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Entry version 141 (02 Dec 2020)
Sequence version 1 (01 Oct 1993)
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Protein

Probable dehydratase PflD

Gene

pflD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Probably shows dehydratase activity.1 Publication

Miscellaneous

Contains a dehydratase motif that is predictive of enzyme function.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLyase

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:EG11910-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Probable dehydratase PflDCurated (EC:4.2.1.-Curated)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:pflD
Synonyms:yijL
Ordered Locus Names:b3951, JW3923
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001666891 – 765Probable dehydratase PflDAdd BLAST765

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei741Glycine radicalPROSITE-ProRule annotation1

Keywords - PTMi

Organic radical

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P32674

PRoteomics IDEntifications database

More...
PRIDEi
P32674

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4262065, 3 interactors

Database of interacting proteins

More...
DIPi
DIP-10469N

Protein interaction database and analysis system

More...
IntActi
P32674, 4 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b3951

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P32674

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini3 – 637PFLPROSITE-ProRule annotationAdd BLAST635
Domaini645 – 765Glycine radicalPROSITE-ProRule annotationAdd BLAST121

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the glycyl radical enzyme (GRE) family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG1882, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_009096_0_1_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P32674

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P32674

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR019777, Form_AcTrfase_GR_CS
IPR001150, Gly_radical
IPR010098, PFL2/GDeHydtase_fam
IPR004184, PFL_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01228, Gly_radical, 1 hit
PF02901, PFL-like, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01774, PFL2-3, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00850, GLY_RADICAL_1, 1 hit
PS51149, GLY_RADICAL_2, 1 hit
PS51554, PFL, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P32674-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTNRISRLKT ALFANTREIS LERALLYTAS HRQTEGEPVI LRRAKATAYI
60 70 80 90 100
LEHVEISIRD EELIAGNRTV KPRAGIMSPE MDPYWLLKEL DQFPTRPQDR
110 120 130 140 150
FAISEEDKRI YREELFPYWE KRSMKDFING QMTDEVKAAT NTQIFSINQT
160 170 180 190 200
DKGQGHIIID YPRLLNHGLG ELVAQMQQHC QQQPENHFYQ AALLLLEASQ
210 220 230 240 250
KHILRYAELA ETMAANCTDA QRREELLTIA EISRHNAQHK PQTFWQACQL
260 270 280 290 300
FWYMNIILQY ESNASSLSLG RFDQYMLPFY QTSLTQGEDA AFLKELLESL
310 320 330 340 350
WVKCNDIVLL RSTSSARYFA GFPTGYTALL GGLTENGRSA VNVLSFLCLD
360 370 380 390 400
AYQSVQLPQP NLGVRTNALI DTPFLMKTAE TIRFGTGIPQ IFNDEVVVPA
410 420 430 440 450
FLNRGVSLED ARDYSVVGCV ELSIPGRTYG LHDIAMFNLL KVMEICLHEN
460 470 480 490 500
EGNAALTYEG LLEQIRAKIS HYITLMVEGS NICDIGHRDW APVPLLSSFI
510 520 530 540 550
SDCLEKGRDI TDGGARYNFS GVQGIGIANL SDSLHALKGM VFEQQRLSFD
560 570 580 590 600
ELLSVLKANF ATPEGEKVRA RLINRFEKYG NDIDEVDNIS AELLRHYCKE
610 620 630 640 650
VEKYQNPRGG YFTPGSYTVS AHVPLGSVVG ATPDGRFAGE QLADGGLSPM
660 670 680 690 700
LGQDAQGPTA VLKSVSKLDN TLLSNGTLLN VKFTPATLEG EAGLRKLADF
710 720 730 740 750
LRAFTQLKLQ HIQFNVVNAD TLREAQQRPQ DYAGLVVRVA GYSAFFVELS
760
KEIQDDIIRR TAHQL
Length:765
Mass (Da):85,960
Last modified:October 1, 1993 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i369D6B6E1BBC5BA0
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U00006 Genomic DNA Translation: AAC43057.1
U00096 Genomic DNA Translation: AAC76933.1
AP009048 Genomic DNA Translation: BAE77360.1

Protein sequence database of the Protein Information Resource

More...
PIRi
B65202

NCBI Reference Sequences

More...
RefSeqi
NP_418386.1, NC_000913.3
WP_000184811.1, NZ_SSZK01000014.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC76933; AAC76933; b3951
BAE77360; BAE77360; BAE77360

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
948454

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW3923
eco:b3951

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.2754

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA Translation: AAC43057.1
U00096 Genomic DNA Translation: AAC76933.1
AP009048 Genomic DNA Translation: BAE77360.1
PIRiB65202
RefSeqiNP_418386.1, NC_000913.3
WP_000184811.1, NZ_SSZK01000014.1

3D structure databases

SMRiP32674
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4262065, 3 interactors
DIPiDIP-10469N
IntActiP32674, 4 interactors
STRINGi511145.b3951

Proteomic databases

PaxDbiP32674
PRIDEiP32674

Genome annotation databases

EnsemblBacteriaiAAC76933; AAC76933; b3951
BAE77360; BAE77360; BAE77360
GeneIDi948454
KEGGiecj:JW3923
eco:b3951
PATRICifig|1411691.4.peg.2754

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB1854

Phylogenomic databases

eggNOGiCOG1882, Bacteria
HOGENOMiCLU_009096_0_1_6
InParanoidiP32674
PhylomeDBiP32674

Enzyme and pathway databases

BioCyciEcoCyc:EG11910-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P32674

Family and domain databases

InterProiView protein in InterPro
IPR019777, Form_AcTrfase_GR_CS
IPR001150, Gly_radical
IPR010098, PFL2/GDeHydtase_fam
IPR004184, PFL_dom
PfamiView protein in Pfam
PF01228, Gly_radical, 1 hit
PF02901, PFL-like, 1 hit
TIGRFAMsiTIGR01774, PFL2-3, 1 hit
PROSITEiView protein in PROSITE
PS00850, GLY_RADICAL_1, 1 hit
PS51149, GLY_RADICAL_2, 1 hit
PS51554, PFL, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGRE1_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P32674
Secondary accession number(s): Q2M8P6
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: December 2, 2020
This is version 141 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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