UniProtKB - P32960 (RACX_BACSU)
Protein
Broad specificity amino-acid racemase RacX
Gene
racX
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Amino-acid racemase able to utilize a broad range of substrates. Preferentially catalyzes the epimerization of LL-diaminopimelate, as well as the racemization of D-lysine, L-arginine, L-ornithine, L-lysine and D-arginine. Has lower activity against D-ornithine, L-histidine, L-alanine, L-tyrosine, L-phenylalanine, L-serine, L-glutamine, L-methionine, L-asparagine and L-homoserine. Has weak activity against L-norleucine, L-aminobutyric acid and L-norvaline. Has no activity toward nine L-amino acids (Thr, Glu, Asp, Val, Leu, Ile, Trp, Cit and Aad) (PubMed:28894939). D-amino acids might be used as components of peptidoglycan and/or be involved in peptidoglycan metabolism and remodeling (PubMed:8491712, PubMed:28894939).2 Publications
Catalytic activityi
- EC:5.1.1.101 Publication
Kineticsi
kcat is 0.08 min(-1) with L-lysine as substrate. kcat is 0.224 min(-1) with D-lysine as substrate.1 Publication
- KM=27.9 mM for L-lysine1 Publication
- KM=69.3 mM for D-lysine1 Publication
- Vmax=2.99 nmol/min/mg enzyme with L-lysine as substrate1 Publication
- Vmax=8.42 nmol/min/mg enzyme with D-lysine as substrate1 Publication
pH dependencei
Optimum pH is 8.5 with L-lysine as substrate.1 Publication
Temperature dependencei
Optimum temperature is 37 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 82 | Proton donor/acceptorBy similarity | 1 | |
Binding sitei | 161 | SubstrateBy similarity | 1 | |
Active sitei | 191 | Proton donor/acceptorBy similarity | 1 |
GO - Molecular functioni
- amino-acid racemase activity Source: UniProtKB-EC
GO - Biological processi
- nitrogen compound metabolic process Source: InterPro
- sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Keywordsi
Molecular function | Isomerase |
Biological process | Sporulation |
Enzyme and pathway databases
BioCyci | BSUB:BSU34430-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:racX Ordered Locus Names:BSU34430 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000095536 | 1 – 227 | Broad specificity amino-acid racemase RacXAdd BLAST | 227 |
Proteomic databases
PaxDbi | P32960 |
PRIDEi | P32960 |
Expressioni
Developmental stagei
Weakly expressed during vegetative growth and significantly induced at the onset of sporulation.1 Publication
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 224308.BSU34430 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 51 – 53 | Substrate bindingBy similarity | 3 | |
Regioni | 83 – 85 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the aspartate/glutamate racemases family.Curated
Phylogenomic databases
eggNOGi | COG1794, Bacteria |
InParanoidi | P32960 |
OMAi | SFIAMPC |
PhylomeDBi | P32960 |
Family and domain databases
InterProi | View protein in InterPro IPR015942, Asp/Glu/hydantoin_racemase IPR001920, Asp/Glu_race IPR018187, Asp/Glu_racemase_AS_1 IPR033134, Asp/Glu_racemase_AS_2 IPR004380, Asp_race |
Pfami | View protein in Pfam PF01177, Asp_Glu_race, 1 hit |
SUPFAMi | SSF53681, SSF53681, 2 hits |
TIGRFAMsi | TIGR00035, asp_race, 1 hit |
PROSITEi | View protein in PROSITE PS00923, ASP_GLU_RACEMASE_1, 1 hit PS00924, ASP_GLU_RACEMASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P32960-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIGILAGMGP KSTSPFIDKV IDYCQKLYGA SNDIDYPHMM IYSCPTPFYA
60 70 80 90 100
DRPIDHDEMK KAIIDGAVKL EKTGVDFIAL PCNTAHVYYE EIQQALSVPM
110 120 130 140 150
LHIVEETIKE IPHPAKKAVV LGTEPTIQSA IYQKVLKGNG QEVIHKDHWQ
160 170 180 190 200
QAVNQLIAAI KQPNHMQHTQ ALWQTLYEEI SQHADIIISA CTDLNAVLDH
210 220
IQSEIPIIDS SACLAKSTVS TYLAYQS
Sequence cautioni
The sequence AAA22641 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10629 Genomic DNA Translation: AAA22641.1 Different initiation. Z94043 Genomic DNA Translation: CAB08017.1 AL009126 Genomic DNA Translation: CAB15448.1 |
PIRi | B36908 |
RefSeqi | NP_391323.1, NC_000964.3 WP_003244427.1, NZ_JNCM01000033.1 |
Genome annotation databases
EnsemblBacteriai | CAB15448; CAB15448; BSU34430 |
GeneIDi | 938575 |
KEGGi | bsu:BSU34430 |
PATRICi | fig|224308.179.peg.3730 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L10629 Genomic DNA Translation: AAA22641.1 Different initiation. Z94043 Genomic DNA Translation: CAB08017.1 AL009126 Genomic DNA Translation: CAB15448.1 |
PIRi | B36908 |
RefSeqi | NP_391323.1, NC_000964.3 WP_003244427.1, NZ_JNCM01000033.1 |
3D structure databases
SMRi | P32960 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU34430 |
Proteomic databases
PaxDbi | P32960 |
PRIDEi | P32960 |
Genome annotation databases
EnsemblBacteriai | CAB15448; CAB15448; BSU34430 |
GeneIDi | 938575 |
KEGGi | bsu:BSU34430 |
PATRICi | fig|224308.179.peg.3730 |
Phylogenomic databases
eggNOGi | COG1794, Bacteria |
InParanoidi | P32960 |
OMAi | SFIAMPC |
PhylomeDBi | P32960 |
Enzyme and pathway databases
BioCyci | BSUB:BSU34430-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR015942, Asp/Glu/hydantoin_racemase IPR001920, Asp/Glu_race IPR018187, Asp/Glu_racemase_AS_1 IPR033134, Asp/Glu_racemase_AS_2 IPR004380, Asp_race |
Pfami | View protein in Pfam PF01177, Asp_Glu_race, 1 hit |
SUPFAMi | SSF53681, SSF53681, 2 hits |
TIGRFAMsi | TIGR00035, asp_race, 1 hit |
PROSITEi | View protein in PROSITE PS00923, ASP_GLU_RACEMASE_1, 1 hit PS00924, ASP_GLU_RACEMASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RACX_BACSU | |
Accessioni | P32960Primary (citable) accession number: P32960 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | April 14, 2009 | |
Last modified: | December 2, 2020 | |
This is version 124 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families