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UniProtKB - P32773 (TOA1_YEAST)
Protein
Transcription initiation factor IIA large subunit
Gene
TOA1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
TFIIA is a component of the transcription machinery of RNA polymerase II and implicated in the regulation of basal transcription. Interacts with TBP (the TATA-binding protein).
GO - Molecular functioni
- RNA polymerase II core promoter sequence-specific DNA binding Source: CAFA
- TBP-class protein binding Source: CAFA
- transcription coregulator activity Source: SGD
GO - Biological processi
- positive regulation of transcription by RNA polymerase II Source: ComplexPortal
- positive regulation of transcription initiation from RNA polymerase II promoter Source: ComplexPortal
- RNA polymerase II preinitiation complex assembly Source: SGD
- transcription by RNA polymerase II Source: GO_Central
- transcription initiation from RNA polymerase II promoter Source: SGD
Keywordsi
Biological process | Transcription |
Enzyme and pathway databases
Reactomei | R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6807505, RNA polymerase II transcribes snRNA genes R-SCE-73776, RNA Polymerase II Promoter Escape R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-SCE-75953, RNA Polymerase II Transcription Initiation R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-SCE-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription initiation factor IIA large subunitShort name: TFIIA large subunit Alternative name(s): TFIIA 32 kDa subunit |
Gene namesi | Name:TOA1 Ordered Locus Names:YOR194C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005720, TOA1 |
VEuPathDBi | FungiDB:YOR194C |
Subcellular locationi
Nucleus
- nucleus Source: ComplexPortal
- transcription factor TFIIA complex Source: SGD
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000072617 | 1 – 286 | Transcription initiation factor IIA large subunitAdd BLAST | 286 |
Proteomic databases
MaxQBi | P32773 |
PaxDbi | P32773 |
PRIDEi | P32773 |
TopDownProteomicsi | P32773 |
PTM databases
iPTMneti | P32773 |
Interactioni
Subunit structurei
TFIIA is a heterodimer composed of the large TOA1 and a small TOA2 subunits.
Interacts with KAP122.
1 PublicationBinary interactionsi
P32773
With | #Exp. | IntAct |
---|---|---|
TOA2 [P32774] | 2 | EBI-19316,EBI-19323 |
GO - Molecular functioni
- TBP-class protein binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 34592, 274 interactors |
ComplexPortali | CPX-1633, Transcription factor TFIIA complex |
DIPi | DIP-2347N |
IntActi | P32773, 2 interactors |
MINTi | P32773 |
STRINGi | 4932.YOR194C |
Miscellaneous databases
RNActi | P32773, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32773 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32773 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 120 – 145 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 167 – 195 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 208 – 236 | DisorderedSequence analysisAdd BLAST | 29 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 217 – 236 | Acidic residuesSequence analysisAdd BLAST | 20 |
Sequence similaritiesi
Belongs to the TFIIA subunit 1 family.Curated
Phylogenomic databases
eggNOGi | KOG2652, Eukaryota |
GeneTreei | ENSGT00940000169791 |
HOGENOMi | CLU_030027_2_1_1 |
InParanoidi | P32773 |
OMAi | RTKARWK |
Family and domain databases
DisProti | DP00104 |
Gene3Di | 2.30.18.10, 1 hit |
InterProi | View protein in InterPro IPR004855, TFIIA_asu/bsu IPR009088, TFIIA_b-brl |
PANTHERi | PTHR12694, PTHR12694, 1 hit |
Pfami | View protein in Pfam PF03153, TFIIA, 2 hits |
SMARTi | View protein in SMART SM01371, TFIIA, 1 hit |
SUPFAMi | SSF50784, SSF50784, 1 hit |
i Sequence
Sequence statusi: Complete.
P32773-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNAEASRVY EIIVESVVNE VREDFENAGI DEQTLQDLKN IWQKKLTETK
60 70 80 90 100
VTTFSWDNQF NEGNINGVQN DLNFNLATPG VNSSEFNIKE ENTGNEGLIL
110 120 130 140 150
PNINSNNNIP HSGETNINTN TVEATNNSGA TLNTNTSGNT NADVTSQPKI
160 170 180 190 200
EVKPEIELTI NNANITTVEN IDDESEKKDD EEKEEDVEKT RKEKEQIEQV
210 220 230 240 250
KLQAKKEKRS ALLDTDEVGS ELDDSDDDYL ISEGEEDGPD ENLMLCLYDK
260 270 280
VTRTKARWKC SLKDGVVTIN RNDYTFQKAQ VEAEWV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | E → Q in AAT93242 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M85248 Unassigned DNA Translation: AAA19654.1 Z75102 Genomic DNA Translation: CAA99407.1 AY693223 Genomic DNA Translation: AAT93242.1 BK006948 Genomic DNA Translation: DAA10969.1 |
PIRi | B41810 |
RefSeqi | NP_014837.1, NM_001183613.1 |
Genome annotation databases
EnsemblFungii | YOR194C_mRNA; YOR194C; YOR194C |
GeneIDi | 854369 |
KEGGi | sce:YOR194C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M85248 Unassigned DNA Translation: AAA19654.1 Z75102 Genomic DNA Translation: CAA99407.1 AY693223 Genomic DNA Translation: AAT93242.1 BK006948 Genomic DNA Translation: DAA10969.1 |
PIRi | B41810 |
RefSeqi | NP_014837.1, NM_001183613.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NH2 | X-ray | 1.90 | B | 2-54 | [»] | |
C | 210-286 | [»] | ||||
1RM1 | X-ray | 2.50 | C | 1-286 | [»] | |
1YTF | X-ray | 2.50 | B | 2-54 | [»] | |
C | 210-286 | [»] | ||||
5FMF | electron microscopy | 6.00 | M | 2-286 | [»] | |
5FYW | electron microscopy | 4.35 | U | 1-286 | [»] | |
5FZ5 | electron microscopy | 8.80 | U | 1-286 | [»] | |
5OQJ | electron microscopy | 4.70 | U | 1-286 | [»] | |
5OQM | electron microscopy | 5.80 | U | 1-286 | [»] | |
5SVA | electron microscopy | 15.30 | d | 1-286 | [»] | |
6GYK | electron microscopy | 5.10 | U | 1-286 | [»] | |
6GYL | electron microscopy | 4.80 | U | 1-286 | [»] | |
6GYM | electron microscopy | 6.70 | U | 1-286 | [»] | |
7O4I | electron microscopy | 3.20 | U | 1-286 | [»] | |
7O4J | electron microscopy | 2.90 | U | 1-286 | [»] | |
7O72 | electron microscopy | 3.40 | U | 1-286 | [»] | |
7O73 | electron microscopy | 3.40 | U | 1-286 | [»] | |
7O75 | electron microscopy | 3.20 | U | 1-286 | [»] | |
7OH9 | electron microscopy | 3.00 | L | 1-286 | [»] | |
SMRi | P32773 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34592, 274 interactors |
ComplexPortali | CPX-1633, Transcription factor TFIIA complex |
DIPi | DIP-2347N |
IntActi | P32773, 2 interactors |
MINTi | P32773 |
STRINGi | 4932.YOR194C |
PTM databases
iPTMneti | P32773 |
Proteomic databases
MaxQBi | P32773 |
PaxDbi | P32773 |
PRIDEi | P32773 |
TopDownProteomicsi | P32773 |
Genome annotation databases
EnsemblFungii | YOR194C_mRNA; YOR194C; YOR194C |
GeneIDi | 854369 |
KEGGi | sce:YOR194C |
Organism-specific databases
SGDi | S000005720, TOA1 |
VEuPathDBi | FungiDB:YOR194C |
Phylogenomic databases
eggNOGi | KOG2652, Eukaryota |
GeneTreei | ENSGT00940000169791 |
HOGENOMi | CLU_030027_2_1_1 |
InParanoidi | P32773 |
OMAi | RTKARWK |
Enzyme and pathway databases
Reactomei | R-SCE-674695, RNA Polymerase II Pre-transcription Events R-SCE-6807505, RNA polymerase II transcribes snRNA genes R-SCE-73776, RNA Polymerase II Promoter Escape R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-SCE-75953, RNA Polymerase II Transcription Initiation R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-SCE-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
EvolutionaryTracei | P32773 |
PROi | PR:P32773 |
RNActi | P32773, protein |
Family and domain databases
DisProti | DP00104 |
Gene3Di | 2.30.18.10, 1 hit |
InterProi | View protein in InterPro IPR004855, TFIIA_asu/bsu IPR009088, TFIIA_b-brl |
PANTHERi | PTHR12694, PTHR12694, 1 hit |
Pfami | View protein in Pfam PF03153, TFIIA, 2 hits |
SMARTi | View protein in SMART SM01371, TFIIA, 1 hit |
SUPFAMi | SSF50784, SSF50784, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TOA1_YEAST | |
Accessioni | P32773Primary (citable) accession number: P32773 Secondary accession number(s): D6W2Q3, E9P936 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 182 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families