKIKNEP VPSVSISNDV NTHNEGSSVK
KMH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 115 | W → R in AAT92911 (PubMed:17322287).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 44 | Q → L in allele suppressor SLC1-1. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13282 Unassigned DNA Translation: AAA16514.1 Z74100 Genomic DNA Translation: CAA98614.1 AY692892 Genomic DNA Translation: AAT92911.1 BK006938 Genomic DNA Translation: DAA11804.1 |
PIRi | A48600 |
RefSeqi | NP_010231.1, NM_001180111.1 |
Genome annotation databases
EnsemblFungii | YDL052C_mRNA; YDL052C; YDL052C |
GeneIDi | 851508 |
KEGGi | sce:YDL052C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L13282 Unassigned DNA Translation: AAA16514.1 Z74100 Genomic DNA Translation: CAA98614.1 AY692892 Genomic DNA Translation: AAT92911.1 BK006938 Genomic DNA Translation: DAA11804.1 |
PIRi | A48600 |
RefSeqi | NP_010231.1, NM_001180111.1 |
3D structure databases
AlphaFoldDBi | P33333 |
SMRi | P33333 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 32007, 111 interactors |
DIPi | DIP-5136N |
IntActi | P33333, 43 interactors |
MINTi | P33333 |
STRINGi | 4932.YDL052C |
Chemistry databases
SwissLipidsi | SLP:000000049 |
Proteomic databases
MaxQBi | P33333 |
PaxDbi | P33333 |
PRIDEi | P33333 |
Genome annotation databases
EnsemblFungii | YDL052C_mRNA; YDL052C; YDL052C |
GeneIDi | 851508 |
KEGGi | sce:YDL052C |
Organism-specific databases
SGDi | S000002210, SLC1 |
VEuPathDBi | FungiDB:YDL052C |
Phylogenomic databases
eggNOGi | KOG2848, Eukaryota |
GeneTreei | ENSGT00390000008726 |
HOGENOMi | CLU_027938_10_0_1 |
InParanoidi | P33333 |
OMAi | RLMGITM |
Enzyme and pathway databases
UniPathwayi | UPA00557;UER00613 |
BRENDAi | 2.3.1.51, 984 |
Reactomei | R-SCE-1483166, Synthesis of PA R-SCE-163765, ChREBP activates metabolic gene expression R-SCE-6798695, Neutrophil degranulation |
Miscellaneous databases
PROi | PR:P33333 |
RNActi | P33333, protein |
Family and domain databases
InterProi | View protein in InterPro IPR004552, AGP_acyltrans IPR002123, Plipid/glycerol_acylTrfase |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
TIGRFAMsi | TIGR00530, AGP_acyltrn, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PLSC_YEAST | |
Accessioni | P33333Primary (citable) accession number: P33333 Secondary accession number(s): D6VRU4, E9P8Y5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families