We will be switching to the new UniProt website on Tuesday, June 28. Please explore and share your feedback.
Take me to UniProt BETA
UniProtKB - P32912 (VAM7_YEAST)
Protein
Vacuolar morphogenesis protein 7
Gene
VAM7
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Essential for proper morphogenesis of the vacuole. May exist as structural reinforcement on the surface of the vacuolar membrane and be required for maintenance against rupture by osmotic pressure.
Miscellaneous
Present with 2360 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- phosphatidylinositol-3-phosphate binding Source: SGD
- SNAP receptor activity Source: SGD
- SNARE binding Source: GO_Central
GO - Biological processi
- amphisome-lysosome fusion Source: ComplexPortal
- autophagosome-lysosome fusion Source: SGD
- cytoplasm to vacuole transport by the Cvt pathway Source: SGD
- intracellular protein transport Source: GO_Central
- macroautophagy Source: SGD
- piecemeal microautophagy of the nucleus Source: SGD
- vacuolar calcium ion homeostasis Source: ComplexPortal
- vacuole fusion, non-autophagic Source: SGD
- vesicle docking Source: GO_Central
- vesicle fusion Source: SGD
Protein family/group databases
TCDBi | 1.F.1.1.2, the synaptosomal vesicle fusion pore (svf-pore) family |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar morphogenesis protein 7 |
Gene namesi | Name:VAM7 Ordered Locus Names:YGL212W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003180, VAM7 |
VEuPathDBi | FungiDB:YGL212W |
Subcellular locationi
Vacuole
Vacuole
- autophagosome membrane Source: ComplexPortal
- fungal-type vacuole membrane Source: SGD
- vacuolar membrane Source: ComplexPortal
Other locations
- endomembrane system Source: GO_Central
- integral component of membrane Source: GO_Central
- phagophore assembly site Source: SGD
- SNARE complex Source: SGD
Keywords - Cellular componenti
VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000065760 | 1 – 316 | Vacuolar morphogenesis protein 7Add BLAST | 316 |
Proteomic databases
MaxQBi | P32912 |
PaxDbi | P32912 |
PRIDEi | P32912 |
PTM databases
iPTMneti | P32912 |
Interactioni
Subunit structurei
Possibly multimeric. Associates with VAM3.
Binary interactionsi
P32912
With | #Exp. | IntAct |
---|---|---|
NYV1 [Q12255] | 11 | EBI-20232,EBI-35465 |
RBD2 [Q12270] | 3 | EBI-20232,EBI-31471 |
VAM3 [Q12241] | 11 | EBI-20232,EBI-20227 |
VPS33 [P20795] | 4 | EBI-20232,EBI-20395 |
YIF1 [P53845] | 3 | EBI-20232,EBI-28230 |
GO - Molecular functioni
- SNARE binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 33044, 709 interactors |
ComplexPortali | CPX-1887, Vacuolar SNARE complex VAM3-VTI1-VAM7-YKT6 CPX-5401, Vacuolar SNARE complex VAM3-VTI1-VAM7-NYV1 |
DIPi | DIP-1722N |
IntActi | P32912, 36 interactors |
MINTi | P32912 |
STRINGi | 4932.YGL212W |
Miscellaneous databases
RNActi | P32912, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P32912 |
SMRi | P32912 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32912 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 124 | PXPROSITE-ProRule annotationAdd BLAST | 124 | |
Domaini | 250 – 312 | t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST | 63 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 168 – 186 | Sequence analysisAdd BLAST | 19 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3202, Eukaryota |
HOGENOMi | CLU_033748_1_0_1 |
InParanoidi | P32912 |
OMAi | NITMMRI |
Family and domain databases
Gene3Di | 3.30.1520.10, 1 hit |
InterProi | View protein in InterPro IPR001683, PX_dom IPR036871, PX_dom_sf IPR045242, Syntaxin IPR000727, T_SNARE_dom |
PANTHERi | PTHR19957, PTHR19957, 1 hit |
Pfami | View protein in Pfam PF00787, PX, 1 hit |
SMARTi | View protein in SMART SM00312, PX, 1 hit SM00397, t_SNARE, 1 hit |
SUPFAMi | SSF64268, SSF64268, 1 hit |
PROSITEi | View protein in PROSITE PS50195, PX, 1 hit PS50192, T_SNARE, 1 hit |
i Sequence
Sequence statusi: Complete.
P32912-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAANSVGKMS EKLRIKVDDV KINPKYVLYG VSTPNKRLYK RYSEFWKLKT
60 70 80 90 100
RLERDVGSTI PYDFPEKPGV LDRRWQRRYD DPEMIDERRI GLERFLNELY
110 120 130 140 150
NDRFDSRWRD TKIAQDFLQL SKPNVSQEKS QQHLETADEV GWDEMIRDIK
160 170 180 190 200
LDLDKESDGT PSVRGALRAR TKLHKLRERL EQDVQKKSLP STEVTRRAAL
210 220 230 240 250
LRSLLKECDD IGTANIAQDR GRLLGVATSD NSSTTEVQGR TNNDLQQGQM
260 270 280 290 300
QMVRDQEQEL VALHRIIQAQ RGLALEMNEE LQTQNELLTA LEDDVDNTGR
310
RLQIANKKAR HFNNSA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D11379 Genomic DNA Translation: BAA01977.1 U33754 Genomic DNA Translation: AAC49494.1 Z72734 Genomic DNA Translation: CAA96928.1 BK006941 Genomic DNA Translation: DAA07904.1 |
PIRi | S31263 |
RefSeqi | NP_011303.1, NM_001181077.1 |
Genome annotation databases
EnsemblFungii | YGL212W_mRNA; YGL212W; YGL212W |
GeneIDi | 852660 |
KEGGi | sce:YGL212W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D11379 Genomic DNA Translation: BAA01977.1 U33754 Genomic DNA Translation: AAC49494.1 Z72734 Genomic DNA Translation: CAA96928.1 BK006941 Genomic DNA Translation: DAA07904.1 |
PIRi | S31263 |
RefSeqi | NP_011303.1, NM_001181077.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KMD | NMR | - | A | 8-124 | [»] | |
AlphaFoldDBi | P32912 | |||||
SMRi | P32912 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33044, 709 interactors |
ComplexPortali | CPX-1887, Vacuolar SNARE complex VAM3-VTI1-VAM7-YKT6 CPX-5401, Vacuolar SNARE complex VAM3-VTI1-VAM7-NYV1 |
DIPi | DIP-1722N |
IntActi | P32912, 36 interactors |
MINTi | P32912 |
STRINGi | 4932.YGL212W |
Protein family/group databases
TCDBi | 1.F.1.1.2, the synaptosomal vesicle fusion pore (svf-pore) family |
PTM databases
iPTMneti | P32912 |
Proteomic databases
MaxQBi | P32912 |
PaxDbi | P32912 |
PRIDEi | P32912 |
Genome annotation databases
EnsemblFungii | YGL212W_mRNA; YGL212W; YGL212W |
GeneIDi | 852660 |
KEGGi | sce:YGL212W |
Organism-specific databases
SGDi | S000003180, VAM7 |
VEuPathDBi | FungiDB:YGL212W |
Phylogenomic databases
eggNOGi | KOG3202, Eukaryota |
HOGENOMi | CLU_033748_1_0_1 |
InParanoidi | P32912 |
OMAi | NITMMRI |
Miscellaneous databases
EvolutionaryTracei | P32912 |
PROi | PR:P32912 |
RNActi | P32912, protein |
Family and domain databases
Gene3Di | 3.30.1520.10, 1 hit |
InterProi | View protein in InterPro IPR001683, PX_dom IPR036871, PX_dom_sf IPR045242, Syntaxin IPR000727, T_SNARE_dom |
PANTHERi | PTHR19957, PTHR19957, 1 hit |
Pfami | View protein in Pfam PF00787, PX, 1 hit |
SMARTi | View protein in SMART SM00312, PX, 1 hit SM00397, t_SNARE, 1 hit |
SUPFAMi | SSF64268, SSF64268, 1 hit |
PROSITEi | View protein in PROSITE PS50195, PX, 1 hit PS50192, T_SNARE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VAM7_YEAST | |
Accessioni | P32912Primary (citable) accession number: P32912 Secondary accession number(s): D6VTU3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | May 25, 2022 | |
This is version 190 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references