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UniProtKB - P32710 (NRFE_ECOLI)
Protein
Probable cytochrome c-type biogenesis protein NrfE
Gene
nrfE
Organism
Escherichia coli (strain K12)
Status
Functioni
May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase.
1 PublicationGO - Molecular functioni
- heme binding Source: InterPro
- heme transmembrane transporter activity Source: InterPro
GO - Biological processi
- cytochrome c-heme linkage via heme-L-cysteine Source: EcoCyc
Keywordsi
Biological process | Cytochrome c-type biogenesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11948-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Probable cytochrome c-type biogenesis protein NrfE |
Gene namesi | Name:nrfE Synonyms:yjcL Ordered Locus Names:b4074, JW4035 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 26 – 46 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 77 – 97 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 105 – 125 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 158 – 178 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 193 – 213 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 228 – 247 | HelicalSequence analysisAdd BLAST | 20 | |
Transmembranei | 256 – 276 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 294 – 314 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 333 – 353 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 376 – 396 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 399 – 419 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 525 – 545 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Triple nrfE-nrfF-nrfG deletion mutants no longer attach heme 1 (covalently attached to 'Cys-122', the CWSCK motif) of cytochrome c552 (AC P0ABK9).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201589 | 1 – 552 | Probable cytochrome c-type biogenesis protein NrfEAdd BLAST | 552 |
Proteomic databases
PaxDbi | P32710 |
PRIDEi | P32710 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262677, 7 interactors |
STRINGi | 511145.b4074 |
Family & Domainsi
Sequence similaritiesi
Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1138, Bacteria |
HOGENOMi | CLU_015041_3_0_6 |
InParanoidi | P32710 |
PhylomeDBi | P32710 |
Family and domain databases
InterProi | View protein in InterPro IPR032523, CcmF_C IPR002541, Cyt_c_assembly IPR003567, Cyt_c_biogenesis IPR003568, Cyt_c_biogenesis_CcmF IPR003570, Cyt_c_biogenesis_NrfE |
PANTHERi | PTHR43653, PTHR43653, 1 hit |
Pfami | View protein in Pfam PF16327, CcmF_C, 2 hits PF01578, Cytochrom_C_asm, 1 hit |
PRINTSi | PR01410, CCBIOGENESIS PR01413, NRFEBIOGNSIS |
TIGRFAMsi | TIGR00353, nrfE, 1 hit |
i Sequence
Sequence statusi: Complete.
P32710-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLTPLTAFAG VRLRWPAMMR LTCIGILAQF ALLLLAFGVL TYCFLISDFS
60 70 80 90 100
VIYVAQHSYS LLSWELKLAA VWGGHEGSLL LWVLLLSAWS ALFAWHYRQQ
110 120 130 140 150
TDPLFPLTLA VLSLMLAALL LFVVLWSDPF VRIFPPAIEG RDLNPMLQHP
160 170 180 190 200
GLIFHPPLLY LGYGGLMVAA SVALASLLRG EFDGACARIC WRWALPGWSA
210 220 230 240 250
LTAGIILGSW WAYCELGWGG WWFWDPVENA SLLPWLSATA LLHSLSLTRQ
260 270 280 290 300
RGIFCHWSLL LAIVTLMLSL LGTLIVRSGI LVSVHAFALD NVRAVPLFSL
310 320 330 340 350
FALISLASLA LYGWRARDGG PAVHFSGLSR EMLILATLLL FCAVLLIVLV
360 370 380 390 400
GTLYPMIYGL LGWGRLSVGA PYFNRATLPF GLLMLVVIVL ATFVSGKRVQ
410 420 430 440 450
LPALVAHAGV LLFAAGVVVS SVSRQEISLN LQPGQQVTLA GYTFRFECLD
460 470 480 490 500
LQAKGNYTSE KAIVALFDHQ QRIGELTPER RFYEARRQQM MEPSIRWNGI
510 520 530 540 550
HDWYAVMGEK TGPDRYAFRL YVQSGVRWIW GGGLLMIAGA LLSGWRGKKR
DE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 32 | L → V in CAA51045 (PubMed:8057835).Curated | 1 | |
Sequence conflicti | 38 | G → A in CAA51045 (PubMed:8057835).Curated | 1 | |
Sequence conflicti | 190 | C → S in CAA51045 (PubMed:8057835).Curated | 1 | |
Sequence conflicti | 218 | W → L in CAA51045 (PubMed:8057835).Curated | 1 | |
Sequence conflicti | 220 – 226 | GWWFWDP → RLVVLGS in CAA51045 (PubMed:8057835).Curated | 7 | |
Sequence conflicti | 455 | G → A in CAA51045 (PubMed:8057835).Curated | 1 | |
Sequence conflicti | 481 | R → A in CAA51045 (PubMed:8057835).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72298 Genomic DNA Translation: CAA51045.1 U00006 Genomic DNA Translation: AAC43168.1 U00096 Genomic DNA Translation: AAD13457.1 AP009048 Genomic DNA Translation: BAE78076.1 |
PIRi | A65216, E57987 |
RefSeqi | NP_418498.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAD13457; AAD13457; b4074 BAE78076; BAE78076; BAE78076 |
GeneIDi | 948579 |
KEGGi | ecj:JW4035 eco:b4074 |
PATRICi | fig|511145.12.peg.4197 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72298 Genomic DNA Translation: CAA51045.1 U00006 Genomic DNA Translation: AAC43168.1 U00096 Genomic DNA Translation: AAD13457.1 AP009048 Genomic DNA Translation: BAE78076.1 |
PIRi | A65216, E57987 |
RefSeqi | NP_418498.1, NC_000913.3 |
3D structure databases
SMRi | P32710 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262677, 7 interactors |
STRINGi | 511145.b4074 |
Proteomic databases
PaxDbi | P32710 |
PRIDEi | P32710 |
Genome annotation databases
EnsemblBacteriai | AAD13457; AAD13457; b4074 BAE78076; BAE78076; BAE78076 |
GeneIDi | 948579 |
KEGGi | ecj:JW4035 eco:b4074 |
PATRICi | fig|511145.12.peg.4197 |
Organism-specific databases
EchoBASEi | EB1891 |
Phylogenomic databases
eggNOGi | COG1138, Bacteria |
HOGENOMi | CLU_015041_3_0_6 |
InParanoidi | P32710 |
PhylomeDBi | P32710 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11948-MONOMER |
Miscellaneous databases
PROi | PR:P32710 |
Family and domain databases
InterProi | View protein in InterPro IPR032523, CcmF_C IPR002541, Cyt_c_assembly IPR003567, Cyt_c_biogenesis IPR003568, Cyt_c_biogenesis_CcmF IPR003570, Cyt_c_biogenesis_NrfE |
PANTHERi | PTHR43653, PTHR43653, 1 hit |
Pfami | View protein in Pfam PF16327, CcmF_C, 2 hits PF01578, Cytochrom_C_asm, 1 hit |
PRINTSi | PR01410, CCBIOGENESIS PR01413, NRFEBIOGNSIS |
TIGRFAMsi | TIGR00353, nrfE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NRFE_ECOLI | |
Accessioni | P32710Primary (citable) accession number: P32710 Secondary accession number(s): Q2M6N0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | August 29, 2003 | |
Last modified: | February 23, 2022 | |
This is version 141 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families