UniProtKB - P33299 (PRS7_YEAST)
Protein
26S proteasome regulatory subunit 7 homolog
Gene
RPT1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).By similarity
Miscellaneous
Present with 105 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 250 – 257 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATPase activity Source: SGD
- ATP binding Source: UniProtKB-KW
- proteasome-activating ATPase activity Source: InterPro
- TBP-class protein binding Source: GO_Central
- ubiquitin protein ligase binding Source: SGD
GO - Biological processi
- positive regulation of protein catabolic process Source: SGD
- positive regulation of RNA polymerase II transcription preinitiation complex assembly Source: SGD
- proteasome regulatory particle assembly Source: SGD
- ubiquitin-dependent protein catabolic process Source: SGD
Keywordsi
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases R-SCE-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome regulatory subunit 7 homologAlternative name(s): Protein CIM5 Tat-binding homolog 3 |
Gene namesi | Name:RPT1 Synonyms:CIM5, YTA3 Ordered Locus Names:YKL145W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001628, RPT1 |
VEuPathDBi | FungiDB:YKL145W |
Subcellular locationi
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-SubCell
- proteasome regulatory particle, base subcomplex Source: SGD
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084719 | 1 – 467 | 26S proteasome regulatory subunit 7 homologAdd BLAST | 467 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 164 | PhosphoserineCombined sources | 1 | |
Modified residuei | 231 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
The N-terminus is blocked.
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P33299 |
PaxDbi | P33299 |
PRIDEi | P33299 |
2D gel databases
SWISS-2DPAGEi | P33299 |
PTM databases
iPTMneti | P33299 |
Interactioni
Subunit structurei
Interacts with UBR1 and CIC1.
2 PublicationsBinary interactionsi
Hide detailsP33299
GO - Molecular functioni
- TBP-class protein binding Source: GO_Central
- ubiquitin protein ligase binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 33991, 642 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-2883N |
IntActi | P33299, 86 interactors |
MINTi | P33299 |
STRINGi | 4932.YKL145W |
Miscellaneous databases
RNActi | P33299, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the AAA ATPase family.Curated
Phylogenomic databases
eggNOGi | KOG0729, Eukaryota |
GeneTreei | ENSGT01020000230535 |
HOGENOMi | CLU_000688_6_0_1 |
InParanoidi | P33299 |
OMAi | IHANTMN |
Family and domain databases
InterProi | View protein in InterPro IPR005937, 26S_Psome_P45-like IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR027417, P-loop_NTPase IPR035245, PSMC2 |
PANTHERi | PTHR23073:SF13, PTHR23073:SF13, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01242, 26Sp45, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
i Sequence
Sequence statusi: Complete.
P33299-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPPKEDWEKY KAPLEDDDKK PDDDKIVPLT EGDIQVLKSY GAAPYAAKLK
60 70 80 90 100
QTENDLKDIE ARIKEKAGVK ESDTGLAPSH LWDIMGDRQR LGEEHPLQVA
110 120 130 140 150
RCTKIIKGNG ESDETTTDNN NSGNSNSNSN QQSTDADEDD EDAKYVINLK
160 170 180 190 200
QIAKFVVGLG ERVSPTDIEE GMRVGVDRSK YNIELPLPPR IDPSVTMMTV
210 220 230 240 250
EEKPDVTYSD VGGCKDQIEK LREVVELPLL SPERFATLGI DPPKGILLYG
260 270 280 290 300
PPGTGKTLCA RAVANRTDAT FIRVIGSELV QKYVGEGARM VRELFEMART
310 320 330 340 350
KKACIIFFDE IDAVGGARFD DGAGGDNEVQ RTMLELITQL DGFDPRGNIK
360 370 380 390 400
VMFATNRPNT LDPALLRPGR IDRKVEFSLP DLEGRANIFR IHSKSMSVER
410 420 430 440 450
GIRWELISRL CPNSTGAELR SVCTEAGMFA IRARRKVATE KDFLKAVDKV
460
ISGYKKFSST SRYMQYN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z22817 Genomic DNA Translation: CAA80470.1 X73571 Genomic DNA Translation: CAA51973.1 Z28145 Genomic DNA Translation: CAA81986.1 BK006944 Genomic DNA Translation: DAA09017.1 |
PIRi | S34354 |
RefSeqi | NP_012777.1, NM_001179711.1 |
Genome annotation databases
EnsemblFungii | YKL145W_mRNA; YKL145W; YKL145W |
GeneIDi | 853712 |
KEGGi | sce:YKL145W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z22817 Genomic DNA Translation: CAA80470.1 X73571 Genomic DNA Translation: CAA51973.1 Z28145 Genomic DNA Translation: CAA81986.1 BK006944 Genomic DNA Translation: DAA09017.1 |
PIRi | S34354 |
RefSeqi | NP_012777.1, NM_001179711.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3JCO | electron microscopy | 4.80 | H | 1-467 | [»] | |
3JCP | electron microscopy | 4.60 | H | 1-467 | [»] | |
3VLF | X-ray | 3.80 | B/D | 381-467 | [»] | |
4A3V | X-ray | 3.80 | B/D | 378-467 | [»] | |
4CR2 | electron microscopy | 7.70 | H | 1-467 | [»] | |
4CR3 | electron microscopy | 9.30 | H | 1-467 | [»] | |
4CR4 | electron microscopy | 8.80 | H | 1-467 | [»] | |
4JPO | X-ray | 5.00 | C/D | 379-467 | [»] | |
5A5B | electron microscopy | 9.50 | H | 1-467 | [»] | |
5MP9 | electron microscopy | 4.10 | H | 1-467 | [»] | |
5MPA | electron microscopy | 4.50 | H | 1-467 | [»] | |
5MPB | electron microscopy | 7.80 | H | 1-467 | [»] | |
5MPC | electron microscopy | 7.70 | H | 1-467 | [»] | |
5WVI | electron microscopy | 6.30 | H | 1-467 | [»] | |
5WVK | electron microscopy | 4.20 | H | 1-467 | [»] | |
6EF0 | electron microscopy | 4.43 | H | 201-457 | [»] | |
6EF1 | electron microscopy | 4.73 | H | 194-455 | [»] | |
6EF2 | electron microscopy | 4.27 | H | 194-466 | [»] | |
6EF3 | electron microscopy | 4.17 | H | 1-467 | [»] | |
6FVT | electron microscopy | 4.10 | H | 42-467 | [»] | |
6FVU | electron microscopy | 4.50 | H | 42-467 | [»] | |
6FVV | electron microscopy | 5.40 | H | 42-458 | [»] | |
6FVW | electron microscopy | 4.50 | H | 42-467 | [»] | |
6FVX | electron microscopy | 4.90 | H | 42-467 | [»] | |
6FVY | electron microscopy | 6.10 | H | 42-467 | [»] | |
6J2C | electron microscopy | 7.00 | H | 1-467 | [»] | |
6J2N | electron microscopy | 7.50 | H | 1-467 | [»] | |
6J2Q | electron microscopy | 3.80 | H | 1-467 | [»] | |
6J2X | electron microscopy | 3.80 | H | 1-467 | [»] | |
6J30 | electron microscopy | 4.50 | H | 1-467 | [»] | |
SMRi | P33299 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33991, 642 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-2883N |
IntActi | P33299, 86 interactors |
MINTi | P33299 |
STRINGi | 4932.YKL145W |
PTM databases
iPTMneti | P33299 |
2D gel databases
SWISS-2DPAGEi | P33299 |
Proteomic databases
MaxQBi | P33299 |
PaxDbi | P33299 |
PRIDEi | P33299 |
Genome annotation databases
EnsemblFungii | YKL145W_mRNA; YKL145W; YKL145W |
GeneIDi | 853712 |
KEGGi | sce:YKL145W |
Organism-specific databases
SGDi | S000001628, RPT1 |
VEuPathDBi | FungiDB:YKL145W |
Phylogenomic databases
eggNOGi | KOG0729, Eukaryota |
GeneTreei | ENSGT01020000230535 |
HOGENOMi | CLU_000688_6_0_1 |
InParanoidi | P33299 |
OMAi | IHANTMN |
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases R-SCE-6798695, Neutrophil degranulation |
Miscellaneous databases
PROi | PR:P33299 |
RNActi | P33299, protein |
Family and domain databases
InterProi | View protein in InterPro IPR005937, 26S_Psome_P45-like IPR003593, AAA+_ATPase IPR041569, AAA_lid_3 IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR027417, P-loop_NTPase IPR035245, PSMC2 |
PANTHERi | PTHR23073:SF13, PTHR23073:SF13, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF17862, AAA_lid_3, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01242, 26Sp45, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRS7_YEAST | |
Accessioni | P33299Primary (citable) accession number: P33299 Secondary accession number(s): D6VX51 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | April 7, 2021 | |
This is version 197 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families