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Protein

Elongation factor Tu

Gene

tuf

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 26GTPUniRule annotation8
Nucleotide bindingi82 – 86GTPUniRule annotation5
Nucleotide bindingi137 – 140GTPUniRule annotation4

GO - Molecular functioni

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU01130-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Alternative name(s):
P-40
Gene namesi
Name:tufUniRule annotation
Synonyms:tufA
Ordered Locus Names:BSU01130
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi385T → V: Not phosphorylated by PrkC in vitro. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000912911 – 396Elongation factor TuAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei385Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylated on Thr-385 in vitro by PrkC in the presence of poly-L-lysine or myelin basic protein, dephosphorylated by PrpC.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP33166
PRIDEiP33166

PTM databases

iPTMnetiP33166

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

Protein-protein interaction databases

DIPiDIP-52428N
IntActiP33166, 7 interactors
MINTiP33166
STRINGi224308.Bsubs1_010100000585

Structurei

3D structure databases

ProteinModelPortaliP33166
SMRiP33166
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 205tr-type GAdd BLAST196

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 26G1By similarity8
Regioni61 – 65G2By similarity5
Regioni82 – 85G3By similarity4
Regioni137 – 140G4By similarity4
Regioni175 – 177G5By similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CGV Bacteria
COG0050 LUCA
HOGENOMiHOG000229290
InParanoidiP33166
KOiK02358
OMAiYGHIDCP
PhylomeDBiP33166

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
HAMAPiMF_00118_B EF_Tu_B, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P33166-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKEKFDRSK SHANIGTIGH VDHGKTTLTA AITTVLHKKS GKGTAMAYDQ
60 70 80 90 100
IDGAPEERER GITISTAHVE YETETRHYAH VDCPGHADYV KNMITGAAQM
110 120 130 140 150
DGAILVVSAA DGPMPQTREH ILLSKNVGVP YIVVFLNKCD MVDDEELLEL
160 170 180 190 200
VEMEVRDLLS EYDFPGDDVP VVKGSALKAL EGDAEWEAKI FELMDAVDEY
210 220 230 240 250
IPTPERDTEK PFMMPVEDVF SITGRGTVAT GRVERGQVKV GDEVEIIGLQ
260 270 280 290 300
EENKKTTVTG VEMFRKLLDY AEAGDNIGAL LRGVSREEIQ RGQVLAKPGT
310 320 330 340 350
ITPHSKFKAE VYVLSKEEGG RHTPFFSNYR PQFYFRTTDV TGIIHLPEGV
360 370 380 390
EMVMPGDNTE MNVELISTIA IEEGTRFSIR EGGRTVGSGV VSTITE
Length:396
Mass (Da):43,593
Last modified:October 1, 1993 - v1
Checksum:iBC096F47AAE6F72A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D64127 Genomic DNA Translation: BAA11004.1
AL009126 Genomic DNA Translation: CAB11889.1
PIRiA60663
RefSeqiNP_387994.1, NC_000964.3
WP_003235058.1, NZ_JNCM01000029.1

Genome annotation databases

EnsemblBacteriaiCAB11889; CAB11889; BSU01130
GeneIDi935965
KEGGibsu:BSU01130
PATRICifig|224308.179.peg.116

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D64127 Genomic DNA Translation: BAA11004.1
AL009126 Genomic DNA Translation: CAB11889.1
PIRiA60663
RefSeqiNP_387994.1, NC_000964.3
WP_003235058.1, NZ_JNCM01000029.1

3D structure databases

ProteinModelPortaliP33166
SMRiP33166
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-52428N
IntActiP33166, 7 interactors
MINTiP33166
STRINGi224308.Bsubs1_010100000585

PTM databases

iPTMnetiP33166

Proteomic databases

PaxDbiP33166
PRIDEiP33166

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11889; CAB11889; BSU01130
GeneIDi935965
KEGGibsu:BSU01130
PATRICifig|224308.179.peg.116

Phylogenomic databases

eggNOGiENOG4105CGV Bacteria
COG0050 LUCA
HOGENOMiHOG000229290
InParanoidiP33166
KOiK02358
OMAiYGHIDCP
PhylomeDBiP33166

Enzyme and pathway databases

BioCyciBSUB:BSU01130-MONOMER

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
HAMAPiMF_00118_B EF_Tu_B, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEFTU_BACSU
AccessioniPrimary (citable) accession number: P33166
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 10, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
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Main funding by: National Institutes of Health

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