UniProtKB - P32921 (SYWC_MOUSE)
Protein
Tryptophan--tRNA ligase, cytoplasmic
Gene
Wars1
Organism
Mus musculus (Mouse)
Status
Functioni
T1-TrpRS has aminoacylation activity while T2-TrpRS lacks it. T1-TrpRS and T2-TrpRS possess angiostatic activity. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression (By similarity).By similarity
Catalytic activityi
- EC:6.1.1.2By similarity
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kinase inhibitor activity Source: MGI
- protein domain specific binding Source: MGI
- protein homodimerization activity Source: UniProtKB
- protein kinase binding Source: MGI
- tryptophan-tRNA ligase activity Source: MGI
GO - Biological processi
- angiogenesis Source: UniProtKB-KW
- negative regulation of protein kinase activity Source: MGI
- negative regulation of protein phosphorylation Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of protein-containing complex assembly Source: MGI
- regulation of angiogenesis Source: MGI
- regulation of protein ADP-ribosylation Source: MGI
- tryptophanyl-tRNA aminoacylation Source: CAFA
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase |
Biological process | Angiogenesis, Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.1.1.2, 3474 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Wars1 Synonyms:Wars, Wrs |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104630, Wars |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: GO_Central
- protein-containing complex Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000136739 | 1 – 481 | Tryptophan--tRNA ligase, cytoplasmicAdd BLAST | 481 | |
ChainiPRO_0000386463 | 75 – 481 | T1-TrpRSBy similarityAdd BLAST | 407 | |
ChainiPRO_0000386464 | 98 – 481 | T2-TrpRSBy similarityAdd BLAST | 384 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 158 | N6-succinyllysineCombined sources | 1 | |
Modified residuei | 355 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P32921 |
MaxQBi | P32921 |
PaxDbi | P32921 |
PeptideAtlasi | P32921 |
PRIDEi | P32921 |
PTM databases
iPTMneti | P32921 |
PhosphoSitePlusi | P32921 |
SwissPalmi | P32921 |
Expressioni
Tissue specificityi
Isoform 2 is widely expressed, isoform 1 is found only in embryonic stem cells.
Gene expression databases
Bgeei | ENSMUSG00000021266, Expressed in prostate gland and 317 other tissues |
ExpressionAtlasi | P32921, baseline and differential |
Genevisiblei | P32921, MM |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with oxidized form of GAPDH (By similarity).
By similarityGO - Molecular functioni
- protein domain specific binding Source: MGI
- protein homodimerization activity Source: UniProtKB
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204543, 24 interactors |
IntActi | P32921, 2 interactors |
MINTi | P32921 |
STRINGi | 10090.ENSMUSP00000124625 |
Miscellaneous databases
RNActi | P32921, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 68 | WHEP-TRSPROSITE-ProRule annotationAdd BLAST | 57 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 168 – 177 | 'HIGH' region | 10 | |
Motifi | 353 – 357 | 'KMSKS' region | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG2145, Eukaryota |
GeneTreei | ENSGT00940000153724 |
HOGENOMi | CLU_032621_0_1_1 |
InParanoidi | P32921 |
OMAi | SIYHRFM |
OrthoDBi | 817008at2759 |
PhylomeDBi | P32921 |
TreeFami | TF105669 |
Family and domain databases
CDDi | cd00806, TrpRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002305, aa-tRNA-synth_Ic IPR014729, Rossmann-like_a/b/a_fold IPR009068, S15_NS1_RNA-bd IPR002306, Trp-tRNA-ligase IPR000738, WHEP-TRS_dom |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit PF00458, WHEP-TRS, 1 hit |
PRINTSi | PR01039, TRNASYNTHTRP |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit |
TIGRFAMsi | TIGR00233, trpS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P32921-1) [UniParc]FASTAAdd to basket
Also known as: Long
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADMPSGESC TSPLELFNSI ATQGELVRSL KAGNAPKDEI DSAVKMLLSL
60 70 80 90 100
KMSYKAAMGE EYKAGCPPGN PTAGRNCDSD ATKASEDFVD PWTVRTSSAK
110 120 130 140 150
GIDYDKLIVQ FGSSKIDKEL INRIERATGQ RPHRFLRRGI FFSHRDMNQI
160 170 180 190 200
LDAYENKKPF YLYTGRGPSS EAMHLGHLVP FIFTKWLQDV FNVPLVIQMS
210 220 230 240 250
DDEKYLWKDL TLEQAYSYTV ENAKDIIACG FDINKTFIFS DLEYMGQSPG
260 270 280 290 300
FYRNVVKIQK HVTFNQVKGI FGFTDSDCIG KISFPAVQAA PSFSNSFPKI
310 320 330 340 350
FRDRTDIQCL IPCAIDQDPY FRMTRDVAPR IGHPKPALLH STFFPALQGA
360 370 380 390 400
QTKMSASDPN SSIFLTDTAK QIKSKVNKHA FSGGRDTVEE HRQFGGNCEV
410 420 430 440 450
DVSFMYLTFF LEDDDRLEQI RKDYTSGAML TGELKKTLID VLQPLIAEHQ
460 470 480
ARRKAVTEET VKEFMTPRQL SFHFQCFCFD T
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE0CXQ4 | E0CXQ4_MOUSE | Tryptophanyl-tRNA synthetase | Wars | 59 | Annotation score: | ||
E0CXX4 | E0CXX4_MOUSE | Tryptophanyl-tRNA synthetase | Wars | 39 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 52 | M → V in AAH03450 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 94 | V → A in AAH46232 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 111 | F → P in CAA49347 (PubMed:7932716).Curated | 1 | |
Sequence conflicti | 111 | F → P in CAA49348 (PubMed:7932716).Curated | 1 | |
Sequence conflicti | 188 | Q → E in CAA49347 (PubMed:7932716).Curated | 1 | |
Sequence conflicti | 188 | Q → E in CAA49348 (PubMed:7932716).Curated | 1 | |
Sequence conflicti | 282 | I → S in CAA49347 (PubMed:7932716).Curated | 1 | |
Sequence conflicti | 282 | I → S in CAA49348 (PubMed:7932716).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006313 | 476 – 481 | Missing in isoform 2. 2 Publications | 6 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69656 Genomic DNA Translation: CAA49347.1 X69657 Genomic DNA Translation: CAA49348.1 AK004541 mRNA Translation: BAB23357.1 BC003450 mRNA Translation: AAH03450.1 BC046232 mRNA Translation: AAH46232.1 |
CCDSi | CCDS26166.1 [P32921-1] CCDS49171.1 [P32921-2] |
PIRi | S50053 |
RefSeqi | NP_001157786.1, NM_001164314.1 [P32921-2] NP_001157960.1, NM_001164488.1 [P32921-2] NP_035840.3, NM_011710.3 [P32921-1] XP_006515882.1, XM_006515819.1 [P32921-1] |
Genome annotation databases
Ensembli | ENSMUST00000109848; ENSMUSP00000105474; ENSMUSG00000021266 [P32921-2] ENSMUST00000161154; ENSMUSP00000124625; ENSMUSG00000021266 [P32921-1] ENSMUST00000161410; ENSMUSP00000125320; ENSMUSG00000021266 [P32921-2] |
GeneIDi | 22375 |
KEGGi | mmu:22375 |
UCSCi | uc007pag.2, mouse [P32921-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X69656 Genomic DNA Translation: CAA49347.1 X69657 Genomic DNA Translation: CAA49348.1 AK004541 mRNA Translation: BAB23357.1 BC003450 mRNA Translation: AAH03450.1 BC046232 mRNA Translation: AAH46232.1 |
CCDSi | CCDS26166.1 [P32921-1] CCDS49171.1 [P32921-2] |
PIRi | S50053 |
RefSeqi | NP_001157786.1, NM_001164314.1 [P32921-2] NP_001157960.1, NM_001164488.1 [P32921-2] NP_035840.3, NM_011710.3 [P32921-1] XP_006515882.1, XM_006515819.1 [P32921-1] |
3D structure databases
SMRi | P32921 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204543, 24 interactors |
IntActi | P32921, 2 interactors |
MINTi | P32921 |
STRINGi | 10090.ENSMUSP00000124625 |
PTM databases
iPTMneti | P32921 |
PhosphoSitePlusi | P32921 |
SwissPalmi | P32921 |
Proteomic databases
jPOSTi | P32921 |
MaxQBi | P32921 |
PaxDbi | P32921 |
PeptideAtlasi | P32921 |
PRIDEi | P32921 |
Protocols and materials databases
Antibodypediai | 27522, 242 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000109848; ENSMUSP00000105474; ENSMUSG00000021266 [P32921-2] ENSMUST00000161154; ENSMUSP00000124625; ENSMUSG00000021266 [P32921-1] ENSMUST00000161410; ENSMUSP00000125320; ENSMUSG00000021266 [P32921-2] |
GeneIDi | 22375 |
KEGGi | mmu:22375 |
UCSCi | uc007pag.2, mouse [P32921-1] |
Organism-specific databases
CTDi | 22375 |
MGIi | MGI:104630, Wars |
Phylogenomic databases
eggNOGi | KOG2145, Eukaryota |
GeneTreei | ENSGT00940000153724 |
HOGENOMi | CLU_032621_0_1_1 |
InParanoidi | P32921 |
OMAi | SIYHRFM |
OrthoDBi | 817008at2759 |
PhylomeDBi | P32921 |
TreeFami | TF105669 |
Enzyme and pathway databases
BRENDAi | 6.1.1.2, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 22375, 9 hits in 20 CRISPR screens |
ChiTaRSi | Wars, mouse |
PROi | PR:P32921 |
RNActi | P32921, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021266, Expressed in prostate gland and 317 other tissues |
ExpressionAtlasi | P32921, baseline and differential |
Genevisiblei | P32921, MM |
Family and domain databases
CDDi | cd00806, TrpRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002305, aa-tRNA-synth_Ic IPR014729, Rossmann-like_a/b/a_fold IPR009068, S15_NS1_RNA-bd IPR002306, Trp-tRNA-ligase IPR000738, WHEP-TRS_dom |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit PF00458, WHEP-TRS, 1 hit |
PRINTSi | PR01039, TRNASYNTHTRP |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit |
TIGRFAMsi | TIGR00233, trpS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYWC_MOUSE | |
Accessioni | P32921Primary (citable) accession number: P32921 Secondary accession number(s): Q80ZY4, Q99J58, Q9DC65 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | August 31, 2004 | |
Last modified: | December 2, 2020 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families