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UniProtKB - P32833 (ORC2_YEAST)
Protein
Origin recognition complex subunit 2
Gene
ORC2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication.
1 PublicationMiscellaneous
Present with 1700 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- chromatin binding Source: SGD
- DNA replication origin binding Source: SGD
GO - Biological processi
- DNA replication initiation Source: SGD
- histone H3-K4 methylation Source: SGD
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
- silent mating-type cassette heterochromatin assembly Source: SGD
- subtelomeric heterochromatin assembly Source: SGD
Keywordsi
Biological process | DNA replication |
Enzyme and pathway databases
Reactomei | R-SCE-68616, Assembly of the ORC complex at the origin of replication R-SCE-68949, Orc1 removal from chromatin R-SCE-68962, Activation of the pre-replicative complex |
Names & Taxonomyi
Protein namesi | Recommended name: Origin recognition complex subunit 2Alternative name(s): Origin recognition complex 71 kDa subunit |
Gene namesi | Name:ORC2 Synonyms:RRR1, SIR5 Ordered Locus Names:YBR060C ORF Names:YBR0523 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000264, ORC2 |
VEuPathDBi | FungiDB:YBR060C |
Subcellular locationi
Nucleus
Nucleus
- DNA replication preinitiation complex Source: SGD
- nuclear origin of replication recognition complex Source: SGD
- nuclear pre-replicative complex Source: SGD
- nucleus Source: ComplexPortal
Other locations
- chromosome, telomeric region Source: GOC
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127082 | 1 – 620 | Origin recognition complex subunit 2Add BLAST | 620 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 60 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 187 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 188 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P32833 |
PaxDbi | P32833 |
PRIDEi | P32833 |
TopDownProteomicsi | P32833 |
PTM databases
iPTMneti | P32833 |
Interactioni
Subunit structurei
Component of the origin recognition complex (ORC) composed of at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6.
Interacts with MCM10 and TAH11.
2 PublicationsBinary interactionsi
P32833
With | #Exp. | IntAct |
---|---|---|
ORC3 [P54790] | 6 | EBI-12572,EBI-12576 |
ORC5 [P50874] | 4 | EBI-12572,EBI-12584 |
ORC6 [P38826] | 6 | EBI-12572,EBI-12588 |
Protein-protein interaction databases
BioGRIDi | 32764, 691 interactors |
ComplexPortali | CPX-768, Nuclear origin recognition complex |
DIPi | DIP-2285N |
IntActi | P32833, 17 interactors |
MINTi | P32833 |
STRINGi | 4932.YBR060C |
Miscellaneous databases
RNActi | P32833, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P32833 |
SMRi | P32833 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 184 | DisorderedSequence analysisAdd BLAST | 184 | |
Regioni | 204 – 225 | DisorderedSequence analysisAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 24 – 45 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 80 – 116 | Basic and acidic residuesSequence analysisAdd BLAST | 37 | |
Compositional biasi | 130 – 146 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 147 – 167 | Polar residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Belongs to the ORC2 family.Curated
Phylogenomic databases
eggNOGi | KOG2928, Eukaryota |
GeneTreei | ENSGT00390000015228 |
HOGENOMi | CLU_022671_0_0_1 |
InParanoidi | P32833 |
OMAi | FIEHKMA |
Family and domain databases
InterProi | View protein in InterPro IPR007220, ORC2 |
PANTHERi | PTHR14052, PTHR14052, 1 hit |
Pfami | View protein in Pfam PF04084, ORC2, 1 hit |
i Sequence
Sequence statusi: Complete.
P32833-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLNGEDFVEH NDILSSPAKS RNVTPKRVDP HGERQLRRIH SSKKNLLERI
60 70 80 90 100
SLVGNERKNT SPDPALKPKT PSKAPRKRGR PRKIQEELTD RIKKDEKDTI
110 120 130 140 150
SSKKKRKLDK DTSGNVNEES KTSNNKQVME KTGIKEKRER EKIQVATTTY
160 170 180 190 200
EDNVTPQTDD NFVSNSPEPP EPATPSKKSL TTNHDFTSPL KQIIMNNLKE
210 220 230 240 250
YKDSTSPGKL TLSRNFTPTP VPKNKKLYQT SETKSASSFL DTFEGYFDQR
260 270 280 290 300
KIVRTNAKSR HTMSMAPDVT REEFSLVSNF FNENFQKRPR QKLFEIQKKM
310 320 330 340 350
FPQYWFELTQ GFSLLFYGVG SKRNFLEEFA IDYLSPKIAY SQLAYENELQ
360 370 380 390 400
QNKPVNSIPC LILNGYNPSC NYRDVFKEIT DLLVPAELTR SETKYWGNHV
410 420 430 440 450
ILQIQKMIDF YKNQPLDIKL ILVVHNLDGP SIRKNTFQTM LSFLSVIRQI
460 470 480 490 500
AIVASTDHIY APLLWDNMKA QNYNFVFHDI SNFEPSTVES TFQDVMKMGK
510 520 530 540 550
SDTSSGAEGA KYVLQSLTVN SKKMYKLLIE TQMQNMGNLS ANTGPKRGTQ
560 570 580 590 600
RTGVELKLFN HLCAADFIAS NEIALRSMLR EFIEHKMANI TKNNSGMEII
610 620
WVPYTYAELE KLLKTVLNTL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L23924 Unassigned DNA Translation: AAA16325.1 Z21817 Genomic DNA Translation: CAA79883.1 Z35929 Genomic DNA Translation: CAA85003.1 BK006936 Genomic DNA Translation: DAA07180.1 |
PIRi | S38994 |
RefSeqi | NP_009616.1, NM_001178408.1 |
Genome annotation databases
EnsemblFungii | YBR060C_mRNA; YBR060C; YBR060C |
GeneIDi | 852352 |
KEGGi | sce:YBR060C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L23924 Unassigned DNA Translation: AAA16325.1 Z21817 Genomic DNA Translation: CAA79883.1 Z35929 Genomic DNA Translation: CAA85003.1 BK006936 Genomic DNA Translation: DAA07180.1 |
PIRi | S38994 |
RefSeqi | NP_009616.1, NM_001178408.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5V8F | electron microscopy | 3.90 | B | 1-620 | [»] | |
5ZR1 | electron microscopy | 3.00 | B | 1-620 | [»] | |
6RQC | electron microscopy | 4.40 | B | 1-620 | [»] | |
6WGC | electron microscopy | 4.30 | B | 1-620 | [»] | |
6WGG | electron microscopy | 8.10 | B | 1-620 | [»] | |
6WGI | electron microscopy | 10.00 | B | 1-620 | [»] | |
7MCA | electron microscopy | 3.60 | B | 1-620 | [»] | |
AlphaFoldDBi | P32833 | |||||
SMRi | P32833 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32764, 691 interactors |
ComplexPortali | CPX-768, Nuclear origin recognition complex |
DIPi | DIP-2285N |
IntActi | P32833, 17 interactors |
MINTi | P32833 |
STRINGi | 4932.YBR060C |
PTM databases
iPTMneti | P32833 |
Proteomic databases
MaxQBi | P32833 |
PaxDbi | P32833 |
PRIDEi | P32833 |
TopDownProteomicsi | P32833 |
Genome annotation databases
EnsemblFungii | YBR060C_mRNA; YBR060C; YBR060C |
GeneIDi | 852352 |
KEGGi | sce:YBR060C |
Organism-specific databases
SGDi | S000000264, ORC2 |
VEuPathDBi | FungiDB:YBR060C |
Phylogenomic databases
eggNOGi | KOG2928, Eukaryota |
GeneTreei | ENSGT00390000015228 |
HOGENOMi | CLU_022671_0_0_1 |
InParanoidi | P32833 |
OMAi | FIEHKMA |
Enzyme and pathway databases
Reactomei | R-SCE-68616, Assembly of the ORC complex at the origin of replication R-SCE-68949, Orc1 removal from chromatin R-SCE-68962, Activation of the pre-replicative complex |
Miscellaneous databases
PROi | PR:P32833 |
RNActi | P32833, protein |
Family and domain databases
InterProi | View protein in InterPro IPR007220, ORC2 |
PANTHERi | PTHR14052, PTHR14052, 1 hit |
Pfami | View protein in Pfam PF04084, ORC2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ORC2_YEAST | |
Accessioni | P32833Primary (citable) accession number: P32833 Secondary accession number(s): D6VQ60 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | February 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families