UniProtKB - P33202 (UFD4_YEAST)
Protein
Ubiquitin fusion degradation protein 4
Gene
UFD4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.By similarity
Miscellaneous
A cysteine residue is required for ubiquitin-thioester formation.
Present with 7380 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.26
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 1450 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- proteasome regulatory particle binding Source: SGD
- ubiquitin protein ligase activity Source: SGD
GO - Biological processi
- free ubiquitin chain polymerization Source: SGD
- negative regulation of telomerase activity Source: SGD
- protein K29-linked ubiquitination Source: SGD
- ubiquitin-dependent protein catabolic process Source: SGD
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
BioCyci | YEAST:G3O-31819-MONOMER |
Reactomei | R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:UFD4 Ordered Locus Names:YKL010C ORF Names:YKL162 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YKL010C |
SGDi | S000001493, UFD4 |
Subcellular locationi
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194998 | 1 – 1483 | Ubiquitin fusion degradation protein 4Add BLAST | 1483 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 87 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 349 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P33202 |
PaxDbi | P33202 |
PRIDEi | P33202 |
PTM databases
iPTMneti | P33202 |
Interactioni
Binary interactionsi
Hide detailsP33202
With | #Exp. | IntAct |
---|---|---|
MGT1 [P26188] | 2 | EBI-20010,EBI-10873 |
RPT6 [Q01939] | 3 | EBI-20010,EBI-13914 |
UFD2 [P54860] | 2 | EBI-20010,EBI-20003 |
Protein-protein interaction databases
BioGRIDi | 34122, 166 interactors |
DIPi | DIP-4873N |
IntActi | P33202, 30 interactors |
MINTi | P33202 |
STRINGi | 4932.YKL010C |
Miscellaneous databases
RNActi | P33202, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1376 – 1483 | HECTPROSITE-ProRule annotationAdd BLAST | 108 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1007 – 1081 | K-boxAdd BLAST | 75 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 898 – 901 | Poly-Glu | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0168, Eukaryota KOG0170, Eukaryota |
GeneTreei | ENSGT00940000156517 |
HOGENOMi | CLU_000366_1_1_1 |
InParanoidi | P33202 |
OMAi | HGFNMDS |
Family and domain databases
CDDi | cd00078, HECTc, 1 hit |
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase |
Pfami | View protein in Pfam PF00632, HECT, 1 hit |
SMARTi | View protein in SMART SM00119, HECTc, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50237, HECT, 1 hit |
i Sequence
Sequence statusi: Complete.
P33202-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSENNSHNLD EHESHSENSD YMMDTQVEDD YDEDGHVQGE YSYYPDEDED
60 70 80 90 100
EHMLSSVGSF EADDGEDDDN DYHHEDDSGL LYGYHRTQNG SDEDRNEEED
110 120 130 140 150
GLERSHDNNE FGSNPLHLPD ILETFAQRLE QRRQTSEGLG QHPVGRTLPE
160 170 180 190 200
ILSMIGGRME RSAESSARNE RISKLIENTG NASEDPYIAM ESLKELSENI
210 220 230 240 250
LMMNQMVVDR IIPMETLIGN IAAILSDKIL REELELQMQA CRCMYNLFEV
260 270 280 290 300
CPESISIAVD EHVIPILQGK LVEISYIDLA EQVLETVEYI SRVHGRDILK
310 320 330 340 350
TGQLSIYVQF FDFLTIHAQR KAIAIVSNAC SSIRTDDFKT IVEVLPTLKP
360 370 380 390 400
IFSNATDQPI LTRLVNAMYG ICGALHGVDK FETLFSLDLI ERIVQLVSIQ
410 420 430 440 450
DTPLENKLKC LDILTVLAMS SDVLSRELRE KTDIVDMATR SFQHYSKSPN
460 470 480 490 500
AGLHETLIYV PNSLLISISR FIVVLFPPED ERILSADKYT GNSDRGVISN
510 520 530 540 550
QEKFDSLVQC LIPILVEIYT NAADFDVRRY VLIALLRVVS CINNSTAKAI
560 570 580 590 600
NDQLIKLIGS ILAQKETASN ANGTYSSEAG TLLVGGLSLL DLICKKFSEL
610 620 630 640 650
FFPSIKREGI FDLVKDLSVD FNNIDLKEDG NENISLSDEE GDLHSSIEEC
660 670 680 690 700
DEGDEEYDYE FTDMEIPDSV KPKKISIHIF RTLSLAYIKN KGVNLVNRVL
710 720 730 740 750
SQMNVEQEAI TEELHQIEGV VSILENPSTP DKTEEDWKGI WSVLKKCIFH
760 770 780 790 800
EDFDVSGFEF TSTGLASSIT KRITSSTVSH FILAKSFLEV FEDCIDRFLE
810 820 830 840 850
ILQSALTRLE NFSIVDCGLH DGGGVSSLAK EIKIKLVYDG DASKDNIGTD
860 870 880 890 900
LSSTIVSVHC IASFTSLNEF LRHRMVRMRF LNSLIPNLTS SSTEADREEE
910 920 930 940 950
ENCLDHMRKK NFDFFYDNEK VDMESTVFGV IFNTFVRRNR DLKTLWDDTH
960 970 980 990 1000
TIKFCKSLEG NNRESEAAEE ANEGKKLRDF YKKREFAQVD TGSSADILTL
1010 1020 1030 1040 1050
LDFLHSCGVK SDSFINSKLS AKLARQLDEP LVVASGALPD WSLFLTRRFP
1060 1070 1080 1090 1100
FLFPFDTRML FLQCTSFGYG RLIQLWKNKS KGSKDLRNDE ALQQLGRITR
1110 1120 1130 1140 1150
RKLRISRKTI FATGLKILSK YGSSPDVLEI EYQEEAGTGL GPTLEFYSVV
1160 1170 1180 1190 1200
SKYFARKSLN MWRCNSYSYR SEMDVDTTDD YITTLLFPEP LNPFSNNEKV
1210 1220 1230 1240 1250
IELFGYLGTF VARSLLDNRI LDFRFSKVFF ELLHRMSTPN VTTVPSDVET
1260 1270 1280 1290 1300
CLLMIELVDP LLAKSLKYIV ANKDDNMTLE SLSLTFTVPG NDDIELIPGG
1310 1320 1330 1340 1350
CNKSLNSSNV EEYIHGVIDQ ILGKGIEKQL KAFIEGFSKV FSYERMLILF
1360 1370 1380 1390 1400
PDELVDIFGR VEEDWSMATL YTNLNAEHGY TMDSSIIHDF ISIISAFGKH
1410 1420 1430 1440 1450
ERRLFLQFLT GSPKLPIGGF KSLNPKFTVV LKHAEDGLTA DEYLPSVMTC
1460 1470 1480
ANYLKLPKYT SKDIMRSRLC QAIEEGAGAF LLS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S53418 Genomic DNA Translation: AAB24903.1 Z28010 Genomic DNA Translation: CAA81845.1 BK006944 Genomic DNA Translation: DAA09146.1 |
PIRi | S30015 |
RefSeqi | NP_012915.3, NM_001179576.3 |
Genome annotation databases
EnsemblFungii | YKL010C_mRNA; YKL010C; YKL010C |
GeneIDi | 853859 |
KEGGi | sce:YKL010C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S53418 Genomic DNA Translation: AAB24903.1 Z28010 Genomic DNA Translation: CAA81845.1 BK006944 Genomic DNA Translation: DAA09146.1 |
PIRi | S30015 |
RefSeqi | NP_012915.3, NM_001179576.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 34122, 166 interactors |
DIPi | DIP-4873N |
IntActi | P33202, 30 interactors |
MINTi | P33202 |
STRINGi | 4932.YKL010C |
PTM databases
iPTMneti | P33202 |
Proteomic databases
MaxQBi | P33202 |
PaxDbi | P33202 |
PRIDEi | P33202 |
Genome annotation databases
EnsemblFungii | YKL010C_mRNA; YKL010C; YKL010C |
GeneIDi | 853859 |
KEGGi | sce:YKL010C |
Organism-specific databases
EuPathDBi | FungiDB:YKL010C |
SGDi | S000001493, UFD4 |
Phylogenomic databases
eggNOGi | KOG0168, Eukaryota KOG0170, Eukaryota |
GeneTreei | ENSGT00940000156517 |
HOGENOMi | CLU_000366_1_1_1 |
InParanoidi | P33202 |
OMAi | HGFNMDS |
Enzyme and pathway databases
BioCyci | YEAST:G3O-31819-MONOMER |
Reactomei | R-SCE-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
PROi | PR:P33202 |
RNActi | P33202, protein |
Family and domain databases
CDDi | cd00078, HECTc, 1 hit |
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR000569, HECT_dom IPR035983, Hect_E3_ubiquitin_ligase |
Pfami | View protein in Pfam PF00632, HECT, 1 hit |
SMARTi | View protein in SMART SM00119, HECTc, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF56204, SSF56204, 1 hit |
PROSITEi | View protein in PROSITE PS50237, HECT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UFD4_YEAST | |
Accessioni | P33202Primary (citable) accession number: P33202 Secondary accession number(s): D6VXS6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD