UniProtKB - P33242 (STF1_MOUSE)
Protein
Steroidogenic factor 1
Gene
Nr5a1
Organism
Mus musculus (Mouse)
Status
Functioni
Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity (By similarity). Transcription repressor of the Moloney leukemia virus long terminal repeat in undifferentiated murine embryonal carcinoma cells. Binds phosphatidylcholine and phospholipids with a phosphatidylinositol (PI) headgroup, in particular phosphatidyl(3,4)bisphosphate, phosphatidyl(3,5)bisphosphate and phosphatidyl(3,4,5)triphosphate. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.By similarity2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 342 | Phosphatidylcholine; via amide nitrogen1 Publication | 1 | |
Binding sitei | 437 | Phosphatidylcholine1 Publication | 1 | |
Binding sitei | 441 | Phosphatidylcholine1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 10 – 85 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 76 | |
Zinc fingeri | 13 – 33 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 49 – 73 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- chromatin binding Source: BHF-UCL
- DNA binding Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- double-stranded DNA binding Source: MGI
- enzyme binding Source: MGI
- nuclear receptor activity Source: MGI
- phospholipid binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: BHF-UCL
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- adrenal gland development Source: MGI
- calcineurin-mediated signaling Source: MGI
- cell differentiation Source: MGI
- female gonad development Source: UniProtKB
- hormone-mediated signaling pathway Source: MGI
- hormone metabolic process Source: MGI
- luteinization Source: MGI
- maintenance of protein location in nucleus Source: MGI
- male gonad development Source: MGI
- male sex determination Source: MGI
- multicellular organism aging Source: MGI
- negative regulation of female gonad development Source: UniProtKB
- positive regulation of gene expression Source: MGI
- positive regulation of male gonad development Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- reproductive process Source: MGI
- response to gonadotropin-releasing hormone Source: Ensembl
- sex determination Source: UniProtKB
- tissue development Source: MGI
- transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding, Receptor, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Lipid-binding, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway R-MMU-4090294, SUMOylation of intracellular receptors |
Chemistry databases
SwissLipidsi | SLP:000001543 |
Names & Taxonomyi
Protein namesi | Recommended name: Steroidogenic factor 1Short name: SF-1 Short name: STF-1 Alternative name(s): Adrenal 4-binding protein Embryonal LTR-binding protein Short name: ELP Embryonal long terminal repeat-binding protein Fushi tarazu factor homolog 1 Nuclear receptor subfamily 5 group A member 1 Steroid hormone receptor Ad4BP Steroid hydroxylase positive regulator |
Gene namesi | Name:Nr5a1 Synonyms:Ftzf1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1346833, Nr5a1 |
Subcellular locationi
Nucleus
Nucleus
- nucleus Source: HGNC-UCL
Other locations
- RNA polymerase II transcription regulator complex Source: BHF-UCL
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 270 | A → W: Reduced lipid binding and transactivation. Abolishes ligand binding and reduces transactivation; when associated with F-345. 2 Publications | 1 | |
Mutagenesisi | 314 | R → M: Strongly reduced transactivation. 1 Publication | 1 | |
Mutagenesisi | 345 | L → F: Abolishes ligand binding and reduces transactivation; when associated with W-270. 2 Publications | 1 | |
Mutagenesisi | 345 | L → W: Strongly reduced transactivation. 2 Publications | 1 | |
Mutagenesisi | 348 | L → W: Strongly reduced transactivation. 1 Publication | 1 | |
Mutagenesisi | 349 | V → W: Strongly reduced transactivation. 1 Publication | 1 | |
Mutagenesisi | 352 | A → W: Strongly reduced transactivation. 1 Publication | 1 | |
Mutagenesisi | 434 | A → W: Strongly reduced transactivation. 1 Publication | 1 | |
Mutagenesisi | 440 | H → D: Strongly reduced transactivation; when associated with D-442. | 1 | |
Mutagenesisi | 441 | K → E: Strongly reduced transactivation. 1 Publication | 1 | |
Mutagenesisi | 442 | H → D: Strongly reduced transactivation; when associated with D-440. | 1 |
Chemistry databases
ChEMBLi | CHEMBL1764943 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053732 | 1 – 462 | Steroidogenic factor 1Add BLAST | 462 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 34 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 38 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 72 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 119 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 194 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 203 | Phosphoserine; by CDK7By similarity | 1 |
Post-translational modificationi
Acetylation stimulates the transcriptional activity.By similarity
Sumoylation reduces CDK7-mediated phosphorylation on Ser-203.By similarity
Phosphorylated on Ser-203 by CDK7. This phosphorylation promotes transcriptional activity (By similarity).By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P33242 |
PRIDEi | P33242 |
PTM databases
iPTMneti | P33242 |
PhosphoSitePlusi | P33242 |
Expressioni
Developmental stagei
Expressed during early embryonic development. Expressed only in undifferentiated embryonal carcinoma cells.
Gene expression databases
Bgeei | ENSMUSG00000026751, Expressed in cerebral cortex and 132 other tissues |
Genevisiblei | P33242, MM |
Interactioni
Subunit structurei
Binds DNA as a monomer (By similarity).
Part of a complex consisting of SFPQ, NONO and NR5A1.
Interacts with DGKQ and CDK7 (By similarity).
Interacts with NR0B2, NCOA2 and PPARGC1A. Binds to and activated by HIPK3.
By similarity5 PublicationsGO - Molecular functioni
- enzyme binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204975, 16 interactors |
ELMi | P33242 |
IntActi | P33242, 1 interactor |
STRINGi | 10090.ENSMUSP00000108504 |
Miscellaneous databases
RNActi | P33242, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P33242 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P33242 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 223 – 460 | NR LBDPROSITE-ProRule annotationAdd BLAST | 238 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 13 – 33 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 49 – 73 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG4218, Eukaryota |
GeneTreei | ENSGT00940000153391 |
HOGENOMi | CLU_011437_0_0_1 |
InParanoidi | P33242 |
OMAi | KAECPDY |
OrthoDBi | 619653at2759 |
TreeFami | TF350737 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
IDEALi | IID50074 |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR016355, NR5_fam IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR24086, PTHR24086, 1 hit |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PIRSFi | PIRSF002530, Nuc_orph_FTZ-F1, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: P33242-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDYSYDEDLD ELCPVCGDKV SGYHYGLLTC ESCKGFFKRT VQNNKHYTCT
60 70 80 90 100
ESQSCKIDKT QRKRCPFCRF QKCLTVGMRL EAVRADRMRG GRNKFGPMYK
110 120 130 140 150
RDRALKQQKK AQIRANGFKL ETGPPMGVPP PPPPPPDYML PPSLHAPEPK
160 170 180 190 200
ALVSGPPSGP LGDFGAPSLP MAVPGPHGPL AGYLYPAFSN RTIKSEYPEP
210 220 230 240 250
YASPPQQPGP PYSYPEPFSG GPNVPELILQ LLQLEPEEDQ VRARIVGCLQ
260 270 280 290 300
EPAKSRSDQP APFSLLCRMA DQTFISIVDW ARRCMVFKEL EVADQMTLLQ
310 320 330 340 350
NCWSELLVLD HIYRQVQYGK EDSILLVTGQ EVELSTVAVQ AGSLLHSLVL
360 370 380 390 400
RAQELVLQLH ALQLDRQEFV CLKFLILFSL DVKFLNNHSL VKDAQEKANA
410 420 430 440 450
ALLDYTLCHY PHCGDKFQQL LLCLVEVRAL SMQAKEYLYH KHLGNEMPRN
460
NLLIEMLQAK QT
Sequence cautioni
The sequence BAA01441 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 164 | F → I in BAA01441 (PubMed:1545809).Curated | 1 | |
Sequence conflicti | 256 | R → G in BAA01441 (PubMed:1545809).Curated | 1 | |
Sequence conflicti | 328 | T → S in BAA01441 (PubMed:1545809).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 172 | A → S in strain: C3H/HeJ and DBA/2J; does not change transcriptional activity. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003715 | 333 – 462 | ELSTV…QAKQT → TELVKPLVLHNPRPLRADSG HPKFQIQGHALARLLCVLGP FEEPQCGMVSGSSYRR in isoform 2. 1 PublicationAdd BLAST | 130 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10584 mRNA Translation: BAA01441.1 Different initiation. S65878 , S65875, S65919, S65876, S65877 Genomic DNA Translation: AAB28338.1 S65876, S65875, S65919 Genomic DNA Translation: AAB28339.1 AF511594 mRNA Translation: AAM43952.1 AK134378 mRNA Translation: BAE22121.1 AK144376 mRNA Translation: BAE25855.1 AL844842 Genomic DNA No translation available. BC110476 mRNA Translation: AAI10477.1 BC110477 mRNA Translation: AAI10478.1 |
CCDSi | CCDS16011.1 [P33242-1] |
PIRi | A40716 A42128 |
RefSeqi | NP_001303616.1, NM_001316687.1 [P33242-1] NP_620639.1, NM_139051.3 [P33242-1] |
Genome annotation databases
Ensembli | ENSMUST00000028084; ENSMUSP00000028084; ENSMUSG00000026751 [P33242-1] ENSMUST00000112883; ENSMUSP00000108504; ENSMUSG00000026751 [P33242-1] |
GeneIDi | 26423 |
KEGGi | mmu:26423 |
UCSCi | uc008jnr.1, mouse [P33242-1] uc008jnt.1, mouse [P33242-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10584 mRNA Translation: BAA01441.1 Different initiation. S65878 , S65875, S65919, S65876, S65877 Genomic DNA Translation: AAB28338.1 S65876, S65875, S65919 Genomic DNA Translation: AAB28339.1 AF511594 mRNA Translation: AAM43952.1 AK134378 mRNA Translation: BAE22121.1 AK144376 mRNA Translation: BAE25855.1 AL844842 Genomic DNA No translation available. BC110476 mRNA Translation: AAI10477.1 BC110477 mRNA Translation: AAI10478.1 |
CCDSi | CCDS16011.1 [P33242-1] |
PIRi | A40716 A42128 |
RefSeqi | NP_001303616.1, NM_001316687.1 [P33242-1] NP_620639.1, NM_139051.3 [P33242-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YMT | X-ray | 1.20 | A | 219-462 | [»] | |
1YP0 | X-ray | 1.50 | A | 223-461 | [»] | |
2FF0 | NMR | - | A | 10-111 | [»] | |
3F7D | X-ray | 2.20 | A | 219-462 | [»] | |
SMRi | P33242 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 204975, 16 interactors |
ELMi | P33242 |
IntActi | P33242, 1 interactor |
STRINGi | 10090.ENSMUSP00000108504 |
Chemistry databases
ChEMBLi | CHEMBL1764943 |
SwissLipidsi | SLP:000001543 |
PTM databases
iPTMneti | P33242 |
PhosphoSitePlusi | P33242 |
Proteomic databases
PaxDbi | P33242 |
PRIDEi | P33242 |
Protocols and materials databases
Antibodypediai | 16333, 411 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028084; ENSMUSP00000028084; ENSMUSG00000026751 [P33242-1] ENSMUST00000112883; ENSMUSP00000108504; ENSMUSG00000026751 [P33242-1] |
GeneIDi | 26423 |
KEGGi | mmu:26423 |
UCSCi | uc008jnr.1, mouse [P33242-1] uc008jnt.1, mouse [P33242-2] |
Organism-specific databases
CTDi | 2516 |
MGIi | MGI:1346833, Nr5a1 |
Phylogenomic databases
eggNOGi | KOG4218, Eukaryota |
GeneTreei | ENSGT00940000153391 |
HOGENOMi | CLU_011437_0_0_1 |
InParanoidi | P33242 |
OMAi | KAECPDY |
OrthoDBi | 619653at2759 |
TreeFami | TF350737 |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway R-MMU-4090294, SUMOylation of intracellular receptors |
Miscellaneous databases
BioGRID-ORCSi | 26423, 0 hits in 17 CRISPR screens |
EvolutionaryTracei | P33242 |
PROi | PR:P33242 |
RNActi | P33242, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026751, Expressed in cerebral cortex and 132 other tissues |
Genevisiblei | P33242, MM |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
IDEALi | IID50074 |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR016355, NR5_fam IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
PANTHERi | PTHR24086, PTHR24086, 1 hit |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PIRSFi | PIRSF002530, Nuc_orph_FTZ-F1, 1 hit |
PRINTSi | PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | STF1_MOUSE | |
Accessioni | P33242Primary (citable) accession number: P33242 Secondary accession number(s): Q812G5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 187 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families