UniProtKB - P33332 (SEC3_YEAST)
Protein
Exocyst complex component SEC3
Gene
SEC3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Miscellaneous
Present with 377 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- GTP-Rho binding Source: SGD
- phosphatidylinositol-4,5-bisphosphate binding Source: SGD
GO - Biological processi
- endoplasmic reticulum inheritance Source: SGD
- exocyst assembly Source: SGD
- exocyst localization Source: SGD
- exocytosis Source: SGD
- Golgi to plasma membrane transport Source: SGD
- protein transport Source: UniProtKB-KW
- vesicle tethering involved in exocytosis Source: SGD
Keywordsi
Biological process | Exocytosis, Protein transport, Transport |
Enzyme and pathway databases
BioCyci | YEAST:G3O-30195-MONOMER |
Reactomei | R-SCE-264876 Insulin processing |
Protein family/group databases
TCDBi | 1.F.2.1.1 the octameric exocyst (exocyst) family |
Names & Taxonomyi
Protein namesi | Recommended name: Exocyst complex component SEC3Alternative name(s): Protein PSL1 |
Gene namesi | Name:SEC3 Synonyms:PSL1 Ordered Locus Names:YER008C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YER008C |
SGDi | S000000810 SEC3 |
Subcellular locationi
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- cell Source: GOC
- cellular bud neck Source: SGD
- cellular bud tip Source: SGD
- cytoplasm Source: SGD
- exocyst Source: SGD
- incipient cellular bud site Source: SGD
- mating projection tip Source: SGD
- prospore membrane Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118917 | 1 – 1336 | Exocyst complex component SEC3Add BLAST | 1336 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 290 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 606 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P33332 |
PaxDbi | P33332 |
PRIDEi | P33332 |
PTM databases
iPTMneti | P33332 |
Interactioni
Subunit structurei
The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84.
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
P06780 | 2 | EBI-16850,EBI-15121 |
GO - Molecular functioni
- GTP-Rho binding Source: SGD
Protein-protein interaction databases
BioGridi | 36739, 175 interactors |
ComplexPortali | CPX-1890 Exocyst |
DIPi | DIP-5992N |
IntActi | P33332, 17 interactors |
MINTi | P33332 |
STRINGi | 4932.YER008C |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P33332 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P33332 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 319 – 464 | Sequence analysisAdd BLAST | 146 | |
Coiled coili | 1309 – 1336 | Sequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Belongs to the SEC3 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
HOGENOMi | HOG000142343 |
InParanoidi | P33332 |
KOi | K19983 |
OMAi | KIYRKYT |
Family and domain databases
InterProi | View protein in InterPro IPR028258 Sec3-PIP2_bind IPR019160 Sec3_C |
Pfami | View protein in Pfam PF15277 Sec3-PIP2_bind, 1 hit PF09763 Sec3_C, 1 hit |
SMARTi | View protein in SMART SM01313 Sec3-PIP2_bind, 1 hit |
i Sequence
Sequence statusi: Complete.
P33332-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRSSKSPFKR KSHSRETSHD ENTSFFHKRT ISGSSAHHSR NVSQGAVPSS
60 70 80 90 100
APPVSGGNYS HKRNVSRASN SSQTSNFLAE QYERDRKAII NCCFSRPDHK
110 120 130 140 150
TGEPPNNYIT HVRIIEDSKF PSSRPPPDSK LENKKKRLLI LSAKPNNAKL
160 170 180 190 200
IQIHKARENS DGSFQIGRTW QLTELVRVEK DLEISEGFIL TMSKKYYWET
210 220 230 240 250
NSAKERTVFI KSLITLYIQT FEGHVPELVN WDLSLFYLDE RSYQRAVITN
260 270 280 290 300
RPGSVSPIKS PTSNFTTNTT QSVGSVPFSA PTERTRRSET ESVNPVSTPA
310 320 330 340 350
SVEYHAGMKS LNKAPYSSNS TLNEVNKRYE LEQQQQQEEA ELRRLEEQKR
360 370 380 390 400
LQLQKENEMK RLEEERRIKQ EERKRQMELE HQRQLEEEER KRQMELEAKK
410 420 430 440 450
QMELKRQRQF EEEQRLKKER ELLEIQRKQR EQETAERLKK EEQEALAKKE
460 470 480 490 500
EEEKSKRNKV DNESYTQEIN GKVDNLLEDL NAVLAEETET TPTMQNGTYV
510 520 530 540 550
PERSTARAHD QLKKPLNIAK VESLGGSDLN DSISLSDEIA GLNTSNLSGE
560 570 580 590 600
DQDEKNDLSF EKGDEVRYSN NFEGEAPHVY HEVSIIQEEA PAVSQKLILP
610 620 630 640 650
EENNESEALI ESKEEIKTME NIDDEVLLEI LTDINWSIED DADSMIERID
660 670 680 690 700
LRLAETEYLF NQNLLSLQKI GPNIRPYEDK VNDECHRIIP TLSLFLMEMS
710 720 730 740 750
NFSNDIENVE SQDNGLQVES ANKKLLWNTL DELLKTVSLD EISLNQLLEC
760 770 780 790 800
PIREKNLPWM ENQLNLLLKA FQAIGSDGNE VEYNLREISG LKQRLQFYEK
810 820 830 840 850
VTKIFLNRIV EEMQKKFSNI RGQDISHDQM IRILTTLLIF SPLILFCKEI
860 870 880 890 900
SQKSYQAIVE NWNVSIQPVY MELWTKKISQ LQGIDTNDEK MNELSLSQLL
910 920 930 940 950
NEWDTFRKER KTNDINPVFK NSFSLLTECL QTMRQECIVY QNFVEVFFHI
960 970 980 990 1000
SSKHNFEEYI KHFNDPDAPP ILLDTVKVMQ SDREAAVIET QLVSRIFQPI
1010 1020 1030 1040 1050
VTRLSSYFVE LVKAEPTVAP ALTFYLENEI KSLESSNHEF LLSAVTRMYT
1060 1070 1080 1090 1100
QIKQVWSDNV EEQVLHFERI SNATTNGEIL PGILDLPVGL KNSEDLFQFA
1110 1120 1130 1140 1150
KRSMDIKDTD EGYESIELMN SSFRKLSIAA TRSITHKEVN SSINPNLSDT
1160 1170 1180 1190 1200
AALNNDYMET ISLLVNSNWL TEMLSMLNFN KDGIFDTSLQ NVKKVFDVEK
1210 1220 1230 1240 1250
ESYASFLLRD TMPKLTAFVY GVSNIIENTN NVNMTNPSRW AAYSRQNLEN
1260 1270 1280 1290 1300
ILLAYTSHEI ETLVKRLHTH MVNDFGYHQE NAINNVLCDK LWSCIQGQTV
1310 1320 1330
SLYLKLYTVI DKHYRGTNIR FTKNDIISAF EEYKNA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L22204 Genomic DNA Translation: AAB49380.1 U18778 Genomic DNA Translation: AAB64541.1 BK006939 Genomic DNA Translation: DAA07659.1 |
PIRi | S41794 |
RefSeqi | NP_010924.1, NM_001178899.1 |
Genome annotation databases
EnsemblFungii | YER008C_mRNA; YER008C; YER008C |
GeneIDi | 856726 |
KEGGi | sce:YER008C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L22204 Genomic DNA Translation: AAB49380.1 U18778 Genomic DNA Translation: AAB64541.1 BK006939 Genomic DNA Translation: DAA07659.1 |
PIRi | S41794 |
RefSeqi | NP_010924.1, NM_001178899.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3A58 | X-ray | 2.60 | A/C/E | 1-320 | [»] | |
3HIE | X-ray | 2.00 | A/B/C/D | 71-241 | [»] | |
5LG4 | X-ray | 2.90 | B/C | 75-320 | [»] | |
5M4Y | X-ray | 2.20 | B/D/F | 75-320 | [»] | |
5YFP | electron microscopy | 4.40 | A | 1-1336 | [»] | |
SMRi | P33332 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGridi | 36739, 175 interactors |
ComplexPortali | CPX-1890 Exocyst |
DIPi | DIP-5992N |
IntActi | P33332, 17 interactors |
MINTi | P33332 |
STRINGi | 4932.YER008C |
Protein family/group databases
TCDBi | 1.F.2.1.1 the octameric exocyst (exocyst) family |
PTM databases
iPTMneti | P33332 |
Proteomic databases
MaxQBi | P33332 |
PaxDbi | P33332 |
PRIDEi | P33332 |
Genome annotation databases
EnsemblFungii | YER008C_mRNA; YER008C; YER008C |
GeneIDi | 856726 |
KEGGi | sce:YER008C |
Organism-specific databases
EuPathDBi | FungiDB:YER008C |
SGDi | S000000810 SEC3 |
Phylogenomic databases
HOGENOMi | HOG000142343 |
InParanoidi | P33332 |
KOi | K19983 |
OMAi | KIYRKYT |
Enzyme and pathway databases
BioCyci | YEAST:G3O-30195-MONOMER |
Reactomei | R-SCE-264876 Insulin processing |
Miscellaneous databases
EvolutionaryTracei | P33332 |
PROi | PR:P33332 |
Family and domain databases
InterProi | View protein in InterPro IPR028258 Sec3-PIP2_bind IPR019160 Sec3_C |
Pfami | View protein in Pfam PF15277 Sec3-PIP2_bind, 1 hit PF09763 Sec3_C, 1 hit |
SMARTi | View protein in SMART SM01313 Sec3-PIP2_bind, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SEC3_YEAST | |
Accessioni | P33332Primary (citable) accession number: P33332 Secondary accession number(s): D3DLQ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | November 13, 2019 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - SIMILARITY comments
Index of protein domains and families - Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references