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Entry version 139 (02 Dec 2020)
Sequence version 2 (29 Aug 2003)
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Protein

Probable cytochrome c-type biogenesis protein NrfE

Gene

nrfE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • cytochrome c-heme linkage via heme-L-cysteine Source: EcoCyc

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processCytochrome c-type biogenesis

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:EG11948-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Probable cytochrome c-type biogenesis protein NrfE
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nrfE
Synonyms:yjcL
Ordered Locus Names:b4074, JW4035
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei26 – 46HelicalSequence analysisAdd BLAST21
Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Transmembranei228 – 247HelicalSequence analysisAdd BLAST20
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Transmembranei294 – 314HelicalSequence analysisAdd BLAST21
Transmembranei333 – 353HelicalSequence analysisAdd BLAST21
Transmembranei376 – 396HelicalSequence analysisAdd BLAST21
Transmembranei399 – 419HelicalSequence analysisAdd BLAST21
Transmembranei525 – 545HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Triple nrfE-nrfF-nrfG deletion mutants no longer attach heme 1 (covalently attached to 'Cys-122', the CWSCK motif) of cytochrome c552 (AC P0ABK9).1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002015891 – 552Probable cytochrome c-type biogenesis protein NrfEAdd BLAST552

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P32710

PRoteomics IDEntifications database

More...
PRIDEi
P32710

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

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BioGRIDi
4262677, 7 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b4074

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG1138, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_015041_3_0_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P32710

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P32710

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR032523, CcmF_C
IPR002541, Cyt_c_assembly
IPR003567, Cyt_c_biogenesis
IPR003568, Cyt_c_biogenesis_CcmF
IPR003570, Cyt_c_biogenesis_NrfE

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF16327, CcmF_C, 2 hits
PF01578, Cytochrom_C_asm, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR01410, CCBIOGENESIS
PR01413, NRFEBIOGNSIS

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00353, nrfE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P32710-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLTPLTAFAG VRLRWPAMMR LTCIGILAQF ALLLLAFGVL TYCFLISDFS
60 70 80 90 100
VIYVAQHSYS LLSWELKLAA VWGGHEGSLL LWVLLLSAWS ALFAWHYRQQ
110 120 130 140 150
TDPLFPLTLA VLSLMLAALL LFVVLWSDPF VRIFPPAIEG RDLNPMLQHP
160 170 180 190 200
GLIFHPPLLY LGYGGLMVAA SVALASLLRG EFDGACARIC WRWALPGWSA
210 220 230 240 250
LTAGIILGSW WAYCELGWGG WWFWDPVENA SLLPWLSATA LLHSLSLTRQ
260 270 280 290 300
RGIFCHWSLL LAIVTLMLSL LGTLIVRSGI LVSVHAFALD NVRAVPLFSL
310 320 330 340 350
FALISLASLA LYGWRARDGG PAVHFSGLSR EMLILATLLL FCAVLLIVLV
360 370 380 390 400
GTLYPMIYGL LGWGRLSVGA PYFNRATLPF GLLMLVVIVL ATFVSGKRVQ
410 420 430 440 450
LPALVAHAGV LLFAAGVVVS SVSRQEISLN LQPGQQVTLA GYTFRFECLD
460 470 480 490 500
LQAKGNYTSE KAIVALFDHQ QRIGELTPER RFYEARRQQM MEPSIRWNGI
510 520 530 540 550
HDWYAVMGEK TGPDRYAFRL YVQSGVRWIW GGGLLMIAGA LLSGWRGKKR

DE
Length:552
Mass (Da):61,295
Last modified:August 29, 2003 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i138C2E21933699E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti32L → V in CAA51045 (PubMed:8057835).Curated1
Sequence conflicti38G → A in CAA51045 (PubMed:8057835).Curated1
Sequence conflicti190C → S in CAA51045 (PubMed:8057835).Curated1
Sequence conflicti218W → L in CAA51045 (PubMed:8057835).Curated1
Sequence conflicti220 – 226GWWFWDP → RLVVLGS in CAA51045 (PubMed:8057835).Curated7
Sequence conflicti455G → A in CAA51045 (PubMed:8057835).Curated1
Sequence conflicti481R → A in CAA51045 (PubMed:8057835).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X72298 Genomic DNA Translation: CAA51045.1
U00006 Genomic DNA Translation: AAC43168.1
U00096 Genomic DNA Translation: AAD13457.1
AP009048 Genomic DNA Translation: BAE78076.1

Protein sequence database of the Protein Information Resource

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PIRi
A65216, E57987

NCBI Reference Sequences

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RefSeqi
NP_418498.1, NC_000913.3

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAD13457; AAD13457; b4074
BAE78076; BAE78076; BAE78076

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
948579

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW4035
eco:b4074

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|511145.12.peg.4197

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72298 Genomic DNA Translation: CAA51045.1
U00006 Genomic DNA Translation: AAC43168.1
U00096 Genomic DNA Translation: AAD13457.1
AP009048 Genomic DNA Translation: BAE78076.1
PIRiA65216, E57987
RefSeqiNP_418498.1, NC_000913.3

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi4262677, 7 interactors
STRINGi511145.b4074

Proteomic databases

PaxDbiP32710
PRIDEiP32710

Genome annotation databases

EnsemblBacteriaiAAD13457; AAD13457; b4074
BAE78076; BAE78076; BAE78076
GeneIDi948579
KEGGiecj:JW4035
eco:b4074
PATRICifig|511145.12.peg.4197

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB1891

Phylogenomic databases

eggNOGiCOG1138, Bacteria
HOGENOMiCLU_015041_3_0_6
InParanoidiP32710
PhylomeDBiP32710

Enzyme and pathway databases

BioCyciEcoCyc:EG11948-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P32710

Family and domain databases

InterProiView protein in InterPro
IPR032523, CcmF_C
IPR002541, Cyt_c_assembly
IPR003567, Cyt_c_biogenesis
IPR003568, Cyt_c_biogenesis_CcmF
IPR003570, Cyt_c_biogenesis_NrfE
PfamiView protein in Pfam
PF16327, CcmF_C, 2 hits
PF01578, Cytochrom_C_asm, 1 hit
PRINTSiPR01410, CCBIOGENESIS
PR01413, NRFEBIOGNSIS
TIGRFAMsiTIGR00353, nrfE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNRFE_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P32710
Secondary accession number(s): Q2M6N0
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: August 29, 2003
Last modified: December 2, 2020
This is version 139 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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