Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kinesin heavy chain isoform 5A

Gene

Kif5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL) (PubMed:12682084). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons (PubMed:21976701). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 93ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, plus-end-directed Source: GO_Central
  • kinesin binding Source: UniProtKB
  • microtubule binding Source: MGI
  • microtubule motor activity Source: GO_Central
  • protein-containing complex binding Source: MGI
  • scaffold protein binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionMotor protein
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2132295 MHC class II antigen presentation
R-MMU-5625970 RHO GTPases activate KTN1
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-983189 Kinesins

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin heavy chain isoform 5A
Alternative name(s):
Kinesin heavy chain neuron-specific 1
Neuronal kinesin heavy chain
Short name:
NKHC
Gene namesi
Name:Kif5a
Synonyms:Kiaa4086, Kif5, Nkhc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:109564 Kif5a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Death shortly after birth. Neuron-specific deletion within the first 3 weeks after birth is lethal in 75% of animals. Surviving animals show accumulation of neurofilament proteins in neuronal soma, age-dependent sensory neuron degeneration, loss of large caliber axons, and hind limb paralysis with a stronger effect on sensory neurons compared with motor neurons.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi93T → N: Loss of ability to induce formation of neurite-like membrane protrusions in non-neuronal cells. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001253542 – 1027Kinesin heavy chain isoform 5AAdd BLAST1026

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei397PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP33175
PaxDbiP33175
PeptideAtlasiP33175
PRIDEiP33175

PTM databases

iPTMnetiP33175
PhosphoSitePlusiP33175

Expressioni

Gene expression databases

BgeeiENSMUSG00000074657 Expressed in 200 organ(s), highest expression level in cerebral cortex
CleanExiMM_KIF5A
ExpressionAtlasiP33175 baseline and differential
GenevisibleiP33175 MM

Interactioni

Subunit structurei

Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 (PubMed:11986669). Interacts with FMR1 (via C-terminus); this interaction is increased in a mGluR-dependent manner (PubMed:18539120). Interacts with BORCS5 (By similarity). Interacts with ZFYVE27 (PubMed:21976701, PubMed:24251978). Interacts with VAPA, VAPB, SURF4, RAB11A (GDP-bound form), RAB11B (GDP-bound form) and RTN3 in a ZFYVE27-dependent manner (PubMed:21976701). Interacts with BICD2 (By similarity).By similarity4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200945, 13 interactors
CORUMiP33175
IntActiP33175, 11 interactors
MINTiP33175
STRINGi10090.ENSMUSP00000096775

Structurei

3D structure databases

ProteinModelPortaliP33175
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 327Kinesin motorPROSITE-ProRule annotationAdd BLAST319

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni174 – 315Microtubule-bindingAdd BLAST142
Regioni271 – 361Necessary for interaction with ZFYVE271 PublicationAdd BLAST91
Regioni353 – 1027Interaction with BICD2By similarityAdd BLAST675
Regioni907 – 1027GlobularAdd BLAST121

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili331 – 906Add BLAST576

Domaini

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0240 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00930000150825
HOGENOMiHOG000216718
HOVERGENiHBG006210
InParanoidiP33175
KOiK10396
OMAiEEDFTMA
OrthoDBiEOG091G02A2
PhylomeDBiP33175
TreeFamiTF105225

Family and domain databases

Gene3Di3.40.850.10, 1 hit
InterProiView protein in InterPro
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P33175-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAETNNECSI KVLCRFRPLN QAEILRGDKF IPIFQGDDSV IIGGKPYVFD
60 70 80 90 100
RVFPPNTTQE QVYHACAMQI VKDVLAGYNG TIFAYGQTSS GKTHTMEGKL
110 120 130 140 150
HDPQLMGIIP RIARDIFNHI YSMDENLEFH IKVSYFEIYL DKIRDLLDVT
160 170 180 190 200
KTNLSVHEDK NRVPFVKGCT ERFVSSPEEI LDVIDEGKSN RHVAVTNMNE
210 220 230 240 250
HSSRSHSIFL INIKQENVET EQKLSGKLYL VDLAGSEKVS KTGAEGAVLD
260 270 280 290 300
EAKNINKSLS ALGNVISALA EGTKSYVPYR DSKMTRILQD SLGGNCRTTM
310 320 330 340 350
FICCSPSSYN DAETKSTLMF GQRAKTIKNT ASVNLELTAE QWKKKYEKEK
360 370 380 390 400
EKTKAQKETI AKLEAELSRW RNGENVPETE RLAGEDSALG AELCEETPVN
410 420 430 440 450
DNSSIVVRIA PEERQKYEEE IRRLYKQLDD KDDEINQQSQ LIEKLKQQML
460 470 480 490 500
DQEELLVSTR GDNEKVQREL SHLQSENDAA KDEVKEVLQA LEELAVNYDQ
510 520 530 540 550
KSQEVEEKSQ QNQLLVDELS QKVATMLSLE SELQRLQEVS GHQRKRIAEV
560 570 580 590 600
LNGLMRDLSE FSVIVGNGEI KLPVEISGAI EEEFTVARLY ISKIKSEVKS
610 620 630 640 650
VVKRCRQLEN LQVECHRKME VTGRELSSCQ LLISQHEAKI RSLTEYMQTV
660 670 680 690 700
ELKKRHLEES YDSLSDELAR LQAHETVHEV ALKDKEPDTQ DAEEVKKALE
710 720 730 740 750
LQMENHREAH HRQLARLRDE INEKQKTIDE LKDLNQKLQL ELEKLQADYE
760 770 780 790 800
RLKNEENEKS AKLQELTFLY ERHEQSKQDL KGLEETVARE LQTLHNLRKL
810 820 830 840 850
FVQDVTTRVK KSAEMEPEDS GGIHSQKQKI SFLENNLEQL TKVHKQLVRD
860 870 880 890 900
NADLRCELPK LEKRLRATAE RVKALEGALK EAKEGAMKDK RRYQQEVDRI
910 920 930 940 950
KEAVRYKSSG KRGHSAQIAK PVRPGHYPAS SPTNPYGTRS PECISYTNNL
960 970 980 990 1000
FQNYQNLHLQ AAPSSTSDMY FASSGATSVA PLASYQKANM DNGNATDIND
1010 1020
NRSDLPCGYE AEDQAKLFPL HQETAAS
Length:1,027
Mass (Da):117,019
Last modified:June 27, 2006 - v3
Checksum:i419AD8C2D747DA0A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P859A0A1W2P859_MOUSE
Kinesin heavy chain isoform 5A
Kif5a
42Annotation score:

Sequence cautioni

The sequence BAD90503 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146L → P (PubMed:1447303).Curated1
Sequence conflicti282S → T in AAC79803 (PubMed:9782088).Curated1
Sequence conflicti362 – 363KL → NV in AAC79803 (PubMed:9782088).Curated2
Sequence conflicti727T → I in BAD90503 (Ref. 3) Curated1
Sequence conflicti976A → R in AAC79803 (PubMed:9782088).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067179 mRNA Translation: AAC79803.1
AK147352 mRNA Translation: BAE27862.1
AK147660 mRNA Translation: BAE28054.1
AK147707 mRNA Translation: BAE28087.1
AK220479 mRNA Translation: BAD90503.1 Different initiation.
BC058396 mRNA Translation: AAH58396.1
CCDSiCCDS24233.1
PIRiC44259
RefSeqiNP_001034089.1, NM_001039000.4
NP_032473.2, NM_008447.4
UniGeneiMm.30355

Genome annotation databases

EnsembliENSMUST00000099172; ENSMUSP00000096775; ENSMUSG00000074657
ENSMUST00000217895; ENSMUSP00000151402; ENSMUSG00000074657
GeneIDi16572
KEGGimmu:16572
UCSCiuc007his.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067179 mRNA Translation: AAC79803.1
AK147352 mRNA Translation: BAE27862.1
AK147660 mRNA Translation: BAE28054.1
AK147707 mRNA Translation: BAE28087.1
AK220479 mRNA Translation: BAD90503.1 Different initiation.
BC058396 mRNA Translation: AAH58396.1
CCDSiCCDS24233.1
PIRiC44259
RefSeqiNP_001034089.1, NM_001039000.4
NP_032473.2, NM_008447.4
UniGeneiMm.30355

3D structure databases

ProteinModelPortaliP33175
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200945, 13 interactors
CORUMiP33175
IntActiP33175, 11 interactors
MINTiP33175
STRINGi10090.ENSMUSP00000096775

PTM databases

iPTMnetiP33175
PhosphoSitePlusiP33175

Proteomic databases

MaxQBiP33175
PaxDbiP33175
PeptideAtlasiP33175
PRIDEiP33175

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099172; ENSMUSP00000096775; ENSMUSG00000074657
ENSMUST00000217895; ENSMUSP00000151402; ENSMUSG00000074657
GeneIDi16572
KEGGimmu:16572
UCSCiuc007his.2 mouse

Organism-specific databases

CTDi3798
MGIiMGI:109564 Kif5a
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0240 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00930000150825
HOGENOMiHOG000216718
HOVERGENiHBG006210
InParanoidiP33175
KOiK10396
OMAiEEDFTMA
OrthoDBiEOG091G02A2
PhylomeDBiP33175
TreeFamiTF105225

Enzyme and pathway databases

ReactomeiR-MMU-2132295 MHC class II antigen presentation
R-MMU-5625970 RHO GTPases activate KTN1
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-983189 Kinesins

Miscellaneous databases

ChiTaRSiKif5a mouse
PROiPR:P33175
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074657 Expressed in 200 organ(s), highest expression level in cerebral cortex
CleanExiMM_KIF5A
ExpressionAtlasiP33175 baseline and differential
GenevisibleiP33175 MM

Family and domain databases

Gene3Di3.40.850.10, 1 hit
InterProiView protein in InterPro
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKIF5A_MOUSE
AccessioniPrimary (citable) accession number: P33175
Secondary accession number(s): Q5DTP1, Q6PDY7, Q9Z2F9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 27, 2006
Last modified: November 7, 2018
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again