UniProtKB - P33298 (PRS6B_YEAST)
Protein
26S proteasome regulatory subunit 6B homolog
Gene
RPT3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 213 – 220 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATPase activity Source: SGD
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: IntAct
- proteasome-activating ATPase activity Source: InterPro
GO - Biological processi
- positive regulation of RNA polymerase II transcription preinitiation complex assembly Source: SGD
- proteasome regulatory particle assembly Source: SGD
- ubiquitin-dependent protein catabolic process Source: SGD
Keywordsi
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome regulatory subunit 6B homologAlternative name(s): Protein YNT1 Tat-binding homolog 2 |
Gene namesi | Name:RPT3 Synonyms:YNT1, YTA2 Ordered Locus Names:YDR394W ORF Names:D9509.14 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002802, RPT3 |
VEuPathDBi | FungiDB:YDR394W |
Subcellular locationi
Cytosol
- cytosolic proteasome complex Source: GO_Central
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- proteasome regulatory particle, base subcomplex Source: SGD
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084697 | 1 – 428 | 26S proteasome regulatory subunit 6B homologAdd BLAST | 428 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine1 Publication | 1 | |
Cross-linki | 280 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Post-translational modificationi
N-acetylated by NAT3.1 Publication
Keywords - PTMi
Acetylation, Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P33298 |
PaxDbi | P33298 |
PRIDEi | P33298 |
PTM databases
iPTMneti | P33298 |
Interactioni
Binary interactionsi
Hide detailsP33298
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 32456, 434 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-1587N |
IntActi | P33298, 99 interactors |
MINTi | P33298 |
STRINGi | 4932.YDR394W |
Miscellaneous databases
RNActi | P33298, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P33298 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P33298 |
Family & Domainsi
Sequence similaritiesi
Belongs to the AAA ATPase family.Curated
Phylogenomic databases
eggNOGi | KOG0727, Eukaryota |
GeneTreei | ENSGT00990000203571 |
HOGENOMi | CLU_000688_2_0_1 |
InParanoidi | P33298 |
OMAi | QDIGGMD |
Family and domain databases
InterProi | View protein in InterPro IPR005937, 26S_Psome_P45-like IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR027417, P-loop_NTPase IPR032501, Prot_ATP_ID_OB IPR035256, PSMC4 |
PANTHERi | PTHR23073:SF120, PTHR23073:SF120, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF16450, Prot_ATP_ID_OB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01242, 26Sp45, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
i Sequence
Sequence statusi: Complete.
P33298-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEELGIVTPV EKAVEEKPAV KSYASLLAQL NGTVNNNSAL SNVNSDIYFK
60 70 80 90 100
LKKLEKEYEL LTLQEDYIKD EQRHLKRELK RAQEEVKRIQ SVPLVIGQFL
110 120 130 140 150
EPIDQNTGIV SSTTGMSYVV RILSTLDREL LKPSMSVALH RHSNALVDIL
160 170 180 190 200
PPDSDSSISV MGENEKPDVT YADVGGLDMQ KQEIREAVEL PLVQADLYEQ
210 220 230 240 250
IGIDPPRGVL LYGPPGTGKT MLVKAVANST KAAFIRVNGS EFVHKYLGEG
260 270 280 290 300
PRMVRDVFRL ARENAPSIIF IDEVDSIATK RFDAQTGSDR EVQRILIELL
310 320 330 340 350
TQMDGFDQST NVKVIMATNR ADTLDPALLR PGRLDRKIEF PSLRDRRERR
360 370 380 390 400
LIFGTIASKM SLAPEADLDS LIIRNDSLSG AVIAAIMQEA GLRAVRKNRY
410 420
VILQSDLEEA YATQVKTDNT VDKFDFYK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 342 | S → Y in CAA51972 (PubMed:7754704).Curated | 1 | |
Sequence conflicti | 342 | S → Y in AAA81916 (PubMed:7803857).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73570 Genomic DNA Translation: CAA51972.1 U06229 Genomic DNA Translation: AAA81916.1 U32274 Genomic DNA Translation: AAB64836.1 BK006938 Genomic DNA Translation: DAA12238.1 |
PIRi | S69678 |
RefSeqi | NP_010682.3, NM_001180702.3 |
Genome annotation databases
EnsemblFungii | YDR394W_mRNA; YDR394W; YDR394W |
GeneIDi | 852003 |
KEGGi | sce:YDR394W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73570 Genomic DNA Translation: CAA51972.1 U06229 Genomic DNA Translation: AAA81916.1 U32274 Genomic DNA Translation: AAB64836.1 BK006938 Genomic DNA Translation: DAA12238.1 |
PIRi | S69678 |
RefSeqi | NP_010682.3, NM_001180702.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DZN | X-ray | 2.20 | B/D/F | 348-428 | [»] | |
2DZO | X-ray | 3.00 | B/D | 348-428 | [»] | |
3JCO | electron microscopy | 4.80 | K | 1-428 | [»] | |
3JCP | electron microscopy | 4.60 | K | 1-428 | [»] | |
4CR2 | electron microscopy | 7.70 | K | 1-428 | [»] | |
4CR3 | electron microscopy | 9.30 | K | 1-428 | [»] | |
4CR4 | electron microscopy | 8.80 | K | 1-428 | [»] | |
5A5B | electron microscopy | 9.50 | K | 1-428 | [»] | |
5MP9 | electron microscopy | 4.10 | K | 1-428 | [»] | |
5MPA | electron microscopy | 4.50 | K | 1-428 | [»] | |
5MPB | electron microscopy | 7.80 | K | 1-428 | [»] | |
5MPC | electron microscopy | 7.70 | K | 1-428 | [»] | |
5WVI | electron microscopy | 6.30 | K | 1-428 | [»] | |
5WVK | electron microscopy | 4.20 | K | 1-428 | [»] | |
6EF0 | electron microscopy | 4.43 | K | 157-428 | [»] | |
6EF1 | electron microscopy | 4.73 | K | 153-428 | [»] | |
6EF2 | electron microscopy | 4.27 | K | 170-428 | [»] | |
6EF3 | electron microscopy | 4.17 | K | 1-428 | [»] | |
6FVT | electron microscopy | 4.10 | K | 35-428 | [»] | |
6FVU | electron microscopy | 4.50 | K | 35-428 | [»] | |
6FVV | electron microscopy | 5.40 | K | 35-428 | [»] | |
6FVW | electron microscopy | 4.50 | K | 45-428 | [»] | |
6FVX | electron microscopy | 4.90 | K | 35-428 | [»] | |
6FVY | electron microscopy | 6.10 | K | 35-428 | [»] | |
6J2C | electron microscopy | 7.00 | K | 1-428 | [»] | |
6J2N | electron microscopy | 7.50 | K | 1-428 | [»] | |
6J2Q | electron microscopy | 3.80 | K | 1-428 | [»] | |
6J2X | electron microscopy | 3.80 | K | 1-428 | [»] | |
6J30 | electron microscopy | 4.50 | K | 1-428 | [»] | |
SMRi | P33298 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32456, 434 interactors |
ComplexPortali | CPX-2262, 26S Proteasome complex |
DIPi | DIP-1587N |
IntActi | P33298, 99 interactors |
MINTi | P33298 |
STRINGi | 4932.YDR394W |
PTM databases
iPTMneti | P33298 |
Proteomic databases
MaxQBi | P33298 |
PaxDbi | P33298 |
PRIDEi | P33298 |
Genome annotation databases
EnsemblFungii | YDR394W_mRNA; YDR394W; YDR394W |
GeneIDi | 852003 |
KEGGi | sce:YDR394W |
Organism-specific databases
SGDi | S000002802, RPT3 |
VEuPathDBi | FungiDB:YDR394W |
Phylogenomic databases
eggNOGi | KOG0727, Eukaryota |
GeneTreei | ENSGT00990000203571 |
HOGENOMi | CLU_000688_2_0_1 |
InParanoidi | P33298 |
OMAi | QDIGGMD |
Enzyme and pathway databases
Reactomei | R-SCE-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-SCE-5668541, TNFR2 non-canonical NF-kB pathway R-SCE-5689880, Ub-specific processing proteases |
Miscellaneous databases
EvolutionaryTracei | P33298 |
PROi | PR:P33298 |
RNActi | P33298, protein |
Family and domain databases
InterProi | View protein in InterPro IPR005937, 26S_Psome_P45-like IPR003593, AAA+_ATPase IPR003959, ATPase_AAA_core IPR003960, ATPase_AAA_CS IPR027417, P-loop_NTPase IPR032501, Prot_ATP_ID_OB IPR035256, PSMC4 |
PANTHERi | PTHR23073:SF120, PTHR23073:SF120, 1 hit |
Pfami | View protein in Pfam PF00004, AAA, 1 hit PF16450, Prot_ATP_ID_OB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01242, 26Sp45, 1 hit |
PROSITEi | View protein in PROSITE PS00674, AAA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRS6B_YEAST | |
Accessioni | P33298Primary (citable) accession number: P33298 Secondary accession number(s): D6VT28 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | October 5, 2010 | |
Last modified: | February 10, 2021 | |
This is version 195 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families