UniProtKB - P33222 (YJFC_ECOLI)
Protein
Putative acid--amine ligase YjfC
Gene
yjfC
Organism
Escherichia coli (strain K12)
Status
Functioni
May be a ligase forming an amide bond. Shows ATPase activity. Despite its similarity to the C-terminal synthetase domain of Gss, is not a glutathionylspermidine (Gsp) synthetase. Cannot synthesize Gsp, glutathione (GSH), or GSH intermediates, from GSH and spermidine, cysteine and glutamate, gamma-glutamylcysteine and spermidine, and gamma-glutamylcysteine and glycine. Does not bind to Gsp.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 101 | Transition state stabilizerBy similarity | 1 | |
Metal bindingi | 103 | Magnesium 1By similarity | 1 | |
Metal bindingi | 116 | Magnesium 1By similarity | 1 | |
Metal bindingi | 116 | Magnesium 2By similarity | 1 | |
Metal bindingi | 118 | Magnesium 2By similarity | 1 | |
Binding sitei | 265 | ATPBy similarity | 1 | |
Binding sitei | 302 | ATPBy similarity | 1 | |
Binding sitei | 309 | ATP; via amide nitrogenBy similarity | 1 | |
Binding sitei | 337 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 101 – 103 | ATPBy similarity | 3 | |
Nucleotide bindingi | 372 – 374 | ATPBy similarity | 3 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ligase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11812-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:yjfC Ordered Locus Names:b4186, JW4144 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Deletion of this gene does not affect the ability to synthesize glutathionylspermidine. There is no visible differences between wild-type and mutant strain growth in LB media.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169746 | 1 – 387 | Putative acid--amine ligase YjfCAdd BLAST | 387 |
Proteomic databases
PaxDbi | P33222 |
PRIDEi | P33222 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263212, 5 interactors |
IntActi | P33222, 1 interactor |
STRINGi | 511145.b4186 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glutathionylspermidine synthase preATP-grasp family.Curated
Phylogenomic databases
eggNOGi | COG0754, Bacteria |
HOGENOMi | CLU_059175_0_0_6 |
InParanoidi | P33222 |
PhylomeDBi | P33222 |
Family and domain databases
InterProi | View protein in InterPro IPR005494, GSPS_pre-ATP-grasp-like_dom IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF03738, GSP_synth, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
i Sequence
Sequence statusi: Complete.
P33222-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRHNVPVRR DLDQIAADNG FDFHIIDNEI YWDESRAYRF TLRQIEEQIE
60 70 80 90 100
KPTAELHQMC LEVVDRAVKD EEILTQLAIP PLYWDVIAES WRARDPSLYG
110 120 130 140 150
RMDFAWCGNA PVKLLEYNAD TPTSLYESAY FQWLWLEDAR RSGIIPRDAD
160 170 180 190 200
QYNAIQERLI SRFSELYSRE PFYFCCCQDT DEDRSTVLYL QDCAQQAGQE
210 220 230 240 250
SRFIYIEDLG LGVGGVLTDL DDNVIQRAFK LYPLEWMMRD DNGPLLRKRR
260 270 280 290 300
EQWVEPLWKS ILSNKGLMPL LWRFFPGHPN LLASWFDGEK PQIAAGESYV
310 320 330 340 350
RKPIYSREGG NVTIFDGKNN VVDHADGDYA DEPMIYQAFQ PLPRFGDSYT
360 370 380
LIGSWIVDDE ACGMGIREDN TLITKDTSRF VPHYIAG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97082.1 U00096 Genomic DNA Translation: AAC77143.1 AP009048 Genomic DNA Translation: BAE78187.1 L20915 Genomic DNA Translation: AAC18888.1 |
PIRi | S56411 |
RefSeqi | NP_418607.1, NC_000913.3 WP_000943959.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77143; AAC77143; b4186 BAE78187; BAE78187; BAE78187 |
GeneIDi | 948699 |
KEGGi | ecj:JW4144 eco:b4186 |
PATRICi | fig|1411691.4.peg.2515 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97082.1 U00096 Genomic DNA Translation: AAC77143.1 AP009048 Genomic DNA Translation: BAE78187.1 L20915 Genomic DNA Translation: AAC18888.1 |
PIRi | S56411 |
RefSeqi | NP_418607.1, NC_000913.3 WP_000943959.1, NZ_LN832404.1 |
3D structure databases
SMRi | P33222 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263212, 5 interactors |
IntActi | P33222, 1 interactor |
STRINGi | 511145.b4186 |
Proteomic databases
PaxDbi | P33222 |
PRIDEi | P33222 |
Genome annotation databases
EnsemblBacteriai | AAC77143; AAC77143; b4186 BAE78187; BAE78187; BAE78187 |
GeneIDi | 948699 |
KEGGi | ecj:JW4144 eco:b4186 |
PATRICi | fig|1411691.4.peg.2515 |
Organism-specific databases
EchoBASEi | EB1760 |
Phylogenomic databases
eggNOGi | COG0754, Bacteria |
HOGENOMi | CLU_059175_0_0_6 |
InParanoidi | P33222 |
PhylomeDBi | P33222 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11812-MONOMER |
Miscellaneous databases
PROi | PR:P33222 |
Family and domain databases
InterProi | View protein in InterPro IPR005494, GSPS_pre-ATP-grasp-like_dom IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF03738, GSP_synth, 1 hit |
SUPFAMi | SSF52440, SSF52440, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YJFC_ECOLI | |
Accessioni | P33222Primary (citable) accession number: P33222 Secondary accession number(s): Q2M6B9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1995 | |
Last modified: | October 7, 2020 | |
This is version 124 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families