UniProtKB - P33025 (PSUG_ECOLI)
Protein
Pseudouridine-5'-phosphate glycosidase
Gene
psuG
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the reversible cleavage of pseudouridine 5'-phosphate (PsiMP) to ribose 5-phosphate and uracil. Functions biologically in the cleavage direction, as part of a pseudouridine degradation pathway.UniRule annotation2 Publications
Catalytic activityi
- EC:4.2.1.70UniRule annotation2 Publications
Cofactori
Mn2+UniRule annotation2 Publications, Fe2+2 Publications, Co2+2 PublicationsNote: Binds 1 manganese ion per subunit. Can also use Fe2+ and Co2+. The unusual metal binding site is heavily hydrated, coordinated with an aspartate side chain and five water molecules, and likely plays a role in anchoring the PsiMP phosphate.2 Publications
Activity regulationi
Inhibited by Zn2+ and Ni2+.1 Publication
Kineticsi
kcat is 3.74 sec(-1) for the synthesis of PsiMP.1 Publication
- KM=60 µM for pseudouridine 5'-phosphate (in the presence of 0.5 mM Mn2+)1 Publication
- KM=169.6 µM for uracil1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 31 | Proton donor1 PublicationUniRule annotation | 1 | |
Binding sitei | 93 | SubstrateUniRule annotation1 Publication | 1 | |
Binding sitei | 113 | Substrate; via amide nitrogenUniRule annotation1 Publication | 1 | |
Metal bindingi | 145 | ManganeseUniRule annotation1 Publication | 1 | |
Active sitei | 166 | Nucleophile1 PublicationUniRule annotation | 1 |
GO - Molecular functioni
- hydrolase activity, acting on glycosyl bonds Source: EcoliWiki
- identical protein binding Source: EcoCyc
- manganese ion binding Source: EcoCyc
- pseudouridylate synthase activity Source: EcoCyc
GO - Biological processi
- nucleobase catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Glycosidase, Hydrolase, Lyase |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12033-MONOMER MetaCyc:EG12033-MONOMER |
BRENDAi | 4.2.1.70, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Pseudouridine-5'-phosphate glycosidase1 PublicationUniRule annotation (EC:4.2.1.70UniRule annotation2 Publications)Short name: PsiMP glycosidase1 PublicationUniRule annotation |
Gene namesi | Name:psuGUniRule annotation Synonyms:pscG, yeiN1 Publication Ordered Locus Names:b2165, JW2152 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 31 | E → A: 7500-fold decrease in reaction rate while little change in substrate affinity. 1 Publication | 1 | |
Mutagenesisi | 93 | K → A: 17-fold decrease in reaction rate while modest decrease in substrate affinity. 1 Publication | 1 | |
Mutagenesisi | 149 | D → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 166 | K → A: 2900-fold decrease in reaction rate while no change in substrate affinity. 1 Publication | 1 | |
Mutagenesisi | 289 | N → A: 17-fold decrease in reaction rate while modest decrease in substrate affinity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169150 | 1 – 312 | Pseudouridine-5'-phosphate glycosidaseAdd BLAST | 312 |
Proteomic databases
jPOSTi | P33025 |
PaxDbi | P33025 |
PRIDEi | P33025 |
Interactioni
Subunit structurei
Homotrimer.
UniRule annotation1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261702, 6 interactors |
DIPi | DIP-11926N |
IntActi | P33025, 13 interactors |
STRINGi | 511145.b2165 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P33025 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 147 – 149 | Substrate bindingUniRule annotation1 Publication | 3 |
Sequence similaritiesi
Belongs to the pseudouridine-5'-phosphate glycosidase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG2313, Bacteria |
HOGENOMi | CLU_012201_0_1_6 |
InParanoidi | P33025 |
PhylomeDBi | P33025 |
Family and domain databases
Gene3Di | 3.40.1790.10, 1 hit |
HAMAPi | MF_01876, PsiMP_glycosidase, 1 hit |
InterProi | View protein in InterPro IPR022830, Indigdn_synthA-like IPR007342, PsuG |
PANTHERi | PTHR42909, PTHR42909, 1 hit |
Pfami | View protein in Pfam PF04227, Indigoidine_A, 1 hit |
SUPFAMi | SSF110581, SSF110581, 1 hit |
i Sequence
Sequence statusi: Complete.
P33025-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSELKISPEL LQISPEVQDA LKNKKPVVAL ESTIISHGMP FPQNAQTAIE
60 70 80 90 100
VEETIRKQGA VPATIAIIGG VMKVGLSKEE IELLGREGHN VTKVSRRDLP
110 120 130 140 150
FVVAAGKNGA TTVASTMIIA ALAGIKVFAT GGIGGVHRGA EHTFDISADL
160 170 180 190 200
QELANTNVTV VCAGAKSILD LGLTTEYLET FGVPLIGYQT KALPAFFCRT
210 220 230 240 250
SPFDVSIRLD SASEIARAMV VKWQSGLNGG LVVANPIPEQ FAMPEHTINA
260 270 280 290 300
AIDQAVAEAE AQGVIGKEST PFLLARVAEL TGGDSLKSNI QLVFNNAILA
310
SEIAKEYQRL AG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00007 Genomic DNA Translation: AAA60517.1 U00096 Genomic DNA Translation: AAC75226.1 AP009048 Genomic DNA Translation: BAE76642.1 |
PIRi | D64985 |
RefSeqi | NP_416670.1, NC_000913.3 WP_001292460.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75226; AAC75226; b2165 BAE76642; BAE76642; BAE76642 |
GeneIDi | 946699 |
KEGGi | ecj:JW2152 eco:b2165 |
PATRICi | fig|1411691.4.peg.74 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00007 Genomic DNA Translation: AAA60517.1 U00096 Genomic DNA Translation: AAC75226.1 AP009048 Genomic DNA Translation: BAE76642.1 |
PIRi | D64985 |
RefSeqi | NP_416670.1, NC_000913.3 WP_001292460.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4GIJ | X-ray | 1.94 | A/B/C | 1-312 | [»] | |
4GIK | X-ray | 2.19 | A/B/C | 1-312 | [»] | |
4GIL | X-ray | 2.54 | A/B/C | 1-312 | [»] | |
4GIM | X-ray | 1.80 | A/B/C | 1-312 | [»] | |
SMRi | P33025 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261702, 6 interactors |
DIPi | DIP-11926N |
IntActi | P33025, 13 interactors |
STRINGi | 511145.b2165 |
Proteomic databases
jPOSTi | P33025 |
PaxDbi | P33025 |
PRIDEi | P33025 |
Genome annotation databases
EnsemblBacteriai | AAC75226; AAC75226; b2165 BAE76642; BAE76642; BAE76642 |
GeneIDi | 946699 |
KEGGi | ecj:JW2152 eco:b2165 |
PATRICi | fig|1411691.4.peg.74 |
Organism-specific databases
EchoBASEi | EB1968 |
Phylogenomic databases
eggNOGi | COG2313, Bacteria |
HOGENOMi | CLU_012201_0_1_6 |
InParanoidi | P33025 |
PhylomeDBi | P33025 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12033-MONOMER MetaCyc:EG12033-MONOMER |
BRENDAi | 4.2.1.70, 2026 |
Miscellaneous databases
PROi | PR:P33025 |
Family and domain databases
Gene3Di | 3.40.1790.10, 1 hit |
HAMAPi | MF_01876, PsiMP_glycosidase, 1 hit |
InterProi | View protein in InterPro IPR022830, Indigdn_synthA-like IPR007342, PsuG |
PANTHERi | PTHR42909, PTHR42909, 1 hit |
Pfami | View protein in Pfam PF04227, Indigoidine_A, 1 hit |
SUPFAMi | SSF110581, SSF110581, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSUG_ECOLI | |
Accessioni | P33025Primary (citable) accession number: P33025 Secondary accession number(s): Q2MAR4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries