Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transketolase 2

Gene

TKL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.By similarity

Miscellaneous

Present with 149 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity, Ca2+By similarity, Mn2+By similarity, Co2+By similarityNote: Binds 1 Mg2+ ion per subunit. Can also utilize other divalent metal cations, such as Ca2+, Mn2+ and Co2+.By similarity
  • thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30SubstrateBy similarity1
Sitei30Important for catalytic activityBy similarity1
Binding sitei69Thiamine pyrophosphateBy similarity1
Metal bindingi157MagnesiumBy similarity1
Binding sitei158Thiamine pyrophosphate; via amide nitrogenBy similarity1
Metal bindingi187MagnesiumBy similarity1
Binding sitei187Thiamine pyrophosphateBy similarity1
Metal bindingi189Magnesium; via carbonyl oxygenBy similarity1
Binding sitei263SubstrateBy similarity1
Binding sitei263Thiamine pyrophosphateBy similarity1
Sitei263Important for catalytic activityBy similarity1
Binding sitei359SubstrateBy similarity1
Binding sitei386SubstrateBy similarity1
Active sitei418Proton donorBy similarity1
Binding sitei418Thiamine pyrophosphateBy similarity1
Binding sitei445Thiamine pyrophosphateBy similarity1
Binding sitei469SubstrateBy similarity1
Binding sitei477SubstrateBy similarity1
Binding sitei528SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi116 – 118Thiamine pyrophosphateBy similarity3

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • transketolase activity Source: SGD

GO - Biological processi

  • pentose-phosphate shunt Source: SGD

Keywordsi

Molecular functionTransferase
LigandCalcium, Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciYEAST:YBR117C-MONOMER
BRENDAi2.2.1.1 984
ReactomeiR-SCE-163754 Insulin effects increased synthesis of Xylulose-5-Phosphate
R-SCE-71336 Pentose phosphate pathway
SABIO-RKiP33315

Names & Taxonomyi

Protein namesi
Recommended name:
Transketolase 2 (EC:2.2.1.1)
Short name:
TK 2
Gene namesi
Name:TKL2
Ordered Locus Names:YBR117C
ORF Names:YBR0912
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

SGDiS000000321 TKL2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001919051 – 681Transketolase 2Add BLAST681

Proteomic databases

MaxQBiP33315
PaxDbiP33315
PRIDEiP33315
TopDownProteomicsiP33315

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi32819, 81 interactors
DIPiDIP-757N
IntActiP33315, 2 interactors
MINTiP33315
STRINGi4932.YBR117C

Structurei

3D structure databases

ProteinModelPortaliP33315
SMRiP33315
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transketolase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000005240
HOGENOMiHOG000225953
InParanoidiP33315
KOiK00615
OMAiDCITARV
OrthoDBiEOG092C0ZWN

Family and domain databases

Gene3Di3.40.50.920, 1 hit
InterProiView protein in InterPro
IPR029061 THDP-binding
IPR009014 Transketo_C/PFOR_II
IPR005475 Transketolase-like_Pyr-bd
IPR005478 Transketolase_bac-like
IPR020826 Transketolase_BS
IPR033248 Transketolase_C
IPR033247 Transketolase_fam
IPR005474 Transketolase_N
PANTHERiPTHR43522 PTHR43522, 1 hit
PfamiView protein in Pfam
PF02779 Transket_pyr, 1 hit
PF02780 Transketolase_C, 1 hit
PF00456 Transketolase_N, 1 hit
SMARTiView protein in SMART
SM00861 Transket_pyr, 1 hit
SUPFAMiSSF52518 SSF52518, 2 hits
SSF52922 SSF52922, 1 hit
TIGRFAMsiTIGR00232 tktlase_bact, 1 hit
PROSITEiView protein in PROSITE
PS00801 TRANSKETOLASE_1, 1 hit
PS00802 TRANSKETOLASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P33315-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAQFSDIDKL AVSTLRLLSV DQVESAQSGH PGAPLGLAPV AHVIFKQLRC
60 70 80 90 100
NPNNEHWINR DRFVLSNGHS CALLYSMLHL LGYDYSIEDL RQFRQVNSRT
110 120 130 140 150
PGHPEFHSAG VEITSGPLGQ GISNAVGMAI AQANFAATYN EDGFPISDSY
160 170 180 190 200
TFAIVGDGCL QEGVSSETSS LAGHLQLGNL ITFYDSNSIS IDGKTSYSFD
210 220 230 240 250
EDVLKRYEAY GWEVMEVDKG DDDMESISSA LEKAKLSKDK PTIIKVTTTI
260 270 280 290 300
GFGSLQQGTA GVHGSALKAD DVKQLKKRWG FDPNKSFVVP QEVYDYYKKT
310 320 330 340 350
VVEPGQKLNE EWDRMFEEYK TKFPEKGKEL QRRLNGELPE GWEKHLPKFT
360 370 380 390 400
PDDDALATRK TSQQVLTNMV QVLPELIGGS ADLTPSNLTR WEGAVDFQPP
410 420 430 440 450
ITQLGNYAGR YIRYGVREHG MGAIMNGISA FGANYKPYGG TFLNFVSYAA
460 470 480 490 500
GAVRLAALSG NPVIWVATHD SIGLGEDGPT HQPIETLAHL RAIPNMHVWR
510 520 530 540 550
PADGNETSAA YYSAIKSGRT PSVVALSRQN LPQLEHSSFE KALKGGYVIH
560 570 580 590 600
DVENPDIILV STGSEVSISI DAAKKLYDTK KIKARVVSLP DFYTFDRQSE
610 620 630 640 650
EYRFSVLPDG VPIMSFEVLA TSSWGKYAHQ SFGLDEFGRS GKGPEIYKLF
660 670 680
DFTADGVASR AEKTINYYKG KQLLSPMGRA F
Length:681
Mass (Da):75,029
Last modified:February 1, 1994 - v1
Checksum:i84D7477916D136D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73532 Genomic DNA Translation: CAA51937.1
X78993 Genomic DNA Translation: CAA55619.1
Z35985 Genomic DNA Translation: CAA85074.1
BK006936 Genomic DNA Translation: DAA07235.1
PIRiS37809
RefSeqiNP_009675.3, NM_001178465.3

Genome annotation databases

EnsemblFungiiYBR117C; YBR117C; YBR117C
GeneIDi852414
KEGGisce:YBR117C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73532 Genomic DNA Translation: CAA51937.1
X78993 Genomic DNA Translation: CAA55619.1
Z35985 Genomic DNA Translation: CAA85074.1
BK006936 Genomic DNA Translation: DAA07235.1
PIRiS37809
RefSeqiNP_009675.3, NM_001178465.3

3D structure databases

ProteinModelPortaliP33315
SMRiP33315
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32819, 81 interactors
DIPiDIP-757N
IntActiP33315, 2 interactors
MINTiP33315
STRINGi4932.YBR117C

Proteomic databases

MaxQBiP33315
PaxDbiP33315
PRIDEiP33315
TopDownProteomicsiP33315

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR117C; YBR117C; YBR117C
GeneIDi852414
KEGGisce:YBR117C

Organism-specific databases

SGDiS000000321 TKL2

Phylogenomic databases

GeneTreeiENSGT00390000005240
HOGENOMiHOG000225953
InParanoidiP33315
KOiK00615
OMAiDCITARV
OrthoDBiEOG092C0ZWN

Enzyme and pathway databases

BioCyciYEAST:YBR117C-MONOMER
BRENDAi2.2.1.1 984
ReactomeiR-SCE-163754 Insulin effects increased synthesis of Xylulose-5-Phosphate
R-SCE-71336 Pentose phosphate pathway
SABIO-RKiP33315

Miscellaneous databases

PROiPR:P33315

Family and domain databases

Gene3Di3.40.50.920, 1 hit
InterProiView protein in InterPro
IPR029061 THDP-binding
IPR009014 Transketo_C/PFOR_II
IPR005475 Transketolase-like_Pyr-bd
IPR005478 Transketolase_bac-like
IPR020826 Transketolase_BS
IPR033248 Transketolase_C
IPR033247 Transketolase_fam
IPR005474 Transketolase_N
PANTHERiPTHR43522 PTHR43522, 1 hit
PfamiView protein in Pfam
PF02779 Transket_pyr, 1 hit
PF02780 Transketolase_C, 1 hit
PF00456 Transketolase_N, 1 hit
SMARTiView protein in SMART
SM00861 Transket_pyr, 1 hit
SUPFAMiSSF52518 SSF52518, 2 hits
SSF52922 SSF52922, 1 hit
TIGRFAMsiTIGR00232 tktlase_bact, 1 hit
PROSITEiView protein in PROSITE
PS00801 TRANSKETOLASE_1, 1 hit
PS00802 TRANSKETOLASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTKT2_YEAST
AccessioniPrimary (citable) accession number: P33315
Secondary accession number(s): D6VQB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 7, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again