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Protein

Nucleoprotein

Gene

N

Organism
Avian infectious bronchitis virus (strain Gray) (IBV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding, Viral nucleoprotein
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NucleoproteinUniRule annotation
Alternative name(s):
Nucleocapsid proteinUniRule annotation
Short name:
NCUniRule annotation
Short name:
Protein NUniRule annotation
Gene namesi
Name:NUniRule annotation
ORF Names:6
OrganismiAvian infectious bronchitis virus (strain Gray) (IBV)
Taxonomic identifieri31624 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeGammacoronavirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host Golgi apparatus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001059811 – 409NucleoproteinAdd BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei190Phosphoserine; by hostUniRule annotation1
Modified residuei192Phosphoserine; by hostUniRule annotation1
Disulfide bondi320 ↔ 323UniRule annotation
Modified residuei378Phosphothreonine; by hostUniRule annotation1
Modified residuei379Phosphoserine; by hostUniRule annotation1

Post-translational modificationi

ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.UniRule annotation
Phosphorylated on serine and threonine residues.UniRule annotation

Keywords - PTMi

ADP-ribosylation, Disulfide bond, Phosphoprotein

Proteomic databases

PRIDEiP32923

Interactioni

Subunit structurei

Homooligomer. Both monomeric and oligomeric forms interact with RNA. Interacts with protein M. Interacts with NSP3; this interaction serves to tether the genome to the newly translated replicase-transcriptase complex at a very early stage of infection.UniRule annotation

Structurei

Secondary structure

1409
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP32923
SMRiP32923
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32923

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 160RNA-bindingUniRule annotationAdd BLAST132
Regioni226 – 333DimerizationUniRule annotationAdd BLAST108

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi165 – 190Ser-richUniRule annotationAdd BLAST26

Sequence similaritiesi

Belongs to the gammacoronavirus nucleocapsid protein family.UniRule annotation

Family and domain databases

HAMAPiMF_04097 GAMMA_CORONA_NCAP, 1 hit
InterProiView protein in InterPro
IPR001218 Corona_nucleocap
IPR037179 Nucleocapsid_C
IPR037195 Nucleocapsid_N
PfamiView protein in Pfam
PF00937 Corona_nucleoca, 1 hit
PIRSFiPIRSF003888 Corona_nucleocap, 1 hit
SUPFAMiSSF103068 SSF103068, 1 hit
SSF110304 SSF110304, 1 hit

Sequencei

Sequence statusi: Complete.

P32923-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASGKATGKT DAPAPVIKLG GPRPPKVGSS GNASWFQAIK AKKLNSPQPK
60 70 80 90 100
FEGSGVPDNE NFKTSQQHGY WRRQARFKPG KGRRKPVPDA WYFYYTGTGP
110 120 130 140 150
AADLNWGDSQ DGIVWVAAKG ADVKSRSNQG TRDPDKFDQY PLRFSDGGPD
160 170 180 190 200
GNFRWDFIPL NRGRSGRSTA ASSAASSRPP SREGSRGRRS GSEDDLIARA
210 220 230 240 250
AKIIQDQQKK GSRITKAKAD EMAHRRYCKR TIPPGYKVDQ VFGPRTKGKE
260 270 280 290 300
GNFGDDKMNE EGIKDGRVTA MLNLVPSSHA CLFGSRVTPK LQPDGLHLKF
310 320 330 340 350
EFTTVVPRDD PQFDNYVKIC DQCVDGVGTR PKDDEPKPKS RSSSRPATRT
360 370 380 390 400
SSPAPRQQRL KKEKRPKKQD DEVDKALTSD EERNNAQLEF DDEPKVINWG

DSALGENEL
Length:409
Mass (Da):45,249
Last modified:June 1, 1994 - v2
Checksum:i5C15F07A0A60BE80
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223 – 243AHRRY…DQVFG → VIAGIASALFHLVIRLIKFL V (PubMed:1966426).Add BLAST21
Sequence conflicti245R → G (PubMed:1966426).1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S55229 mRNA No translation available.
S48137 Genomic RNA Translation: AAB24054.1
M85245 Genomic RNA Translation: AAA91856.1
PIRiA43574 VHIHG1
A48559

Similar proteinsi

Entry informationi

Entry nameiNCAP_IBVG
AccessioniPrimary (citable) accession number: P32923
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 1, 1994
Last modified: June 20, 2018
This is version 77 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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