UniProtKB - P32684 (RLUF_ECOLI)
Protein
Dual-specificity RNA pseudouridine synthase RluF
Gene
rluF
Organism
Escherichia coli (strain K12)
Status
Functioni
Dual specificity enzyme that catalyzes the synthesis of pseudouridine from uracil-2604 in 23S ribosomal RNA and from uracil-35 in the anticodon of tRNA(Tyr) (PubMed:11720289, PubMed:27551044). Can, to a small extent, also react with uracil-2605 (PubMed:11720289).2 Publications
Catalytic activityi
- EC:5.4.99.213 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 107 | Nucleophile1 Publication | 1 |
GO - Molecular functioni
- pseudouridine synthase activity Source: EcoCyc
- RNA binding Source: InterPro
- rRNA pseudouridine synthase activity Source: EcoCyc
- tRNA pseudouridine synthase activity Source: EcoCyc
GO - Biological processi
- enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
- tRNA pseudouridine synthesis Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Biological process | rRNA processing, tRNA processing |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11921-MONOMER MetaCyc:EG11921-MONOMER |
BRENDAi | 5.4.99.21, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Dual-specificity RNA pseudouridine synthase RluFCurated (EC:5.4.99.-1 Publication, EC:5.4.99.213 Publications)Alternative name(s): 23S rRNA pseudouridine(2604) synthase1 Publication Ribosomal large subunit pseudouridine synthase FCurated rRNA pseudouridylate synthase FCurated rRNA-uridine isomerase FCurated tRNA(Tyr) pseudouridine(35) synthaseCurated |
Gene namesi | Name:rluF Synonyms:yjbC Ordered Locus Names:b4022, JW3982 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 107 | D → N or T: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100016 | 1 – 290 | Dual-specificity RNA pseudouridine synthase RluFAdd BLAST | 290 |
Proteomic databases
jPOSTi | P32684 |
PaxDbi | P32684 |
PRIDEi | P32684 |
Interactioni
Subunit structurei
Monomer.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4261777, 60 interactors |
IntActi | P32684, 9 interactors |
STRINGi | 511145.b4022 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P32684 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P32684 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 72 | S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST | 66 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 105 – 108 | Interaction with RNA1 Publication | 4 | |
Regioni | 187 – 190 | Interaction with RNA1 Publication | 4 |
Sequence similaritiesi
Belongs to the pseudouridine synthase RsuA family.Curated
Phylogenomic databases
eggNOGi | COG1187, Bacteria |
HOGENOMi | CLU_024979_6_1_6 |
InParanoidi | P32684 |
PhylomeDBi | P32684 |
Family and domain databases
CDDi | cd00165, S4, 1 hit |
DisProti | DP02011 |
Gene3Di | 3.10.290.10, 1 hit 3.30.70.1560, 1 hit |
InterProi | View protein in InterPro IPR042092, PsdUridine_s_RsuA/RluB/E/F_cat IPR020103, PsdUridine_synth_cat_dom_sf IPR006145, PsdUridine_synth_RsuA/RluA IPR000748, PsdUridine_synth_RsuA/RluB/E/F IPR018496, PsdUridine_synth_RsuA/RluB_CS IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit PF01479, S4, 1 hit |
SMARTi | View protein in SMART SM00363, S4, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00093, TIGR00093, 1 hit |
PROSITEi | View protein in PROSITE PS01149, PSI_RSU, 1 hit PS50889, S4, 1 hit |
i Sequence
Sequence statusi: Complete.
P32684-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLPDSSVRLN KYISESGICS RREADRYIEQ GNVFLNGKRA TIGDQVKPGD
60 70 80 90 100
VVKVNGQLIE PREAEDLVLI ALNKPVGIVS TTEDGERDNI VDFVNHSKRV
110 120 130 140 150
FPIGRLDKDS QGLIFLTNHG DLVNKILRAG NDHEKEYLVT VDKPITEEFI
160 170 180 190 200
RGMSAGVPIL GTVTKKCKVK KEAPFVFRIT LVQGLNRQIR RMCEHFGYEV
210 220 230 240 250
KKLERTRIMN VSLSGIPLGE WRDLTDDELI DLFKLIENSS SEVKPKAKAK
260 270 280 290
PKTAGIKRPV VKMEKTAEKG GRPASNGKRF TSPGRKKKGR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00006 Genomic DNA Translation: AAC43116.1 U00096 Genomic DNA Translation: AAC76992.1 AP009048 Genomic DNA Translation: BAE78024.1 |
PIRi | E65209 |
RefSeqi | NP_418446.1, NC_000913.3 WP_000936377.1, NZ_SSZK01000049.1 |
Genome annotation databases
EnsemblBacteriai | AAC76992; AAC76992; b4022 BAE78024; BAE78024; BAE78024 |
GeneIDi | 57730517 948519 |
KEGGi | ecj:JW3982 eco:b4022 |
PATRICi | fig|1411691.4.peg.2691 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00006 Genomic DNA Translation: AAC43116.1 U00096 Genomic DNA Translation: AAC76992.1 AP009048 Genomic DNA Translation: BAE78024.1 |
PIRi | E65209 |
RefSeqi | NP_418446.1, NC_000913.3 WP_000936377.1, NZ_SSZK01000049.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2GML | X-ray | 2.60 | A/B | 66-290 | [»] | |
3DH3 | X-ray | 3.00 | A/B/C/D | 1-290 | [»] | |
SMRi | P32684 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261777, 60 interactors |
IntActi | P32684, 9 interactors |
STRINGi | 511145.b4022 |
Proteomic databases
jPOSTi | P32684 |
PaxDbi | P32684 |
PRIDEi | P32684 |
Genome annotation databases
EnsemblBacteriai | AAC76992; AAC76992; b4022 BAE78024; BAE78024; BAE78024 |
GeneIDi | 57730517 948519 |
KEGGi | ecj:JW3982 eco:b4022 |
PATRICi | fig|1411691.4.peg.2691 |
Organism-specific databases
EchoBASEi | EB1865 |
Phylogenomic databases
eggNOGi | COG1187, Bacteria |
HOGENOMi | CLU_024979_6_1_6 |
InParanoidi | P32684 |
PhylomeDBi | P32684 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11921-MONOMER MetaCyc:EG11921-MONOMER |
BRENDAi | 5.4.99.21, 2026 |
Miscellaneous databases
EvolutionaryTracei | P32684 |
PROi | PR:P32684 |
Family and domain databases
CDDi | cd00165, S4, 1 hit |
DisProti | DP02011 |
Gene3Di | 3.10.290.10, 1 hit 3.30.70.1560, 1 hit |
InterProi | View protein in InterPro IPR042092, PsdUridine_s_RsuA/RluB/E/F_cat IPR020103, PsdUridine_synth_cat_dom_sf IPR006145, PsdUridine_synth_RsuA/RluA IPR000748, PsdUridine_synth_RsuA/RluB/E/F IPR018496, PsdUridine_synth_RsuA/RluB_CS IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf |
Pfami | View protein in Pfam PF00849, PseudoU_synth_2, 1 hit PF01479, S4, 1 hit |
SMARTi | View protein in SMART SM00363, S4, 1 hit |
SUPFAMi | SSF55120, SSF55120, 1 hit |
TIGRFAMsi | TIGR00093, TIGR00093, 1 hit |
PROSITEi | View protein in PROSITE PS01149, PSI_RSU, 1 hit PS50889, S4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RLUF_ECOLI | |
Accessioni | P32684Primary (citable) accession number: P32684 Secondary accession number(s): Q2M6T2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | October 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families