UniProtKB - P32797 (CDC13_YEAST)
Protein
Cell division control protein 13
Gene
CDC13
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Single-stranded telomeric DNA-binding protein that regulates telomere replication. Has a role in both positive and negative regulation. Promotes [TG1-3] strand lengthening via interaction with EST1. Promotes [C1-3A] strand re-synthesis by DNA polymerase alpha via interaction with POL1. Negatively regulates telomere elongation of the G strand via binding with STN1 thereby inhibiting telomerase activity.3 Publications
Miscellaneous
Present with 319 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 500 – 686 | OBAdd BLAST | 187 |
GO - Molecular functioni
- G-rich strand telomeric DNA binding Source: GO_Central
- identical protein binding Source: IntAct
- single-stranded telomeric DNA binding Source: SGD
- telomerase inhibitor activity Source: SGD
- translation elongation factor binding Source: SGD
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- negative regulation of telomerase activity Source: SGD
- regulation of telomere maintenance via telomerase Source: SGD
- ribonucleoprotein complex localization Source: SGD
- telomere capping Source: SGD
- telomere maintenance Source: SGD
- telomere maintenance via telomerase Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, Cell division |
Names & Taxonomyi
Protein namesi | Recommended name: Cell division control protein 13 |
Gene namesi | Name:CDC13 Ordered Locus Names:YDL220C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002379, CDC13 |
VEuPathDBi | FungiDB:YDL220C |
Subcellular locationi
Other locations
Nucleus
- CST complex Source: SGD
- nuclear telomere cap complex Source: GO_Central
Other locations
- chromosome, telomeric region Source: SGD
Keywords - Cellular componenti
Chromosome, TelomerePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 50 | K → Q: Increase in length of X' and Y' telomeres. Disrupts interaction with POL1 but not FUN12. 1 Publication | 1 | |
Mutagenesisi | 72 | I → T: Disrupts interaction with POL1 and FUN12. 1 Publication | 1 | |
Mutagenesisi | 124 | C → R: Disrupts interaction with POL1 but not FUN12. 1 Publication | 1 | |
Mutagenesisi | 149 | L → S: Disrupts interaction with POL1 but not FUN12. 1 Publication | 1 | |
Mutagenesisi | 228 | S → P: Disrupts interaction with POL1 but not FUN12. 1 Publication | 1 | |
Mutagenesisi | 243 | G → R: Disrupts interaction with POL1 and FUN12. 1 Publication | 1 | |
Mutagenesisi | 392 | L → P: Disrupts interaction with POL1 but not FUN12. 1 Publication | 1 | |
Mutagenesisi | 523 | I → V: Increase in length of X' and Y' telomeres. Disrupts interaction with POL1 but not FUN12. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089441 | 1 – 924 | Cell division control protein 13Add BLAST | 924 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 306 | PhosphoserineCombined sources | 1 | |
Modified residuei | 308 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 333 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P32797 |
PaxDbi | P32797 |
PRIDEi | P32797 |
PTM databases
iPTMneti | P32797 |
Interactioni
Subunit structurei
Interacts with POL1, EST1, FUN12, STM1, STN1 and TEN1.
7 PublicationsBinary interactionsi
Hide detailsP32797
With | #Exp. | IntAct |
---|---|---|
itself | 4 | EBI-4187,EBI-4187 |
EST1 [P17214] | 3 | EBI-4187,EBI-6684 |
POL1 [P13382] | 4 | EBI-4187,EBI-6128 |
SMT3 [Q12306] | 3 | EBI-4187,EBI-17490 |
STN1 [P38960] | 10 | EBI-4187,EBI-18427 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- translation elongation factor binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 31825, 798 interactors |
ComplexPortali | CPX-15, CST complex |
DIPi | DIP-1229N |
IntActi | P32797, 26 interactors |
MINTi | P32797 |
STRINGi | 4932.YDL220C |
Miscellaneous databases
RNActi | P32797, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P32797 |
SMRi | P32797 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32797 |
Family & Domainsi
Phylogenomic databases
eggNOGi | ENOG502QU50, Eukaryota |
HOGENOMi | CLU_011303_0_0_1 |
InParanoidi | P32797 |
OMAi | ERICELF |
Family and domain databases
InterProi | View protein in InterPro IPR031749, Cdc13_N IPR040650, Cdc13_OB2 IPR041028, Cdc13_OB4_dimer IPR012340, NA-bd_OB-fold IPR028389, POT1 IPR011564, Telomer_end-bd_POT1/Cdc13 |
PANTHERi | PTHR14513, PTHR14513, 1 hit |
Pfami | View protein in Pfam PF16853, CDC13_N, 1 hit PF18691, Cdc13_OB2, 1 hit PF18233, Cdc13_OB4_dimer, 1 hit PF02765, POT1, 1 hit |
SMARTi | View protein in SMART SM00976, Telo_bind, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
i Sequence
Sequence statusi: Complete.
P32797-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDTLEEPECP PHKNRIFVSS SKDFEGYPSK AIVPVQFVAL LTSIHLTETK
60 70 80 90 100
CLLGFSNFER RGDQSQEDQY LIKLKFKDRG SERLARITIS LLCQYFDIEL
110 120 130 140 150
PDLDSDSGAS PTVILRDIHL ERLCFSSCKA LYVSKHGNYT LFLEDIKPLD
160 170 180 190 200
LVSVISTIST KSTNSSKHSS SELISECDLN NSLVDIFNNL IEMNRDEKNR
210 220 230 240 250
FKFVKLIHYD IELKKFVQDQ QKVLSQKSKA AAINPFFVPN RLGIPYIESQ
260 270 280 290 300
NEFNSQLMTL NVDEPTTDIS NMGEEMHDSA DPIEDSDSST TSSTGKYFSS
310 320 330 340 350
KSYIQSQTPE RKTSVPNNWH DDDSGSKRKR KLSFHSPNAS SIRKAISYEQ
360 370 380 390 400
LSLASVGSVE RLEGKIVGMN PPQFASINEF KYCTLKLYFT QLLPNVPDKV
410 420 430 440 450
LVPGVNCIEI VIPTRERICE LFGVLNCQSD KISDILLLEK PDRISVEVER
460 470 480 490 500
ILWDNDKTAS PGMAVWSLKN ISTDTQAQAQ VQVPAQSSAS IDPSRTRMSK
510 520 530 540 550
MARKDPTIEF CQLGLDTFET KYITMFGMLV SCSFDKPAFI SFVFSDFTKN
560 570 580 590 600
DIVQNYLYDR YLIDYENKLE LNEGFKAIMY KNQFETFDSK LRKIFNNGLR
610 620 630 640 650
DLQNGRDENL SQYGIVCKMN IKVKMYNGKL NAIVRECEPV PHSQISSIAS
660 670 680 690 700
PSQCEHLRLF YQRAFKRIGE SAISRYFEEY RRFFPIHRNG SHLAKLRFDE
710 720 730 740 750
VKHEPKKSPT TPALAEHIPD LNADVSSFDV KFTDISSLLD SSARLPRPQQ
760 770 780 790 800
THKSNTLYSC EGRIIAIEYH ASDLCFHITN ELPLLQTRGL APERVLQLHI
810 820 830 840 850
ITSKNFAYFF NRSSAYLQRQ PLEEKYTQLA QFLGHSFKFN ITSSLTLFPD
860 870 880 890 900
TTVALQIWCP IECTFRELQQ QLAHPKVAAA PDSGSLDCAI NATVNPLRLL
910 920
AAQNGVTVKK EEDNDDDAGA VPTS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 40 | L → A in AAA99990 (PubMed:7565765).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76550 Genomic DNA Translation: AAA99990.1 Z74269 Genomic DNA Translation: CAA98800.1 BK006938 Genomic DNA Translation: DAA11644.1 |
PIRi | S67783, S27421 |
RefSeqi | NP_010061.1, NM_001180280.1 |
Genome annotation databases
EnsemblFungii | YDL220C_mRNA; YDL220C; YDL220C |
GeneIDi | 851306 |
KEGGi | sce:YDL220C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76550 Genomic DNA Translation: AAA99990.1 Z74269 Genomic DNA Translation: CAA98800.1 BK006938 Genomic DNA Translation: DAA11644.1 |
PIRi | S67783, S27421 |
RefSeqi | NP_010061.1, NM_001180280.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KXL | NMR | - | A | 497-694 | [»] | |
1S40 | NMR | - | A | 497-694 | [»] | |
3NWS | X-ray | 2.50 | A/B/C/D | 13-227 | [»] | |
3NWT | X-ray | 2.70 | A | 13-227 | [»] | |
3OIP | X-ray | 2.50 | A | 12-243 | [»] | |
3OIQ | X-ray | 2.40 | A | 12-243 | [»] | |
4HCE | X-ray | 2.30 | A/B | 344-495 | [»] | |
BMRBi | P32797 | |||||
SMRi | P32797 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31825, 798 interactors |
ComplexPortali | CPX-15, CST complex |
DIPi | DIP-1229N |
IntActi | P32797, 26 interactors |
MINTi | P32797 |
STRINGi | 4932.YDL220C |
PTM databases
iPTMneti | P32797 |
Proteomic databases
MaxQBi | P32797 |
PaxDbi | P32797 |
PRIDEi | P32797 |
Genome annotation databases
EnsemblFungii | YDL220C_mRNA; YDL220C; YDL220C |
GeneIDi | 851306 |
KEGGi | sce:YDL220C |
Organism-specific databases
SGDi | S000002379, CDC13 |
VEuPathDBi | FungiDB:YDL220C |
Phylogenomic databases
eggNOGi | ENOG502QU50, Eukaryota |
HOGENOMi | CLU_011303_0_0_1 |
InParanoidi | P32797 |
OMAi | ERICELF |
Miscellaneous databases
EvolutionaryTracei | P32797 |
PROi | PR:P32797 |
RNActi | P32797, protein |
Family and domain databases
InterProi | View protein in InterPro IPR031749, Cdc13_N IPR040650, Cdc13_OB2 IPR041028, Cdc13_OB4_dimer IPR012340, NA-bd_OB-fold IPR028389, POT1 IPR011564, Telomer_end-bd_POT1/Cdc13 |
PANTHERi | PTHR14513, PTHR14513, 1 hit |
Pfami | View protein in Pfam PF16853, CDC13_N, 1 hit PF18691, Cdc13_OB2, 1 hit PF18233, Cdc13_OB4_dimer, 1 hit PF02765, POT1, 1 hit |
SMARTi | View protein in SMART SM00976, Telo_bind, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDC13_YEAST | |
Accessioni | P32797Primary (citable) accession number: P32797 Secondary accession number(s): D6VRD4, Q07650 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | July 11, 2002 | |
Last modified: | April 7, 2021 | |
This is version 179 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references