UniProtKB - P32906 (MNS1_YEAST)
Protein
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
Gene
MNS1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in glycoprotein quality control as it is important for the targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations it further trims the carbohydrates to Man5GlcNAc2.1 Publication
Miscellaneous
Present with 8260 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)1 PublicationEC:3.2.1.1131 Publication
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)1 PublicationEC:3.2.1.1131 Publication
Cofactori
Ca2+1 Publication
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 399 | Proton donorBy similarity | 1 | |
Metal bindingi | 525 | Calcium1 Publication | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- N-glycan processing Source: GO_Central
- protein deglycosylation involved in glycoprotein catabolic process Source: SGD
- protein glycosylation Source: UniProtKB-UniPathway
- ubiquitin-dependent ERAD pathway Source: SGD
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:YJR131W-MONOMER |
BRENDAi | 3.2.1.113, 984 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Names & Taxonomyi
Protein namesi | Recommended name: Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC:3.2.1.1131 Publication)Alternative name(s): ER alpha-1,2-mannosidase Man(9)-alpha-mannosidase |
Gene namesi | Name:MNS1 Ordered Locus Names:YJR131W ORF Names:J2110 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YJR131W |
SGDi | S000003892, MNS1 |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 4 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 5 – 24 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 25 – 354 | LumenalSequence analysisAdd BLAST | 330 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
No visible phenotype.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210319 | 1 – 549 | Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidaseAdd BLAST | 549 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 96 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Glycosylationi | 155 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Glycosylationi | 224 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Disulfide bondi | 340 ↔ 385 | Combined sources1 Publication | ||
Disulfide bondi | 468 ↔ 471 | Combined sources1 Publication |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | P32906 |
PaxDbi | P32906 |
PRIDEi | P32906 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 33886, 148 interactors |
DIPi | DIP-1441N |
IntActi | P32906, 6 interactors |
MINTi | P32906 |
STRINGi | 4932.YJR131W |
Miscellaneous databases
RNActi | P32906, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32906 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32906 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2431, Eukaryota |
GeneTreei | ENSGT00940000155422 |
HOGENOMi | CLU_003818_3_0_1 |
InParanoidi | P32906 |
OMAi | RKHLVTY |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
i Sequence
Sequence statusi: Complete.
P32906-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKNSVGISIA TIVAIIAAIY YVPWYEHFER KSPGAGEMRD RIESMFLESW
60 70 80 90 100
RDYSKHGWGY DVYGPIEHTS HNMPRGNQPL GWIIVDSVDT LMLMYNSSTL
110 120 130 140 150
YKSEFEAEIQ RSEHWINDVL DFDIDAEVNV FETTIRMLGG LLSAYHLSDV
160 170 180 190 200
LEVGNKTVYL NKAIDLGDRL ALAFLSTQTG IPYSSINLHS GQAVKNHADG
210 220 230 240 250
GASSTAEFTT LQMEFKYLAY LTGNRTYWEL VERVYEPLYK NNDLLNTYDG
260 270 280 290 300
LVPIYTFPDT GKFGASTIRF GSRGDSFYEY LLKQYLLTHE TLYYDLYRKS
310 320 330 340 350
MEGMKKHLLA QSKPSSLWYI GEREQGLHGQ LSPKMDHLVC FMGGLLASGS
360 370 380 390 400
TEGLSIHEAR RRPFFSLSLE RKSDWDLAKG ITDTCYQMYK QSSSGLAPEI
410 420 430 440 450
VVFNDGNIKQ DGWWRSSVGD FFVKPLDRHN LQRPETVESI MFMYHLSHDH
460 470 480 490 500
KYREWGAEIA TSFFENTCVD CNDPKLRRFT SLSDCITLPT KKSNNMESFW
510 520 530 540
LAETLKYLYI LFLDEFDLTK VVFNTEAHPF PVLDEEILKS QSLTTGWSL
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 40 | D → Y. | 1 | |
Natural varianti | 376 | D → L. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63598 Genomic DNA Translation: AAA34799.1 Z49631 Genomic DNA Translation: CAA89662.1 BK006943 Genomic DNA Translation: DAA08915.1 |
PIRi | A39345 |
RefSeqi | NP_012665.3, NM_001181789.3 |
Genome annotation databases
EnsemblFungii | YJR131W_mRNA; YJR131W; YJR131W |
GeneIDi | 853595 |
KEGGi | sce:YJR131W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63598 Genomic DNA Translation: AAA34799.1 Z49631 Genomic DNA Translation: CAA89662.1 BK006943 Genomic DNA Translation: DAA08915.1 |
PIRi | A39345 |
RefSeqi | NP_012665.3, NM_001181789.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DL2 | X-ray | 1.54 | A | 34-549 | [»] | |
1G6I | X-ray | 1.59 | A | 1-549 | [»] | |
SMRi | P32906 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33886, 148 interactors |
DIPi | DIP-1441N |
IntActi | P32906, 6 interactors |
MINTi | P32906 |
STRINGi | 4932.YJR131W |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Proteomic databases
MaxQBi | P32906 |
PaxDbi | P32906 |
PRIDEi | P32906 |
Genome annotation databases
EnsemblFungii | YJR131W_mRNA; YJR131W; YJR131W |
GeneIDi | 853595 |
KEGGi | sce:YJR131W |
Organism-specific databases
EuPathDBi | FungiDB:YJR131W |
SGDi | S000003892, MNS1 |
Phylogenomic databases
eggNOGi | KOG2431, Eukaryota |
GeneTreei | ENSGT00940000155422 |
HOGENOMi | CLU_003818_3_0_1 |
InParanoidi | P32906 |
OMAi | RKHLVTY |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BioCyci | MetaCyc:YJR131W-MONOMER |
BRENDAi | 3.2.1.113, 984 |
Miscellaneous databases
EvolutionaryTracei | P32906 |
PROi | PR:P32906 |
RNActi | P32906, protein |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MNS1_YEAST | |
Accessioni | P32906Primary (citable) accession number: P32906 Secondary accession number(s): D6VWU9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 189 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries