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UniProtKB - P33242 (STF1_MOUSE)

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Protein

Steroidogenic factor 1

Gene

Nr5a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity (By similarity). Transcription repressor of the Moloney leukemia virus long terminal repeat in undifferentiated murine embryonal carcinoma cells. Binds phosphatidylcholine and phospholipids with a phosphatidylinositol (PI) headgroup, in particular phosphatidyl(3,4)bisphosphate, phosphatidyl(3,5)bisphosphate and phosphatidyl(3,4,5)triphosphate. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei342Lipid headgroup; via amide nitrogen1 Publication1
Binding sitei437Lipid headgroup1 Publication1
Binding sitei441Lipid headgroup1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi10 – 85Nuclear receptorPROSITE-ProRule annotationAdd BLAST76
Zinc fingeri13 – 33NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri49 – 73NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

  • adrenal gland development Source: MGI
  • calcineurin-mediated signaling Source: MGI
  • cell differentiation Source: MGI
  • female gonad development Source: UniProtKB
  • hormone-mediated signaling pathway Source: MGI
  • hormone metabolic process Source: MGI
  • luteinization Source: MGI
  • maintenance of protein location in nucleus Source: MGI
  • male gonad development Source: MGI
  • multicellular organism aging Source: MGI
  • negative regulation of female gonad development Source: UniProtKB
  • positive regulation of gene expression Source: MGI
  • positive regulation of male gonad development Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI
  • reproductive process Source: MGI
  • response to gonadotropin-releasing hormone Source: Ensembl
  • sex determination Source: UniProtKB
  • tissue development Source: MGI
  • transcription by RNA polymerase II Source: MGI
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionActivator, DNA-binding, Receptor, Repressor
Biological processTranscription, Transcription regulation
LigandLipid-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-383280 Nuclear Receptor transcription pathway

Chemistry databases

SwissLipidsiSLP:000001543

Names & Taxonomyi

Protein namesi
Recommended name:
Steroidogenic factor 1
Short name:
SF-1
Short name:
STF-1
Alternative name(s):
Adrenal 4-binding protein
Embryonal LTR-binding protein
Short name:
ELP
Embryonal long terminal repeat-binding protein
Fushi tarazu factor homolog 1
Nuclear receptor subfamily 5 group A member 1
Steroid hormone receptor Ad4BP
Steroid hydroxylase positive regulator
Gene namesi
Name:Nr5a1
Synonyms:Ftzf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1346833 Nr5a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi270A → W: Reduced lipid binding and transactivation. Abolishes ligand binding and reduces transactivation; when associated with F-345. 2 Publications1
Mutagenesisi314R → M: Strongly reduced transactivation. 1 Publication1
Mutagenesisi345L → F: Abolishes ligand binding and reduces transactivation; when associated with W-270. 2 Publications1
Mutagenesisi345L → W: Strongly reduced transactivation. 2 Publications1
Mutagenesisi348L → W: Strongly reduced transactivation. 1 Publication1
Mutagenesisi349V → W: Strongly reduced transactivation. 1 Publication1
Mutagenesisi352A → W: Strongly reduced transactivation. 1 Publication1
Mutagenesisi434A → W: Strongly reduced transactivation. 1 Publication1
Mutagenesisi440H → D: Strongly reduced transactivation; when associated with D-442. 1
Mutagenesisi441K → E: Strongly reduced transactivation. 1 Publication1
Mutagenesisi442H → D: Strongly reduced transactivation; when associated with D-440. 1

Chemistry databases

ChEMBLiCHEMBL1764943

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000537321 – 462Steroidogenic factor 1Add BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34N6-acetyllysineBy similarity1
Modified residuei38N6-acetyllysineBy similarity1
Modified residuei72N6-acetyllysineBy similarity1
Cross-linki119Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki194Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei203Phosphoserine; by CDK7By similarity1

Post-translational modificationi

Acetylation stimulates the transcriptional activity.By similarity
Sumoylation reduces CDK7-mediated phosphorylation on Ser-203.By similarity
Phosphorylated on Ser-203 by CDK7. This phosphorylation promotes transcriptional activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP33242
PRIDEiP33242

PTM databases

iPTMnetiP33242
PhosphoSitePlusiP33242

Expressioni

Developmental stagei

Expressed during early embryonic development. Expressed only in undifferentiated embryonal carcinoma cells.

Gene expression databases

BgeeiENSMUSG00000026751
CleanExiMM_NR5A1
GenevisibleiP33242 MM

Interactioni

Subunit structurei

Binds DNA as a monomer (By similarity). Part of a complex consisting of SFPQ, NONO and NR5A1. Interacts with DGKQ and CDK7 (By similarity). Interacts with NR0B2, NCOA2 and PPARGC1A. Binds to and activated by HIPK3.By similarity5 Publications

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi204975, 16 interactors
ELMiP33242
IntActiP33242, 1 interactor
STRINGi10090.ENSMUSP00000028084

Structurei

Secondary structure

1462
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 16Combined sources3
Beta strandi22 – 24Combined sources3
Beta strandi27 – 29Combined sources3
Helixi31 – 41Combined sources11
Turni42 – 44Combined sources3
Helixi61 – 64Combined sources4
Helixi66 – 76Combined sources11
Helixi80 – 82Combined sources3
Turni94 – 97Combined sources4
Helixi98 – 106Combined sources9
Beta strandi220 – 222Combined sources3
Helixi226 – 234Combined sources9
Helixi238 – 249Combined sources12
Beta strandi252 – 254Combined sources3
Turni255 – 257Combined sources3
Helixi263 – 283Combined sources21
Helixi287 – 289Combined sources3
Helixi292 – 318Combined sources27
Beta strandi323 – 325Combined sources3
Beta strandi331 – 333Combined sources3
Helixi334 – 340Combined sources7
Helixi343 – 361Combined sources19
Helixi366 – 377Combined sources12
Helixi382 – 384Combined sources3
Helixi388 – 409Combined sources22
Turni411 – 413Combined sources3
Helixi416 – 443Combined sources28
Helixi452 – 458Combined sources7

3D structure databases

ProteinModelPortaliP33242
SMRiP33242
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33242

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini223 – 460NR LBDPROSITE-ProRule annotationAdd BLAST238

Sequence similaritiesi

Belongs to the nuclear hormone receptor family. NR5 subfamily.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri13 – 33NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri49 – 73NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4218 Eukaryota
ENOG410YWC2 LUCA
GeneTreeiENSGT00870000136388
HOVERGENiHBG106677
InParanoidiP33242
KOiK08560
OMAiLQEPAKG
OrthoDBiEOG091G06RK
TreeFamiTF350737

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR035500 NHR_like_dom_sf
IPR016355 NR5_fam
IPR000536 Nucl_hrmn_rcpt_lig-bd
IPR001723 Nuclear_hrmn_rcpt
IPR001628 Znf_hrmn_rcpt
IPR013088 Znf_NHR/GATA
PANTHERiPTHR24086 PTHR24086, 1 hit
PfamiView protein in Pfam
PF00104 Hormone_recep, 1 hit
PF00105 zf-C4, 1 hit
PIRSFiPIRSF002530 Nuc_orph_FTZ-F1, 1 hit
PRINTSiPR00398 STRDHORMONER
PR00047 STROIDFINGER
SMARTiView protein in SMART
SM00430 HOLI, 1 hit
SM00399 ZnF_C4, 1 hit
SUPFAMiSSF48508 SSF48508, 2 hits
PROSITEiView protein in PROSITE
PS51843 NR_LBD, 1 hit
PS00031 NUCLEAR_REC_DBD_1, 1 hit
PS51030 NUCLEAR_REC_DBD_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P33242-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDYSYDEDLD ELCPVCGDKV SGYHYGLLTC ESCKGFFKRT VQNNKHYTCT 
        60         70         80         90        100
ESQSCKIDKT QRKRCPFCRF QKCLTVGMRL EAVRADRMRG GRNKFGPMYK 
       110        120        130        140        150
RDRALKQQKK AQIRANGFKL ETGPPMGVPP PPPPPPDYML PPSLHAPEPK 
       160        170        180        190        200
ALVSGPPSGP LGDFGAPSLP MAVPGPHGPL AGYLYPAFSN RTIKSEYPEP 
       210        220        230        240        250
YASPPQQPGP PYSYPEPFSG GPNVPELILQ LLQLEPEEDQ VRARIVGCLQ 
       260        270        280        290        300
EPAKSRSDQP APFSLLCRMA DQTFISIVDW ARRCMVFKEL EVADQMTLLQ 
       310        320        330        340        350
NCWSELLVLD HIYRQVQYGK EDSILLVTGQ EVELSTVAVQ AGSLLHSLVL 
       360        370        380        390        400
RAQELVLQLH ALQLDRQEFV CLKFLILFSL DVKFLNNHSL VKDAQEKANA 
       410        420        430        440        450
ALLDYTLCHY PHCGDKFQQL LLCLVEVRAL SMQAKEYLYH KHLGNEMPRN 
       460 
NLLIEMLQAK QT
Length:462
Mass (Da):52,077
Last modified:July 27, 2011 - v3
Checksum:i67045B563403CF5A
GO
Isoform 2 (identifier: P33242-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     333-462: ELSTVAVQAG...LIEMLQAKQT → TELVKPLVLH...GMVSGSSYRR

Show »
Length:388
Mass (Da):43,387
Checksum:iE9A38FD8427F5888
GO

Sequence cautioni

The sequence BAA01441 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164F → I in BAA01441 (PubMed:1545809).Curated1
Sequence conflicti256R → G in BAA01441 (PubMed:1545809).Curated1
Sequence conflicti328T → S in BAA01441 (PubMed:1545809).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti172A → S in strain: C3H/HeJ and DBA/2J; does not change transcriptional activity. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003715333 – 462ELSTV…QAKQT → TELVKPLVLHNPRPLRADSG HPKFQIQGHALARLLCVLGP FEEPQCGMVSGSSYRR in isoform 2. 1 PublicationAdd BLAST130

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10584 mRNA Translation: BAA01441.1 Different initiation.
S65878
, S65875, S65919, S65876, S65877 Genomic DNA Translation: AAB28338.1
S65876, S65875, S65919 Genomic DNA Translation: AAB28339.1
AF511594 mRNA Translation: AAM43952.1
AK134378 mRNA Translation: BAE22121.1
AK144376 mRNA Translation: BAE25855.1
AL844842 Genomic DNA Translation: CAM21930.1
BC110476 mRNA Translation: AAI10477.1
BC110477 mRNA Translation: AAI10478.1
CCDSiCCDS16011.1 [P33242-1]
PIRiA40716
A42128
RefSeqiNP_001303616.1, NM_001316687.1 [P33242-1]
NP_620639.1, NM_139051.3 [P33242-1]
UniGeneiMm.31387

Genome annotation databases

EnsembliENSMUST00000028084; ENSMUSP00000028084; ENSMUSG00000026751 [P33242-1]
ENSMUST00000112883; ENSMUSP00000108504; ENSMUSG00000026751 [P33242-1]
GeneIDi26423
KEGGimmu:26423
UCSCiuc008jnr.1 mouse [P33242-1]
uc008jnt.1 mouse [P33242-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSTF1_MOUSE
AccessioniPrimary (citable) accession number: P33242
Secondary accession number(s): Q812G5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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