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UniProtKB - P33229 (RALR_ECOLI)
Protein
Endodeoxyribonuclease toxin RalR
Gene
ralR
Organism
Escherichia coli (strain K12)
Status
Functioni
Toxic component of a type I toxin-antitoxin (TA) system. Upon overexpression inhibits growth and reduces colony-forming units in both the presence and absence of the Rac prophage, cells become filamentous. Has deoxyribonuclease activity (probably endonucleolytic), does not digest RNA. Its toxic effects are neutralized by sRNA antitoxin RalA, which is encoded in trans on the opposite DNA strand (PubMed:24748661).
Has RAL-like activity (PubMed:7476171).
2 PublicationsMiscellaneous
This protein is encoded on the Rac prophage.1 Publication
Cofactori
Protein has several cofactor binding sites:Activity regulationi
Inhibited by EDTA.1 Publication
GO - Molecular functioni
- double-stranded DNA endodeoxyribonuclease activity Source: EcoCyc
GO - Biological processi
- DNA restriction-modification system Source: UniProtKB-KW
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | Antibiotic resistance, Restriction system, Toxin-antitoxin system |
Ligand | Calcium, Magnesium |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11900-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Endodeoxyribonuclease toxin RalR1 Publication (EC:3.1.-.-)Short name: DNase RalR1 Publication Alternative name(s): Restriction alleviation and modification enhancement protein1 Publication Toxin RalR1 Publication |
Gene namesi | Name:ralR Synonyms:lar1 Publication, ral, ydaB Ordered Locus Names:b1348, JW5208 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
The single ralR and double ralR-ralA mutant have increased sensitivity to the antibiotic fosfomycin.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 43 – 46 | EKEA → AIAE: No longer inhibits growth. 1 Publication | 4 | |
Mutagenesisi | 52 | K → E: No longer inhibits growth, has no DNase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000084360 | 1 – 64 | Endodeoxyribonuclease toxin RalRAdd BLAST | 64 |
Proteomic databases
PaxDbi | P33229 |
PRIDEi | P33229 |
Expressioni
Inductioni
The sRNA antitoxin RalA probably works by inhibiting translation of the RalR mRNA. The ability of RalA to block the toxicity of RalR relies on a 16-nt sequence which is complementary to a section of the RalR coding region; in the presence of RalA sRNA decreased amounts of RalR protein accumulate with no significant changes in the level of RalR RNA.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261586, 121 interactors |
STRINGi | 511145.b1348 |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG50349DV, Bacteria |
HOGENOMi | CLU_207096_0_0_6 |
OMAi | KIMRYDN |
Family and domain databases
InterProi | View protein in InterPro IPR019908, Toxin_RalR |
TIGRFAMsi | TIGR03655, anti_R_Lar, 1 hit |
i Sequence
Sequence statusi: Complete.
P33229-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRYDNVKPCP FCGCPSVTVK AISGYYRAKC NGCESRTGYG GSEKEALERW
60
NKRTTGNNNG GVHV
Sequence cautioni
The sequence AAA16180 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L23927 Unassigned DNA Translation: AAA16180.1 Different initiation. U00096 Genomic DNA Translation: AAC74430.1 AP009048 Genomic DNA Translation: BAA14948.1 |
PIRi | G64884 |
RefSeqi | NP_415864.1, NC_000913.3 WP_001317028.1, NZ_SSUR01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC74430; AAC74430; b1348 BAA14948; BAA14948; BAA14948 |
GeneIDi | 66674824 945914 |
KEGGi | ecj:JW5208 eco:b1348 |
PATRICi | fig|83333.113.peg.1370 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L23927 Unassigned DNA Translation: AAA16180.1 Different initiation. U00096 Genomic DNA Translation: AAC74430.1 AP009048 Genomic DNA Translation: BAA14948.1 |
PIRi | G64884 |
RefSeqi | NP_415864.1, NC_000913.3 WP_001317028.1, NZ_SSUR01000011.1 |
3D structure databases
AlphaFoldDBi | P33229 |
SMRi | P33229 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261586, 121 interactors |
STRINGi | 511145.b1348 |
Proteomic databases
PaxDbi | P33229 |
PRIDEi | P33229 |
Genome annotation databases
EnsemblBacteriai | AAC74430; AAC74430; b1348 BAA14948; BAA14948; BAA14948 |
GeneIDi | 66674824 945914 |
KEGGi | ecj:JW5208 eco:b1348 |
PATRICi | fig|83333.113.peg.1370 |
Organism-specific databases
EchoBASEi | EB1845 |
Phylogenomic databases
eggNOGi | ENOG50349DV, Bacteria |
HOGENOMi | CLU_207096_0_0_6 |
OMAi | KIMRYDN |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11900-MONOMER |
Miscellaneous databases
PROi | PR:P33229 |
Family and domain databases
InterProi | View protein in InterPro IPR019908, Toxin_RalR |
TIGRFAMsi | TIGR03655, anti_R_Lar, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RALR_ECOLI | |
Accessioni | P33229Primary (citable) accession number: P33229 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 127 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |