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Entry version 170 (29 Sep 2021)
Sequence version 2 (21 Feb 2006)
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Protein

Methionyl-tRNA formyltransferase, mitochondrial

Gene

FMT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Formylates methionyl-tRNA in mitochondria and the cytoplasm (PubMed:10781559, PubMed:30409808).

Responsible for the formylation of the 8 N-terminally formylated (Nt-formylated) mitochondrial matrix proteins that are encoded by mitochondrial DNA (PubMed:10781559, PubMed:12549912).

Nt-formylated proteins in the cytoplasm are strongly up-regulated in stationary phase or upon starvation for specific amino acids (His or Lys) and are targeted for degradation by a PSH1 E3 ubiquitin ligase-mediated fMet/N-end rule pathway. Increased Nt-formylation of cytosolic proteins appears to be important for adaptation to these stresses. Stationary phase-degraded Nt-formylated proteins include histone H3-like centromeric protein CSE4, Mediator complex subunit 3 (PGD1) and small ribosomal subunit protein uS8-A (RPS22A) (PubMed:30409808).

3 Publications

Miscellaneous

Formylation of the initiator Met-tRNA is not essential for mitochondrial protein synthesis in S.cerevisiae.2 Publications
Present with 1070 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.13 sec(-1) with L-methionyl-tRNA(fMet) as substrate.1 Publication
  1. KM=0.3 µM for L-methionyl-tRNA(fMet)1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase
Biological processProtein biosynthesis

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.1.2.9, 984

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Methionyl-tRNA formyltransferase, mitochondrial (EC:2.1.2.9)
Short name:
MtFMT
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:FMT1
Ordered Locus Names:YBL013W
ORF Names:YBL0311, YBL0313
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome II

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000000109, FMT1

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:YBL013W

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a transit peptide.<p><a href='/help/transit' target='_top'>More...</a></p>Transit peptidei1 – 26MitochondrionSequence analysisAdd BLAST26
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000001009627 – 401Methionyl-tRNA formyltransferase, mitochondrialAdd BLAST375

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated by GCN2 in response to nutrient deprivation. Phosphorylation mediates retention of FMT1 in the cytoplasm.1 Publication

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P32785

PRoteomics IDEntifications database

More...
PRIDEi
P32785

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
32687, 176 interactors

Database of interacting proteins

More...
DIPi
DIP-8221N

Protein interaction database and analysis system

More...
IntActi
P32785, 3 interactors

STRING: functional protein association networks

More...
STRINGi
4932.YBL013W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P32785, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
P32785

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni159 – 162Tetrahydrofolate (THF) bindingBy similarity4

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the Fmt family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3082, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000017828

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_033347_0_1_1

Identification of Orthologs from Complete Genome Data

More...
OMAi
DTCDVPR

Family and domain databases

Conserved Domains Database

More...
CDDi
cd08646, FMT_core_Met-tRNA-FMT_N, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR005794, Fmt
IPR005793, Formyl_trans_C
IPR002376, Formyl_transf_N
IPR036477, Formyl_transf_N_sf
IPR041711, Met-tRNA-FMT_N

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02911, Formyl_trans_C, 1 hit
PF00551, Formyl_trans_N, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF53328, SSF53328, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00460, fmt, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P32785-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVKMRRITPT RLLFTCRYIS NNASPPVQPL NVLFFGSDTF SNFSLQALNE
60 70 80 90 100
LRQNNGSCGI VDNIQVVTRS PKWCGRQKSI LKYPPIFDMA EKLQLPRPIT
110 120 130 140 150
CDTKQEMLAL SKLTPSRQGN PENDGSGAPF NAIIAVSFGK LIPGDLIRAV
160 170 180 190 200
PLALNVHPSL LPRHKGSAPI QRALLEGDTY TGVTIQTLHP DRFDHGAIVA
210 220 230 240 250
QTEPLAIATM LSKGRVNDST ADFNSEGLPR RTAILMDQLG ALGAQLLGQT
260 270 280 290 300
LRERLYLPQN RVQAPTAYKP SYAHRITTED KRIHWARDSA AELLNKLETL
310 320 330 340 350
GPLHAFKEAT AARKDAQNSV LKRILFHECK VMRDARLDNG SKPGMFKYDD
360 370 380 390 400
IKDCILVTCR GNLLLCVSRL QFEGFAVERA GQFMARLRKR CGALSEKLVF

L
Length:401
Mass (Da):44,617
Last modified:February 21, 2006 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i95E1C61C4E6D3AE1
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence CAA84832 differs from that shown. Reason: Frameshift.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AY490279 Genomic DNA Translation: AAR86694.1
AY492339 Genomic DNA Translation: AAR86695.1
Z35774 Genomic DNA Translation: CAA84832.1 Frameshift.
BK006936 Genomic DNA Translation: DAA07107.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S25331

NCBI Reference Sequences

More...
RefSeqi
NP_009540.2, NM_001178253.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YBL013W_mRNA; YBL013W; YBL013W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
852270

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YBL013W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY490279 Genomic DNA Translation: AAR86694.1
AY492339 Genomic DNA Translation: AAR86695.1
Z35774 Genomic DNA Translation: CAA84832.1 Frameshift.
BK006936 Genomic DNA Translation: DAA07107.1
PIRiS25331
RefSeqiNP_009540.2, NM_001178253.1

3D structure databases

SMRiP32785
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi32687, 176 interactors
DIPiDIP-8221N
IntActiP32785, 3 interactors
STRINGi4932.YBL013W

Proteomic databases

PaxDbiP32785
PRIDEiP32785

Genome annotation databases

EnsemblFungiiYBL013W_mRNA; YBL013W; YBL013W
GeneIDi852270
KEGGisce:YBL013W

Organism-specific databases

SGDiS000000109, FMT1
VEuPathDBiFungiDB:YBL013W

Phylogenomic databases

eggNOGiKOG3082, Eukaryota
GeneTreeiENSGT00390000017828
HOGENOMiCLU_033347_0_1_1
OMAiDTCDVPR

Enzyme and pathway databases

BRENDAi2.1.2.9, 984

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P32785
RNActiP32785, protein

Family and domain databases

CDDicd08646, FMT_core_Met-tRNA-FMT_N, 1 hit
InterProiView protein in InterPro
IPR005794, Fmt
IPR005793, Formyl_trans_C
IPR002376, Formyl_transf_N
IPR036477, Formyl_transf_N_sf
IPR041711, Met-tRNA-FMT_N
PfamiView protein in Pfam
PF02911, Formyl_trans_C, 1 hit
PF00551, Formyl_trans_N, 1 hit
SUPFAMiSSF53328, SSF53328, 1 hit
TIGRFAMsiTIGR00460, fmt, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiFMT_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P32785
Secondary accession number(s): D6VPY7, Q6RUA6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 21, 2006
Last modified: September 29, 2021
This is version 170 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families
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