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Protein

Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

Gene

MNS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in glycoprotein quality control as it is important for the targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations it further trims the carbohydrates to Man5GlcNAc2.1 Publication

Miscellaneous

Present with 8260 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.1 Publication

Cofactori

Ca2+1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei399Proton donorBy similarity1
Metal bindingi525Calcium1 Publication1

GO - Molecular functioni

GO - Biological processi

  • N-glycan processing Source: GO_Central
  • protein deglycosylation involved in glycoprotein catabolic process Source: SGD
  • protein glycosylation Source: UniProtKB-UniPathway
  • ubiquitin-dependent ERAD pathway Source: SGD

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YJR131W-MONOMER
YEAST:YJR131W-MONOMER
BRENDAi3.2.1.113 984
ReactomeiR-SCE-901032 ER Quality Control Compartment (ERQC)
R-SCE-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC:3.2.1.1131 Publication)
Alternative name(s):
ER alpha-1,2-mannosidase
Man(9)-alpha-mannosidase
Gene namesi
Name:MNS1
Ordered Locus Names:YJR131W
ORF Names:J2110
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

SGDiS000003892 MNS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4CytoplasmicSequence analysis4
Transmembranei5 – 24Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini25 – 354LumenalSequence analysisAdd BLAST330

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103191 – 549Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidaseAdd BLAST549

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi96N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Glycosylationi155N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Glycosylationi224N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Disulfide bondi340 ↔ 385Combined sources1 Publication
Disulfide bondi468 ↔ 471Combined sources1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP32906
PaxDbiP32906
PRIDEiP32906

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi33886, 147 interactors
DIPiDIP-1441N
IntActiP32906, 6 interactors
MINTiP32906
STRINGi4932.YJR131W

Structurei

Secondary structure

1549
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP32906
SMRiP32906
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32906

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016529
HOGENOMiHOG000181987
InParanoidiP32906
KOiK01230
OMAiDHLTCYL
OrthoDBiEOG092C2MQZ

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequencei

Sequence statusi: Complete.

P32906-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKNSVGISIA TIVAIIAAIY YVPWYEHFER KSPGAGEMRD RIESMFLESW
60 70 80 90 100
RDYSKHGWGY DVYGPIEHTS HNMPRGNQPL GWIIVDSVDT LMLMYNSSTL
110 120 130 140 150
YKSEFEAEIQ RSEHWINDVL DFDIDAEVNV FETTIRMLGG LLSAYHLSDV
160 170 180 190 200
LEVGNKTVYL NKAIDLGDRL ALAFLSTQTG IPYSSINLHS GQAVKNHADG
210 220 230 240 250
GASSTAEFTT LQMEFKYLAY LTGNRTYWEL VERVYEPLYK NNDLLNTYDG
260 270 280 290 300
LVPIYTFPDT GKFGASTIRF GSRGDSFYEY LLKQYLLTHE TLYYDLYRKS
310 320 330 340 350
MEGMKKHLLA QSKPSSLWYI GEREQGLHGQ LSPKMDHLVC FMGGLLASGS
360 370 380 390 400
TEGLSIHEAR RRPFFSLSLE RKSDWDLAKG ITDTCYQMYK QSSSGLAPEI
410 420 430 440 450
VVFNDGNIKQ DGWWRSSVGD FFVKPLDRHN LQRPETVESI MFMYHLSHDH
460 470 480 490 500
KYREWGAEIA TSFFENTCVD CNDPKLRRFT SLSDCITLPT KKSNNMESFW
510 520 530 540
LAETLKYLYI LFLDEFDLTK VVFNTEAHPF PVLDEEILKS QSLTTGWSL
Length:549
Mass (Da):63,057
Last modified:October 1, 1993 - v1
Checksum:iB85FF351900A9BB2
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti40D → Y. 1
Natural varianti376D → L. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63598 Genomic DNA Translation: AAA34799.1
Z49631 Genomic DNA Translation: CAA89662.1
BK006943 Genomic DNA Translation: DAA08915.1
PIRiA39345
RefSeqiNP_012665.3, NM_001181789.3

Genome annotation databases

EnsemblFungiiYJR131W; YJR131W; YJR131W
GeneIDi853595
KEGGisce:YJR131W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63598 Genomic DNA Translation: AAA34799.1
Z49631 Genomic DNA Translation: CAA89662.1
BK006943 Genomic DNA Translation: DAA08915.1
PIRiA39345
RefSeqiNP_012665.3, NM_001181789.3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DL2X-ray1.54A34-549[»]
1G6IX-ray1.59A1-549[»]
ProteinModelPortaliP32906
SMRiP32906
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33886, 147 interactors
DIPiDIP-1441N
IntActiP32906, 6 interactors
MINTiP32906
STRINGi4932.YJR131W

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Proteomic databases

MaxQBiP32906
PaxDbiP32906
PRIDEiP32906

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR131W; YJR131W; YJR131W
GeneIDi853595
KEGGisce:YJR131W

Organism-specific databases

SGDiS000003892 MNS1

Phylogenomic databases

GeneTreeiENSGT00390000016529
HOGENOMiHOG000181987
InParanoidiP32906
KOiK01230
OMAiDHLTCYL
OrthoDBiEOG092C2MQZ

Enzyme and pathway databases

UniPathwayi
UPA00378

BioCyciMetaCyc:YJR131W-MONOMER
YEAST:YJR131W-MONOMER
BRENDAi3.2.1.113 984
ReactomeiR-SCE-901032 ER Quality Control Compartment (ERQC)
R-SCE-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2

Miscellaneous databases

EvolutionaryTraceiP32906
PROiPR:P32906

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMNS1_YEAST
AccessioniPrimary (citable) accession number: P32906
Secondary accession number(s): D6VWU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 7, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names
  6. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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