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Protein

Syntaxin-1A

Gene

Stx1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in hormone and neurotransmitter exocytosis (PubMed:17301173, PubMed:18167541, PubMed:20484665, PubMed:22411134, PubMed:7901002). Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm.1 Publication4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei253 – 254(Microbial infection) Cleavage; by C.botulinum neurotoxin type C (BoNT/C)1 Publication2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processExocytosis, Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-181429 Serotonin Neurotransmitter Release Cycle
R-RNO-181430 Norepinephrine Neurotransmitter Release Cycle
R-RNO-210500 Glutamate Neurotransmitter Release Cycle
R-RNO-212676 Dopamine Neurotransmitter Release Cycle
R-RNO-264642 Acetylcholine Neurotransmitter Release Cycle
R-RNO-449836 Other interleukin signaling
R-RNO-5682910 LGI-ADAM interactions
R-RNO-888590 GABA synthesis, release, reuptake and degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-1A
Alternative name(s):
Neuron-specific antigen HPC-1
Synaptotagmin-associated 35 kDa protein
Short name:
P35A
Gene namesi
Name:Stx1a
Synonyms:Sap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi69430 Stx1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 265CytoplasmicSequence analysisAdd BLAST265
Transmembranei266 – 288Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST23

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101891 – 288Syntaxin-1AAdd BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineCombined sources1
Modified residuei64PhosphoserineCombined sources1
Modified residuei95PhosphoserineCombined sources1
Modified residuei188Phosphoserine; by DAPK11 Publication1

Post-translational modificationi

Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1.1 Publication
(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type C (BoNT/C), which hydrolyzes the 253-Lys-|-Ala-254 bond (PubMed:7737992). Cleavage inhibits neurotransmitter release (PubMed:7901002).2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP32851
PRIDEiP32851

PTM databases

iPTMnetiP32851
PhosphoSitePlusiP32851
SwissPalmiP32851

Miscellaneous databases

PMAP-CutDBiP32851

Expressioni

Tissue specificityi

Expressed predominantly in cerebral cortex, hippocampus, cerebellum, adrenal medulla and retina with weak expression detected in non-neuronal tissues.

Gene expression databases

BgeeiENSRNOG00000029165 Expressed in 10 organ(s), highest expression level in brain
ExpressionAtlasiP32851 baseline and differential
GenevisibleiP32851 RN

Interactioni

Subunit structurei

Interacts (via C-terminus) with KCNB1 (via C-terminus); the interaction increases in a calcium-dependent manner and induces a pore-independent enhancement of exocytosis in neuroendocrine cells, chromaffin cells, pancreatic beta cells and from the soma of dorsal root ganglia (DRG) neurons (PubMed:17301173, PubMed:18167541, PubMed:20484665, PubMed:22411134). Interacts with OTOF, SYTL4, VAPA, SYBU and LGI3 (By similarity). Found in a complex with VAMP8 and SNAP23 (PubMed:9507000, PubMed:10336434). Interacts with SYT6 and SYT8; the interaction is Ca2+-dependent (By similarity). Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A (PubMed:11533035, PubMed:11832227, PubMed:12496247, PubMed:9759724). This complex binds to CPLX1 (PubMed:11832227). Binds STXBP6 (PubMed:12145319). Found in a ternary complex with STX1A and SNAP25 (PubMed:11533035). Interacts with PLCL1 (via C2 domain) (PubMed:23341457). Interacts with SLC6A4 (PubMed:11709063). Interacts with STXBP1 and DAPK1 (PubMed:10746715, PubMed:12730201). Interacts with PRRT2 (By similarity).By similarity15 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi250448, 11 interactors
CORUMiP32851
DIPiDIP-108N
IntActiP32851, 24 interactors
MINTiP32851
STRINGi10116.ENSRNOP00000040699

Structurei

Secondary structure

1288
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00155
ProteinModelPortaliP32851
SMRiP32851
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32851

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini192 – 254t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili68 – 109Sequence analysisAdd BLAST42

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 19Asp-rich (acidic)7

Sequence similaritiesi

Belongs to the syntaxin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0810 Eukaryota
COG5074 LUCA
GeneTreeiENSGT00760000119200
HOGENOMiHOG000286023
HOVERGENiHBG000497
InParanoidiP32851
KOiK04560
OMAiFEEIDHI
OrthoDBiEOG091G0EUK
PhylomeDBiP32851
TreeFamiTF313763

Family and domain databases

CDDicd00179 SynN, 1 hit
InterProiView protein in InterPro
IPR010989 SNARE
IPR028669 STX1A
IPR006012 Syntaxin/epimorphin_CS
IPR006011 Syntaxin_N
IPR000727 T_SNARE_dom
PANTHERiPTHR19957:SF84 PTHR19957:SF84, 1 hit
PfamiView protein in Pfam
PF05739 SNARE, 1 hit
PF00804 Syntaxin, 1 hit
SMARTiView protein in SMART
SM00503 SynN, 1 hit
SM00397 t_SNARE, 1 hit
SUPFAMiSSF47661 SSF47661, 1 hit
PROSITEiView protein in PROSITE
PS00914 SYNTAXIN, 1 hit
PS50192 T_SNARE, 1 hit

Sequencei

Sequence statusi: Complete.

P32851-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKDRTQELRT AKDSDDDDDV TVTVDRDRFM DEFFEQVEEI RGFIDKIAEN
60 70 80 90 100
VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI
110 120 130 140 150
EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ
160 170 180 190 200
RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS
210 220 230 240 250
EIIKLENSIR ELHDMFMDMA MLVESQGEMI DRIEYNVEHA VDYVERAVSD
260 270 280
TKKAVKYQSK ARRKKIMIII CCVILGIIIA STIGGIFG
Length:288
Mass (Da):33,067
Last modified:October 1, 1993 - v1
Checksum:i8F929F489E323CAA
GO

Sequence cautioni

The sequence BAA01231 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10392 mRNA Translation: BAA01231.1 Different initiation.
D12519 mRNA Translation: BAA02089.1
M95734 mRNA Translation: AAA42195.1
PIRiA38141
RefSeqiNP_446240.2, NM_053788.2
UniGeneiRn.9943

Genome annotation databases

EnsembliENSRNOT00000049601; ENSRNOP00000040699; ENSRNOG00000029165
GeneIDi116470
KEGGirno:116470
UCSCiRGD:69430 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10392 mRNA Translation: BAA01231.1 Different initiation.
D12519 mRNA Translation: BAA02089.1
M95734 mRNA Translation: AAA42195.1
PIRiA38141
RefSeqiNP_446240.2, NM_053788.2
UniGeneiRn.9943

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BR0NMR-A27-144[»]
1EZ3X-ray1.90A/B/C24-150[»]
1HVVX-ray2.40A/B/C/D190-264[»]
1JTHX-ray2.00B/D191-267[»]
1KILX-ray2.30B191-250[»]
1N7SX-ray1.45B191-256[»]
1SFCX-ray2.40B/F/J180-262[»]
1URQX-ray2.00B188-259[»]
2BU0model-B191-258[»]
2M8RNMR-A183-288[»]
2N1TNMR-B188-259[»]
3C98X-ray2.60B1-267[»]
3HD7X-ray3.40B/F183-288[»]
3IPDX-ray4.80B/F183-288[»]
3J96electron microscopy7.60L191-256[»]
3J97electron microscopy7.80L191-256[»]
3J98electron microscopy8.40L191-256[»]
3J99electron microscopy8.20L191-256[»]
3RK2X-ray2.20B/F191-253[»]
3RK3X-ray3.50B191-253[»]
3RL0X-ray3.80B/F/J/N/R/V/Z/d191-253[»]
4JEHX-ray2.50B25-266[»]
4JEUX-ray3.20B2-243[»]
5CCGX-ray3.50B/H191-256[»]
5CCHX-ray3.60B191-256[»]
5CCIX-ray4.10B191-256[»]
5KJ7X-ray3.50B/H191-256[»]
5KJ8X-ray4.10B/H191-256[»]
5W5CX-ray1.85B190-256[»]
5W5DX-ray2.50B190-256[»]
6MDMelectron microscopy4.40I1-256[»]
6MDNelectron microscopy4.40I1-256[»]
DisProtiDP00155
ProteinModelPortaliP32851
SMRiP32851
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250448, 11 interactors
CORUMiP32851
DIPiDIP-108N
IntActiP32851, 24 interactors
MINTiP32851
STRINGi10116.ENSRNOP00000040699

PTM databases

iPTMnetiP32851
PhosphoSitePlusiP32851
SwissPalmiP32851

Proteomic databases

PaxDbiP32851
PRIDEiP32851

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000049601; ENSRNOP00000040699; ENSRNOG00000029165
GeneIDi116470
KEGGirno:116470
UCSCiRGD:69430 rat

Organism-specific databases

CTDi6804
RGDi69430 Stx1a

Phylogenomic databases

eggNOGiKOG0810 Eukaryota
COG5074 LUCA
GeneTreeiENSGT00760000119200
HOGENOMiHOG000286023
HOVERGENiHBG000497
InParanoidiP32851
KOiK04560
OMAiFEEIDHI
OrthoDBiEOG091G0EUK
PhylomeDBiP32851
TreeFamiTF313763

Enzyme and pathway databases

ReactomeiR-RNO-181429 Serotonin Neurotransmitter Release Cycle
R-RNO-181430 Norepinephrine Neurotransmitter Release Cycle
R-RNO-210500 Glutamate Neurotransmitter Release Cycle
R-RNO-212676 Dopamine Neurotransmitter Release Cycle
R-RNO-264642 Acetylcholine Neurotransmitter Release Cycle
R-RNO-449836 Other interleukin signaling
R-RNO-5682910 LGI-ADAM interactions
R-RNO-888590 GABA synthesis, release, reuptake and degradation

Miscellaneous databases

EvolutionaryTraceiP32851
PMAP-CutDBiP32851
PROiPR:P32851

Gene expression databases

BgeeiENSRNOG00000029165 Expressed in 10 organ(s), highest expression level in brain
ExpressionAtlasiP32851 baseline and differential
GenevisibleiP32851 RN

Family and domain databases

CDDicd00179 SynN, 1 hit
InterProiView protein in InterPro
IPR010989 SNARE
IPR028669 STX1A
IPR006012 Syntaxin/epimorphin_CS
IPR006011 Syntaxin_N
IPR000727 T_SNARE_dom
PANTHERiPTHR19957:SF84 PTHR19957:SF84, 1 hit
PfamiView protein in Pfam
PF05739 SNARE, 1 hit
PF00804 Syntaxin, 1 hit
SMARTiView protein in SMART
SM00503 SynN, 1 hit
SM00397 t_SNARE, 1 hit
SUPFAMiSSF47661 SSF47661, 1 hit
PROSITEiView protein in PROSITE
PS00914 SYNTAXIN, 1 hit
PS50192 T_SNARE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTX1A_RAT
AccessioniPrimary (citable) accession number: P32851
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 7, 2018
This is version 189 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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