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Entry version 166 (07 Oct 2020)
Sequence version 2 (21 Feb 2006)
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Protein

Methionyl-tRNA formyltransferase, mitochondrial

Gene

FMT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Formylates methionyl-tRNA in mitochondria and the cytoplasm (PubMed:10781559, PubMed:30409808). Responsible for the formylation of the 8 N-terminally formylated (Nt-formylated) mitochondrial matrix proteins that are encoded by mitochondrial DNA (PubMed:10781559, PubMed:12549912). Nt-formylated proteins in the cytoplasm are strongly up-regulated in stationary phase or upon starvation for specific amino acids (His or Lys) and are targeted for degradation by a PSH1 E3 ubiquitin ligase-mediated fMet/N-end rule pathway. Increased Nt-formylation of cytosolic proteins appears to be important for adaptation to these stresses. Stationary phase-degraded Nt-formylated proteins include histone H3-like centromeric protein CSE4, Mediator complex subunit 3 (PGD1) and small ribosomal subunit protein uS8-A (RPS22A) (PubMed:30409808).3 Publications

Miscellaneous

Formylation of the initiator Met-tRNA is not essential for mitochondrial protein synthesis in S.cerevisiae.2 Publications
Present with 1070 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.13 sec(-1) with L-methionyl-tRNA(fMet) as substrate.1 Publication
  1. KM=0.3 µM for L-methionyl-tRNA(fMet)1 Publication

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionTransferase
    Biological processProtein biosynthesis

    Enzyme and pathway databases

    BRENDA Comprehensive Enzyme Information System

    More...
    BRENDAi
    2.1.2.9, 984

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Methionyl-tRNA formyltransferase, mitochondrial (EC:2.1.2.9)
    Short name:
    MtFMT
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:FMT1
    Ordered Locus Names:YBL013W
    ORF Names:YBL0311, YBL0313
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome II

    Organism-specific databases

    Eukaryotic Pathogen Database Resources

    More...
    EuPathDBi
    FungiDB:YBL013W

    Saccharomyces Genome Database

    More...
    SGDi
    S000000109, FMT1

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a transit peptide.<p><a href='/help/transit' target='_top'>More...</a></p>Transit peptidei1 – 26MitochondrionSequence analysisAdd BLAST26
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000001009627 – 401Methionyl-tRNA formyltransferase, mitochondrialAdd BLAST375

    <p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

    Phosphorylated by GCN2 in response to nutrient deprivation. Phosphorylation mediates retention of FMT1 in the cytoplasm.1 Publication

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P32785

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P32785

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...
    BioGRIDi
    32687, 174 interactors

    Database of interacting proteins

    More...
    DIPi
    DIP-8221N

    Protein interaction database and analysis system

    More...
    IntActi
    P32785, 3 interactors

    STRING: functional protein association networks

    More...
    STRINGi
    4932.YBL013W

    Miscellaneous databases

    RNAct, Protein-RNA interaction predictions for model organisms.

    More...
    RNActi
    P32785, protein

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni159 – 162Tetrahydrofolate (THF) bindingBy similarity4

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the Fmt family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    KOG3082, Eukaryota

    Ensembl GeneTree

    More...
    GeneTreei
    ENSGT00390000017828

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_033347_0_1_1

    KEGG Orthology (KO)

    More...
    KOi
    K00604

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    IKPEDRH

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd08646, FMT_core_Met-tRNA-FMT_N, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR005794, Fmt
    IPR005793, Formyl_trans_C
    IPR002376, Formyl_transf_N
    IPR036477, Formyl_transf_N_sf
    IPR041711, Met-tRNA-FMT_N

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF02911, Formyl_trans_C, 1 hit
    PF00551, Formyl_trans_N, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF53328, SSF53328, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR00460, fmt, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

    P32785-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MVKMRRITPT RLLFTCRYIS NNASPPVQPL NVLFFGSDTF SNFSLQALNE
    60 70 80 90 100
    LRQNNGSCGI VDNIQVVTRS PKWCGRQKSI LKYPPIFDMA EKLQLPRPIT
    110 120 130 140 150
    CDTKQEMLAL SKLTPSRQGN PENDGSGAPF NAIIAVSFGK LIPGDLIRAV
    160 170 180 190 200
    PLALNVHPSL LPRHKGSAPI QRALLEGDTY TGVTIQTLHP DRFDHGAIVA
    210 220 230 240 250
    QTEPLAIATM LSKGRVNDST ADFNSEGLPR RTAILMDQLG ALGAQLLGQT
    260 270 280 290 300
    LRERLYLPQN RVQAPTAYKP SYAHRITTED KRIHWARDSA AELLNKLETL
    310 320 330 340 350
    GPLHAFKEAT AARKDAQNSV LKRILFHECK VMRDARLDNG SKPGMFKYDD
    360 370 380 390 400
    IKDCILVTCR GNLLLCVSRL QFEGFAVERA GQFMARLRKR CGALSEKLVF

    L
    Length:401
    Mass (Da):44,617
    Last modified:February 21, 2006 - v2
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i95E1C61C4E6D3AE1
    GO

    <p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

    The sequence CAA84832 differs from that shown. Reason: Frameshift.Curated

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AY490279 Genomic DNA Translation: AAR86694.1
    AY492339 Genomic DNA Translation: AAR86695.1
    Z35774 Genomic DNA Translation: CAA84832.1 Frameshift.
    BK006936 Genomic DNA Translation: DAA07107.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    S25331

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_009540.2, NM_001178253.1

    Genome annotation databases

    Ensembl fungal genome annotation project

    More...
    EnsemblFungii
    YBL013W_mRNA; YBL013W; YBL013W

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    852270

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    sce:YBL013W

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY490279 Genomic DNA Translation: AAR86694.1
    AY492339 Genomic DNA Translation: AAR86695.1
    Z35774 Genomic DNA Translation: CAA84832.1 Frameshift.
    BK006936 Genomic DNA Translation: DAA07107.1
    PIRiS25331
    RefSeqiNP_009540.2, NM_001178253.1

    3D structure databases

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    SWISS-MODEL Interactive Workspace

    More...
    SWISS-MODEL-Workspacei
    Submit a new modelling project...

    Protein-protein interaction databases

    BioGRIDi32687, 174 interactors
    DIPiDIP-8221N
    IntActiP32785, 3 interactors
    STRINGi4932.YBL013W

    Proteomic databases

    PaxDbiP32785
    PRIDEiP32785

    Genome annotation databases

    EnsemblFungiiYBL013W_mRNA; YBL013W; YBL013W
    GeneIDi852270
    KEGGisce:YBL013W

    Organism-specific databases

    EuPathDBiFungiDB:YBL013W
    SGDiS000000109, FMT1

    Phylogenomic databases

    eggNOGiKOG3082, Eukaryota
    GeneTreeiENSGT00390000017828
    HOGENOMiCLU_033347_0_1_1
    KOiK00604
    OMAiIKPEDRH

    Enzyme and pathway databases

    BRENDAi2.1.2.9, 984

    Miscellaneous databases

    Protein Ontology

    More...
    PROi
    PR:P32785
    RNActiP32785, protein

    Family and domain databases

    CDDicd08646, FMT_core_Met-tRNA-FMT_N, 1 hit
    InterProiView protein in InterPro
    IPR005794, Fmt
    IPR005793, Formyl_trans_C
    IPR002376, Formyl_transf_N
    IPR036477, Formyl_transf_N_sf
    IPR041711, Met-tRNA-FMT_N
    PfamiView protein in Pfam
    PF02911, Formyl_trans_C, 1 hit
    PF00551, Formyl_trans_N, 1 hit
    SUPFAMiSSF53328, SSF53328, 1 hit
    TIGRFAMsiTIGR00460, fmt, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiFMT_YEAST
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P32785
    Secondary accession number(s): D6VPY7, Q6RUA6
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 21, 2006
    Last modified: October 7, 2020
    This is version 166 of the entry and version 2 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families
    UniProt is an ELIXIR core data resource
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