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Protein

Long-chain-fatty-acid--CoA ligase 1

Gene

ACSL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS07766-MONOMER
BRENDAi6.2.1.3 2681
ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-2046105 Linoleic acid (LA) metabolism
R-HSA-2046106 alpha-linolenic acid (ALA) metabolism
R-HSA-75876 Synthesis of very long-chain fatty acyl-CoAs
SIGNORiP33121

Chemistry databases

SwissLipidsiSLP:000000199
SLP:000001468 [P33121-1]

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 1 (EC:6.2.1.3)
Alternative name(s):
Acyl-CoA synthetase 1
Short name:
ACS1
Long-chain acyl-CoA synthetase 1
Short name:
LACS 1
Long-chain acyl-CoA synthetase 2
Short name:
LACS 2
Long-chain fatty acid-CoA ligase 2
Palmitoyl-CoA ligase 1
Palmitoyl-CoA ligase 2
Gene namesi
Name:ACSL1
Synonyms:FACL1, FACL2, LACS, LACS1, LACS2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000151726.13
HGNCiHGNC:3569 ACSL1
MIMi152425 gene
neXtProtiNX_P33121

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei25 – 45Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini46 – 698CytoplasmicSequence analysisAdd BLAST653

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

Pathology & Biotechi

Organism-specific databases

DisGeNETi2180
OpenTargetsiENSG00000151726
PharmGKBiPA27966

Chemistry databases

DrugBankiDB00131 Adenosine monophosphate
DB00171 Adenosine triphosphate

Polymorphism and mutation databases

BioMutaiACSL1
DMDMi417241

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931041 – 698Long-chain-fatty-acid--CoA ligase 1Add BLAST698

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei9Nitrated tyrosineBy similarity1
Modified residuei84PhosphotyrosineBy similarity1
Glycosylationi135O-linked (GlcNAc) serineBy similarity1
Modified residuei207N6-acetyllysineBy similarity1
Modified residuei356N6-acetyllysineBy similarity1
Modified residuei386N6-acetyllysineBy similarity1
Modified residuei620PhosphoserineCombined sources1
Modified residuei632N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Glycoprotein, Nitration, Phosphoprotein

Proteomic databases

EPDiP33121
MaxQBiP33121
PaxDbiP33121
PeptideAtlasiP33121
PRIDEiP33121
ProteomicsDBi54898
54899 [P33121-2]

PTM databases

iPTMnetiP33121
PhosphoSitePlusiP33121
SwissPalmiP33121

Expressioni

Tissue specificityi

Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.1 Publication

Developmental stagei

Expressed during the early stages of erythroid development while expression is very low in reticulocytes and young erythrocytes.

Gene expression databases

BgeeiENSG00000151726
CleanExiHS_ACSL1
ExpressionAtlasiP33121 baseline and differential
GenevisibleiP33121 HS

Organism-specific databases

HPAiHPA011316
HPA011964

Interactioni

Protein-protein interaction databases

BioGridi108476, 13 interactors
IntActiP33121, 4 interactors
STRINGi9606.ENSP00000281455

Structurei

3D structure databases

ProteinModelPortaliP33121
SMRiP33121
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1256 Eukaryota
COG1022 LUCA
GeneTreeiENSGT00690000101725
HOGENOMiHOG000159459
HOVERGENiHBG050452
InParanoidiP33121
KOiK01897
OMAiECVMLCH
OrthoDBiEOG091G03G9
PhylomeDBiP33121
TreeFamiTF313877

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P33121-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQAHELFRYF RMPELVDFRQ YVRTLPTNTL MGFGAFAALT TFWYATRPKP
60 70 80 90 100
LKPPCDLSMQ SVEVAGSGGA RRSALLDSDE PLVYFYDDVT TLYEGFQRGI
110 120 130 140 150
QVSNNGPCLG SRKPDQPYEW LSYKQVAELS ECIGSALIQK GFKTAPDQFI
160 170 180 190 200
GIFAQNRPEW VIIEQGCFAY SMVIVPLYDT LGNEAITYIV NKAELSLVFV
210 220 230 240 250
DKPEKAKLLL EGVENKLIPG LKIIVVMDAY GSELVERGQR CGVEVTSMKA
260 270 280 290 300
MEDLGRANRR KPKPPAPEDL AVICFTSGTT GNPKGAMVTH RNIVSDCSAF
310 320 330 340 350
VKATENTVNP CPDDTLISFL PLAHMFERVV ECVMLCHGAK IGFFQGDIRL
360 370 380 390 400
LMDDLKVLQP TVFPVVPRLL NRMFDRIFGQ ANTTLKRWLL DFASKRKEAE
410 420 430 440 450
LRSGIIRNNS LWDRLIFHKV QSSLGGRVRL MVTGAAPVSA TVLTFLRAAL
460 470 480 490 500
GCQFYEGYGQ TECTAGCCLT MPGDWTAGHV GAPMPCNLIK LVDVEEMNYM
510 520 530 540 550
AAEGEGEVCV KGPNVFQGYL KDPAKTAEAL DKDGWLHTGD IGKWLPNGTL
560 570 580 590 600
KIIDRKKHIF KLAQGEYIAP EKIENIYMRS EPVAQVFVHG ESLQAFLIAI
610 620 630 640 650
VVPDVETLCS WAQKRGFEGS FEELCRNKDV KKAILEDMVR LGKDSGLKPF
660 670 680 690
EQVKGITLHP ELFSIDNGLL TPTMKAKRPE LRNYFRSQID DLYSTIKV
Length:698
Mass (Da):77,943
Last modified:October 1, 1993 - v1
Checksum:iFD6669453589D362
GO
Isoform 2 (identifier: P33121-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     508-517: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:688
Mass (Da):76,871
Checksum:i815DF2AA8EE8A6B4
GO
Isoform 3 (identifier: P33121-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     306-331: NTVNPCPDDTLISFLPLAHMFERVVE → KALPLSASDTHISYLPLAHIYEQLLK

Note: No experimental confirmation available.
Show »
Length:698
Mass (Da):77,907
Checksum:i1EDBB04923DD129F
GO

Sequence cautioni

The sequence BAC04704 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21 – 23YVR → CV in AAB00959 (PubMed:8584017).Curated3
Sequence conflicti37 – 38AA → SRR in AAB00959 (PubMed:8584017).Curated2
Sequence conflicti44 – 47YATR → RPRH in AAB00959 (PubMed:8584017).Curated4
Sequence conflicti55 – 56CD → WH in AAB00959 (PubMed:8584017).Curated2
Sequence conflicti229A → S in AAB00959 (PubMed:8584017).Curated1
Sequence conflicti385L → V in AAB00959 (PubMed:8584017).Curated1
Sequence conflicti400 – 401EL → DV in AAB00959 (PubMed:8584017).Curated2
Sequence conflicti477A → T in AAB00959 (PubMed:8584017).Curated1
Sequence conflicti492 – 494VDV → GWQL in AAB00959 (PubMed:8584017).Curated3
Sequence conflicti502A → S in AAB00959 (PubMed:8584017).Curated1
Sequence conflicti695T → I in AAB00959 (PubMed:8584017).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054391306 – 331NTVNP…ERVVE → KALPLSASDTHISYLPLAHI YEQLLK in isoform 3. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_009604508 – 517Missing in isoform 2. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10040 mRNA Translation: BAA00931.1
L09229 mRNA Translation: AAB00959.1
AK096117 mRNA Translation: BAC04704.1 Different initiation.
AK296826 mRNA Translation: BAH12438.1
AC079257 Genomic DNA No translation available.
AC084871 Genomic DNA No translation available.
CH471056 Genomic DNA Translation: EAX04662.1
CH471056 Genomic DNA Translation: EAX04663.1
CH471056 Genomic DNA Translation: EAX04665.1
BC026290 mRNA Translation: AAH26290.1
BC050073 mRNA Translation: AAH50073.1
CCDSiCCDS3839.1 [P33121-1]
CCDS68826.1 [P33121-3]
PIRiJX0202
RefSeqiNP_001273637.1, NM_001286708.1 [P33121-1]
NP_001273639.1, NM_001286710.1 [P33121-3]
NP_001273640.1, NM_001286711.1
NP_001273641.1, NM_001286712.1
NP_001986.2, NM_001995.3 [P33121-1]
XP_005262885.1, XM_005262828.1 [P33121-1]
XP_005262886.1, XM_005262829.1 [P33121-1]
XP_005262888.1, XM_005262831.1 [P33121-1]
XP_011530044.1, XM_011531742.1 [P33121-1]
XP_016863376.1, XM_017007887.1 [P33121-1]
XP_016863377.1, XM_017007888.1 [P33121-3]
XP_016863378.1, XM_017007889.1 [P33121-3]
UniGeneiHs.406678

Genome annotation databases

EnsembliENST00000281455; ENSP00000281455; ENSG00000151726 [P33121-1]
ENST00000504342; ENSP00000425006; ENSG00000151726 [P33121-1]
ENST00000513317; ENSP00000426150; ENSG00000151726 [P33121-3]
ENST00000515030; ENSP00000422607; ENSG00000151726 [P33121-1]
GeneIDi2180
KEGGihsa:2180
UCSCiuc003iwt.2 human [P33121-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiACSL1_HUMAN
AccessioniPrimary (citable) accession number: P33121
Secondary accession number(s): B7Z452
, D3DP57, P41215, Q8N8V7, Q8TA99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 18, 2018
This is version 174 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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