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Protein

Protein transport protein SEC61

Gene

SEC61

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the Sec61 complex, which is the major component of a channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). The functional states of the translocon complex include co- and post-translational ER import, cotranslational membrane protein integration and retrograde transport of misfolded proteins out of the ER. In the cotranslational pathway, ribosomes synthesizing presecretory proteins are targeted to the translocon by the cytosolic signal recognition particle (SRP) and its ER-localized receptor. The association of the Sec61 complex with the ribosome is mediated by the 28S rRNA of the large ribosomal subunit. SRP-independent post-translational translocation requires the association of additional factors, such as the Sec62/63 complex and KAR2. In an initial step, the signal sequence seems to bind simultaneously to SEC61 and SEC62. A cycle of assembly and disassembly of Sec62/63 complex from SEC61 may govern the activity of the translocon. SEC61 mediates the association with the ribosome.

Miscellaneous

Present with 24800 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • peptide transmembrane transporter activity Source: SGD
  • P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: SGD
  • protein transmembrane transporter activity Source: GO_Central
  • protein transporter activity Source: SGD
  • signal sequence binding Source: SGD

GO - Biological processi

Keywordsi

Biological processProtein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32445-MONOMER
ReactomeiR-SCE-381038 XBP1(S) activates chaperone genes

Protein family/group databases

TCDBi3.A.5.8.1 the general secretory pathway (sec) family

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein SEC61
Alternative name(s):
Sec61 complex subunit SEC61
Sec61 complex subunit alpha
Gene namesi
Name:SEC61
Ordered Locus Names:YLR378C
ORF Names:L3502.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

SGDiS000004370 SEC61

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32CytoplasmicCuratedAdd BLAST32
Transmembranei33 – 55Helical; Name=1CuratedAdd BLAST23
Topological domaini56 – 75LumenalCuratedAdd BLAST20
Transmembranei76 – 95Helical; Name=2CuratedAdd BLAST20
Topological domaini96 – 119CytoplasmicCuratedAdd BLAST24
Transmembranei120 – 141Helical; Name=3CuratedAdd BLAST22
Topological domaini142 – 146LumenalCurated5
Transmembranei147 – 167Helical; Name=4CuratedAdd BLAST21
Topological domaini168 – 212CytoplasmicCuratedAdd BLAST45
Transmembranei213 – 224Helical; Name=5CuratedAdd BLAST12
Topological domaini225 – 240LumenalCuratedAdd BLAST16
Transmembranei241 – 260Helical; Name=6CuratedAdd BLAST20
Topological domaini261 – 290CytoplasmicCuratedAdd BLAST30
Transmembranei291 – 311Helical; Name=7CuratedAdd BLAST21
Topological domaini312 – 361LumenalCuratedAdd BLAST50
Transmembranei362 – 381Helical; Name=8CuratedAdd BLAST20
Topological domaini382 – 416CytoplasmicCuratedAdd BLAST35
Transmembranei417 – 434Helical; Name=9CuratedAdd BLAST18
Topological domaini435 – 437LumenalCurated3
Transmembranei438 – 459Helical; Name=10CuratedAdd BLAST22
Topological domaini460 – 480CytoplasmicCuratedAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi273K → P or G: Severe growth defect. 1 Publication1
Mutagenesisi275R → D, G, P, Q or Y: Severe growth defect. 1 Publication1
Mutagenesisi275R → E, F or V: Severe growth defect; lowers SRP-dependent and SRP-independent translocation. 1 Publication1
Mutagenesisi276G → P: Severe growth defect. 1 Publication1
Mutagenesisi405K → D, E or P: Severe growth defect. 1 Publication1
Mutagenesisi406R → D: Severe growth defect; lowers SRP-dependent translocation. 1 Publication1
Mutagenesisi406R → E: Severe growth defect; lowers SRP-dependent and SRP-independent translocation. 1 Publication1
Mutagenesisi406R → H or W: Severe growth defect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001317891 – 480Protein transport protein SEC61Add BLAST480

Proteomic databases

MaxQBiP32915
PaxDbiP32915
PRIDEiP32915
TopDownProteomicsiP32915

Interactioni

Subunit structurei

Component of the heterotrimeric Sec61 complex, which is composed of SEC61, SBH1 and SSS1. Presumably three to four Sec61 heterotrimers assemble into an oligomeric ring with a central aqueous pore. In cotranslational ER import, the pore diameter varies from 9-15 A in a ribosome-free resting state to 40-60 A in a functional state when associated with the ribosome. The Sec61 complex is part of a channel-forming translocon complex whose composition seem to change dependent upon different functional states. During post-translational ER import the Sec61 complex associates with the Sec62/63 complex to form the Sec complex. SEC61 interacts with STT3, OST1, OST2, OST4, SWP1 AND WBP1 components of the OT complex.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi31638, 470 interactors
ComplexPortaliCPX-1833 SEC61 translocon complex
CPX-3055 Translocon complex
DIPiDIP-2433N
IntActiP32915, 20 interactors
MINTiP32915
STRINGi4932.YLR378C

Structurei

3D structure databases

ProteinModelPortaliP32915
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SecY/SEC61-alpha family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000003721
HOGENOMiHOG000231249
InParanoidiP32915
KOiK10956
OMAiKWGIGSG
OrthoDBiEOG092C21L2

Family and domain databases

Gene3Di1.10.3370.10, 1 hit
InterProiView protein in InterPro
IPR002208 SecY/SEC61-alpha
IPR030659 SecY_CS
IPR023201 SecY_dom_sf
IPR019561 Translocon_Sec61/SecY_plug_dom
PANTHERiPTHR10906 PTHR10906, 1 hit
PfamiView protein in Pfam
PF10559 Plug_translocon, 1 hit
PF00344 SecY, 1 hit
PIRSFiPIRSF004557 SecY, 1 hit
SUPFAMiSSF103491 SSF103491, 1 hit
TIGRFAMsiTIGR00967 3a0501s007, 1 hit
PROSITEiView protein in PROSITE
PS00755 SECY_1, 1 hit
PS00756 SECY_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32915-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSNRVLDLF KPFESFLPEV IAPERKVPYN QKLIWTGVSL LIFLILGQIP
60 70 80 90 100
LYGIVSSETS DPLYWLRAML ASNRGTLLEL GVSPIITSSM IFQFLQGTQL
110 120 130 140 150
LQIRPESKQD RELFQIAQKV CAIILILGQA LVVVMTGNYG APSDLGLPIC
160 170 180 190 200
LLLIFQLMFA SLIVMLLDEL LSKGYGLGSG ISLFTATNIA EQIFWRAFAP
210 220 230 240 250
TTVNSGRGKE FEGAVIAFFH LLAVRKDKKR ALVEAFYRTN LPNMFQVLMT
260 270 280 290 300
VAIFLFVLYL QGFRYELPIR STKVRGQIGI YPIKLFYTSN TPIMLQSALT
310 320 330 340 350
SNIFLISQIL FQKYPTNPLI RLIGVWGIRP GTQGPQMALS GLAYYIQPLM
360 370 380 390 400
SLSEALLDPI KTIVYITFVL GSCAVFSKTW IEISGTSPRD IAKQFKDQGM
410 420 430 440 450
VINGKRETSI YRELKKIIPT AAAFGGATIG ALSVGSDLLG TLGSGASILM
460 470 480
ATTTIYGYYE AAAKEGGFTK NLVPGFSDLM
Length:480
Mass (Da):52,937
Last modified:October 1, 1993 - v1
Checksum:iAB34F12FB7B3CBD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62340 Genomic DNA Translation: CAA44215.1
U19104 Genomic DNA Translation: AAB67276.1
U19103 Genomic DNA Translation: AAB67581.1
BK006945 Genomic DNA Translation: DAA09680.1
PIRiA60043
RefSeqiNP_013482.1, NM_001182267.1

Genome annotation databases

EnsemblFungiiYLR378C; YLR378C; YLR378C
GeneIDi851095
KEGGisce:YLR378C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62340 Genomic DNA Translation: CAA44215.1
U19104 Genomic DNA Translation: AAB67276.1
U19103 Genomic DNA Translation: AAB67581.1
BK006945 Genomic DNA Translation: DAA09680.1
PIRiA60043
RefSeqiNP_013482.1, NM_001182267.1

3D structure databases

ProteinModelPortaliP32915
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31638, 470 interactors
ComplexPortaliCPX-1833 SEC61 translocon complex
CPX-3055 Translocon complex
DIPiDIP-2433N
IntActiP32915, 20 interactors
MINTiP32915
STRINGi4932.YLR378C

Protein family/group databases

TCDBi3.A.5.8.1 the general secretory pathway (sec) family

Proteomic databases

MaxQBiP32915
PaxDbiP32915
PRIDEiP32915
TopDownProteomicsiP32915

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR378C; YLR378C; YLR378C
GeneIDi851095
KEGGisce:YLR378C

Organism-specific databases

SGDiS000004370 SEC61

Phylogenomic databases

GeneTreeiENSGT00390000003721
HOGENOMiHOG000231249
InParanoidiP32915
KOiK10956
OMAiKWGIGSG
OrthoDBiEOG092C21L2

Enzyme and pathway databases

BioCyciYEAST:G3O-32445-MONOMER
ReactomeiR-SCE-381038 XBP1(S) activates chaperone genes

Miscellaneous databases

PROiPR:P32915

Family and domain databases

Gene3Di1.10.3370.10, 1 hit
InterProiView protein in InterPro
IPR002208 SecY/SEC61-alpha
IPR030659 SecY_CS
IPR023201 SecY_dom_sf
IPR019561 Translocon_Sec61/SecY_plug_dom
PANTHERiPTHR10906 PTHR10906, 1 hit
PfamiView protein in Pfam
PF10559 Plug_translocon, 1 hit
PF00344 SecY, 1 hit
PIRSFiPIRSF004557 SecY, 1 hit
SUPFAMiSSF103491 SSF103491, 1 hit
TIGRFAMsiTIGR00967 3a0501s007, 1 hit
PROSITEiView protein in PROSITE
PS00755 SECY_1, 1 hit
PS00756 SECY_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSC61A_YEAST
AccessioniPrimary (citable) accession number: P32915
Secondary accession number(s): D6VZ14
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 7, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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Main funding by: National Institutes of Health

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