UniProtKB - P32883 (RASK_MOUSE)
GTPase KRas
Kras
Functioni
Miscellaneous
Catalytic activityi
- EC:3.6.5.2By similarity
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 18 | GTPBy similarity | 9 | |
Nucleotide bindingi | 29 – 35 | GTPBy similarity | 7 | |
Nucleotide bindingi | 59 – 60 | GTPBy similarity | 2 | |
Nucleotide bindingi | 116 – 119 | GTPBy similarity | 4 |
GO - Molecular functioni
- GDP binding Source: MGI
- GMP binding Source: MGI
- GTPase activity Source: MGI
- GTP binding Source: GO_Central
- identical protein binding Source: MGI
- LRR domain binding Source: MGI
- protein-containing complex binding Source: MGI
GO - Biological processi
- actin cytoskeleton organization Source: MGI
- cytokine-mediated signaling pathway Source: MGI
- endocrine signaling Source: CACAO
- epithelial tube branching involved in lung morphogenesis Source: MGI
- female pregnancy Source: Ensembl
- forebrain astrocyte development Source: MGI
- homeostasis of number of cells within a tissue Source: MGI
- liver development Source: Ensembl
- negative regulation of cell differentiation Source: MGI
- negative regulation of neuron apoptotic process Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of cellular senescence Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of MAP kinase activity Source: MGI
- positive regulation of NF-kappaB transcription factor activity Source: MGI
- positive regulation of nitric-oxide synthase activity Source: MGI
- positive regulation of protein phosphorylation Source: MGI
- positive regulation of Rac protein signal transduction Source: MGI
- Ras protein signal transduction Source: MGI
- regulation of long-term neuronal synaptic plasticity Source: MGI
- regulation of protein stability Source: UniProtKB
- regulation of synaptic transmission, GABAergic Source: MGI
- response to glucocorticoid Source: Ensembl
- response to isolation stress Source: Ensembl
- response to mineralocorticoid Source: Ensembl
- striated muscle cell differentiation Source: MGI
- visual learning Source: MGI
Keywordsi
Molecular function | Hydrolase |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1169092, Activation of RAS in B cells R-MMU-1250347, SHC1 events in ERBB4 signaling R-MMU-1433557, Signaling by SCF-KIT R-MMU-171007, p38MAPK events R-MMU-179812, GRB2 events in EGFR signaling R-MMU-180336, SHC1 events in EGFR signaling R-MMU-186763, Downstream signal transduction R-MMU-1963640, GRB2 events in ERBB2 signaling R-MMU-210993, Tie2 Signaling R-MMU-2179392, EGFR Transactivation by Gastrin R-MMU-2424491, DAP12 signaling R-MMU-2871796, FCERI mediated MAPK activation R-MMU-375165, NCAM signaling for neurite out-growth R-MMU-4086398, Ca2+ pathway R-MMU-5218921, VEGFR2 mediated cell proliferation R-MMU-5621575, CD209 (DC-SIGN) signaling R-MMU-5654688, SHC-mediated cascade:FGFR1 R-MMU-5654693, FRS-mediated FGFR1 signaling R-MMU-5654699, SHC-mediated cascade:FGFR2 R-MMU-5654700, FRS-mediated FGFR2 signaling R-MMU-5654704, SHC-mediated cascade:FGFR3 R-MMU-5654706, FRS-mediated FGFR3 signaling R-MMU-5654712, FRS-mediated FGFR4 signaling R-MMU-5654719, SHC-mediated cascade:FGFR4 R-MMU-5658442, Regulation of RAS by GAPs R-MMU-5673000, RAF activation R-MMU-5673001, RAF/MAP kinase cascade R-MMU-5674135, MAP2K and MAPK activation R-MMU-5675221, Negative regulation of MAPK pathway R-MMU-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases R-MMU-8851805, MET activates RAS signaling R-MMU-8951936, RUNX3 regulates p14-ARF R-MMU-9607240, FLT3 Signaling R-MMU-9634635, Estrogen-stimulated signaling through PRKCZ R-MMU-9648002, RAS processing |
Names & Taxonomyi
Protein namesi | Recommended name: GTPase KRas (EC:3.6.5.2By similarity)Alternative name(s): K-Ras 2 Ki-Ras c-K-ras c-Ki-ras Cleaved into the following chain: |
Gene namesi | Name:Kras Synonyms:Kras2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96680, Kras |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Other locations
- Cytoplasm By similarity
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity
Mitochondrion
- mitochondrion Source: MGI
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- membrane Source: MGI
- membrane raft Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000326482 | 1 – 186 | GTPase KRasAdd BLAST | 186 | |
Initiator methioninei | Removed; alternateBy similarity | |||
ChainiPRO_0000082643 | 2 – 186 | GTPase KRas, N-terminally processedAdd BLAST | 185 | |
PropeptideiPRO_0000281293 | 187 – 189 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 2 | N-acetylthreonine; in GTPase KRas, N-terminally processedBy similarity | 1 | |
Modified residuei | 104 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 170 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Lipidationi | 180 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 186 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 186 | S-farnesyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Lipoprotein, Methylation, Palmitate, Prenylation, Ubl conjugationProteomic databases
jPOSTi | P32883 |
PaxDbi | P32883 |
PeptideAtlasi | P32883 |
PRIDEi | P32883 |
PTM databases
iPTMneti | P32883 |
PhosphoSitePlusi | P32883 |
SwissPalmi | P32883 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000030265, Expressed in ureteric bud tip and 304 other tissues |
ExpressionAtlasi | P32883, baseline and differential |
Genevisiblei | P32883, MM |
Interactioni
Subunit structurei
Interacts with PHLPP.
Interacts (active GTP-bound form preferentially) with RGS14 (By similarity).
Interacts (when farnesylated) with PDE6D; this promotes dissociation from the cell membrane.
Interacts with SOS1 (By similarity).
Interacts (when farnesylated) with GPR31 (By similarity).
By similarityBinary interactionsi
Hide detailsP32883
With | #Exp. | IntAct |
---|---|---|
Egfr [Q01279] | 3 | EBI-644267,EBI-6296235 |
RAF1 [P04049] from Homo sapiens. | 2 | EBI-644267,EBI-365996 |
Isoform 2B [P32883-2]
With | #Exp. | IntAct |
---|---|---|
Raf1 [Q99N57] | 3 | EBI-644285,EBI-397757 |
GO - Molecular functioni
- identical protein binding Source: MGI
- LRR domain binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201012, 5 interactors |
DIPi | DIP-29362N |
IntActi | P32883, 8 interactors |
MINTi | P32883 |
STRINGi | 10090.ENSMUSP00000032399 |
Miscellaneous databases
RNActi | P32883, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 166 – 185 | Hypervariable regionAdd BLAST | 20 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 32 – 40 | Effector region | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000155871 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P32883 |
OMAi | EYKLVVM |
OrthoDBi | 1259506at2759 |
PhylomeDBi | P32883 |
TreeFami | TF312796 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI
110 120 130 140 150
KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QELARSYGIP FIETSAKTRQ
160 170 180
RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKCVIM
The sequence of this isoform differs from the canonical sequence as follows:
151-153: RVE → GVD
165-189: QYRLKKISKEEKTPGCVKIKKCVIM → KHKEKMSKDGKKKKKKSRTRCTVM
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0N4SVY1 | A0A0N4SVY1_MOUSE | GTPase KRas | Kras | 75 | Annotation score: | ||
B2KGV5 | B2KGV5_MOUSE | GTPase KRas | Kras | 34 | Annotation score: | ||
E9Q8V2 | E9Q8V2_MOUSE | GTPase KRas | Kras | 40 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011142 | 151 – 153 | RVE → GVD in isoform 2B. 2 Publications | 3 | |
Alternative sequenceiVSP_011143 | 165 – 189 | QYRLK…KCVIM → KHKEKMSKDGKKKKKKSRTR CTVM in isoform 2B. 2 PublicationsAdd BLAST | 25 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02452 , X02453, X02454, X02456 Genomic DNA Translation: CAA26295.1 X02455 Genomic DNA Translation: CAA26296.1 BC004642 mRNA Translation: AAH04642.1 BC010202 mRNA Translation: AAH10202.1 K01927 Genomic DNA Translation: AAA40037.1 X00485 mRNA Translation: CAA25160.1 |
CCDSi | CCDS20693.1 [P32883-2] |
PIRi | A01365, TVMSK B01365, TVMS2K |
RefSeqi | NP_067259.4, NM_021284.6 [P32883-2] XP_006506981.1, XM_006506918.3 [P32883-1] |
Genome annotation databases
Ensembli | ENSMUST00000032399; ENSMUSP00000032399; ENSMUSG00000030265 [P32883-2] ENSMUST00000111710; ENSMUSP00000107339; ENSMUSG00000030265 [P32883-1] |
GeneIDi | 16653 |
KEGGi | mmu:16653 |
UCSCi | uc009eri.2, mouse [P32883-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02452 , X02453, X02454, X02456 Genomic DNA Translation: CAA26295.1 X02455 Genomic DNA Translation: CAA26296.1 BC004642 mRNA Translation: AAH04642.1 BC010202 mRNA Translation: AAH10202.1 K01927 Genomic DNA Translation: AAA40037.1 X00485 mRNA Translation: CAA25160.1 |
CCDSi | CCDS20693.1 [P32883-2] |
PIRi | A01365, TVMSK B01365, TVMS2K |
RefSeqi | NP_067259.4, NM_021284.6 [P32883-2] XP_006506981.1, XM_006506918.3 [P32883-1] |
3D structure databases
BMRBi | P32883 |
SMRi | P32883 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201012, 5 interactors |
DIPi | DIP-29362N |
IntActi | P32883, 8 interactors |
MINTi | P32883 |
STRINGi | 10090.ENSMUSP00000032399 |
PTM databases
iPTMneti | P32883 |
PhosphoSitePlusi | P32883 |
SwissPalmi | P32883 |
Proteomic databases
jPOSTi | P32883 |
PaxDbi | P32883 |
PeptideAtlasi | P32883 |
PRIDEi | P32883 |
Protocols and materials databases
Antibodypediai | 24248, 843 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000032399; ENSMUSP00000032399; ENSMUSG00000030265 [P32883-2] ENSMUST00000111710; ENSMUSP00000107339; ENSMUSG00000030265 [P32883-1] |
GeneIDi | 16653 |
KEGGi | mmu:16653 |
UCSCi | uc009eri.2, mouse [P32883-1] |
Organism-specific databases
CTDi | 3845 |
MGIi | MGI:96680, Kras |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000155871 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P32883 |
OMAi | EYKLVVM |
OrthoDBi | 1259506at2759 |
PhylomeDBi | P32883 |
TreeFami | TF312796 |
Enzyme and pathway databases
Reactomei | R-MMU-1169092, Activation of RAS in B cells R-MMU-1250347, SHC1 events in ERBB4 signaling R-MMU-1433557, Signaling by SCF-KIT R-MMU-171007, p38MAPK events R-MMU-179812, GRB2 events in EGFR signaling R-MMU-180336, SHC1 events in EGFR signaling R-MMU-186763, Downstream signal transduction R-MMU-1963640, GRB2 events in ERBB2 signaling R-MMU-210993, Tie2 Signaling R-MMU-2179392, EGFR Transactivation by Gastrin R-MMU-2424491, DAP12 signaling R-MMU-2871796, FCERI mediated MAPK activation R-MMU-375165, NCAM signaling for neurite out-growth R-MMU-4086398, Ca2+ pathway R-MMU-5218921, VEGFR2 mediated cell proliferation R-MMU-5621575, CD209 (DC-SIGN) signaling R-MMU-5654688, SHC-mediated cascade:FGFR1 R-MMU-5654693, FRS-mediated FGFR1 signaling R-MMU-5654699, SHC-mediated cascade:FGFR2 R-MMU-5654700, FRS-mediated FGFR2 signaling R-MMU-5654704, SHC-mediated cascade:FGFR3 R-MMU-5654706, FRS-mediated FGFR3 signaling R-MMU-5654712, FRS-mediated FGFR4 signaling R-MMU-5654719, SHC-mediated cascade:FGFR4 R-MMU-5658442, Regulation of RAS by GAPs R-MMU-5673000, RAF activation R-MMU-5673001, RAF/MAP kinase cascade R-MMU-5674135, MAP2K and MAPK activation R-MMU-5675221, Negative regulation of MAPK pathway R-MMU-8849471, PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases R-MMU-8851805, MET activates RAS signaling R-MMU-8951936, RUNX3 regulates p14-ARF R-MMU-9607240, FLT3 Signaling R-MMU-9634635, Estrogen-stimulated signaling through PRKCZ R-MMU-9648002, RAS processing |
Miscellaneous databases
BioGRID-ORCSi | 16653, 6 hits in 22 CRISPR screens |
ChiTaRSi | Kras, mouse |
PROi | PR:P32883 |
RNActi | P32883, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030265, Expressed in ureteric bud tip and 304 other tissues |
ExpressionAtlasi | P32883, baseline and differential |
Genevisiblei | P32883, MM |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RASK_MOUSE | |
Accessioni | P32883Primary (citable) accession number: P32883 Secondary accession number(s): P04200, P08643 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | December 2, 2020 | |
This is version 198 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families