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Entry version 179 (07 Oct 2020)
Sequence version 2 (03 Oct 2006)
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Protein

Protein DOM34

Gene

DOM34

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in protein translation. Together with HBS1, may function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs. The complex formed by DOM34 and HBS1 has ribonuclease activity towards double-stranded RNA substrates, but does not cleave single-stranded RNA. Acts as endonuclease; has no exonuclease activity. Increases the affinity of HBS1 for GTP, but nor for GDP. Promotes G1 progression and differentiation and is involved in mitotic and meiotic cell divisions.3 Publications

Miscellaneous

Present with 1720 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

a divalent metal cation1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processCell cycle, Cell division, Meiosis, Mitosis, Protein biosynthesis, Translation regulation
LigandMetal-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Protein DOM34 (EC:3.1.-.-)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:DOM34
Ordered Locus Names:YNL001W
ORF Names:N2016
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XIV

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YNL001W

Saccharomyces Genome Database

More...
SGDi
S000004946, DOM34

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001431911 – 386Protein DOM34Add BLAST386

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P33309

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P33309

PRoteomics IDEntifications database

More...
PRIDEi
P33309

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer.

Interacts with HBS1.

3 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
35824, 288 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-465, DOM34-HBS1 ribosome dissociation complex

Database of interacting proteins

More...
DIPi
DIP-7520N

Protein interaction database and analysis system

More...
IntActi
P33309, 15 interactors

Molecular INTeraction database

More...
MINTi
P33309

STRING: functional protein association networks

More...
STRINGi
4932.YNL001W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P33309, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1386
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P33309

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P33309

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity.

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2869, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000016326

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_023334_3_1_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P33309

KEGG Orthology (KO)

More...
KOi
K06965

Identification of Orthologs from Complete Genome Data

More...
OMAi
FAHERMA

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.30.30.870, 1 hit
3.30.1330.30, 1 hit
3.30.420.60, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR042226, eFR1_2_sf
IPR005140, eRF1_1_Pelota
IPR005141, eRF1_2
IPR005142, eRF1_3
IPR029064, L30e-like
IPR038069, Pelota/DOM34_N
IPR004405, Transl-rel_pelota

The PANTHER Classification System

More...
PANTHERi
PTHR10853, PTHR10853, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03463, eRF1_1, 1 hit
PF03464, eRF1_2, 1 hit
PF03465, eRF1_3, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01194, eRF1_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF159065, SSF159065, 1 hit
SSF55315, SSF55315, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00111, pelota, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P33309-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVISLKKDS FNKGGAVITL LPEDKEDLFT VYQIVDKDDE LIFKKKFTSK
60 70 80 90 100
LDEAGKKKST DLVKLKIKVI SEDFDMKDEY LKYKGVTVTD ESGASNVDIP
110 120 130 140 150
VGKYLSFTLD YVYPFTIIKQ NFNKFMQKLL NEACNIEYKS DTAAVVLQEG
160 170 180 190 200
IAHVCLVTSS STILKQKIEY SMPKKKRTTD VLKFDEKTEK FYKAIYSAMK
210 220 230 240 250
KDLNFDKLKT IILCSPGFYA KILMDKIFQY AEEEHNKKIL DNKGMFFIAH
260 270 280 290 300
CSTGYLQGIN EVLKNPLYAS KLQDTKYSKE IMVMDEFLLH LNKDDDKAWY
310 320 330 340 350
GEKEVVKAAE YGAISYLLLT DKVLHSDNIA QREEYLKLMD SVESNGGKAL
360 370 380
VLSTLHSLGE ELDQLTGIAC ILKYPLPDLD EDDGEE
Length:386
Mass (Da):44,058
Last modified:October 3, 2006 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4E8B7D9BBCD60888
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA34575 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
L11277 Genomic DNA Translation: AAA34575.1 Different initiation.
X77114 Genomic DNA Translation: CAA54375.1
Z71277 Genomic DNA Translation: CAA95860.1
BK006947 Genomic DNA Translation: DAA10542.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S45456

NCBI Reference Sequences

More...
RefSeqi
NP_014397.1, NM_001182840.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YNL001W_mRNA; YNL001W; YNL001W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
855731

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YNL001W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11277 Genomic DNA Translation: AAA34575.1 Different initiation.
X77114 Genomic DNA Translation: CAA54375.1
Z71277 Genomic DNA Translation: CAA95860.1
BK006947 Genomic DNA Translation: DAA10542.1
PIRiS45456
RefSeqiNP_014397.1, NM_001182840.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VGMX-ray2.60A1-386[»]
2VGNX-ray2.50A/B1-386[»]
3IZQelectron microscopy-01-386[»]
3J16electron microscopy-A1-386[»]
5M1Jelectron microscopy3.30A11-381[»]
SMRiP33309
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi35824, 288 interactors
ComplexPortaliCPX-465, DOM34-HBS1 ribosome dissociation complex
DIPiDIP-7520N
IntActiP33309, 15 interactors
MINTiP33309
STRINGi4932.YNL001W

Proteomic databases

MaxQBiP33309
PaxDbiP33309
PRIDEiP33309

Genome annotation databases

EnsemblFungiiYNL001W_mRNA; YNL001W; YNL001W
GeneIDi855731
KEGGisce:YNL001W

Organism-specific databases

EuPathDBiFungiDB:YNL001W
SGDiS000004946, DOM34

Phylogenomic databases

eggNOGiKOG2869, Eukaryota
GeneTreeiENSGT00390000016326
HOGENOMiCLU_023334_3_1_1
InParanoidiP33309
KOiK06965
OMAiFAHERMA

Miscellaneous databases

EvolutionaryTraceiP33309

Protein Ontology

More...
PROi
PR:P33309
RNActiP33309, protein

Family and domain databases

Gene3Di2.30.30.870, 1 hit
3.30.1330.30, 1 hit
3.30.420.60, 1 hit
InterProiView protein in InterPro
IPR042226, eFR1_2_sf
IPR005140, eRF1_1_Pelota
IPR005141, eRF1_2
IPR005142, eRF1_3
IPR029064, L30e-like
IPR038069, Pelota/DOM34_N
IPR004405, Transl-rel_pelota
PANTHERiPTHR10853, PTHR10853, 1 hit
PfamiView protein in Pfam
PF03463, eRF1_1, 1 hit
PF03464, eRF1_2, 1 hit
PF03465, eRF1_3, 1 hit
SMARTiView protein in SMART
SM01194, eRF1_1, 1 hit
SUPFAMiSSF159065, SSF159065, 1 hit
SSF55315, SSF55315, 1 hit
TIGRFAMsiTIGR00111, pelota, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDOM34_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P33309
Secondary accession number(s): D6W1H6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 3, 2006
Last modified: October 7, 2020
This is version 179 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names
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