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Protein

Putative acyl-CoA dehydrogenase AidB

Gene

aidB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the adaptive DNA-repair response to alkylating agents. Could prevent alkylation damage by protecting DNA and destroying alkylating agents that have yet to reach their DNA target. Binds to double-stranded DNA with a preference for a DNA region that includes its own promoter. Shows weak isovaleryl-CoA dehydrogenase activity in vitro.3 Publications

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185FAD1 Publication1
Binding sitei191FAD1 Publication1
Binding sitei218FAD1 Publication1
Binding sitei429FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi182 – 191FAD1 Publication10
Nucleotide bindingi216 – 218FAD1 Publication3
Nucleotide bindingi423 – 433FAD1 PublicationAdd BLAST11

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • identical protein binding Source: IntAct
  • isovaleryl-CoA dehydrogenase activity Source: EcoCyc
  • sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Oxidoreductase
Biological processStress response
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:EG11811-MONOMER
MetaCyc:EG11811-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative acyl-CoA dehydrogenase AidB (EC:1.3.99.-)
Gene namesi
Name:aidB
Ordered Locus Names:b4187, JW5867
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11811 aidB

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi437R → Q: Does not affect DNA binding affinity. 1 Publication1
Mutagenesisi518R → Q: Reduces DNA binding affinity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002011921 – 541Putative acyl-CoA dehydrogenase AidBAdd BLAST541

Proteomic databases

PaxDbiP33224
PRIDEiP33224

Expressioni

Inductioni

Regulated by Ada in response to alkylating agents. Also induced by anaerobiosis or by acetate at pH 6.5, via RpoS. Represses its own synthesis during normal cell growth.2 Publications

Interactioni

Subunit structurei

Homotetramer. Dimer of dimers.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-542726,EBI-542726

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4262695, 240 interactors
DIPiDIP-9078N
IntActiP33224, 32 interactors
STRINGi316385.ECDH10B_4382

Structurei

Secondary structure

1541
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP33224
SMRiP33224
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP33224

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni445 – 541dsDNA-bindingAdd BLAST97

Domaini

The N-terminal region contains FAD-dependent dehydrogenase activity and the C-terminal region contains DNA-binding activity.1 Publication

Sequence similaritiesi

Belongs to the acyl-CoA dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105CSU Bacteria
COG1960 LUCA
HOGENOMiHOG000253935
InParanoidiP33224
KOiK09456
OMAiGKYWICK
PhylomeDBiP33224

Family and domain databases

CDDicd01154 AidB, 1 hit
InterProiView protein in InterPro
IPR006089 Acyl-CoA_DH_CS
IPR006091 Acyl-CoA_Oxase/DH_cen-dom
IPR036250 AcylCo_DH-like_C
IPR009075 AcylCo_DH/oxidase_C
IPR009100 AcylCoA_DH/oxidase_NM_dom
IPR034184 AidB
PfamiView protein in Pfam
PF00441 Acyl-CoA_dh_1, 1 hit
PF02770 Acyl-CoA_dh_M, 1 hit
SUPFAMiSSF47203 SSF47203, 1 hit
SSF56645 SSF56645, 1 hit
PROSITEiView protein in PROSITE
PS00072 ACYL_COA_DH_1, 1 hit
PS00073 ACYL_COA_DH_2, 1 hit

Sequencei

Sequence statusi: Complete.

P33224-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHWQTHTVFN QPIPLNNSNL YLSDGALCEA VTREGAGWDS DFLASIGQQL
60 70 80 90 100
GTAESLELGR LANVNPPELL RYDAQGRRLD DVRFHPAWHL LMQALCTNRV
110 120 130 140 150
HNLAWEEDAR SGAFVARAAR FMLHAQVEAG SLCPITMTFA ATPLLLQMLP
160 170 180 190 200
APFQDWTTPL LSDRYDSHLL PGGQKRGLLI GMGMTEKQGG SDVMSNTTRA
210 220 230 240 250
ERLEDGSYRL VGHKWFFSVP QSDAHLVLAQ TAGGLSCFFV PRFLPDGQRN
260 270 280 290 300
AIRLERLKDK LGNRSNASCE VEFQDAIGWL LGLEGEGIRL ILKMGGMTRF
310 320 330 340 350
DCALGSHAMM RRAFSLAIYH AHQRHVFGNP LIQQPLMRHV LSRMALQLEG
360 370 380 390 400
QTALLFRLAR AWDRRADAKE ALWARLFTPA AKFVICKRGM PFVAEAMEVL
410 420 430 440 450
GGIGYCEESE LPRLYREMPV NSIWEGSGNI MCLDVLRVLN KQAGVYDLLS
460 470 480 490 500
EAFVEVKGQD RYFDRAVRRL QQQLRKPAEE LGREITHQLF LLGCGAQMLK
510 520 530 540
YASPPMAQAW CQVMLDTRGG VRLSEQIQND LLLRATGGVC V
Length:541
Mass (Da):60,590
Last modified:December 1, 2000 - v3
Checksum:iD609E364E3A99208
GO

Sequence cautioni

The sequence AAA97083 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAC18889 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20915 Genomic DNA Translation: AAC18889.1 Different initiation.
L20915 Genomic DNA Translation: AAC18890.1
U14003 Genomic DNA Translation: AAA97083.1 Different initiation.
U00096 Genomic DNA Translation: AAC77144.2
AP009048 Genomic DNA Translation: BAE78188.1
PIRiI41124
RefSeqiNP_418608.6, NC_000913.3
WP_001350567.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77144; AAC77144; b4187
BAE78188; BAE78188; BAE78188
GeneIDi948710
KEGGiecj:JW5867
eco:b4187
PATRICifig|1411691.4.peg.2514

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20915 Genomic DNA Translation: AAC18889.1 Different initiation.
L20915 Genomic DNA Translation: AAC18890.1
U14003 Genomic DNA Translation: AAA97083.1 Different initiation.
U00096 Genomic DNA Translation: AAC77144.2
AP009048 Genomic DNA Translation: BAE78188.1
PIRiI41124
RefSeqiNP_418608.6, NC_000913.3
WP_001350567.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DJLX-ray1.70A1-541[»]
3U33X-ray2.80A/B/C/D/E/F/G/H/I/J/K/L1-541[»]
ProteinModelPortaliP33224
SMRiP33224
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262695, 240 interactors
DIPiDIP-9078N
IntActiP33224, 32 interactors
STRINGi316385.ECDH10B_4382

Proteomic databases

PaxDbiP33224
PRIDEiP33224

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77144; AAC77144; b4187
BAE78188; BAE78188; BAE78188
GeneIDi948710
KEGGiecj:JW5867
eco:b4187
PATRICifig|1411691.4.peg.2514

Organism-specific databases

EchoBASEiEB1759
EcoGeneiEG11811 aidB

Phylogenomic databases

eggNOGiENOG4105CSU Bacteria
COG1960 LUCA
HOGENOMiHOG000253935
InParanoidiP33224
KOiK09456
OMAiGKYWICK
PhylomeDBiP33224

Enzyme and pathway databases

BioCyciEcoCyc:EG11811-MONOMER
MetaCyc:EG11811-MONOMER

Miscellaneous databases

EvolutionaryTraceiP33224
PROiPR:P33224

Family and domain databases

CDDicd01154 AidB, 1 hit
InterProiView protein in InterPro
IPR006089 Acyl-CoA_DH_CS
IPR006091 Acyl-CoA_Oxase/DH_cen-dom
IPR036250 AcylCo_DH-like_C
IPR009075 AcylCo_DH/oxidase_C
IPR009100 AcylCoA_DH/oxidase_NM_dom
IPR034184 AidB
PfamiView protein in Pfam
PF00441 Acyl-CoA_dh_1, 1 hit
PF02770 Acyl-CoA_dh_M, 1 hit
SUPFAMiSSF47203 SSF47203, 1 hit
SSF56645 SSF56645, 1 hit
PROSITEiView protein in PROSITE
PS00072 ACYL_COA_DH_1, 1 hit
PS00073 ACYL_COA_DH_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAIDB_ECOLI
AccessioniPrimary (citable) accession number: P33224
Secondary accession number(s): P33223, Q2M6B8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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