UniProtKB - P32849 (RAD5_YEAST)
DNA repair protein RAD5
RAD5
Functioni
Probable helicase, member of the UBC2/RAD6 epistasis group. Functions with the DNA repair protein RAD18 in error-free postreplication DNA repair. Involved in the maintenance of wild-type rates of instability of simple repetitive sequences such as poly(GT) repeats. Seems to be involved in maintaining a balance which acts in favor of error-prone non-homologous joining during DNA double-strand breaks repairs. Recruits the UBC13-MMS2 dimer to chromatin for DNA repair.
8 PublicationsMiscellaneous
Cofactori
Kineticsi
- KM=525 µM for ATP
pH dependencei
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 532 – 539 | ATPPROSITE-ProRule annotation | 8 | |
Zinc fingeri | 914 – 961 | RING-typePROSITE-ProRule annotationAdd BLAST | 48 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP-dependent activity, acting on DNA Source: SGD
- four-way junction DNA binding Source: SGD
- four-way junction helicase activity Source: SGD
- hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides Source: InterPro
- Y-form DNA binding Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- double-strand break repair Source: SGD
- error-free postreplication DNA repair Source: SGD
- error-free translesion synthesis Source: SGD
- error-prone translesion synthesis Source: SGD
- free ubiquitin chain polymerization Source: SGD
- postreplication repair Source: SGD
- protein polyubiquitination Source: SGD
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: DNA repair protein RAD5 (EC:3.6.4.-)Alternative name(s): Radiation sensitivity protein 5 Revertibility protein 2 |
Gene namesi | Name:RAD5 Synonyms:REV2, SNM2 Ordered Locus Names:YLR032W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004022, RAD5 |
VEuPathDBi | FungiDB:YLR032W |
Subcellular locationi
Nucleus
- nucleus Source: SGD
Other locations
- chromatin Source: SGD
- chromosome, telomeric region Source: SGD
- cytoplasm Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 538 – 539 | KT → AA: Increased sensitivity toward ionizing radiation. 1 Publication | 2 | |
Mutagenesisi | 914 | C → S: Abolishes interaction with UBC13. 2 Publications | 1 | |
Mutagenesisi | 916 | I → A: Abolishes interaction with UBC13. 1 Publication | 1 | |
Mutagenesisi | 944 | Y → A: Abolishes interaction with UBC13. 1 Publication | 1 | |
Mutagenesisi | 959 | N → A: Abolishes interaction with UBC13. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000056130 | 2 – 1169 | DNA repair protein RAD5Add BLAST | 1168 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 20 | PhosphoserineCombined sources | 1 | |
Modified residuei | 23 | PhosphoserineCombined sources | 1 | |
Modified residuei | 129 | PhosphoserineCombined sources | 1 | |
Modified residuei | 130 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P32849 |
PaxDbi | P32849 |
PRIDEi | P32849 |
PTM databases
iPTMneti | P32849 |
Interactioni
Subunit structurei
Homodimer.
Interacts with POL30, RAD18, UBC9 and UBC13.
4 PublicationsProtein-protein interaction databases
BioGRIDi | 31306, 356 interactors |
DIPi | DIP-5830N |
IntActi | P32849, 5 interactors |
MINTi | P32849 |
STRINGi | 4932.YLR032W |
Miscellaneous databases
RNActi | P32849, protein |
Structurei
Secondary structure
3D structure databases
SASBDBi | P32849 |
SMRi | P32849 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 519 – 730 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 212 | |
Domaini | 995 – 1165 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 171 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 302 – 327 | DisorderedSequence analysisAdd BLAST | 26 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 681 – 684 | DEGH box | 4 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 914 – 961 | RING-typePROSITE-ProRule annotationAdd BLAST | 48 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG1001, Eukaryota |
GeneTreei | ENSGT00940000165376 |
HOGENOMi | CLU_000315_2_5_1 |
InParanoidi | P32849 |
OMAi | KVVRFIM |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit 3.40.50.10810, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR014905, HIRAN IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF08797, HIRAN, 1 hit PF00176, SNF2-rel_dom, 1 hit PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00910, HIRAN, 1 hit SM00184, RING, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSHIEQEERK RFFNDDLDTS ETSLNFKSEN KESFLFANSH NDDDDDVVVS
60 70 80 90 100
VSDTTEGEGD RSIVPVRREI EEEGQNQFIT ELLRIIPEMP KDLVMELNEK
110 120 130 140 150
FGSQEEGLSL ALSHYFDHNS GTSISKIPSS PNQLNTLSDT SNSTLSPSSF
160 170 180 190 200
HPKRRRIYGF RNQTRLEDKV TWKRFIGALQ VTGMATRPTV RPLKYGSQMK
210 220 230 240 250
LKRSSEEISA TKVYDSRGRK KASMASLVRI FDIQYDREIG RVSEDIAQIL
260 270 280 290 300
YPLLSSHEIS FEVTLIFCDN KRLSIGDSFI LQLDCFLTSL IFEERNDGES
310 320 330 340 350
LMKRRRTEGG NKREKDNGNF GRTLTETDEE LESRSKRLAL LKLFDKLRLK
360 370 380 390 400
PILDEQKALE KHKIELNSDP EIIDLDNDEI CSNQVTEVHN NLRDTQHEEE
410 420 430 440 450
TMNLNQLKTF YKAAQSSESL KSLPETEPSR DVFKLELRNY QKQGLTWMLR
460 470 480 490 500
REQEFAKAAS DGEASETGAN MINPLWKQFK WPNDMSWAAQ NLQQDHVNVE
510 520 530 540 550
DGIFFYANLH SGEFSLAKPI LKTMIKGGIL SDEMGLGKTV AAYSLVLSCP
560 570 580 590 600
HDSDVVDKKL FDIENTAVSD NLPSTWQDNK KPYASKTTLI VVPMSLLTQW
610 620 630 640 650
SNEFTKANNS PDMYHEVYYG GNVSSLKTLL TKTKTPPTVV LTTYGIVQNE
660 670 680 690 700
WTKHSKGRMT DEDVNISSGL FSVNFYRIII DEGHNIRNRT TVTSKAVMAL
710 720 730 740 750
QGKCKWVLTG TPIINRLDDL YSLVKFLELD PWRQINYWKT FVSTPFESKN
760 770 780 790 800
YKQAFDVVNA ILEPVLLRRT KQMKDKDGKP LVELPPKEVV IKRLPFSKSQ
810 820 830 840 850
DLLYKFLLDK AEVSVKSGIA RGDLLKKYST ILVHILRLRQ VCCHPGLIGS
860 870 880 890 900
QDENDEDLSK NNKLVTEQTV ELDSLMRVVS ERFDNSFSKE ELDAMIQRLK
910 920 930 940 950
VKYPDNKSFQ SLECSICTTE PMDLDKALFT ECGHSFCEKC LFEYIEFQNS
960 970 980 990 1000
KNLGLKCPNC RNQIDACRLL ALVQTNSNSK NLEFKPYSPA SKSSKITALL
1010 1020 1030 1040 1050
KELQLLQDSS AGEQVVIFSQ FSTYLDILEK ELTHTFSKDV AKIYKFDGRL
1060 1070 1080 1090 1100
SLKERTSVLA DFAVKDYSRQ KILLLSLKAG GVGLNLTCAS HAYMMDPWWS
1110 1120 1130 1140 1150
PSMEDQAIDR LHRIGQTNSV KVMRFIIQDS IEEKMLRIQE KKRTIGEAMD
1160
TDEDERRKRR IEEIQMLFE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 478 | Q → R in AAB23590 (PubMed:1394508).Curated | 1 | |
Sequence conflicti | 635 | T → N in AAB23590 (PubMed:1394508).Curated | 1 | |
Sequence conflicti | 846 | G → S in AAB23590 (PubMed:1394508).Curated | 1 | |
Sequence conflicti | 898 | R → S in AAB23590 (PubMed:1394508).Curated | 1 | |
Sequence conflicti | 973 | V → A in AAB23590 (PubMed:1394508).Curated | 1 | |
Sequence conflicti | 1063 | A → R in AAB23590 (PubMed:1394508).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96644 Genomic DNA Translation: AAA34951.1 Z73204 Genomic DNA Translation: CAA97556.1 S46103 Genomic DNA Translation: AAB23590.1 BK006945 Genomic DNA Translation: DAA09350.1 |
PIRi | S64859 |
RefSeqi | NP_013132.1, NM_001181919.1 |
Genome annotation databases
EnsemblFungii | YLR032W_mRNA; YLR032W; YLR032W |
GeneIDi | 850719 |
KEGGi | sce:YLR032W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96644 Genomic DNA Translation: AAA34951.1 Z73204 Genomic DNA Translation: CAA97556.1 S46103 Genomic DNA Translation: AAB23590.1 BK006945 Genomic DNA Translation: DAA09350.1 |
PIRi | S64859 |
RefSeqi | NP_013132.1, NM_001181919.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5YRQ | X-ray | 2.00 | A/B/D/E | 5-20 | [»] | |
SASBDBi | P32849 | |||||
SMRi | P32849 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31306, 356 interactors |
DIPi | DIP-5830N |
IntActi | P32849, 5 interactors |
MINTi | P32849 |
STRINGi | 4932.YLR032W |
PTM databases
iPTMneti | P32849 |
Proteomic databases
MaxQBi | P32849 |
PaxDbi | P32849 |
PRIDEi | P32849 |
Genome annotation databases
EnsemblFungii | YLR032W_mRNA; YLR032W; YLR032W |
GeneIDi | 850719 |
KEGGi | sce:YLR032W |
Organism-specific databases
SGDi | S000004022, RAD5 |
VEuPathDBi | FungiDB:YLR032W |
Phylogenomic databases
eggNOGi | KOG1001, Eukaryota |
GeneTreei | ENSGT00940000165376 |
HOGENOMi | CLU_000315_2_5_1 |
InParanoidi | P32849 |
OMAi | KVVRFIM |
Miscellaneous databases
PROi | PR:P32849 |
RNActi | P32849, protein |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit 3.40.50.10810, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR014905, HIRAN IPR027417, P-loop_NTPase IPR038718, SNF2-like_sf IPR000330, SNF2_N IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF08797, HIRAN, 1 hit PF00176, SNF2-rel_dom, 1 hit PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit SM00910, HIRAN, 1 hit SM00184, RING, 1 hit |
SUPFAMi | SSF52540, SSF52540, 2 hits |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAD5_YEAST | |
Accessioni | P32849Primary (citable) accession number: P32849 Secondary accession number(s): D6VY34 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 196 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families