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UniProtKB - P32727 (NUSA_BACSU)

Entry version 155 (25 May 2022)
Sequence version 2 (30 May 2000)
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Protein

Transcription termination/antitermination protein NusA

Gene

nusA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Participates in both transcription termination and antitermination.

UniRule annotation

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionRNA-binding
Biological processTranscription, Transcription antitermination, Transcription regulation, Transcription termination

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		BSUB:BSU16600-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Transcription termination/antitermination protein NusAUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nusAUniRule annotation
Ordered Locus Names:BSU16600
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001819601 – 371Transcription termination/antitermination protein NusAAdd BLAST371

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P32727

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P32727

PRoteomics IDEntifications database

More...PRIDEi		P32727

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.

UniRule annotation

Protein-protein interaction databases

Protein interaction database and analysis system

More...IntActi		P32727, 1 interactor

Molecular INTeraction database

More...MINTi		P32727

STRING: functional protein association networks

More...STRINGi		224308.BSU16600

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1371
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		P32727

Biological Magnetic Resonance Data Bank

More...BMRBi		P32727

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P32727

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini135 – 199S1 motifUniRule annotationAdd BLAST65
Domaini301 – 367KHUniRule annotationAdd BLAST67

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni347 – 371DisorderedSequence analysisAdd BLAST25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi356 – 371Acidic residuesSequence analysisAdd BLAST16

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the NusA family.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0195, Bacteria

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P32727

Identification of Orthologs from Complete Genome Data

More...OMAi		MDIDMSA

Database for complete collections of gene phylogenies

More...PhylomeDBi		P32727

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		2.40.50.140, 1 hit
3.30.1480.10, 1 hit
3.30.300.20, 2 hits

HAMAP database of protein families

More...HAMAPi		MF_00945_B, NusA_B, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR004087, KH_dom
IPR015946, KH_dom-like_a/b
IPR025249, KH_dom_NusA-like
IPR009019, KH_sf_prok-type
IPR012340, NA-bd_OB-fold
IPR030842, NusA_bac
IPR036555, NusA_N_sf
IPR022967, S1_dom
IPR003029, S1_domain
IPR013735, TF_NusA_N
IPR010213, Tscrpt_termination_fac_NusA

The PANTHER Classification System

More...PANTHERi		PTHR22648, PTHR22648, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF13184, KH_5, 1 hit
PF08529, NusA_N, 1 hit
PF00575, S1, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00322, KH, 2 hits
SM00316, S1, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF50249, SSF50249, 1 hit
SSF54814, SSF54814, 2 hits
SSF69705, SSF69705, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR01953, NusA, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50084, KH_TYPE_1, 1 hit
PS50126, S1, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P32727-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSELLDALT ILEKEKGISK EIIIEAIEAA LISAYKRNFN QAQNVRVDLN 
        60         70         80         90        100
RETGSIRVFA RKDVVDEVYD QRLEISIEEA QGIHPEYMVG DVVEIEVTPK 
       110        120        130        140        150
DFGRIAAQTA KQVVTQRVRE AERGVIYSEF IDREEDIMTG IVQRLDNKFI 
       160        170        180        190        200
YVSLGKIEAL LPVNEQMPNE SYKPHDRIKV YITKVEKTTK GPQIYVSRTH 
       210        220        230        240        250
PGLLKRLFEI EVPEIYDGTV ELKSVAREAG DRSKISVRTD DPDVDPVGSC 
       260        270        280        290        300
VGPKGQRVQA IVNELKGEKI DIVNWSSDPV EFVANALSPS KVLDVIVNEE 
       310        320        330        340        350
EKATTVIVPD YQLSLAIGKR GQNARLAAKL TGWKIDIKSE TDARELGIYP 
       360        370 
RELEEDDEPL FTEPETAESD E
Length:371
Mass (Da):41,725
Last modified:May 30, 2000 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i2FC7861E4C3094D3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti111 – 112KQ → NE in CAA79231 (PubMed:8491709).Curated2

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
Z18631 Genomic DNA Translation: CAA79231.1
AL009126 Genomic DNA Translation: CAB13533.1

Protein sequence database of the Protein Information Resource

More...PIRi		C36905

NCBI Reference Sequences

More...RefSeqi		NP_389542.1, NC_000964.3
WP_003231912.1, NZ_JNCM01000035.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		CAB13533; CAB13533; BSU_16600

Database of genes from NCBI RefSeq genomes

More...GeneIDi		939628

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		bsu:BSU16600

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|224308.179.peg.1801

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18631 Genomic DNA Translation: CAA79231.1
AL009126 Genomic DNA Translation: CAB13533.1
PIRiC36905
RefSeqiNP_389542.1, NC_000964.3
WP_003231912.1, NZ_JNCM01000035.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MT4NMR-A1-124[»]
AlphaFoldDBiP32727
BMRBiP32727
SMRiP32727
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

IntActiP32727, 1 interactor
MINTiP32727
STRINGi224308.BSU16600

Proteomic databases

jPOSTiP32727
PaxDbiP32727
PRIDEiP32727

Genome annotation databases

EnsemblBacteriaiCAB13533; CAB13533; BSU_16600
GeneIDi939628
KEGGibsu:BSU16600
PATRICifig|224308.179.peg.1801

Phylogenomic databases

eggNOGiCOG0195, Bacteria
InParanoidiP32727
OMAiMDIDMSA
PhylomeDBiP32727

Enzyme and pathway databases

BioCyciBSUB:BSU16600-MONOMER

Family and domain databases

Gene3Di2.40.50.140, 1 hit
3.30.1480.10, 1 hit
3.30.300.20, 2 hits
HAMAPiMF_00945_B, NusA_B, 1 hit
InterProiView protein in InterPro
IPR004087, KH_dom
IPR015946, KH_dom-like_a/b
IPR025249, KH_dom_NusA-like
IPR009019, KH_sf_prok-type
IPR012340, NA-bd_OB-fold
IPR030842, NusA_bac
IPR036555, NusA_N_sf
IPR022967, S1_dom
IPR003029, S1_domain
IPR013735, TF_NusA_N
IPR010213, Tscrpt_termination_fac_NusA
PANTHERiPTHR22648, PTHR22648, 1 hit
PfamiView protein in Pfam
PF13184, KH_5, 1 hit
PF08529, NusA_N, 1 hit
PF00575, S1, 1 hit
SMARTiView protein in SMART
SM00322, KH, 2 hits
SM00316, S1, 1 hit
SUPFAMiSSF50249, SSF50249, 1 hit
SSF54814, SSF54814, 2 hits
SSF69705, SSF69705, 1 hit
TIGRFAMsiTIGR01953, NusA, 1 hit
PROSITEiView protein in PROSITE
PS50084, KH_TYPE_1, 1 hit
PS50126, S1, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNUSA_BACSU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P32727
Secondary accession number(s): O31756
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: May 30, 2000
Last modified: May 25, 2022
This is version 155 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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