UniProtKB - P33174 (KIF4_MOUSE)
Protein
Chromosome-associated kinesin KIF4
Gene
Kif4
Organism
Mus musculus (Mouse)
Status
Functioni
Iron-sulfur (Fe-S) cluster binding motor protein that has a role in chromosome segregation during mitosis (By similarity). Required for mitotic chromosomal positioning and bipolar spindle stabilization.By similarity1 Publication
Cofactori
[2Fe-2S] clusterBy similarity, [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster (By similarity). In the presence of oxygen, the [4Fe-4S] cluster may be converted to [2Fe-2S] (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 88 – 95 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- iron-sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- microtubule binding Source: InterPro
- microtubule motor activity Source: InterPro
GO - Biological processi
- microtubule-based movement Source: InterPro
- mitotic cytokinesis Source: UniProtKB
- mitotic spindle midzone assembly Source: UniProtKB
- mitotic spindle organization Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Motor protein |
Ligand | ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2132295, MHC class II antigen presentation R-MMU-437239, Recycling pathway of L1 R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-MMU-983189, Kinesins |
Names & Taxonomyi
Protein namesi | Recommended name: Chromosome-associated kinesin KIF4Alternative name(s): Chromokinesin |
Gene namesi | Name:Kif4 Synonyms:Kif4a, Kns4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:108389, Kif4 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Nucleus
- Nucleus 1 Publication
Other locations
- Chromosome 1 Publication
Cytoskeleton
- microtubule Source: UniProtKB-KW
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: MGI
Other locations
- chromosome Source: UniProtKB-SubCell
- midbody Source: MGI
Keywords - Cellular componenti
Chromosome, Cytoplasm, Cytoskeleton, Microtubule, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000125438 | 1 – 1231 | Chromosome-associated kinesin KIF4Add BLAST | 1231 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 395 | PhosphoserineCombined sources | 1 | |
Modified residuei | 800 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 802 | PhosphoserineCombined sources | 1 | |
Modified residuei | 811 | PhosphoserineCombined sources | 1 | |
Modified residuei | 816 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1224 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1230 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P33174 |
jPOSTi | P33174 |
MaxQBi | P33174 |
PaxDbi | P33174 |
PeptideAtlasi | P33174 |
PRIDEi | P33174 |
PTM databases
iPTMneti | P33174 |
PhosphoSitePlusi | P33174 |
SwissPalmi | P33174 |
Expressioni
Tissue specificityi
Expressed in pyramidal cells in juvenile hippocampus, granular cells in juvenile cerebellar cortex and in adult spleen.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000034311, Expressed in primary oocyte and 220 other tissues |
Genevisiblei | P33174, MM |
Interactioni
GO - Molecular functioni
- microtubule binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 200944, 38 interactors |
IntActi | P33174, 29 interactors |
STRINGi | 10090.ENSMUSP00000048383 |
Miscellaneous databases
RNActi | P33174, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P33174 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 337 | Kinesin motorPROSITE-ProRule annotationAdd BLAST | 329 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1001 – 1231 | GlobularAdd BLAST | 231 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 351 – 1000 | Sequence analysisAdd BLAST | 650 |
Sequence similaritiesi
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Chromokinesin subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0244, Eukaryota |
GeneTreei | ENSGT00940000158195 |
HOGENOMi | CLU_001485_4_1_1 |
InParanoidi | P33174 |
OMAi | GCLELGN |
OrthoDBi | 369179at2759 |
TreeFami | TF105224 |
Family and domain databases
Gene3Di | 3.40.850.10, 1 hit |
InterProi | View protein in InterPro IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase IPR033467, Tesmin/TSO1-like_CXC |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM01114, CXC, 1 hit SM00129, KISc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P33174-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKEEVKGIPV RVALRCRPLV SKEIKEGCQT CLSFVPGEPQ VVVGNDKSFT
60 70 80 90 100
YDFVFDPSTE QEEVFNTAVA PLIKGVFKGY NATVLAYGQT GSGKTYSMGG
110 120 130 140 150
AYTAEQEHDS AIGVIPRVIQ LLFKEINKKS DFEFTLKVSY LEIYNEEILD
160 170 180 190 200
LLCSSREKAT QINIREDPKE GIKIVGLTEK TVLVASDTVS CLEQGNNSRT
210 220 230 240 250
VASTAMNSQS SRSHAIFTIS IEQRKKNDKN SSFRSKLHLV DLAGSERQKK
260 270 280 290 300
TKAEGDRLRE GININRGLLC LGNVISALGD DKKGNFVPYR DSKLTRLLQD
310 320 330 340 350
SLGGNSHTLM IACVSPADSN LEETLNTLRY ADRARKIKNK PIINIDPQAA
360 370 380 390 400
ELNHLKQQVQ QLQILLLQAH GGTLPGDINV EPSENLQSLM EKNQSLVEEN
410 420 430 440 450
EKLSRGLSEA AGQTAQMLER IILTEQANEK MNAKLEELRR HAACKVDLQK
460 470 480 490 500
LVETLEDQEL KENIEIICNL QQVIAQLSDE AAACMTATID TAGEADTQVQ
510 520 530 540 550
SSPDTSRSSD VFSTQHALRQ AQMSKELIEL NKALALKEAL AKKMTQNDNQ
560 570 580 590 600
LQPIQFQYQD NIKNLESEVL SLQREKEELV LELQTAKKDA NQAKLSERRR
610 620 630 640 650
KRLQELEGQI ADLKKKLQEQ SKLLKLKEST EHTVSKLNQE IRMMKNQRVQ
660 670 680 690 700
LMRQMKEDAE KFRQWKQQKD KEVIQLKERD RKRQYELLKL ERNFQKQSNV
710 720 730 740 750
LRRKTEEAAA ANKRLKDALQ KQKEVAEKRK ETQSRGMEST AARMKNWLGN
760 770 780 790 800
EIEVMVSTEE AKRHLNGLLE ERKILAQDVA QLKEKRESGE NPPLKLRRRT
810 820 830 840 850
FSYDEIHGQD SGAEDSIAKQ IESLETELEL RSAQIADLQQ KLLDAESEDR
860 870 880 890 900
PKQRWESIAT ILEAKCAIKY LVGELVSSKI LVSKLESSLN QSKASCIDVQ
910 920 930 940 950
KMLFEEQNHF AKIETELKEE LVKVEQQHQE KVLYLLSQLQ QSQMTEKQLE
960 970 980 990 1000
ESVSEKEQQL LSTLKCQEEE LRKMQEVCEQ NQQLLQENSA IKQKLTLLQV
1010 1020 1030 1040 1050
ASKQKPHLTR NIFQSPDSSF EYIPPKPKPC RIKEKCLEQS FAVEGLQYYS
1060 1070 1080 1090 1100
EPSVAEQDNE DSDDHADEEW IPTKLVKVSK KSIQGCSCKG WCGNKQCGCR
1110 1120 1130 1140 1150
KQKSDCNVSC SCDPTKCRNR HQNQDNSDAI ELNQDSENSF KLEDPTEVTS
1160 1170 1180 1190 1200
GLSFFHPICA TPSSKILKEM CDADQVQLKQ PVFVSSSDHP ELKSIASESQ
1210 1220 1230
ENKAIGKKKK RALASNTSFF SGCSPIQEES H
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 112 | I → S (PubMed:19468303).Curated | 1 | |
Sequence conflicti | 1044 – 1045 | EG → GE in BAA02167 (PubMed:7929562).Curated | 2 | |
Sequence conflicti | 1182 | V → M in BAA02167 (PubMed:7929562).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D12646 mRNA Translation: BAA02167.1 BX005480 Genomic DNA No translation available. BX276129 Genomic DNA No translation available. CH466564 Genomic DNA Translation: EDL14171.1 BC050946 mRNA Translation: AAH50946.1 |
CCDSi | CCDS30305.1 |
PIRi | A54803 |
RefSeqi | NP_032472.2, NM_008446.2 |
Genome annotation databases
Ensembli | ENSMUST00000048962; ENSMUSP00000048383; ENSMUSG00000034311 |
GeneIDi | 16571 |
KEGGi | mmu:16571 |
UCSCi | uc009twh.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D12646 mRNA Translation: BAA02167.1 BX005480 Genomic DNA No translation available. BX276129 Genomic DNA No translation available. CH466564 Genomic DNA Translation: EDL14171.1 BC050946 mRNA Translation: AAH50946.1 |
CCDSi | CCDS30305.1 |
PIRi | A54803 |
RefSeqi | NP_032472.2, NM_008446.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3ZFC | X-ray | 1.80 | A | 1-344 | [»] | |
3ZFD | X-ray | 1.71 | A | 1-344 | [»] | |
SMRi | P33174 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 200944, 38 interactors |
IntActi | P33174, 29 interactors |
STRINGi | 10090.ENSMUSP00000048383 |
PTM databases
iPTMneti | P33174 |
PhosphoSitePlusi | P33174 |
SwissPalmi | P33174 |
Proteomic databases
EPDi | P33174 |
jPOSTi | P33174 |
MaxQBi | P33174 |
PaxDbi | P33174 |
PeptideAtlasi | P33174 |
PRIDEi | P33174 |
Protocols and materials databases
Antibodypediai | 27394, 212 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000048962; ENSMUSP00000048383; ENSMUSG00000034311 |
GeneIDi | 16571 |
KEGGi | mmu:16571 |
UCSCi | uc009twh.2, mouse |
Organism-specific databases
CTDi | 16571 |
MGIi | MGI:108389, Kif4 |
Phylogenomic databases
eggNOGi | KOG0244, Eukaryota |
GeneTreei | ENSGT00940000158195 |
HOGENOMi | CLU_001485_4_1_1 |
InParanoidi | P33174 |
OMAi | GCLELGN |
OrthoDBi | 369179at2759 |
TreeFami | TF105224 |
Enzyme and pathway databases
Reactomei | R-MMU-2132295, MHC class II antigen presentation R-MMU-437239, Recycling pathway of L1 R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-MMU-983189, Kinesins |
Miscellaneous databases
BioGRID-ORCSi | 16571, 7 hits in 18 CRISPR screens |
ChiTaRSi | Kif4, mouse |
PROi | PR:P33174 |
RNActi | P33174, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034311, Expressed in primary oocyte and 220 other tissues |
Genevisiblei | P33174, MM |
Family and domain databases
Gene3Di | 3.40.850.10, 1 hit |
InterProi | View protein in InterPro IPR019821, Kinesin_motor_CS IPR001752, Kinesin_motor_dom IPR036961, Kinesin_motor_dom_sf IPR027417, P-loop_NTPase IPR033467, Tesmin/TSO1-like_CXC |
Pfami | View protein in Pfam PF00225, Kinesin, 1 hit |
PRINTSi | PR00380, KINESINHEAVY |
SMARTi | View protein in SMART SM01114, CXC, 1 hit SM00129, KISc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00411, KINESIN_MOTOR_1, 1 hit PS50067, KINESIN_MOTOR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KIF4_MOUSE | |
Accessioni | P33174Primary (citable) accession number: P33174 Secondary accession number(s): Q80YP3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 168 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families