UniProtKB - P32847 (B3AT_ONCMY)
Band 3 anion exchange protein
slc4a1
Functioni
Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
By similarityGO - Molecular functioni
- anion:anion antiporter activity Source: UniProtKB-KW
- bicarbonate transmembrane transporter activity Source: UniProtKB
- inorganic anion exchanger activity Source: UniProtKB
GO - Biological processi
- bicarbonate transport Source: UniProtKB
Keywordsi
Biological process | Anion exchange, Ion transport, Transport |
Protein family/group databases
TCDBi | 2.A.31.1.4, the anion exchanger (ae) family |
Names & Taxonomyi
Protein namesi | Recommended name: Band 3 anion exchange proteinAlternative name(s): Anion exchange protein 1 Short name: AE 1 Short name: Anion exchanger 1 Solute carrier family 4 member 1 |
Gene namesi | Name:slc4a1 Synonyms:ae1 |
Organismi | Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) |
Taxonomic identifieri | 8022 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Protacanthopterygii › Salmoniformes › Salmonidae › Salmoninae › Oncorhynchus |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 392 | CytoplasmicBy similarityAdd BLAST | 392 | |
Transmembranei | 393 – 416 | Helical; Name=1By similarityAdd BLAST | 24 | |
Topological domaini | 417 – 424 | ExtracellularBy similarity | 8 | |
Transmembranei | 425 – 445 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 446 – 448 | CytoplasmicBy similarity | 3 | |
Transmembranei | 449 – 465 | Discontinuously helical; Name=3By similarityAdd BLAST | 17 | |
Topological domaini | 466 – 474 | ExtracellularBy similarity | 9 | |
Transmembranei | 475 – 495 | Helical; Name=4By similarityAdd BLAST | 21 | |
Topological domaini | 496 – 507 | CytoplasmicBy similarityAdd BLAST | 12 | |
Transmembranei | 508 – 530 | Helical; Name=5By similarityAdd BLAST | 23 | |
Topological domaini | 531 – 583 | ExtracellularBy similarityAdd BLAST | 53 | |
Transmembranei | 584 – 604 | Helical; Name=6By similarityAdd BLAST | 21 | |
Topological domaini | 605 – 615 | CytoplasmicBy similarityAdd BLAST | 11 | |
Transmembranei | 616 – 636 | Helical; Name=7By similarityAdd BLAST | 21 | |
Topological domaini | 637 – 676 | ExtracellularBy similarityAdd BLAST | 40 | |
Transmembranei | 677 – 697 | Helical; Name=8By similarityAdd BLAST | 21 | |
Topological domaini | 698 – 713 | CytoplasmicBy similarityAdd BLAST | 16 | |
Transmembranei | 714 – 732 | Helical; Name=9By similarityAdd BLAST | 19 | |
Transmembranei | 733 – 750 | Discontinuously helical; Name=10By similarityAdd BLAST | 18 | |
Topological domaini | 751 – 769 | CytoplasmicBy similarityAdd BLAST | 19 | |
Transmembranei | 770 – 790 | Helical; Name=11By similarityAdd BLAST | 21 | |
Transmembranei | 791 – 809 | Helical; Name=12By similarityAdd BLAST | 19 | |
Topological domaini | 810 – 847 | CytoplasmicBy similarityAdd BLAST | 38 | |
Intramembranei | 848 – 878 | Discontinuously helicalBy similarityAdd BLAST | 31 | |
Topological domaini | 879 – 918 | CytoplasmicBy similarityAdd BLAST | 40 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079213 | 1 – 918 | Band 3 anion exchange proteinAdd BLAST | 918 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 546 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 568 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Lipidationi | 852 | S-palmitoyl cysteineBy similarity | 1 |
Keywords - PTMi
Glycoprotein, Lipoprotein, PalmitateInteractioni
Subunit structurei
A dimer in solution, it spans the membrane asymmetrically and appears to be tetrameric.
By similarityFamily & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 48 | DisorderedSequence analysisAdd BLAST | 48 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 16 | Acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
OrthoDBi | 265068at2759 |
Family and domain databases
Gene3Di | 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR001717, Anion_exchange IPR002977, Anion_exchange_1 IPR018241, Anion_exchange_CS IPR013769, Band3_cytoplasmic_dom IPR011531, HCO3_transpt-like_TM_dom IPR003020, HCO3_transpt_euk IPR016152, PTrfase/Anion_transptr |
PANTHERi | PTHR11453, PTHR11453, 1 hit |
Pfami | View protein in Pfam PF07565, Band_3_cyto, 2 hits PF00955, HCO3_cotransp, 1 hit |
PRINTSi | PR00165, ANIONEXCHNGR PR01187, ANIONEXHNGR1 PR01231, HCO3TRNSPORT |
SUPFAMi | SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00834, ae, 1 hit |
PROSITEi | View protein in PROSITE PS00219, ANION_EXCHANGER_1, 1 hit PS00220, ANION_EXCHANGER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MENDLSFGED VMSYEEESDS AFPSPIRPTP PGHSGNYDLE QSRQEEDSNQ
60 70 80 90 100
AIQSIVVHTD PEAYLNLNTN ANTRGDAQAY VELNELMGNS WQETGRWVGY
110 120 130 140 150
EENFNPGTGK WGPSHVSYLT FKSLIQLRKI MSTGAIILDL QASSLSAVAE
160 170 180 190 200
KVVDELRTKG EIRAADRDGL LRALLQRRSQ SEGAVAQPLG GDIEMQTFSV
210 220 230 240 250
TKQRDTTDSV EASIVLSGVM DSLEKPAVAF VRLGDSVVIE GALEAPVPVR
260 270 280 290 300
FVFVLVGPSQ GGVDYHESGR AMAALMADWV FSLEAYLAPT NKELTNAIAD
310 320 330 340 350
FMDCGIVIPP TEIQDEGMLQ PIIDFQKKML KDRLRPSDTR IIFGGGAKAD
360 370 380 390 400
EADEEPREDP LARTGIPFGG MIKDMKRRYR HYISDFTDAL DPQVLAAVIF
410 420 430 440 450
IYFAALSPAI TFGGLLADKT EHMMGVSELM ISTCVQGIIF AFIAAQPTLV
460 470 480 490 500
IGFSGPLLVF EEAFFAFCKS QEIEYIVGRI WVGLWLVIIV VVIVAVEGSF
510 520 530 540 550
LVKFISRFTQ EIFSILISLI FIYETFSKLG KIFKAHPLVL NYEHLNDSLD
560 570 580 590 600
NPFHPVVKEH IEYHEDGNKT VHEVIHERAY PNTALLSMCL MFGCFFIAYF
610 620 630 640 650
LRQFKNGHFL PGPIRRMIGD FGVPIAIFFM IAVDITIEDA YTQKLVVPKG
660 670 680 690 700
LMVSNPNARG WFINPLGEKK PFPAWMMGAC CVPALLVFIL IFLESQITTL
710 720 730 740 750
IVSKPERKMV KGSGFHLDLL ILVTMGGIAS LFGVPWLSAA TVRSVTHANA
760 770 780 790 800
LTVMSKGPKP EIEKVLEQRI SGMLVAAMVG VSILLEPILK MIPMTALFGI
810 820 830 840 850
FLYMGITSLS GIQMWDRMLL LIVPRKYYPA DAYAQRVTTM KMHLFTLIQM
860 870 880 890 900
VCLGALWMVK MSAFSLALPF VLILTIPLRM AITGTLFTDK EMKCLDASDG
910
KVKFEEEPGE DMYESPLP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61699 mRNA Translation: CAA43868.1 |
PIRi | S24318 |
RefSeqi | NP_001118189.1, NM_001124717.1 |
Genome annotation databases
GeneIDi | 100136769 |
KEGGi | omy:100136769 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X61699 mRNA Translation: CAA43868.1 |
PIRi | S24318 |
RefSeqi | NP_001118189.1, NM_001124717.1 |
3D structure databases
AlphaFoldDBi | P32847 |
SMRi | P32847 |
ModBasei | Search... |
Protein family/group databases
TCDBi | 2.A.31.1.4, the anion exchanger (ae) family |
Genome annotation databases
GeneIDi | 100136769 |
KEGGi | omy:100136769 |
Phylogenomic databases
OrthoDBi | 265068at2759 |
Family and domain databases
Gene3Di | 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR001717, Anion_exchange IPR002977, Anion_exchange_1 IPR018241, Anion_exchange_CS IPR013769, Band3_cytoplasmic_dom IPR011531, HCO3_transpt-like_TM_dom IPR003020, HCO3_transpt_euk IPR016152, PTrfase/Anion_transptr |
PANTHERi | PTHR11453, PTHR11453, 1 hit |
Pfami | View protein in Pfam PF07565, Band_3_cyto, 2 hits PF00955, HCO3_cotransp, 1 hit |
PRINTSi | PR00165, ANIONEXCHNGR PR01187, ANIONEXHNGR1 PR01231, HCO3TRNSPORT |
SUPFAMi | SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00834, ae, 1 hit |
PROSITEi | View protein in PROSITE PS00219, ANION_EXCHANGER_1, 1 hit PS00220, ANION_EXCHANGER_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | B3AT_ONCMY | |
Accessioni | P32847Primary (citable) accession number: P32847 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | February 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 107 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |