UniProtKB - P32807 (KU70_YEAST)
ATP-dependent DNA helicase II subunit 1
YKU70
Functioni
Single-stranded DNA-dependent ATP-dependent helicase. Involved in non-homologous end joining (NHEJ) DNA double strand break repair. DNA-binding is sequence-independent but has a high affinity to nicks in double-stranded DNA and to the ends of duplex DNA. Binds to naturally occurring chromosomal ends, and therefore provides chromosomal end protection. Appears to have a role in recruitment of telomerase and CDC13 to the telomere and the subsequent telomere elongation. Required also for telomere recombination to repair telomeric ends in the absence of telomerase. KU70, of the KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA component of telomerase. Involved in telomere maintenance. Interacts with telomeric repeats and subtelomeric sequences thereby controlling telomere length and protecting against subtelomeric rearrangement. Maintains telomeric chromatin, which is involved in silencing the expression of genes located at the telomere. Required for mating-type switching.
11 PublicationsMiscellaneous
Catalytic activityi
- EC:3.6.4.12
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- damaged DNA binding Source: InterPro
- DNA helicase activity Source: UniProtKB-EC
- telomerase RNA binding Source: SGD
- telomeric DNA binding Source: GO_Central
GO - Biological processi
- cellular response to gamma radiation Source: GO_Central
- cellular response to X-ray Source: GO_Central
- chromatin assembly or disassembly Source: SGD
- double-strand break repair via break-induced replication Source: SGD
- double-strand break repair via homologous recombination Source: SGD
- double-strand break repair via nonhomologous end joining Source: SGD
- establishment of protein-containing complex localization to telomere Source: SGD
- mitochondrial double-strand break repair via homologous recombination Source: SGD
- recombinational repair Source: ComplexPortal
- silent mating-type cassette heterochromatin assembly Source: SGD
- subtelomeric heterochromatin assembly Source: SGD
- telomere maintenance Source: SGD
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA damage, DNA recombination, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent DNA helicase II subunit 1 (EC:3.6.4.12)Alternative name(s): ATP-dependent DNA helicase II subunit Ku70 High affinity DNA-binding factor subunit 1 |
Gene namesi | Name:YKU70 Synonyms:HDF1, NES24 Ordered Locus Names:YMR284W ORF Names:YM8021.10 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004897, YKU70 |
VEuPathDBi | FungiDB:YMR284W |
Subcellular locationi
Nucleus
- Ku70:Ku80 complex Source: SGD
- nuclear envelope Source: SGD
Other locations
- chromosome, telomeric region Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chromosome, Nucleus, TelomerePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000210184 | 1 – 602 | ATP-dependent DNA helicase II subunit 1Add BLAST | 602 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 370 | PhosphoserineCombined sources | 1 | |
Modified residuei | 371 | PhosphoserineCombined sources | 1 | |
Modified residuei | 372 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P32807 |
PaxDbi | P32807 |
PRIDEi | P32807 |
PTM databases
iPTMneti | P32807 |
Interactioni
Subunit structurei
Heterodimer of YKU70/HDF1 and YKU80/HDF2.
1 PublicationBinary interactionsi
P32807
With | #Exp. | IntAct |
---|---|---|
SMT3 [Q12306] | 2 | EBI-8214,EBI-17490 |
YKU80 [Q04437] | 2 | EBI-8214,EBI-8224 |
Protein-protein interaction databases
BioGRIDi | 35464, 365 interactors |
ComplexPortali | CPX-1732, Ku70:Ku80 complex |
DIPi | DIP-2483N |
IntActi | P32807, 28 interactors |
MINTi | P32807 |
STRINGi | 4932.YMR284W |
Miscellaneous databases
RNActi | P32807, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P32807 |
SMRi | P32807 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 268 – 483 | KuAdd BLAST | 216 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2327, Eukaryota |
GeneTreei | ENSGT00940000153239 |
HOGENOMi | CLU_024202_0_0_1 |
InParanoidi | P32807 |
OMAi | PNDMMGI |
Family and domain databases
Gene3Di | 2.40.290.10, 1 hit 3.40.50.410, 1 hit |
InterProi | View protein in InterPro IPR006165, Ku70 IPR006164, Ku70/Ku80_beta-barrel_dom IPR005160, Ku_C IPR005161, Ku_N IPR016194, SPOC-like_C_dom_sf IPR036465, vWFA_dom_sf |
PANTHERi | PTHR12604:SF2, PTHR12604:SF2, 1 hit |
Pfami | View protein in Pfam PF02735, Ku, 1 hit PF03730, Ku_C, 1 hit PF03731, Ku_N, 1 hit |
PIRSFi | PIRSF003033, Ku70, 1 hit |
SMARTi | View protein in SMART SM00559, Ku78, 1 hit |
SUPFAMi | SSF100939, SSF100939, 1 hit SSF53300, SSF53300, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MRSVTNAFGN SGELNDQVDE TGYRKFDIHE GILFCIELSE TMFKESSDLE
60 70 80 90 100
YKSPLLEILE SLDELMSQLV ITRPGTAIGC YFYYCNREDA KEGIYELFPL
110 120 130 140 150
RDINATFMKK LNDLLEDLSS GRISLYDYFM FQQTGSEKQV RLSVLFTFML
160 170 180 190 200
DTFLEEIPGQ KQLSNKRVFL FTDIDKPQEA QDIDERARLR RLTIDLFDNK
210 220 230 240 250
VNFATFFIGY ADKPFDNEFY SDILQLGSHT NENTGLDSEF DGPSTKPIDA
260 270 280 290 300
KYIKSRILRK KEVKRIMFQC PLILDEKTNF IVGVKGYTMY THEKAGVRYK
310 320 330 340 350
LVYEHEDIRQ EAYSKRKFLN PITGEDVTGK TVKVYPYGDL DINLSDSQDQ
360 370 380 390 400
IVMEAYTQKD AFLKIIGFRS SSKSIHYFNN IDKSSFIVPD EAKYEGSIRT
410 420 430 440 450
LASLLKILRK KDKIAILWGK LKSNSHPSLY TLSPSSVKDY NEGFYLYRVP
460 470 480 490 500
FLDEIRKFPS LLSYDDGSEH KLDYDNMKKV TQSIMGYFNL RDGYNPSDFK
510 520 530 540 550
NPLLQKHYKV LHDYLLQIET TFDENETPNT KKDRMMREDD SLRKLYYIRN
560 570 580 590 600
KILESEKSED PIIQRLNKYV KIWNMFYKKF NDDNISIKEE KKPFDKKPKF
NI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 | S → P in CAA49840 (PubMed:8509423).Curated | 1 | |
Sequence conflicti | 87 | R → T in AAT92882 (PubMed:9169872).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 21 | T → K in strain: ABYS 60 and SK1. | 1 | |
Natural varianti | 50 | E → D in strain: ABYS 60, DBVPG6044, SK1 and YPS128. | 1 | |
Natural varianti | 230 | T → A in strain: DBVPG6044. | 1 | |
Natural varianti | 368 | F → I in strain: YPS128. | 1 | |
Natural varianti | 562 | I → T in strain: ABYS 60, DBVPG6044, SK1 and YPS128. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X70379 Genomic DNA Translation: CAA49840.1 D15052 Genomic DNA Translation: BAA03648.1 AM296304 Genomic DNA Translation: CAL36013.1 AM296302 Genomic DNA Translation: CAL36015.1 AM296301 Genomic DNA Translation: CAL36016.1 AM296299 Genomic DNA Translation: CAL36018.1 AM296298 Genomic DNA Translation: CAL36019.1 AM296297 Genomic DNA Translation: CAL36020.1 AM296296 Genomic DNA Translation: CAL36021.1 AM296294 Genomic DNA Translation: CAL36023.1 AM296292 Genomic DNA Translation: CAL36025.1 AM296295 Genomic DNA Translation: CAL36022.1 AM296303 Genomic DNA Translation: CAL36014.1 AM296293 Genomic DNA Translation: CAL36024.1 Z49704 Genomic DNA Translation: CAA89782.1 AY692863 Genomic DNA Translation: AAT92882.1 BK006946 Genomic DNA Translation: DAA10185.1 |
PIRi | S54591 |
RefSeqi | NP_014011.1, NM_001182791.1 |
Genome annotation databases
EnsemblFungii | YMR284W_mRNA; YMR284W; YMR284W |
GeneIDi | 855328 |
KEGGi | sce:YMR284W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X70379 Genomic DNA Translation: CAA49840.1 D15052 Genomic DNA Translation: BAA03648.1 AM296304 Genomic DNA Translation: CAL36013.1 AM296302 Genomic DNA Translation: CAL36015.1 AM296301 Genomic DNA Translation: CAL36016.1 AM296299 Genomic DNA Translation: CAL36018.1 AM296298 Genomic DNA Translation: CAL36019.1 AM296297 Genomic DNA Translation: CAL36020.1 AM296296 Genomic DNA Translation: CAL36021.1 AM296294 Genomic DNA Translation: CAL36023.1 AM296292 Genomic DNA Translation: CAL36025.1 AM296295 Genomic DNA Translation: CAL36022.1 AM296303 Genomic DNA Translation: CAL36014.1 AM296293 Genomic DNA Translation: CAL36024.1 Z49704 Genomic DNA Translation: CAA89782.1 AY692863 Genomic DNA Translation: AAT92882.1 BK006946 Genomic DNA Translation: DAA10185.1 |
PIRi | S54591 |
RefSeqi | NP_014011.1, NM_001182791.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5Y58 | X-ray | 2.80 | A/C/E | 28-602 | [»] | |
AlphaFoldDBi | P32807 | |||||
SMRi | P32807 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35464, 365 interactors |
ComplexPortali | CPX-1732, Ku70:Ku80 complex |
DIPi | DIP-2483N |
IntActi | P32807, 28 interactors |
MINTi | P32807 |
STRINGi | 4932.YMR284W |
PTM databases
iPTMneti | P32807 |
Proteomic databases
MaxQBi | P32807 |
PaxDbi | P32807 |
PRIDEi | P32807 |
Genome annotation databases
EnsemblFungii | YMR284W_mRNA; YMR284W; YMR284W |
GeneIDi | 855328 |
KEGGi | sce:YMR284W |
Organism-specific databases
SGDi | S000004897, YKU70 |
VEuPathDBi | FungiDB:YMR284W |
Phylogenomic databases
eggNOGi | KOG2327, Eukaryota |
GeneTreei | ENSGT00940000153239 |
HOGENOMi | CLU_024202_0_0_1 |
InParanoidi | P32807 |
OMAi | PNDMMGI |
Enzyme and pathway databases
Reactomei | R-SCE-6798695, Neutrophil degranulation |
Miscellaneous databases
PROi | PR:P32807 |
RNActi | P32807, protein |
Family and domain databases
Gene3Di | 2.40.290.10, 1 hit 3.40.50.410, 1 hit |
InterProi | View protein in InterPro IPR006165, Ku70 IPR006164, Ku70/Ku80_beta-barrel_dom IPR005160, Ku_C IPR005161, Ku_N IPR016194, SPOC-like_C_dom_sf IPR036465, vWFA_dom_sf |
PANTHERi | PTHR12604:SF2, PTHR12604:SF2, 1 hit |
Pfami | View protein in Pfam PF02735, Ku, 1 hit PF03730, Ku_C, 1 hit PF03731, Ku_N, 1 hit |
PIRSFi | PIRSF003033, Ku70, 1 hit |
SMARTi | View protein in SMART SM00559, Ku78, 1 hit |
SUPFAMi | SSF100939, SSF100939, 1 hit SSF53300, SSF53300, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KU70_YEAST | |
Accessioni | P32807Primary (citable) accession number: P32807 Secondary accession number(s): D6W0B1 Q6B267 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | February 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 190 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families