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Protein

GTPase KRas

Gene

Kras

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (By similarity). Plays an important role in the regulation of cell proliferation (PubMed:6474169, PubMed:1352876). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (By similarity).By similarity2 Publications

Miscellaneous

This gene is amplified in the mouse adrenal tumor Y1 cells, and is also directly linked to lung tumor susceptibility.

Activity regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Interaction with SOS1 promotes exchange of bound GDP by GTP.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18GTPBy similarity9
Nucleotide bindingi29 – 35GTPBy similarity7
Nucleotide bindingi59 – 60GTPBy similarity2
Nucleotide bindingi116 – 119GTPBy similarity4

GO - Molecular functioni

  • GDP binding Source: MGI
  • GMP binding Source: MGI
  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB-KW
  • identical protein binding Source: MGI
  • LRR domain binding Source: MGI
  • protein-containing complex binding Source: MGI

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1169092 Activation of RAS in B cells
R-MMU-1250347 SHC1 events in ERBB4 signaling
R-MMU-1433557 Signaling by SCF-KIT
R-MMU-171007 p38MAPK events
R-MMU-179812 GRB2 events in EGFR signaling
R-MMU-180336 SHC1 events in EGFR signaling
R-MMU-186763 Downstream signal transduction
R-MMU-1963640 GRB2 events in ERBB2 signaling
R-MMU-210993 Tie2 Signaling
R-MMU-2179392 EGFR Transactivation by Gastrin
R-MMU-2424491 DAP12 signaling
R-MMU-2871796 FCERI mediated MAPK activation
R-MMU-375165 NCAM signaling for neurite out-growth
R-MMU-5218921 VEGFR2 mediated cell proliferation
R-MMU-5621575 CD209 (DC-SIGN) signaling
R-MMU-5654688 SHC-mediated cascade:FGFR1
R-MMU-5654693 FRS-mediated FGFR1 signaling
R-MMU-5654699 SHC-mediated cascade:FGFR2
R-MMU-5654700 FRS-mediated FGFR2 signaling
R-MMU-5654704 SHC-mediated cascade:FGFR3
R-MMU-5654706 FRS-mediated FGFR3 signaling
R-MMU-5654712 FRS-mediated FGFR4 signaling
R-MMU-5654719 SHC-mediated cascade:FGFR4
R-MMU-5658442 Regulation of RAS by GAPs
R-MMU-5673000 RAF activation
R-MMU-5673001 RAF/MAP kinase cascade
R-MMU-5674135 MAP2K and MAPK activation
R-MMU-5675221 Negative regulation of MAPK pathway
R-MMU-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-MMU-8851805 MET activates RAS signaling
R-MMU-8951936 RUNX3 regulates p14-ARF

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase KRas
Alternative name(s):
K-Ras 2
Ki-Ras
c-K-ras
c-Ki-ras
Cleaved into the following chain:
Gene namesi
Name:Kras
Synonyms:Kras2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:96680 Kras

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12G → D: Activates oncogene. 1
Mutagenesisi164R → A: Loss of GTP-binding activity. 1

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003264821 – 186GTPase KRasAdd BLAST186
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00000826432 – 186GTPase KRas, N-terminally processedAdd BLAST185
PropeptideiPRO_0000281293187 – 189Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in GTPase KRas, N-terminally processedBy similarity1
Modified residuei104N6-acetyllysineBy similarity1
Lipidationi180S-palmitoyl cysteineBy similarity1
Modified residuei186Cysteine methyl esterBy similarity1
Lipidationi186S-farnesyl cysteineBy similarity1

Post-translational modificationi

Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

PaxDbiP32883
PeptideAtlasiP32883
PRIDEiP32883

PTM databases

iPTMnetiP32883
PhosphoSitePlusiP32883
SwissPalmiP32883

Expressioni

Gene expression databases

BgeeiENSMUSG00000030265 Expressed in 291 organ(s), highest expression level in ureteric bud tip
ExpressionAtlasiP32883 baseline and differential
GenevisibleiP32883 MM

Interactioni

Subunit structurei

Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14 (By similarity). Interacts (when farnesylated) with PDE6D; this promotes dissociation from the cell membrane (By similarity). Interacts with SOS1 (By similarity).By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201012, 4 interactors
DIPiDIP-29362N
IntActiP32883, 8 interactors
MINTiP32883
STRINGi10090.ENSMUSP00000032399

Structurei

3D structure databases

ProteinModelPortaliP32883
SMRiP32883
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 185Hypervariable regionAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector region9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

GeneTreeiENSGT00860000133672
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiP32883
KOiK07827
OMAiMSKEGKK
OrthoDBiEOG091G0UAU
PhylomeDBiP32883
TreeFamiTF312796

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Isoforms differ in the C-terminal region which is encoded by two alternative exons (IVA and IVB).

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 2A (identifier: P32883-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI
110 120 130 140 150
KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QELARSYGIP FIETSAKTRQ
160 170 180
RVEDAFYTLV REIRQYRLKK ISKEEKTPGC VKIKKCVIM
Length:189
Mass (Da):21,656
Last modified:August 13, 1987 - v1
Checksum:i97345422E01D2C81
GO
Isoform 2B (identifier: P32883-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     151-153: RVE → GVD
     165-189: QYRLKKISKEEKTPGCVKIKKCVIM → KHKEKMSKDGKKKKKKSRTRCTVM

Show »
Length:188
Mass (Da):21,483
Checksum:iB0A542E8AD158F51
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B2KGV5B2KGV5_MOUSE
GTPase KRas
Kras
34Annotation score:
E9Q8V2E9Q8V2_MOUSE
GTPase KRas
Kras
40Annotation score:
A0A0N4SVY1A0A0N4SVY1_MOUSE
GTPase KRas
Kras
75Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011142151 – 153RVE → GVD in isoform 2B. 2 Publications3
Alternative sequenceiVSP_011143165 – 189QYRLK…KCVIM → KHKEKMSKDGKKKKKKSRTR CTVM in isoform 2B. 2 PublicationsAdd BLAST25

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02452
, X02453, X02454, X02456 Genomic DNA Translation: CAA26295.1
X02455 Genomic DNA Translation: CAA26296.1
BC004642 mRNA Translation: AAH04642.1
BC010202 mRNA Translation: AAH10202.1
K01927 Genomic DNA Translation: AAA40037.1
X00485 mRNA Translation: CAA25160.1
CCDSiCCDS20693.1 [P32883-2]
PIRiA01365 TVMSK
B01365 TVMS2K
RefSeqiNP_067259.4, NM_021284.6 [P32883-2]
XP_006506981.1, XM_006506918.3 [P32883-1]
UniGeneiMm.383182

Genome annotation databases

EnsembliENSMUST00000032399; ENSMUSP00000032399; ENSMUSG00000030265 [P32883-2]
ENSMUST00000111710; ENSMUSP00000107339; ENSMUSG00000030265 [P32883-1]
GeneIDi16653
KEGGimmu:16653
UCSCiuc009eri.2 mouse [P32883-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02452
, X02453, X02454, X02456 Genomic DNA Translation: CAA26295.1
X02455 Genomic DNA Translation: CAA26296.1
BC004642 mRNA Translation: AAH04642.1
BC010202 mRNA Translation: AAH10202.1
K01927 Genomic DNA Translation: AAA40037.1
X00485 mRNA Translation: CAA25160.1
CCDSiCCDS20693.1 [P32883-2]
PIRiA01365 TVMSK
B01365 TVMS2K
RefSeqiNP_067259.4, NM_021284.6 [P32883-2]
XP_006506981.1, XM_006506918.3 [P32883-1]
UniGeneiMm.383182

3D structure databases

ProteinModelPortaliP32883
SMRiP32883
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201012, 4 interactors
DIPiDIP-29362N
IntActiP32883, 8 interactors
MINTiP32883
STRINGi10090.ENSMUSP00000032399

PTM databases

iPTMnetiP32883
PhosphoSitePlusiP32883
SwissPalmiP32883

Proteomic databases

PaxDbiP32883
PeptideAtlasiP32883
PRIDEiP32883

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032399; ENSMUSP00000032399; ENSMUSG00000030265 [P32883-2]
ENSMUST00000111710; ENSMUSP00000107339; ENSMUSG00000030265 [P32883-1]
GeneIDi16653
KEGGimmu:16653
UCSCiuc009eri.2 mouse [P32883-1]

Organism-specific databases

CTDi3845
MGIiMGI:96680 Kras

Phylogenomic databases

GeneTreeiENSGT00860000133672
HOGENOMiHOG000233973
HOVERGENiHBG009351
InParanoidiP32883
KOiK07827
OMAiMSKEGKK
OrthoDBiEOG091G0UAU
PhylomeDBiP32883
TreeFamiTF312796

Enzyme and pathway databases

ReactomeiR-MMU-1169092 Activation of RAS in B cells
R-MMU-1250347 SHC1 events in ERBB4 signaling
R-MMU-1433557 Signaling by SCF-KIT
R-MMU-171007 p38MAPK events
R-MMU-179812 GRB2 events in EGFR signaling
R-MMU-180336 SHC1 events in EGFR signaling
R-MMU-186763 Downstream signal transduction
R-MMU-1963640 GRB2 events in ERBB2 signaling
R-MMU-210993 Tie2 Signaling
R-MMU-2179392 EGFR Transactivation by Gastrin
R-MMU-2424491 DAP12 signaling
R-MMU-2871796 FCERI mediated MAPK activation
R-MMU-375165 NCAM signaling for neurite out-growth
R-MMU-5218921 VEGFR2 mediated cell proliferation
R-MMU-5621575 CD209 (DC-SIGN) signaling
R-MMU-5654688 SHC-mediated cascade:FGFR1
R-MMU-5654693 FRS-mediated FGFR1 signaling
R-MMU-5654699 SHC-mediated cascade:FGFR2
R-MMU-5654700 FRS-mediated FGFR2 signaling
R-MMU-5654704 SHC-mediated cascade:FGFR3
R-MMU-5654706 FRS-mediated FGFR3 signaling
R-MMU-5654712 FRS-mediated FGFR4 signaling
R-MMU-5654719 SHC-mediated cascade:FGFR4
R-MMU-5658442 Regulation of RAS by GAPs
R-MMU-5673000 RAF activation
R-MMU-5673001 RAF/MAP kinase cascade
R-MMU-5674135 MAP2K and MAPK activation
R-MMU-5675221 Negative regulation of MAPK pathway
R-MMU-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-MMU-8851805 MET activates RAS signaling
R-MMU-8951936 RUNX3 regulates p14-ARF

Miscellaneous databases

ChiTaRSiKras mouse
PROiPR:P32883
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030265 Expressed in 291 organ(s), highest expression level in ureteric bud tip
ExpressionAtlasiP32883 baseline and differential
GenevisibleiP32883 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRASK_MOUSE
AccessioniPrimary (citable) accession number: P32883
Secondary accession number(s): P04200, P08643
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: August 13, 1987
Last modified: November 7, 2018
This is version 183 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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