UniProtKB - P33224 (AIDB_ECOLI)
Protein
Putative acyl-CoA dehydrogenase AidB
Gene
aidB
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the adaptive DNA-repair response to alkylating agents. Could prevent alkylation damage by protecting DNA and destroying alkylating agents that have yet to reach their DNA target. Binds to double-stranded DNA with a preference for a DNA region that includes its own promoter. Shows weak isovaleryl-CoA dehydrogenase activity in vitro.3 Publications
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 185 | FAD1 Publication | 1 | |
Binding sitei | 191 | FAD1 Publication | 1 | |
Binding sitei | 218 | FAD1 Publication | 1 | |
Binding sitei | 429 | FAD1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 182 – 191 | FAD1 Publication | 10 | |
Nucleotide bindingi | 216 – 218 | FAD1 Publication | 3 | |
Nucleotide bindingi | 423 – 433 | FAD1 PublicationAdd BLAST | 11 |
GO - Molecular functioni
- DNA binding Source: EcoCyc
- identical protein binding Source: IntAct
- isovaleryl-CoA dehydrogenase activity Source: EcoCyc
- sequence-specific DNA binding Source: EcoCyc
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoCyc
- negative regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Oxidoreductase |
Biological process | Stress response |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11811-MONOMER MetaCyc:EG11811-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:aidB Ordered Locus Names:b4187, JW5867 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 437 | R → Q: Does not affect DNA binding affinity. 1 Publication | 1 | |
Mutagenesisi | 518 | R → Q: Reduces DNA binding affinity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201192 | 1 – 541 | Putative acyl-CoA dehydrogenase AidBAdd BLAST | 541 |
Proteomic databases
jPOSTi | P33224 |
PaxDbi | P33224 |
PRIDEi | P33224 |
Expressioni
Inductioni
Regulated by Ada in response to alkylating agents. Also induced by anaerobiosis or by acetate at pH 6.5, via RpoS. Represses its own synthesis during normal cell growth.2 Publications
Interactioni
Subunit structurei
Homotetramer. Dimer of dimers.
2 PublicationsBinary interactionsi
P33224
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-542726,EBI-542726 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262695, 240 interactors |
DIPi | DIP-9078N |
IntActi | P33224, 32 interactors |
STRINGi | 511145.b4187 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P33224 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P33224 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 445 – 541 | dsDNA-bindingAdd BLAST | 97 |
Domaini
The N-terminal region contains FAD-dependent dehydrogenase activity and the C-terminal region contains DNA-binding activity.1 Publication
Sequence similaritiesi
Belongs to the acyl-CoA dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | COG1960, Bacteria |
HOGENOMi | CLU_016513_0_0_6 |
InParanoidi | P33224 |
PhylomeDBi | P33224 |
Family and domain databases
CDDi | cd01154, AidB, 1 hit |
InterProi | View protein in InterPro IPR006089, Acyl-CoA_DH_CS IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR009100, AcylCoA_DH/oxidase_NM_dom IPR034184, AidB IPR041504, AidB_N |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF18158, AidB_N, 1 hit |
SUPFAMi | SSF47203, SSF47203, 1 hit SSF56645, SSF56645, 1 hit |
PROSITEi | View protein in PROSITE PS00072, ACYL_COA_DH_1, 1 hit PS00073, ACYL_COA_DH_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P33224-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHWQTHTVFN QPIPLNNSNL YLSDGALCEA VTREGAGWDS DFLASIGQQL
60 70 80 90 100
GTAESLELGR LANVNPPELL RYDAQGRRLD DVRFHPAWHL LMQALCTNRV
110 120 130 140 150
HNLAWEEDAR SGAFVARAAR FMLHAQVEAG SLCPITMTFA ATPLLLQMLP
160 170 180 190 200
APFQDWTTPL LSDRYDSHLL PGGQKRGLLI GMGMTEKQGG SDVMSNTTRA
210 220 230 240 250
ERLEDGSYRL VGHKWFFSVP QSDAHLVLAQ TAGGLSCFFV PRFLPDGQRN
260 270 280 290 300
AIRLERLKDK LGNRSNASCE VEFQDAIGWL LGLEGEGIRL ILKMGGMTRF
310 320 330 340 350
DCALGSHAMM RRAFSLAIYH AHQRHVFGNP LIQQPLMRHV LSRMALQLEG
360 370 380 390 400
QTALLFRLAR AWDRRADAKE ALWARLFTPA AKFVICKRGM PFVAEAMEVL
410 420 430 440 450
GGIGYCEESE LPRLYREMPV NSIWEGSGNI MCLDVLRVLN KQAGVYDLLS
460 470 480 490 500
EAFVEVKGQD RYFDRAVRRL QQQLRKPAEE LGREITHQLF LLGCGAQMLK
510 520 530 540
YASPPMAQAW CQVMLDTRGG VRLSEQIQND LLLRATGGVC V
Sequence cautioni
The sequence AAA97083 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence AAC18889 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L20915 Genomic DNA Translation: AAC18889.1 Different initiation. L20915 Genomic DNA Translation: AAC18890.1 U14003 Genomic DNA Translation: AAA97083.1 Different initiation. U00096 Genomic DNA Translation: AAC77144.2 AP009048 Genomic DNA Translation: BAE78188.1 |
PIRi | I41124 |
RefSeqi | NP_418608.6, NC_000913.3 WP_001350567.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77144; AAC77144; b4187 BAE78188; BAE78188; BAE78188 |
GeneIDi | 948710 |
KEGGi | ecj:JW5867 eco:b4187 |
PATRICi | fig|1411691.4.peg.2514 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L20915 Genomic DNA Translation: AAC18889.1 Different initiation. L20915 Genomic DNA Translation: AAC18890.1 U14003 Genomic DNA Translation: AAA97083.1 Different initiation. U00096 Genomic DNA Translation: AAC77144.2 AP009048 Genomic DNA Translation: BAE78188.1 |
PIRi | I41124 |
RefSeqi | NP_418608.6, NC_000913.3 WP_001350567.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3DJL | X-ray | 1.70 | A | 1-541 | [»] | |
3U33 | X-ray | 2.80 | A/B/C/D/E/F/G/H/I/J/K/L | 1-541 | [»] | |
SMRi | P33224 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262695, 240 interactors |
DIPi | DIP-9078N |
IntActi | P33224, 32 interactors |
STRINGi | 511145.b4187 |
Proteomic databases
jPOSTi | P33224 |
PaxDbi | P33224 |
PRIDEi | P33224 |
Genome annotation databases
EnsemblBacteriai | AAC77144; AAC77144; b4187 BAE78188; BAE78188; BAE78188 |
GeneIDi | 948710 |
KEGGi | ecj:JW5867 eco:b4187 |
PATRICi | fig|1411691.4.peg.2514 |
Organism-specific databases
EchoBASEi | EB1759 |
Phylogenomic databases
eggNOGi | COG1960, Bacteria |
HOGENOMi | CLU_016513_0_0_6 |
InParanoidi | P33224 |
PhylomeDBi | P33224 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11811-MONOMER MetaCyc:EG11811-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P33224 |
PROi | PR:P33224 |
Family and domain databases
CDDi | cd01154, AidB, 1 hit |
InterProi | View protein in InterPro IPR006089, Acyl-CoA_DH_CS IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR036250, AcylCo_DH-like_C IPR009075, AcylCo_DH/oxidase_C IPR009100, AcylCoA_DH/oxidase_NM_dom IPR034184, AidB IPR041504, AidB_N |
Pfami | View protein in Pfam PF00441, Acyl-CoA_dh_1, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF18158, AidB_N, 1 hit |
SUPFAMi | SSF47203, SSF47203, 1 hit SSF56645, SSF56645, 1 hit |
PROSITEi | View protein in PROSITE PS00072, ACYL_COA_DH_1, 1 hit PS00073, ACYL_COA_DH_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AIDB_ECOLI | |
Accessioni | P33224Primary (citable) accession number: P33224 Secondary accession number(s): P33223, Q2M6B8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | December 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families