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UniProtKB - P32727 (NUSA_BACSU)
Protein
Transcription termination/antitermination protein NusA
Gene
nusA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Participates in both transcription termination and antitermination.
UniRule annotationGO - Molecular functioni
- DNA-binding transcription factor activity Source: InterPro
- RNA binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA-templated transcription, termination Source: UniProtKB-UniRule
- transcription antitermination Source: GO_Central
Keywordsi
Molecular function | RNA-binding |
Biological process | Transcription, Transcription antitermination, Transcription regulation, Transcription termination |
Enzyme and pathway databases
BioCyci | BSUB:BSU16600-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription termination/antitermination protein NusAUniRule annotation |
Gene namesi | Name:nusAUniRule annotation Ordered Locus Names:BSU16600 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000181960 | 1 – 371 | Transcription termination/antitermination protein NusAAdd BLAST | 371 |
Proteomic databases
jPOSTi | P32727 |
PaxDbi | P32727 |
PRIDEi | P32727 |
Interactioni
Subunit structurei
Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.
UniRule annotationProtein-protein interaction databases
IntActi | P32727, 1 interactor |
MINTi | P32727 |
STRINGi | 224308.BSU16600 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P32727 |
BMRBi | P32727 |
SMRi | P32727 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 135 – 199 | S1 motifUniRule annotationAdd BLAST | 65 | |
Domaini | 301 – 367 | KHUniRule annotationAdd BLAST | 67 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 347 – 371 | DisorderedSequence analysisAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 356 – 371 | Acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Belongs to the NusA family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0195, Bacteria |
InParanoidi | P32727 |
OMAi | MDIDMSA |
PhylomeDBi | P32727 |
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit 3.30.1480.10, 1 hit 3.30.300.20, 2 hits |
HAMAPi | MF_00945_B, NusA_B, 1 hit |
InterProi | View protein in InterPro IPR004087, KH_dom IPR015946, KH_dom-like_a/b IPR025249, KH_dom_NusA-like IPR009019, KH_sf_prok-type IPR012340, NA-bd_OB-fold IPR030842, NusA_bac IPR036555, NusA_N_sf IPR022967, S1_dom IPR003029, S1_domain IPR013735, TF_NusA_N IPR010213, Tscrpt_termination_fac_NusA |
PANTHERi | PTHR22648, PTHR22648, 1 hit |
Pfami | View protein in Pfam PF13184, KH_5, 1 hit PF08529, NusA_N, 1 hit PF00575, S1, 1 hit |
SMARTi | View protein in SMART SM00322, KH, 2 hits SM00316, S1, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF54814, SSF54814, 2 hits SSF69705, SSF69705, 1 hit |
TIGRFAMsi | TIGR01953, NusA, 1 hit |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 1 hit PS50126, S1, 1 hit |
i Sequence
Sequence statusi: Complete.
P32727-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSELLDALT ILEKEKGISK EIIIEAIEAA LISAYKRNFN QAQNVRVDLN
60 70 80 90 100
RETGSIRVFA RKDVVDEVYD QRLEISIEEA QGIHPEYMVG DVVEIEVTPK
110 120 130 140 150
DFGRIAAQTA KQVVTQRVRE AERGVIYSEF IDREEDIMTG IVQRLDNKFI
160 170 180 190 200
YVSLGKIEAL LPVNEQMPNE SYKPHDRIKV YITKVEKTTK GPQIYVSRTH
210 220 230 240 250
PGLLKRLFEI EVPEIYDGTV ELKSVAREAG DRSKISVRTD DPDVDPVGSC
260 270 280 290 300
VGPKGQRVQA IVNELKGEKI DIVNWSSDPV EFVANALSPS KVLDVIVNEE
310 320 330 340 350
EKATTVIVPD YQLSLAIGKR GQNARLAAKL TGWKIDIKSE TDARELGIYP
360 370
RELEEDDEPL FTEPETAESD E
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 111 – 112 | KQ → NE in CAA79231 (PubMed:8491709).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z18631 Genomic DNA Translation: CAA79231.1 AL009126 Genomic DNA Translation: CAB13533.1 |
PIRi | C36905 |
RefSeqi | NP_389542.1, NC_000964.3 WP_003231912.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB13533; CAB13533; BSU_16600 |
GeneIDi | 939628 |
KEGGi | bsu:BSU16600 |
PATRICi | fig|224308.179.peg.1801 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z18631 Genomic DNA Translation: CAA79231.1 AL009126 Genomic DNA Translation: CAB13533.1 |
PIRi | C36905 |
RefSeqi | NP_389542.1, NC_000964.3 WP_003231912.1, NZ_JNCM01000035.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MT4 | NMR | - | A | 1-124 | [»] | |
AlphaFoldDBi | P32727 | |||||
BMRBi | P32727 | |||||
SMRi | P32727 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P32727, 1 interactor |
MINTi | P32727 |
STRINGi | 224308.BSU16600 |
Proteomic databases
jPOSTi | P32727 |
PaxDbi | P32727 |
PRIDEi | P32727 |
Genome annotation databases
EnsemblBacteriai | CAB13533; CAB13533; BSU_16600 |
GeneIDi | 939628 |
KEGGi | bsu:BSU16600 |
PATRICi | fig|224308.179.peg.1801 |
Phylogenomic databases
eggNOGi | COG0195, Bacteria |
InParanoidi | P32727 |
OMAi | MDIDMSA |
PhylomeDBi | P32727 |
Enzyme and pathway databases
BioCyci | BSUB:BSU16600-MONOMER |
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit 3.30.1480.10, 1 hit 3.30.300.20, 2 hits |
HAMAPi | MF_00945_B, NusA_B, 1 hit |
InterProi | View protein in InterPro IPR004087, KH_dom IPR015946, KH_dom-like_a/b IPR025249, KH_dom_NusA-like IPR009019, KH_sf_prok-type IPR012340, NA-bd_OB-fold IPR030842, NusA_bac IPR036555, NusA_N_sf IPR022967, S1_dom IPR003029, S1_domain IPR013735, TF_NusA_N IPR010213, Tscrpt_termination_fac_NusA |
PANTHERi | PTHR22648, PTHR22648, 1 hit |
Pfami | View protein in Pfam PF13184, KH_5, 1 hit PF08529, NusA_N, 1 hit PF00575, S1, 1 hit |
SMARTi | View protein in SMART SM00322, KH, 2 hits SM00316, S1, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF54814, SSF54814, 2 hits SSF69705, SSF69705, 1 hit |
TIGRFAMsi | TIGR01953, NusA, 1 hit |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 1 hit PS50126, S1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NUSA_BACSU | |
Accessioni | P32727Primary (citable) accession number: P32727 Secondary accession number(s): O31756 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | May 30, 2000 | |
Last modified: | May 25, 2022 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families