UniProtKB - P33226 (TORC_ECOLI)
Protein
Cytochrome c-type protein TorC
Gene
torC
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase TorA. Acts by transferring electrons from the membranous menaquinones to TorA. This transfer probably involves an electron transfer pathway from menaquinones to the N-terminal domain of TorC, then from the N-terminus to the C-terminus, and finally to TorA. TorC apocytochrome negatively autoregulates the torCAD operon probably by inhibiting the TorS kinase activity.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 48 | Heme 1; covalentBy similarity | 1 | |
Binding sitei | 51 | Heme 1; covalentBy similarity | 1 | |
Metal bindingi | 52 | Iron (heme 1 axial ligand)By similarity | 1 | |
Binding sitei | 77 | Heme 2; covalentBy similarity | 1 | |
Binding sitei | 80 | Heme 2; covalentBy similarity | 1 | |
Metal bindingi | 81 | Iron (heme 2 axial ligand)By similarity | 1 | |
Binding sitei | 138 | Heme 3; covalentBy similarity | 1 | |
Binding sitei | 141 | Heme 3; covalentBy similarity | 1 | |
Metal bindingi | 142 | Iron (heme 3 axial ligand)By similarity | 1 | |
Binding sitei | 170 | Heme 4; covalentBy similarity | 1 | |
Binding sitei | 173 | Heme 4; covalentBy similarity | 1 | |
Metal bindingi | 174 | Iron (heme 4 axial ligand)By similarity | 1 | |
Binding sitei | 329 | Heme 5; covalentBy similarity | 1 | |
Binding sitei | 332 | Heme 5; covalentBy similarity | 1 | |
Metal bindingi | 333 | Iron (heme 5 axial ligand)By similarity | 1 |
GO - Molecular functioni
- electron transfer activity Source: EcoCyc
- heme binding Source: EcoCyc
- iron ion binding Source: InterPro
GO - Biological processi
- aerobic respiration Source: EcoCyc
- anaerobic electron transport chain Source: EcoCyc
- anaerobic respiration Source: EcoCyc
- negative regulation of signal transduction Source: EcoCyc
Keywordsi
Biological process | Electron transport, Transport |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11815-MONOMER MetaCyc:EG11815-MONOMER |
Protein family/group databases
TCDBi | 5.A.3.4.1, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome c-type protein TorC |
Gene namesi | Name:torC Ordered Locus Names:b0996, JW0981 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Cell Wall
- Gram-negative-bacterium-type cell wall Source: InterPro
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: EcoCyc
- membrane Source: EcoCyc
- outer membrane-bounded periplasmic space Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 17 – 37 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 38 – 390 | PeriplasmicSequence analysisAdd BLAST | 353 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 329 | C → S: Decrease in expression of the torCAD operon. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000108427 | 1 – 390 | Cytochrome c-type protein TorCAdd BLAST | 390 |
Post-translational modificationi
Binds 5 heme groups per subunit.By similarity
Proteomic databases
PaxDbi | P33226 |
PRIDEi | P33226 |
Interactioni
Subunit structurei
The N-terminal domain interacts with TorA. The immature C-terminal domain can bind to the N-terminal detector region of TorS.
Binary interactionsi
P33226
With | #Exp. | IntAct |
---|---|---|
torA [P33225] | 3 | EBI-553710,EBI-557008 |
Protein-protein interaction databases
BioGRIDi | 4261495, 10 interactors |
ComplexPortali | CPX-319, Trimethylamine-N-oxide reductase TorAC complex |
DIPi | DIP-11014N |
IntActi | P33226, 2 interactors |
STRINGi | 511145.b0996 |
Family & Domainsi
Sequence similaritiesi
Belongs to the TorC/TorY family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3005, Bacteria |
HOGENOMi | CLU_058814_0_0_6 |
Family and domain databases
Gene3Di | 1.10.3820.10, 1 hit |
InterProi | View protein in InterPro IPR009154, Membr-bd_4haem_cyt_TorC IPR011031, Multihaem_cyt IPR036280, Multihaem_cyt_sf IPR005126, NapC/NirT_cyt_c_N IPR038266, NapC/NirT_cytc_sf |
Pfami | View protein in Pfam PF03264, Cytochrom_NNT, 1 hit |
PIRSFi | PIRSF000014, 4_hem_cytch_TorC, 1 hit |
SUPFAMi | SSF48695, SSF48695, 1 hit |
TIGRFAMsi | TIGR02162, torC, 1 hit |
PROSITEi | View protein in PROSITE PS51008, MULTIHEME_CYTC, 1 hit |
i Sequence
Sequence statusi: Complete.
P33226-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRKLWNALRR PSARWSVLAL VAIGIVIGIA LIVLPHVGIK VTSTTEFCVS
60 70 80 90 100
CHSMQPVYEE YKQSVHFQNA SGVRAECHDC HIPPDIPGMV KRKLEASNDI
110 120 130 140 150
YQTFIAHSID TPEKFEAKRA ELAEREWARM KENNSATCRS CHNYDAMDHA
160 170 180 190 200
KQHPEAARQM KVAAKDNQSC IDCHKGIAHQ LPDMSSGFRK QFDELRASAN
210 220 230 240 250
DSGDTLYSID IKPIYAAKGD KEASGSLLPA SEVKVLKRDG DWLQIEITGW
260 270 280 290 300
TESAGRQRVL TQFPGKRIFV ASIRGDVQQQ VKTLEKTTVA DTNTEWSKLQ
310 320 330 340 350
ATAWMKKGDM VNDIKPIWAY ADSLYNGTCN QCHGAPEIAH FDANGWIGTL
360 370 380 390
NGMIGFTSLD KREERTLLKY LQMNASDTAG KAHGDKKEEK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 194 – 195 | EL → DV in CAA52094 (PubMed:8022286).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73888 Genomic DNA Translation: CAA52094.1 U00096 Genomic DNA Translation: AAC74081.1 AP009048 Genomic DNA Translation: BAA36138.1 |
PIRi | B64841, S34221 |
RefSeqi | NP_415516.1, NC_000913.3 WP_001230242.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC74081; AAC74081; b0996 BAA36138; BAA36138; BAA36138 |
GeneIDi | 946252 |
KEGGi | ecj:JW0981 eco:b0996 |
PATRICi | fig|1411691.4.peg.1275 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X73888 Genomic DNA Translation: CAA52094.1 U00096 Genomic DNA Translation: AAC74081.1 AP009048 Genomic DNA Translation: BAA36138.1 |
PIRi | B64841, S34221 |
RefSeqi | NP_415516.1, NC_000913.3 WP_001230242.1, NZ_SSZK01000002.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4261495, 10 interactors |
ComplexPortali | CPX-319, Trimethylamine-N-oxide reductase TorAC complex |
DIPi | DIP-11014N |
IntActi | P33226, 2 interactors |
STRINGi | 511145.b0996 |
Protein family/group databases
TCDBi | 5.A.3.4.1, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Proteomic databases
PaxDbi | P33226 |
PRIDEi | P33226 |
Genome annotation databases
EnsemblBacteriai | AAC74081; AAC74081; b0996 BAA36138; BAA36138; BAA36138 |
GeneIDi | 946252 |
KEGGi | ecj:JW0981 eco:b0996 |
PATRICi | fig|1411691.4.peg.1275 |
Organism-specific databases
EchoBASEi | EB1762 |
Phylogenomic databases
eggNOGi | COG3005, Bacteria |
HOGENOMi | CLU_058814_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11815-MONOMER MetaCyc:EG11815-MONOMER |
Miscellaneous databases
PROi | PR:P33226 |
Family and domain databases
Gene3Di | 1.10.3820.10, 1 hit |
InterProi | View protein in InterPro IPR009154, Membr-bd_4haem_cyt_TorC IPR011031, Multihaem_cyt IPR036280, Multihaem_cyt_sf IPR005126, NapC/NirT_cyt_c_N IPR038266, NapC/NirT_cytc_sf |
Pfami | View protein in Pfam PF03264, Cytochrom_NNT, 1 hit |
PIRSFi | PIRSF000014, 4_hem_cytch_TorC, 1 hit |
SUPFAMi | SSF48695, SSF48695, 1 hit |
TIGRFAMsi | TIGR02162, torC, 1 hit |
PROSITEi | View protein in PROSITE PS51008, MULTIHEME_CYTC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TORC_ECOLI | |
Accessioni | P33226Primary (citable) accession number: P33226 Secondary accession number(s): P77446 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 175 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families