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Entry version 179 (07 Apr 2021)
Sequence version 1 (01 Oct 1993)
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Protein

Transcription initiation factor IIA large subunit

Gene

TOA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

TFIIA is a component of the transcription machinery of RNA polymerase II and implicated in the regulation of basal transcription. Interacts with TBP (the TATA-binding protein).

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processTranscription

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-674695, RNA Polymerase II Pre-transcription Events
R-SCE-6807505, RNA polymerase II transcribes snRNA genes
R-SCE-73776, RNA Polymerase II Promoter Escape
R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953, RNA Polymerase II Transcription Initiation
R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SCE-9018519, Estrogen-dependent gene expression

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Transcription initiation factor IIA large subunit
Short name:
TFIIA large subunit
Alternative name(s):
TFIIA 32 kDa subunit
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:TOA1
Ordered Locus Names:YOR194C
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XV

Organism-specific databases

Saccharomyces Genome Database

More...
SGDi
S000005720, TOA1

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:YOR194C

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000726171 – 286Transcription initiation factor IIA large subunitAdd BLAST286

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P32773

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P32773

PRoteomics IDEntifications database

More...
PRIDEi
P32773

Consortium for Top Down Proteomics

More...
TopDownProteomicsi
P32773

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P32773

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

TFIIA is a heterodimer composed of the large TOA1 and a small TOA2 subunits.

Interacts with KAP122.

1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
34592, 274 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-1633, Transcription factor TFIIA complex

Database of interacting proteins

More...
DIPi
DIP-2347N

Protein interaction database and analysis system

More...
IntActi
P32773, 2 interactors

Molecular INTeraction database

More...
MINTi
P32773

STRING: functional protein association networks

More...
STRINGi
4932.YOR194C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P32773, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P32773

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P32773

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the TFIIA subunit 1 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2652, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000169791

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_030027_2_1_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P32773

Identification of Orthologs from Complete Genome Data

More...
OMAi
RTKARWK

Family and domain databases

Database of protein disorder

More...
DisProti
DP00104

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.30.18.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR004855, TFIIA_asu/bsu
IPR009088, TFIIA_b-brl

The PANTHER Classification System

More...
PANTHERi
PTHR12694, PTHR12694, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03153, TFIIA, 2 hits

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01371, TFIIA, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50784, SSF50784, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P32773-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSNAEASRVY EIIVESVVNE VREDFENAGI DEQTLQDLKN IWQKKLTETK
60 70 80 90 100
VTTFSWDNQF NEGNINGVQN DLNFNLATPG VNSSEFNIKE ENTGNEGLIL
110 120 130 140 150
PNINSNNNIP HSGETNINTN TVEATNNSGA TLNTNTSGNT NADVTSQPKI
160 170 180 190 200
EVKPEIELTI NNANITTVEN IDDESEKKDD EEKEEDVEKT RKEKEQIEQV
210 220 230 240 250
KLQAKKEKRS ALLDTDEVGS ELDDSDDDYL ISEGEEDGPD ENLMLCLYDK
260 270 280
VTRTKARWKC SLKDGVVTIN RNDYTFQKAQ VEAEWV
Length:286
Mass (Da):32,201
Last modified:October 1, 1993 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i5BF0C39581866C98
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti15E → Q in AAT93242 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M85248 Unassigned DNA Translation: AAA19654.1
Z75102 Genomic DNA Translation: CAA99407.1
AY693223 Genomic DNA Translation: AAT93242.1
BK006948 Genomic DNA Translation: DAA10969.1

Protein sequence database of the Protein Information Resource

More...
PIRi
B41810

NCBI Reference Sequences

More...
RefSeqi
NP_014837.1, NM_001183613.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YOR194C_mRNA; YOR194C; YOR194C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
854369

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YOR194C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85248 Unassigned DNA Translation: AAA19654.1
Z75102 Genomic DNA Translation: CAA99407.1
AY693223 Genomic DNA Translation: AAT93242.1
BK006948 Genomic DNA Translation: DAA10969.1
PIRiB41810
RefSeqiNP_014837.1, NM_001183613.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NH2X-ray1.90B2-54[»]
C210-286[»]
1RM1X-ray2.50C1-286[»]
1YTFX-ray2.50B2-54[»]
C210-286[»]
5FMFelectron microscopy6.00M2-286[»]
5FYWelectron microscopy4.35U1-286[»]
5FZ5electron microscopy8.80U1-286[»]
5OQJelectron microscopy4.70U1-286[»]
5OQMelectron microscopy5.80U1-286[»]
5SVAelectron microscopy15.30d1-286[»]
6GYKelectron microscopy5.10U1-286[»]
6GYLelectron microscopy4.80U1-286[»]
6GYMelectron microscopy6.70U1-286[»]
SMRiP32773
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi34592, 274 interactors
ComplexPortaliCPX-1633, Transcription factor TFIIA complex
DIPiDIP-2347N
IntActiP32773, 2 interactors
MINTiP32773
STRINGi4932.YOR194C

PTM databases

iPTMnetiP32773

Proteomic databases

MaxQBiP32773
PaxDbiP32773
PRIDEiP32773
TopDownProteomicsiP32773

Genome annotation databases

EnsemblFungiiYOR194C_mRNA; YOR194C; YOR194C
GeneIDi854369
KEGGisce:YOR194C

Organism-specific databases

SGDiS000005720, TOA1
VEuPathDBiFungiDB:YOR194C

Phylogenomic databases

eggNOGiKOG2652, Eukaryota
GeneTreeiENSGT00940000169791
HOGENOMiCLU_030027_2_1_1
InParanoidiP32773
OMAiRTKARWK

Enzyme and pathway databases

ReactomeiR-SCE-674695, RNA Polymerase II Pre-transcription Events
R-SCE-6807505, RNA polymerase II transcribes snRNA genes
R-SCE-73776, RNA Polymerase II Promoter Escape
R-SCE-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953, RNA Polymerase II Transcription Initiation
R-SCE-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SCE-9018519, Estrogen-dependent gene expression

Miscellaneous databases

EvolutionaryTraceiP32773

Protein Ontology

More...
PROi
PR:P32773
RNActiP32773, protein

Family and domain databases

DisProtiDP00104
Gene3Di2.30.18.10, 1 hit
InterProiView protein in InterPro
IPR004855, TFIIA_asu/bsu
IPR009088, TFIIA_b-brl
PANTHERiPTHR12694, PTHR12694, 1 hit
PfamiView protein in Pfam
PF03153, TFIIA, 2 hits
SMARTiView protein in SMART
SM01371, TFIIA, 1 hit
SUPFAMiSSF50784, SSF50784, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTOA1_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P32773
Secondary accession number(s): D6W2Q3, E9P936
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 7, 2021
This is version 179 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
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