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Protein

Tryptophan--tRNA ligase, cytoplasmic

Gene

Wars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

T1-TrpRS has aminoacylation activity while T2-TrpRS lacks it. T1-TrpRS and T2-TrpRS possess angiostatic activity. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (By similarity).By similarity

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processAngiogenesis, Protein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.1.1.2 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, cytoplasmic (EC:6.1.1.2By similarity)
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Cleaved into the following 2 chains:
Gene namesi
Name:Wars
Synonyms:Wrs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:104630 Wars

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001367391 – 481Tryptophan--tRNA ligase, cytoplasmicAdd BLAST481
ChainiPRO_000038646375 – 481T1-TrpRSBy similarityAdd BLAST407
ChainiPRO_000038646498 – 481T2-TrpRSBy similarityAdd BLAST384

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei158N6-succinyllysineCombined sources1
Modified residuei355PhosphoserineBy similarity1

Post-translational modificationi

Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32921
PaxDbiP32921
PeptideAtlasiP32921
PRIDEiP32921

PTM databases

iPTMnetiP32921
PhosphoSitePlusiP32921
SwissPalmiP32921

Expressioni

Tissue specificityi

Isoform 2 is widely expressed, isoform 1 is found only in embryonic stem cells.

Gene expression databases

BgeeiENSMUSG00000021266
CleanExiMM_WARS
ExpressionAtlasiP32921 baseline and differential
GenevisibleiP32921 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with oxidized form of GAPDH (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204543, 4 interactors
IntActiP32921, 2 interactors
MINTiP32921
STRINGi10090.ENSMUSP00000124625

Structurei

3D structure databases

ProteinModelPortaliP32921
SMRiP32921
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 68WHEP-TRSPROSITE-ProRule annotationAdd BLAST57

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi168 – 177"HIGH" region10
Motifi353 – 357"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2145 Eukaryota
COG0180 LUCA
GeneTreeiENSGT00390000000666
HOGENOMiHOG000224742
HOVERGENiHBG002325
InParanoidiP32921
KOiK01867
OMAiADILHPQ
OrthoDBiEOG091G09U9
PhylomeDBiP32921
TreeFamiTF105669

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR009068 S15_NS1_RNA-bd
IPR002306 Trp-tRNA-ligase
IPR000738 WHEP-TRS_dom
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PF00458 WHEP-TRS, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
SMARTiView protein in SMART
SM00991 WHEP-TRS, 1 hit
SUPFAMiSSF47060 SSF47060, 1 hit
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
PS00762 WHEP_TRS_1, 1 hit
PS51185 WHEP_TRS_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P32921-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADMPSGESC TSPLELFNSI ATQGELVRSL KAGNAPKDEI DSAVKMLLSL
60 70 80 90 100
KMSYKAAMGE EYKAGCPPGN PTAGRNCDSD ATKASEDFVD PWTVRTSSAK
110 120 130 140 150
GIDYDKLIVQ FGSSKIDKEL INRIERATGQ RPHRFLRRGI FFSHRDMNQI
160 170 180 190 200
LDAYENKKPF YLYTGRGPSS EAMHLGHLVP FIFTKWLQDV FNVPLVIQMS
210 220 230 240 250
DDEKYLWKDL TLEQAYSYTV ENAKDIIACG FDINKTFIFS DLEYMGQSPG
260 270 280 290 300
FYRNVVKIQK HVTFNQVKGI FGFTDSDCIG KISFPAVQAA PSFSNSFPKI
310 320 330 340 350
FRDRTDIQCL IPCAIDQDPY FRMTRDVAPR IGHPKPALLH STFFPALQGA
360 370 380 390 400
QTKMSASDPN SSIFLTDTAK QIKSKVNKHA FSGGRDTVEE HRQFGGNCEV
410 420 430 440 450
DVSFMYLTFF LEDDDRLEQI RKDYTSGAML TGELKKTLID VLQPLIAEHQ
460 470 480
ARRKAVTEET VKEFMTPRQL SFHFQCFCFD T
Length:481
Mass (Da):54,358
Last modified:August 31, 2004 - v2
Checksum:i39B55989AE895346
GO
Isoform 2 (identifier: P32921-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     476-481: Missing.

Show »
Length:475
Mass (Da):53,641
Checksum:iC3467FE85521DE4C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52M → V in AAH03450 (PubMed:15489334).Curated1
Sequence conflicti94V → A in AAH46232 (PubMed:15489334).Curated1
Sequence conflicti111F → P in CAA49347 (PubMed:7932716).Curated1
Sequence conflicti111F → P in CAA49348 (PubMed:7932716).Curated1
Sequence conflicti188Q → E in CAA49347 (PubMed:7932716).Curated1
Sequence conflicti188Q → E in CAA49348 (PubMed:7932716).Curated1
Sequence conflicti282I → S in CAA49347 (PubMed:7932716).Curated1
Sequence conflicti282I → S in CAA49348 (PubMed:7932716).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006313476 – 481Missing in isoform 2. 2 Publications6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69656 Genomic DNA Translation: CAA49347.1
X69657 Genomic DNA Translation: CAA49348.1
AK004541 mRNA Translation: BAB23357.1
BC003450 mRNA Translation: AAH03450.1
BC046232 mRNA Translation: AAH46232.1
CCDSiCCDS26166.1 [P32921-1]
CCDS49171.1 [P32921-2]
PIRiS50053
RefSeqiNP_001157786.1, NM_001164314.1 [P32921-2]
NP_001157960.1, NM_001164488.1 [P32921-2]
NP_035840.3, NM_011710.3 [P32921-1]
XP_006515882.1, XM_006515819.1 [P32921-1]
UniGeneiMm.38433

Genome annotation databases

EnsembliENSMUST00000109848; ENSMUSP00000105474; ENSMUSG00000021266 [P32921-2]
ENSMUST00000161154; ENSMUSP00000124625; ENSMUSG00000021266 [P32921-1]
ENSMUST00000161410; ENSMUSP00000125320; ENSMUSG00000021266 [P32921-2]
GeneIDi22375
KEGGimmu:22375
UCSCiuc007pag.2 mouse [P32921-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSYWC_MOUSE
AccessioniPrimary (citable) accession number: P32921
Secondary accession number(s): Q80ZY4, Q99J58, Q9DC65
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: August 31, 2004
Last modified: July 18, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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